FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n02_34344-1.34200000035854.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n02_34344-1.34200000035854.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4707634
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT83590.17756265674009492Illumina Single End PCR Primer 1 (96% over 32bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC81720.1735903853188247No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG70390.14952309376642278No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA67280.14291680279308036No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC16400.075.387719
CGGGAGA23500.070.027594
AGAGCGG18100.069.617158
GAGAGCG22350.062.3142787
TCGGGAG26300.061.3105743
AAGCGGC7000.059.5509229
TCGGGAA8850.058.946993
CGGAAAG9650.055.0395855
AAGCGTC3900.053.4431429
GATCCGG5300.052.8040161
GATCGGG41500.050.9773861
TTGTGTT645800.049.7992131
AGAGCGT76400.048.6111458
ATTCGGA4500.048.4830472
GAGCGTC76800.048.356939
ATCGGGA42900.047.429072
AAGAGCG80450.044.9867947
TCGGAAG84650.044.035993
CGGAAGA84200.043.8202744
CCGGATC12500.042.92912760-61
Produced by FastQC (version 0.11.2)