FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n02_35005-1.3420000003578b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n02_35005-1.3420000003578b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2608703
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG61370.23525100404300528No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC56480.21650605684127322No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA28300.10848302777280511No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA28220.10817636196991379No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA26490.10154471398238896No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA6050.064.303052
GAGCGGC3750.058.1242879
CGGACCG250.00504356656.9382861
TCGACGC250.00504586856.931722
AGAGCGG4350.052.289738
CGGGAGA6000.051.3878064
TGCGGCC752.413799E-950.6059762
AAGCGGC2250.048.43699
GGTACGG502.8461522E-547.448571
CGACGCC405.4183026E-447.448571
TCGGGAA2200.045.282253
CGGAAAG2200.045.278775
AACCGGA1400.044.054312
GAACCGG1350.042.176511
GAGAGCG6150.041.6093947
ACACGGG704.3445216E-640.6702041
CGAGGCG704.3445216E-640.6702041
TTGTGTT325100.039.260741
GATCGGG11900.037.480381
CCGGATC3000.037.1977160-61
Produced by FastQC (version 0.11.2)