##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n02_34344-1.34200000035854.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4707634 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.703952346337886 33.0 33.0 33.0 27.0 33.0 2 31.590501088232433 33.0 33.0 33.0 27.0 33.0 3 31.52125356389218 33.0 33.0 33.0 27.0 33.0 4 35.13825692481616 37.0 37.0 37.0 33.0 37.0 5 35.15459060751112 37.0 37.0 37.0 33.0 37.0 6 35.26318719764536 37.0 37.0 37.0 33.0 37.0 7 35.04354714066557 37.0 37.0 37.0 33.0 37.0 8 35.176541336900875 37.0 37.0 37.0 33.0 37.0 9 34.9761880384074 37.0 37.0 37.0 33.0 37.0 10-11 34.90406061728673 37.0 37.0 37.0 33.0 37.0 12-13 34.88490194862217 37.0 37.0 37.0 33.0 37.0 14-15 36.498963279643235 40.0 37.0 40.0 33.0 40.0 16-17 36.43746741993961 40.0 37.0 40.0 33.0 40.0 18-19 36.45611819015667 40.0 37.0 40.0 33.0 40.0 20-21 36.46461247837024 40.0 37.0 40.0 33.0 40.0 22-23 36.618020750975965 40.0 37.0 40.0 33.0 40.0 24-25 36.19974513736625 40.0 37.0 40.0 30.0 40.0 26-27 36.142257979273666 40.0 37.0 40.0 27.0 40.0 28-29 36.06597358673168 40.0 37.0 40.0 27.0 40.0 30-31 36.025268956762574 40.0 37.0 40.0 27.0 40.0 32-33 35.920178161683765 40.0 37.0 40.0 27.0 40.0 34-35 35.89037136276949 38.5 37.0 40.0 27.0 40.0 36-37 35.77850922565348 37.0 37.0 40.0 27.0 40.0 38-39 35.64233105207414 37.0 37.0 40.0 27.0 40.0 40-41 35.557373406683695 37.0 37.0 40.0 27.0 40.0 42-43 35.46770044145318 37.0 37.0 40.0 27.0 40.0 44-45 35.34057182440266 37.0 37.0 40.0 27.0 40.0 46-47 35.23823156175692 37.0 37.0 40.0 27.0 40.0 48-49 35.10599857593007 37.0 37.0 40.0 27.0 40.0 50-51 34.026461275451744 37.0 35.0 38.5 24.5 38.5 52-53 34.49689939787163 37.0 35.0 38.5 27.0 40.0 54-55 35.21124560660408 37.0 37.0 40.0 27.0 40.0 56-57 35.33362119909917 37.0 37.0 40.0 27.0 40.0 58-59 35.05584918028887 37.0 37.0 40.0 27.0 40.0 60-61 34.831297632738654 37.0 33.0 40.0 27.0 40.0 62-63 34.522998708055894 37.0 33.0 40.0 27.0 40.0 64-65 34.29201951553583 37.0 33.0 40.0 27.0 40.0 66-67 34.07628970306528 37.0 33.0 37.0 27.0 40.0 68-69 33.95452768843117 37.0 33.0 37.0 27.0 40.0 70-71 33.57229533986712 37.0 33.0 37.0 22.0 40.0 72-73 33.42105418560576 37.0 33.0 37.0 24.5 38.5 74-75 33.26359154088869 37.0 33.0 37.0 27.0 37.0 76-77 32.976672889183824 37.0 33.0 37.0 22.0 37.0 78-79 32.6550721657631 37.0 33.0 37.0 22.0 37.0 80-81 32.38206889065718 37.0 33.0 37.0 22.0 37.0 82-83 32.125584423088114 37.0 33.0 37.0 22.0 37.0 84-85 31.944509492454173 33.0 33.0 37.0 22.0 37.0 86-87 31.79212721294816 33.0 33.0 37.0 22.0 37.0 88-89 31.648165617802913 33.0 33.0 37.0 15.0 37.0 90-91 31.491757218169468 33.0 33.0 37.0 15.0 37.0 92-93 31.332434190933277 33.0 33.0 37.0 15.0 37.0 94-95 31.20258658595804 33.0 33.0 37.0 15.0 37.0 96-97 30.995254303966703 33.0 33.0 37.0 15.0 37.0 98-99 30.877273381915415 33.0 33.0 37.0 15.0 37.0 100-101 29.645025186749862 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.25056516015882124 2306 2 0.1448332262580081 2306 3 0.1934751797728005 2306 4 0.2509309911760056 2306 5 0.20342126716624165 2306 6 0.22750757462249993 2306 7 0.39252563928548767 2306 8 0.2106863729822166 2306 9 0.26931055308247664 2306 10-11 0.3121210955187408 2306 12-13 0.29405556946305467 2306 14-15 0.564577250264314 2306 16-17 0.5507317635429914 2306 18-19 0.5315827908606465 2306 20-21 0.5061902635929982 2306 22-23 0.47466003929964273 2306 24-25 0.5695499456390323 2306 26-27 0.6255321757207568 2306 28-29 0.5258751771946848 2306 30-31 0.6224725291440549 2306 32-33 0.6131281594151261 2306 34-35 0.4714904515745175 2306 36-37 0.592238707004185 2306 38-39 0.6300742639514283 2306 40-41 0.6454651755262191 2306 42-43 0.5508117080497144 2306 44-45 0.6075382274530412 2306 46-47 0.6879895052693712 2306 48-49 0.581619905724672 2306 50-51 0.342090750586344 2306 52-53 0.47058036268680326 2306 54-55 0.5853694396377236 2306 56-57 0.6400491671357145 2306 58-59 0.6344960682600842 2306 60-61 0.5187447161702181 2306 62-63 0.7156898422020674 2306 64-65 0.6714489333632727 2306 66-67 0.6896684782777456 2306 68-69 0.5752305471041694 2306 70-71 0.594482614502553 2306 72-73 0.6408691318226261 2306 74-75 0.54953061907257 2306 76-77 0.6898247841379828 2306 78-79 0.5604908592620887 2306 80-81 0.608233996905355 2306 82-83 0.7205124143980868 2306 84-85 0.6942470791451711 2306 86-87 0.5665082345665695 2306 88-89 0.6474233406199126 2306 90-91 0.6074185691100986 2306 92-93 0.5762096451280776 2306 94-95 0.5109118896361906 2306 96-97 0.6539467599792026 2306 98-99 0.6019692962634053 2306 100-101 0.5764444883825703 2307 1 0.08296740215996223 2307 2 0.023030829331961655 2307 3 0.050569057938165685 2307 4 0.1778030324075104 2307 5 0.10176474028350668 2307 6 0.18149506942158666 2307 7 0.32833282584537216 2307 8 0.18349314538640726 2307 9 0.17813039152341048 2307 10-11 0.17031597350579375 2307 12-13 0.13656837521445198 2307 14-15 0.27675204937004594 2307 16-17 0.3085261831785715 2307 18-19 0.34777238356920037 2307 20-21 0.22004792969148212 2307 22-23 0.24894824516186276 2307 24-25 0.4472889336035877 2307 26-27 0.2989382994727805 2307 28-29 0.28711646027201 2307 30-31 0.37111951271012344 2307 32-33 0.3540394433361129 2307 34-35 0.2212382552071972 2307 36-37 0.16983280039629278 2307 38-39 0.15040552139329577 2307 40-41 0.25173931064068 2307 42-43 0.2503775276465632 2307 44-45 0.25539660154683475 2307 46-47 0.04859879347763041 2307 48-49 0.2540096942943606 2307 50-51 0.16156986112948601 2307 52-53 0.19265565178089616 2307 54-55 0.2650459595986874 2307 56-57 0.2583281249589362 2307 58-59 0.33324412211182164 2307 60-61 0.26974798915058784 2307 62-63 0.32720575507981664 2307 64-65 0.24694768178861892 2307 66-67 0.24824520397438476 2307 68-69 0.1723755811665555 2307 70-71 0.18608633893179416 2307 72-73 0.22963416414018667 2307 74-75 0.3579465238972901 2307 76-77 0.32951029424932443 2307 78-79 0.3654149618768301 2307 80-81 0.3685266525444888 2307 82-83 0.3016899251690077 2307 84-85 0.26183264975009024 2307 86-87 0.3222080881740368 2307 88-89 0.42633388826410723 2307 90-91 0.32552264921471163 2307 92-93 0.2872079472814981 2307 94-95 0.2977629977902616 2307 96-97 0.28938783339021157 2307 98-99 0.19899904740568886 2307 100-101 0.2790503856911357 2201 1 0.14102685613726607 2201 2 0.022743950622164277 2201 3 0.06398341503913585 2201 4 0.09650536464939563 2201 5 0.11398122562372492 2201 6 0.16159069150503313 2201 7 0.2833877678425054 2201 8 0.2109438973554134 2201 9 0.14921887351453478 2201 10-11 0.25617281158118743 2201 12-13 0.21111556785240992 2201 14-15 0.39297546091545854 2201 16-17 0.40204407411025755 2201 18-19 0.4838464935910807 2201 20-21 0.37561276407223687 2201 22-23 0.4539972170084994 2201 24-25 0.46506544605555433 2201 26-27 0.44205703133390983 2201 28-29 0.5075183230420279 2201 30-31 0.5089788534726125 2201 32-33 0.4848508916551708 2201 34-35 0.44378630920808604 2201 36-37 0.39399300268783577 2201 38-39 0.4818585168929417 2201 40-41 0.43303964679649454 2201 42-43 0.4443102869317599 2201 44-45 0.4441796128450548 2201 46-47 0.39147647661900464 2201 48-49 0.3064775572338263 2201 50-51 0.3503384921521757 2201 52-53 0.49749060151949465 2201 54-55 0.6558983704181358 2201 56-57 0.6672031741737499 2201 58-59 0.5888569461196838 2201 60-61 0.4684495489069107 2201 62-63 0.6676834896371702 2201 64-65 0.6671459260867607 2201 66-67 0.5674936025204502 2201 68-69 0.5936050002408066 2201 70-71 0.6346546345462372 2201 72-73 0.6371826459153382 2201 74-75 0.6418007554568987 2201 76-77 0.6707520523267831 2201 78-79 0.7010604047104039 2201 80-81 0.7081458794608722 2201 82-83 0.6969924861662946 2201 84-85 0.7235365223946317 2201 86-87 0.7065690245760479 2201 88-89 0.8438259412150906 2201 90-91 0.897525623284114 2201 92-93 0.726969336459721 2201 94-95 0.7023065211218231 2201 96-97 0.6771840463005248 2201 98-99 0.6504424117547316 2201 100-101 0.6558990557700284 1101 1 0.04997577485210414 1101 2 -0.026759658196823466 1101 3 -0.07502688647808853 1101 4 -0.0072317074340162435 1101 5 0.035169244752843554 1101 6 0.08664281133188467 1101 7 0.18788247649527534 1101 8 0.056864764106890675 1101 9 0.14252068778790772 1101 10-11 0.10520272747745452 1101 12-13 0.14883422282754566 1101 14-15 0.33521544567746986 1101 16-17 0.28968411284015616 1101 18-19 0.21774218137552026 1101 20-21 0.280595814074168 1101 22-23 0.30987534028657393 1101 24-25 0.35429994105899 1101 26-27 0.25447481533991123 1101 28-29 0.3099622973460612 1101 30-31 0.1990851339287545 1101 32-33 0.21999015374635178 1101 34-35 0.2527260669875133 1101 36-37 0.2714026801730256 1101 38-39 0.29361544371656834 1101 40-41 0.2801605507359213 1101 42-43 0.29736838404220833 1101 44-45 0.3121802386911412 1101 46-47 0.13847778953524426 1101 48-49 0.22578386580769205 1101 50-51 0.09292047005255455 1101 52-53 0.2013764254254582 1101 54-55 0.33602077321358337 1101 56-57 0.33891860468101953 1101 58-59 0.3340294279395337 1101 60-61 0.14686184995701979 1101 62-63 0.20741033956789323 1101 64-65 0.34995955774005694 1101 66-67 0.2988488087208623 1101 68-69 0.3457514935753139 1101 70-71 0.3523400471171243 1101 72-73 0.3343803974045656 1101 74-75 0.2952547228902276 1101 76-77 0.3877108485254439 1101 78-79 0.40061632609869946 1101 80-81 0.5033187014144644 1101 82-83 0.3649979192359112 1101 84-85 0.3874711895057388 1101 86-87 0.3387901659288275 1101 88-89 0.4378058914648584 1101 90-91 0.35057900387336005 1101 92-93 0.40907462826045915 1101 94-95 0.3862200999410099 1101 96-97 0.4521272553036404 1101 98-99 0.5087099065336815 1101 100-101 0.36332621612947946 2304 1 0.10928978203318351 2304 2 0.10676220904693778 2304 3 0.040962386733060185 2304 4 0.1211475774738986 2304 5 0.11100658229212712 2304 6 0.21508249555999726 2304 7 0.30888155135049544 2304 8 0.29355119708279886 2304 9 0.23448651856384117 2304 10-11 0.27486547756202384 2304 12-13 0.32800619786901564 2304 14-15 0.4210837979823836 2304 16-17 0.4757956111263937 2304 18-19 0.48075411859608863 2304 20-21 0.41564816422719986 2304 22-23 0.35800129039396467 2304 24-25 0.3401217100825349 2304 26-27 0.4399560740258792 2304 28-29 0.4972560247014073 2304 30-31 0.42379888924222087 2304 32-33 0.4268163365663753 2304 34-35 0.38618313334118426 2304 36-37 0.366341282348543 2304 38-39 0.4767427183704527 2304 40-41 0.607142011160505 2304 42-43 0.5179827290845296 2304 44-45 0.4471441091382715 2304 46-47 0.4373077870491713 2304 48-49 0.43729148500668913 2304 50-51 0.31836309273612784 2304 52-53 0.46539057565755826 2304 54-55 0.5168640406456362 2304 56-57 0.5888624439096048 2304 58-59 0.5490514154113555 2304 60-61 0.5156021858620079 2304 62-63 0.6133258049727743 2304 64-65 0.5844774839485538 2304 66-67 0.4416562767177794 2304 68-69 0.42080522940776177 2304 70-71 0.41774867989985864 2304 72-73 0.3656429551242226 2304 74-75 0.528223133475187 2304 76-77 0.6078098430927739 2304 78-79 0.67997993245163 2304 80-81 0.6718146686798292 2304 82-83 0.6175885284050366 2304 84-85 0.5558708928073273 2304 86-87 0.6679119304692875 2304 88-89 0.565164460465585 2304 90-91 0.47650119389891543 2304 92-93 0.5128315719407404 2304 94-95 0.5732810739106178 2304 96-97 0.7514761082463721 2304 98-99 0.7282695457679722 2304 100-101 0.7725448559628738 2202 1 0.09261485749419762 2202 2 0.04351377018330993 2202 3 0.03476455467914619 2202 4 0.029728724463581102 2202 5 -0.003130718007945177 2202 6 0.20659681223102666 2202 7 0.15655878676538748 2202 8 0.18301791564343972 2202 9 0.09035963690912041 2202 10-11 0.2069966152311551 2202 12-13 0.22159310765807305 2202 14-15 0.2176753556473514 2202 16-17 0.28311044771128735 2202 18-19 0.37394540324206815 2202 20-21 0.3592618401493226 2202 22-23 0.28979657840331896 2202 24-25 0.29043555350884986 2202 26-27 0.2519736914105337 2202 28-29 0.24329080694818117 2202 30-31 0.24242523978071517 2202 32-33 0.21098455628865054 2202 34-35 0.2666434576962402 2202 36-37 0.370433689046763 2202 38-39 0.3013214893953773 2202 40-41 0.3073104009108292 2202 42-43 0.3810306765494076 2202 44-45 0.3902385132231885 2202 46-47 0.18746907015870562 2202 48-49 0.23093777177580677 2202 50-51 0.3701853372745276 2202 52-53 0.25366891333236197 2202 54-55 0.3345019924984669 2202 56-57 0.2830981169439042 2202 58-59 0.3293550394439322 2202 60-61 0.19984760451399808 2202 62-63 0.35939623111883634 2202 64-65 0.35753277715615894 2202 66-67 0.32172606791537106 2202 68-69 0.24696915769312255 2202 70-71 0.38578695037816146 2202 72-73 0.36966097455435687 2202 74-75 0.38729224766008485 2202 76-77 0.24393112710557574 2202 78-79 0.4201103359892784 2202 80-81 0.32095040374780126 2202 82-83 0.328253050238807 2202 84-85 0.32166534661659085 2202 86-87 0.27749898814732177 2202 88-89 0.12538327858472442 2202 90-91 0.23007776031545646 2202 92-93 0.3123484753404604 2202 94-95 0.2975317882276407 2202 96-97 0.4081377980505785 2202 98-99 0.3570300324909468 2202 100-101 0.3148062333405548 1102 1 0.04910858439191301 1102 2 0.048409311683855094 1102 3 0.048228831045165066 1102 4 0.0013042920512447154 1102 5 -0.018596706761698556 1102 6 0.07661728890861497 1102 7 0.112289848686558 1102 8 0.1600768003959132 1102 9 0.09052164577241939 1102 10-11 0.17998246253038985 1102 12-13 0.17098647087404117 1102 14-15 0.25226084985400377 1102 16-17 0.27799684474032205 1102 18-19 0.2649431150529651 1102 20-21 0.19269601639477685 1102 22-23 0.20777756867294528 1102 24-25 0.17867710814149973 1102 26-27 0.2132779423729474 1102 28-29 0.29272626140019753 1102 30-31 0.2449739505319144 1102 32-33 0.3069561667434897 1102 34-35 0.24150467247662277 1102 36-37 0.2060375736866149 1102 38-39 0.1935790903489618 1102 40-41 0.2306027964231987 1102 42-43 0.31146026476599076 1102 44-45 0.23356888728955738 1102 46-47 0.17672564300651317 1102 48-49 0.19610110881404808 1102 50-51 0.20237881559899762 1102 52-53 0.3752598015344617 1102 54-55 0.44315379007839795 1102 56-57 0.40999047496706 1102 58-59 0.41543702175409436 1102 60-61 0.5131453347329256 1102 62-63 0.5436204384404704 1102 64-65 0.44537381455140235 1102 66-67 0.43512078338431337 1102 68-69 0.42488537548585015 1102 70-71 0.4366040433423848 1102 72-73 0.4883624673660236 1102 74-75 0.4180576854930891 1102 76-77 0.45966016828495526 1102 78-79 0.45936104467287464 1102 80-81 0.4763626972107744 1102 82-83 0.4437458549222626 1102 84-85 0.467795367176123 1102 86-87 0.4211426613355087 1102 88-89 0.37740042809346974 1102 90-91 0.3073617487134719 1102 92-93 0.49181199752059257 1102 94-95 0.3996857876523592 1102 96-97 0.5243226841446891 1102 98-99 0.5049245451089988 1102 100-101 0.47037383653884035 2305 1 0.05376952282499303 2305 2 -0.03334779278693745 2305 3 0.048498029010207944 2305 4 0.022843157731777808 2305 5 0.05375091933848353 2305 6 0.14958833262950577 2305 7 0.08270959112692111 2305 8 0.17714563386691395 2305 9 0.11885888172847103 2305 10-11 0.08847282053228156 2305 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-1.2777059514282172 1213 58-59 -0.9328927211885443 1213 60-61 -0.5961133113813588 1213 62-63 -0.5556986016775483 1213 64-65 -0.4580391736573759 1213 66-67 -0.364431105552363 1213 68-69 -0.3459341620855838 1213 70-71 -0.39736091827020914 1213 72-73 -0.33868774673560154 1213 74-75 -0.3734485634631639 1213 76-77 -0.4021056365804867 1213 78-79 -0.4294743501529652 1213 80-81 -0.45634454226775034 1213 82-83 -0.42043477515100136 1213 84-85 -0.45707348308427953 1213 86-87 -0.5077032531953556 1213 88-89 -0.452045960742943 1213 90-91 -0.47674688763228446 1213 92-93 -0.44726084023350765 1213 94-95 -0.3119185141106833 1213 96-97 -0.30849647429180393 1213 98-99 -0.45395396664450516 1213 100-101 -0.27407161090808785 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1782.0 3 365.0 4 441.0 5 616.0 6 1883.0 7 4709.0 8 13413.0 9 30038.0 10 41679.0 11 38115.0 12 27436.0 13 20032.0 14 15735.0 15 13937.0 16 13342.0 17 13974.0 18 14672.0 19 16082.0 20 18132.0 21 19920.0 22 22162.0 23 24329.0 24 27546.0 25 31244.0 26 36847.0 27 43793.0 28 53041.0 29 64797.0 30 79713.0 31 101690.0 32 139126.0 33 197485.0 34 298745.0 35 495547.0 36 932275.0 37 1454782.0 38 397850.0 39 359.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.96672573290901 24.08880632958452 26.304918936160337 14.639549001346131 2 29.409803416843545 23.225687495994606 34.805913228840716 12.558595858321139 3 38.4170153342792 19.37954017385307 26.81671348090373 15.386731010964006 4 28.166968343513155 18.464985619975717 36.56906583479915 16.79898020171198 5 33.114844544382336 17.485971244536806 33.657540215269 15.74164399581186 6 25.402659753677987 18.417254282048575 40.7448237664331 15.435262197840343 7 32.64508317457698 18.998643119621306 31.81151929987439 16.54475440592732 8 27.679262683001877 28.053415677044473 27.992899683577477 16.274421956376177 9 31.867525314636307 25.364602203432213 25.15315078559145 17.61472169634003 10-11 25.35886830452091 28.738258006791007 28.791625630319356 17.111248058368727 12-13 26.442930172158807 25.15576328955737 28.77655673449634 19.62474980378748 14-15 24.314188970438995 25.687942784339672 30.233694547928174 19.76417369729316 16-17 23.721465842571586 26.893405502958746 31.3914588409244 17.993669813545264 18-19 22.952046364372965 27.27391595808939 31.468081121069076 18.305956556468576 20-21 22.667805051704075 28.243337030037484 30.583390087822675 18.505467830435762 22-23 23.863363629168088 27.227931119884865 30.2450213580103 18.66368389293675 24-25 22.385477046559355 28.578101729128157 30.181620294303357 18.854800930009127 26-27 22.17078914237652 28.844690529161497 29.864135793848856 19.120384534613123 28-29 22.057978237677958 28.607242885194182 29.622354698481967 19.71242417864589 30-31 22.52472272700927 28.100398734414583 29.802009355512897 19.57286918306325 32-33 22.45918416941799 27.73805219787716 30.5599231902644 19.24284044244045 34-35 21.467615613764636 27.948830088724552 30.153346048764078 20.430208248746734 36-37 20.56459000214752 28.464565649374425 30.38805104810179 20.58279330037627 38-39 21.06041390236319 28.766680014550865 30.49522924165081 19.67767684143513 40-41 21.655516460760822 27.95251650520745 30.67660452061647 19.71536251341526 42-43 22.059033293938572 28.12883984313451 30.830671539591737 18.98145532333518 44-45 21.06095738019425 28.990207703273423 30.95623442891592 18.99260048761641 46-47 21.87036689377316 28.97798027564713 29.96406998969423 19.18758284088548 48-49 21.065318649775875 28.786807183278796 30.24278012174662 19.9050940451987 50-51 20.80538622680029 28.393226195962395 30.308697665102063 20.492689912135244 52-53 22.5048303921044 27.987653212916946 29.43718656815218 20.070329826826473 54-55 22.980637167315386 28.212957800878257 29.18470707213025 19.621697959676105 56-57 23.152456702771364 28.234780246186688 28.779852030474736 19.832911020567217 58-59 22.40004680822175 28.113420840124455 29.139769968284018 20.346762383369782 60-61 20.999705915572196 28.433435694603975 28.99244117778682 21.574417212037 62-63 22.08324006674922 28.40414153707095 29.17132289322905 20.341295502950775 64-65 21.145511963969486 28.918389911387553 29.82194410429025 20.114154020352714 66-67 21.11356172526883 28.441422537298816 29.181330660401034 21.26368507703132 68-69 21.222047631372096 27.871192229143226 30.833073258436734 20.07368688104794 70-71 21.028422655495007 29.894621962563008 29.396778838865345 19.68017654307664 72-73 20.700696855500276 31.435068952645867 28.284752866422252 19.5794813254316 74-75 21.169932399824347 30.982312988365834 28.075177310102355 19.772577301707464 76-77 21.233900039993546 30.420281693501977 28.453907382442512 19.891910884061964 78-79 21.083012606239464 30.207000151818225 28.98618986866392 19.723797373278394 80-81 20.581350275655833 30.518566679175652 28.821532160983605 20.078550884184914 82-83 19.852022237697632 30.39996478597783 29.14403707448213 20.603975901842407 84-85 19.96790478725706 29.971118784256618 29.274781184988758 20.78619524349757 86-87 19.958731533394985 29.994124449759994 29.794496422287615 20.252647594557406 88-89 20.125941204488086 30.227455775152617 29.55478812221225 20.091814898147046 90-91 20.360174162463046 29.81690905210265 29.24554569409726 20.577371091337046 92-93 19.99747511873334 30.574767380664735 29.039767412625256 20.387990087976664 94-95 19.64146743307877 30.991877246221872 29.06442645069911 20.30222887000025 96-97 20.104640852827103 31.117436967134882 28.760881576864154 20.017040603173854 98-99 20.50256709956433 30.40530099825848 28.43417903007137 20.65795287210582 100-101 20.517458129137207 30.3315543971653 27.932217970623043 21.21876950307445 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1111.0 1 808.0 2 413.0 3 414.0 4 531.0 5 603.5 6 761.5 7 1020.5 8 1352.5 9 1711.5 10 1948.0 11 2004.0 12 1957.0 13 1808.5 14 1631.0 15 1510.0 16 1397.5 17 1312.0 18 1366.0 19 1514.5 20 1747.5 21 2175.5 22 2767.0 23 3625.0 24 4862.0 25 6555.0 26 9288.5 27 13046.0 28 17227.0 29 21801.5 30 27338.0 31 34095.0 32 46366.5 33 68047.0 34 99368.0 35 137591.0 36 188401.0 37 249574.5 38 305107.5 39 328845.0 40 323150.5 41 300636.5 42 278699.5 43 280111.5 44 275825.5 45 257653.0 46 243237.5 47 215940.5 48 175113.0 49 145680.0 50 123417.0 51 104872.0 52 89560.0 53 73133.0 54 56584.5 55 42257.5 56 32053.5 57 24012.5 58 18482.5 59 14316.5 60 10696.5 61 7888.5 62 5759.5 63 4238.0 64 3140.0 65 2215.5 66 1564.0 67 1116.0 68 839.5 69 653.0 70 478.5 71 353.0 72 286.0 73 229.5 74 174.0 75 135.5 76 108.0 77 91.5 78 73.5 79 66.0 80 68.0 81 58.5 82 42.5 83 31.5 84 27.0 85 23.0 86 12.5 87 10.0 88 12.0 89 9.5 90 6.5 91 9.0 92 8.5 93 6.0 94 5.5 95 5.0 96 4.0 97 2.0 98 1.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2381663485309181 2 0.23032801615418702 3 0.21218726859394763 4 0.20073778037969814 5 0.20403030481978848 6 0.13055390457287036 7 0.10482972975384237 8 0.10079373205308655 9 0.09401750433444911 10-11 0.07373130536486056 12-13 0.10249309950603636 14-15 0.10484035080042331 16-17 0.09463352503614342 18-19 0.08911058081405648 20-21 0.0827273318189137 22-23 0.16479615874980935 24-25 0.6382293100950499 26-27 0.36725454867561924 28-29 0.1825757907262969 30-31 0.31408558949145154 32-33 0.35181154694693767 34-35 0.3334371363619177 36-37 0.3436227200330357 38-39 0.35056888449696816 40-41 0.352926756837936 42-43 0.3717578724259363 44-45 0.34935808518674133 46-47 0.37266066138531584 48-49 0.3609137838668002 50-51 0.36749883274698075 52-53 0.36156166770823733 54-55 0.344068803989435 56-57 0.3526293675336698 58-59 0.343070425610827 60-61 0.3208405751169271 62-63 0.30897686608602115 64-65 0.3080315929403178 66-67 0.3220195112874111 68-69 0.3202351754618137 70-71 0.3056418574596071 72-73 0.29692197821665833 74-75 0.30382565849426696 76-77 0.3053975733882456 78-79 0.3084670558501362 80-81 0.31878009208022545 82-83 0.3466922024949263 84-85 0.33258745263544276 86-87 0.343240362356122 88-89 0.34493972980907184 90-91 0.32603426689500503 92-93 0.3046965843139038 94-95 0.3045372686151897 96-97 0.3127898218085773 98-99 0.3348391145106013 100-101 0.3412754687386488 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4707634.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.58973408690839 #Duplication Level Percentage of deduplicated Percentage of total 1 91.5590815215469 47.23508668938172 2 4.06840866545309 4.197762424151975 3 1.1552714035665508 1.7880043352462336 4 0.7046721307939945 1.4541539138446922 5 0.4470841360655022 1.1532475847047212 6 0.2932742807111135 0.9077965293849402 7 0.2050237349493451 0.7403983977279049 8 0.15780259938707977 0.6512795312481902 9 0.13556245652030705 0.6294267975645649 >10 1.0006704329809217 10.059657101300958 >50 0.12267631237034714 4.366204392657299 >100 0.12275226563745435 13.837549116933292 >500 0.021532141792014074 7.686310237525711 >1k 0.006021230163164461 4.641761992947386 >5k 1.6668806218008447E-4 0.6513609553804608 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT 8359 0.17756265674009492 Illumina Single End PCR Primer 1 (96% over 32bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 8172 0.1735903853188247 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 7039 0.14952309376642278 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 6728 0.14291680279308036 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.001423220241845479 0.0 0.0 2 0.001550672800816716 0.0 0.0 3 0.0017418516392735714 0.0 0.0 4 0.0018480621050829356 0.0 0.0 5 0.0021242093161872823 0.0 0.0 6 0.0023153881546441376 0.0 0.0 7 0.002740230017881594 0.0 0.0 8 0.0031225876947953046 0.0 0.0 9 0.0032925244400902874 0.0 0.0 10-11 0.005353007476791951 0.0 0.0 12-13 0.0075834272587885975 0.0 0.0 14-15 0.009718257621556816 0.0 1.0621046580936411E-5 16-17 0.011778740658258478 0.0 2.1242093161872823E-5 18-19 0.014688907421435056 0.0 2.1242093161872823E-5 20-21 0.018236336979467818 0.0 2.1242093161872823E-5 22-23 0.02183687177040526 0.0 2.1242093161872823E-5 24-25 0.026074669356198888 0.0 2.1242093161872823E-5 26-27 0.03080103508471559 0.0 2.1242093161872823E-5 28-29 0.036154042561507546 0.0 3.186313974280923E-5 30-31 0.055452484199069 0.0 4.2484186323745646E-5 32-33 0.06148523865704088 0.0 4.2484186323745646E-5 34-35 0.06750737206843183 0.0 4.2484186323745646E-5 36-37 0.0746447153708211 0.0 4.2484186323745646E-5 38-39 0.08149529041552508 0.0 4.2484186323745646E-5 40-41 0.08948231744438927 0.0 5.310523290468206E-5 42-43 0.0980110178488812 0.0 6.372627948561847E-5 44-45 0.10785672802940925 0.0 6.372627948561847E-5 46-47 0.11789361704839416 0.0 6.372627948561847E-5 48-49 0.12862087409513995 0.0 6.372627948561847E-5 50-51 0.1413661299922636 0.0 6.372627948561847E-5 52-53 0.15521597473380472 0.0 6.372627948561847E-5 54-55 0.16969246122362103 0.0 6.372627948561847E-5 56-57 0.18453006329718918 0.0 8.496837264749129E-5 58-59 0.20161932724591589 0.0 8.496837264749129E-5 60-61 0.22020615876255462 0.0 9.55894192284277E-5 62-63 0.24074726285008563 0.0 1.062104658093641E-4 64-65 0.2619999770585394 0.0 1.062104658093641E-4 66-67 0.2858973318656463 0.0 1.062104658093641E-4 68-69 0.3117914434299693 0.0 1.062104658093641E-4 70-71 0.34051075338482134 0.0 1.1683151239030051E-4 72-73 0.37252258777976366 0.0 1.2745255897123693E-4 74-75 0.4060744739289418 0.0 1.2745255897123693E-4 76-77 0.44476694662329314 0.0 1.2745255897123693E-4 78-79 0.4867731858508967 0.0 1.2745255897123693E-4 80-81 0.5325817597544753 0.0 1.2745255897123693E-4 82-83 0.582075836821639 0.0 1.2745255897123693E-4 84-85 0.6370503739245659 0.0 1.4869465213310974E-4 86-87 0.6983231916499881 0.0 1.4869465213310974E-4 88-89 0.7630690916073765 0.0 1.8055779187591898E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 1640 0.0 75.38771 9 CGGGAGA 2350 0.0 70.02759 4 AGAGCGG 1810 0.0 69.61715 8 GAGAGCG 2235 0.0 62.314278 7 TCGGGAG 2630 0.0 61.310574 3 AAGCGGC 700 0.0 59.550922 9 TCGGGAA 885 0.0 58.94699 3 CGGAAAG 965 0.0 55.039585 5 AAGCGTC 390 0.0 53.443142 9 GATCCGG 530 0.0 52.804016 1 GATCGGG 4150 0.0 50.977386 1 TTGTGTT 64580 0.0 49.799213 1 AGAGCGT 7640 0.0 48.611145 8 ATTCGGA 450 0.0 48.483047 2 GAGCGTC 7680 0.0 48.35693 9 ATCGGGA 4290 0.0 47.42907 2 AAGAGCG 8045 0.0 44.986794 7 TCGGAAG 8465 0.0 44.03599 3 CGGAAGA 8420 0.0 43.820274 4 CCGGATC 1250 0.0 42.929127 60-61 >>END_MODULE