##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n02_34311-1.34200000035761.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2570498 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.909040582797573 33.0 33.0 33.0 27.0 33.0 2 31.884787305806114 33.0 33.0 33.0 27.0 33.0 3 31.858707923522992 33.0 33.0 33.0 27.0 33.0 4 35.56886720005229 37.0 37.0 37.0 33.0 37.0 5 35.578740384159026 37.0 37.0 37.0 33.0 37.0 6 35.56097806728501 37.0 37.0 37.0 33.0 37.0 7 35.483020410830896 37.0 37.0 37.0 33.0 37.0 8 35.464593631273004 37.0 37.0 37.0 33.0 37.0 9 35.43426954621245 37.0 37.0 37.0 33.0 37.0 10-11 35.32263845371597 37.0 37.0 37.0 33.0 37.0 12-13 35.31345754791484 37.0 37.0 37.0 33.0 37.0 14-15 36.92281164972702 40.0 37.0 40.0 33.0 40.0 16-17 36.87310318856502 40.0 37.0 40.0 33.0 40.0 18-19 36.87729945714799 40.0 37.0 40.0 33.0 40.0 20-21 36.880467325786675 40.0 37.0 40.0 33.0 40.0 22-23 36.85804443341329 40.0 37.0 40.0 33.0 40.0 24-25 36.555931185318954 40.0 37.0 40.0 33.0 40.0 26-27 36.46905774678681 40.0 37.0 40.0 33.0 40.0 28-29 36.4845973426161 40.0 37.0 40.0 33.0 40.0 30-31 36.47761678865341 40.0 37.0 40.0 33.0 40.0 32-33 36.37359997945923 38.5 37.0 40.0 33.0 40.0 34-35 36.31988957003662 37.0 37.0 40.0 33.0 40.0 36-37 36.233035777503034 37.0 37.0 40.0 33.0 40.0 38-39 36.15959164333137 37.0 37.0 40.0 33.0 40.0 40-41 36.00849251779228 37.0 37.0 40.0 30.0 40.0 42-43 35.938084371199665 37.0 37.0 40.0 27.0 40.0 44-45 35.853069716451834 37.0 37.0 40.0 27.0 40.0 46-47 35.76996091807891 37.0 37.0 40.0 27.0 40.0 48-49 35.63262060503451 37.0 37.0 40.0 27.0 40.0 50-51 34.4885067018142 37.0 35.0 38.5 27.0 38.5 52-53 34.99550943046833 37.0 35.0 38.5 27.0 40.0 54-55 35.788195711492484 37.0 37.0 40.0 27.0 40.0 56-57 35.86183650016456 37.0 37.0 40.0 27.0 40.0 58-59 35.62666592232323 37.0 37.0 40.0 27.0 40.0 60-61 35.36746848276093 37.0 37.0 40.0 27.0 40.0 62-63 35.1004490180502 37.0 35.0 40.0 27.0 40.0 64-65 34.85947003265515 37.0 33.0 38.5 27.0 40.0 66-67 34.63898688114132 37.0 33.0 37.0 27.0 40.0 68-69 34.436378670592234 37.0 33.0 37.0 27.0 40.0 70-71 34.06721168427285 37.0 33.0 37.0 27.0 40.0 72-73 33.779030561393164 37.0 33.0 37.0 27.0 38.5 74-75 33.55020447399687 37.0 33.0 37.0 27.0 37.0 76-77 33.28393817073579 37.0 33.0 37.0 27.0 37.0 78-79 33.03050070453274 37.0 33.0 37.0 27.0 37.0 80-81 32.85359062718586 37.0 33.0 37.0 27.0 37.0 82-83 32.67828763142395 37.0 33.0 37.0 27.0 37.0 84-85 32.51647307253303 35.0 33.0 37.0 22.0 37.0 86-87 32.398769810363596 33.0 33.0 37.0 22.0 37.0 88-89 32.27396053216147 33.0 33.0 37.0 22.0 37.0 90-91 32.13187036130742 33.0 33.0 37.0 22.0 37.0 92-93 31.987486276978238 33.0 33.0 37.0 22.0 37.0 94-95 31.871139561283456 33.0 33.0 37.0 22.0 37.0 96-97 31.669889064298047 33.0 33.0 37.0 22.0 37.0 98-99 31.54799225675336 33.0 33.0 37.0 22.0 37.0 100-101 30.317132516734112 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.3202216181240658 2306 2 0.299287352250186 2306 3 0.2192606612592769 2306 4 0.4068366133766119 2306 5 0.30628313131201423 2306 6 0.4219179073725954 2306 7 0.4470325451169259 2306 8 0.40966382778635335 2306 9 0.4088886200992903 2306 10-11 0.4682428774905887 2306 12-13 0.44378304267282687 2306 14-15 0.7173583133640022 2306 16-17 0.7084488693889028 2306 18-19 0.7156790834894409 2306 20-21 0.6161502381970507 2306 22-23 0.5999479760746382 2306 24-25 0.5972890119727694 2306 26-27 0.6188009243359573 2306 28-29 0.6147029138016151 2306 30-31 0.6202968740019656 2306 32-33 0.5495798247056314 2306 34-35 0.5771850699416206 2306 36-37 0.5365043951958484 2306 38-39 0.621235923438789 2306 40-41 0.7740273465708256 2306 42-43 0.6314620568848781 2306 44-45 0.657773991227458 2306 46-47 0.7054555186638183 2306 48-49 0.6899504263263836 2306 50-51 0.49428650672697927 2306 52-53 0.5095697803212644 2306 54-55 0.7240645526619431 2306 56-57 0.7692016086997953 2306 58-59 0.8094930207804154 2306 60-61 0.7069946922386876 2306 62-63 0.7086404685690937 2306 64-65 0.7142405221552934 2306 66-67 0.7153441487851637 2306 68-69 0.8560129082390162 2306 70-71 0.8641750863516009 2306 72-73 0.7773164834445794 2306 74-75 0.7406451076408942 2306 76-77 0.7178744853704089 2306 78-79 0.7522921136015484 2306 80-81 0.8103007784589806 2306 82-83 0.8794906297951854 2306 84-85 0.9032475667715687 2306 86-87 0.7602006349518504 2306 88-89 0.8557862848529183 2306 90-91 0.9559121892404008 2306 92-93 0.8934445671725157 2306 94-95 0.905069194934665 2306 96-97 0.9131381427733487 2306 98-99 0.8631812892217887 2306 100-101 0.7117897439566256 2307 1 0.2823140773577322 2307 2 0.287467126033075 2307 3 0.2614380548413635 2307 4 0.33536157509537645 2307 5 0.287340340438341 2307 6 0.41952751130639854 2307 7 0.5416370965157355 2307 8 0.5408434780497657 2307 9 0.47644514986846787 2307 10-11 0.4491014751327356 2307 12-13 0.5170691118319795 2307 14-15 0.7172291080648279 2307 16-17 0.7677946866859457 2307 18-19 0.7842961162377833 2307 20-21 0.8033344057443657 2307 22-23 0.7961037753143074 2307 24-25 0.7061102635976368 2307 26-27 0.8497794143004924 2307 28-29 0.7058271567546299 2307 30-31 0.6033666664304178 2307 32-33 0.5688627843108378 2307 34-35 0.640673887507262 2307 36-37 0.5466808319921057 2307 38-39 0.5612432953951583 2307 40-41 0.6717373345914055 2307 42-43 0.7959447161220794 2307 44-45 0.558235130626052 2307 46-47 0.4436871510227931 2307 48-49 0.4562145109784055 2307 50-51 0.43556573722021596 2307 52-53 0.4803262489188853 2307 54-55 0.5215149537081274 2307 56-57 0.503037319946543 2307 58-59 0.499955748286645 2307 60-61 0.49965626258303786 2307 62-63 0.6006440525946886 2307 64-65 0.6398194462025799 2307 66-67 0.5499037234075956 2307 68-69 0.44900758905880167 2307 70-71 0.46286715228355746 2307 72-73 0.4895872616761352 2307 74-75 0.5538283615361692 2307 76-77 0.651202394303219 2307 78-79 0.6049194514038021 2307 80-81 0.5582104563084087 2307 82-83 0.6660238692798544 2307 84-85 0.638472185968844 2307 86-87 0.48514235532334027 2307 88-89 0.5992176952689476 2307 90-91 0.5552618493657562 2307 92-93 0.6053551782802487 2307 94-95 0.6486586318622862 2307 96-97 0.6851525494582944 2307 98-99 0.6859569092209661 2307 100-101 0.44943652137255796 2201 1 0.08277936223144167 2201 2 0.023542796540141353 2201 3 0.016701648565483396 2201 4 0.07694035635523733 2201 5 0.13451941418083635 2201 6 0.20821912024211997 2201 7 0.22414875988572192 2201 8 0.4269486780535132 2201 9 0.3388723807516314 2201 10-11 0.3729383410688243 2201 12-13 0.3658894943717641 2201 14-15 0.5792157401085447 2201 16-17 0.5336587447574956 2201 18-19 0.6241439065252408 2201 20-21 0.540227683955969 2201 22-23 0.5527479120686891 2201 24-25 0.4648208401571523 2201 26-27 0.46978166416690925 2201 28-29 0.5712096809172635 2201 30-31 0.46335435466142627 2201 32-33 0.5628837643151527 2201 34-35 0.44703350990468493 2201 36-37 0.5029081184969755 2201 38-39 0.4217454843276869 2201 40-41 0.46722963348615565 2201 42-43 0.36474734971570655 2201 44-45 0.5031363708649152 2201 46-47 0.5062948399412619 2201 48-49 0.3972605113721315 2201 50-51 0.4757469452109859 2201 52-53 0.4918922809357369 2201 54-55 0.5215786999232037 2201 56-57 0.739342976798163 2201 58-59 0.6053715204751668 2201 60-61 0.6549475109151714 2201 62-63 0.6625877184395392 2201 64-65 0.5416563683853255 2201 66-67 0.5820286909876486 2201 68-69 0.7707278619175995 2201 70-71 0.7139225583688216 2201 72-73 0.7988768663558545 2201 74-75 0.7534572898185559 2201 76-77 0.7759488937298187 2201 78-79 0.7166414116624154 2201 80-81 0.8283642596763272 2201 82-83 0.6302141204836857 2201 84-85 0.6486300203474329 2201 86-87 0.6408987322884556 2201 88-89 0.6203656583586366 2201 90-91 0.6159735571081129 2201 92-93 0.7512772954045488 2201 94-95 0.7737593290083957 2201 96-97 0.787136337920856 2201 98-99 0.8220747647233004 2201 100-101 0.7564881339330611 1101 1 0.201085750675972 1101 2 0.2269582735816833 1101 3 0.20539975574448022 1101 4 0.22035470067490337 1101 5 0.23504794648317784 1101 6 0.2830108223063448 1101 7 0.32449028621776677 1101 8 0.31751492144552174 1101 9 0.38741806203575635 1101 10-11 0.4078080085935767 1101 12-13 0.37766632892704166 1101 14-15 0.5760596136326654 1101 16-17 0.5423131139115611 1101 18-19 0.4904851293733046 1101 20-21 0.5259380234626363 1101 22-23 0.5330561833961838 1101 24-25 0.6094710267891088 1101 26-27 0.637302413815064 1101 28-29 0.5926349549344394 1101 30-31 0.5458058212691057 1101 32-33 0.5780120130095838 1101 34-35 0.558676875627448 1101 36-37 0.4574605918379362 1101 38-39 0.46408230024648844 1101 40-41 0.603936073651191 1101 42-43 0.48581795355264745 1101 44-45 0.5780186372490448 1101 46-47 0.3490880038578723 1101 48-49 0.5106542529768987 1101 50-51 0.6595003473838901 1101 52-53 0.6433171595688592 1101 54-55 0.6684652108085558 1101 56-57 0.5977874718232741 1101 58-59 0.6958231024922483 1101 60-61 0.5940455437192256 1101 62-63 0.6677565634586031 1101 64-65 0.6564181922385259 1101 66-67 0.6099868850015326 1101 68-69 0.5743221700970267 1101 70-71 0.49172954469815267 1101 72-73 0.6435446315490267 1101 74-75 0.5128377764401435 1101 76-77 0.6319954379129129 1101 78-79 0.6670004096951132 1101 80-81 0.6421346313052823 1101 82-83 0.6954078746979988 1101 84-85 0.7139271621481882 1101 86-87 0.595606153433387 1101 88-89 0.6708206705995181 1101 90-91 0.6389456376510481 1101 92-93 0.5238318183309474 1101 94-95 0.4688303325037957 1101 96-97 0.4924122572154914 1101 98-99 0.7245177572890107 1101 100-101 0.7188777603378789 2304 1 0.17060941684736264 2304 2 0.14017477753271024 2304 3 0.1121657557017599 2304 4 0.15963725162693265 2304 5 0.15438236972068609 2304 6 0.20930294742575484 2304 7 0.2522938213025938 2304 8 0.24682589236932273 2304 9 0.1507983248348026 2304 10-11 0.22653555788392765 2304 12-13 0.33542658080718013 2304 14-15 0.3012112638141602 2304 16-17 0.44214103423628615 2304 18-19 0.3390120448898344 2304 20-21 0.4028503855294048 2304 22-23 0.3678780754678499 2304 24-25 0.34625270964289 2304 26-27 0.457314847863195 2304 28-29 0.3892520791472478 2304 30-31 0.3369719342902826 2304 32-33 0.5179373789148514 2304 34-35 0.46700781206576636 2304 36-37 0.4804753790656946 2304 38-39 0.5180238636976426 2304 40-41 0.41398872585899227 2304 42-43 0.39936132940416513 2304 44-45 0.4320055057417207 2304 46-47 0.4543540443015601 2304 48-49 0.5095299477659765 2304 50-51 0.48349274487999594 2304 52-53 0.47503848786101344 2304 54-55 0.6121323409577144 2304 56-57 0.5549049087501601 2304 58-59 0.44523865752123015 2304 60-61 0.47617009681628275 2304 62-63 0.6204814729354524 2304 64-65 0.5743950787190926 2304 66-67 0.457099384076308 2304 68-69 0.46440689847293726 2304 70-71 0.6378872350550822 2304 72-73 0.6579821361751499 2304 74-75 0.7603892177812952 2304 76-77 0.7356233931106004 2304 78-79 0.7194182682271375 2304 80-81 0.6981403808727222 2304 82-83 0.7335285649441516 2304 84-85 0.6454946917467268 2304 86-87 0.5938159336592292 2304 88-89 0.5778733044269799 2304 90-91 0.6163332307263332 2304 92-93 0.5730918608242916 2304 94-95 0.5946666998343026 2304 96-97 0.6968041885465652 2304 98-99 0.5870540894981566 2304 100-101 0.6739646670318429 2202 1 0.08234974368304648 2202 2 0.10484500264380259 2202 3 0.10885805554387673 2202 4 0.12364113423797818 2202 5 0.22941147314138277 2202 6 0.20950797588730552 2202 7 0.23943821352019512 2202 8 0.2926409797857943 2202 9 0.2705675015762665 2202 10-11 0.2728687577640514 2202 12-13 0.32468833057733093 2202 14-15 0.2658941877601464 2202 16-17 0.4608046273231281 2202 18-19 0.4198247749407713 2202 20-21 0.4408559203743181 2202 22-23 0.433475869657137 2202 24-25 0.455887754363566 2202 26-27 0.4425726549914444 2202 28-29 0.2956962991686538 2202 30-31 0.4592901135267198 2202 32-33 0.4357005322525964 2202 34-35 0.34392654829618863 2202 36-37 0.43914875256427877 2202 38-39 0.31655176857921674 2202 40-41 0.5014811585078363 2202 42-43 0.3721462254561132 2202 44-45 0.4478750088201551 2202 46-47 0.3219236673955308 2202 48-49 0.4425425547074795 2202 50-51 0.48059244643428656 2202 52-53 0.41590070642597254 2202 54-55 0.5599709530812689 2202 56-57 0.6365148947445007 2202 58-59 0.57636382508813 2202 60-61 0.47840359098613305 2202 62-63 0.4050465470758695 2202 64-65 0.6372805172190965 2202 66-67 0.5446613139852019 2202 68-69 0.3745110381545729 2202 70-71 0.5195097351739904 2202 72-73 0.4738796228679334 2202 74-75 0.535327536076899 2202 76-77 0.48911245571955675 2202 78-79 0.6235813157258647 2202 80-81 0.5183338834099516 2202 82-83 0.7601670263440852 2202 84-85 0.612544793960808 2202 86-87 0.6372504513089936 2202 88-89 0.6171157057905177 2202 90-91 0.6050108340913809 2202 92-93 0.4980976214775765 2202 94-95 0.6482305008587979 2202 96-97 0.6984456861224686 2202 98-99 0.6688104584256358 2202 100-101 0.4967424979138393 1102 1 0.03550480928960553 1102 2 0.04785012434518521 1102 3 0.0607616700017104 1102 4 0.09475025086643285 1102 5 0.11060380522274471 1102 6 0.12814804222553278 1102 7 0.16344835936673974 1102 8 0.12421251288174062 1102 9 0.15602655235427676 1102 10-11 0.13505206589535845 1102 12-13 0.2485971155146487 1102 14-15 0.27605310683726714 1102 16-17 0.27589657405744106 1102 18-19 0.4084256236798609 1102 20-21 0.27725921777443574 1102 22-23 0.3700672554431961 1102 24-25 0.2557821985370268 1102 26-27 0.42415228525337767 1102 28-29 0.31351903952278093 1102 30-31 0.40587886529493034 1102 32-33 0.4562213848939294 1102 34-35 0.35739262312181097 1102 36-37 0.3369806632027945 1102 38-39 0.3669054098650051 1102 40-41 0.4530827389185461 1102 42-43 0.4370574980769675 1102 44-45 0.4456637020546239 1102 46-47 0.47024170066219284 1102 48-49 0.4133315893887186 1102 50-51 0.38424212205986663 1102 52-53 0.5836019892902229 1102 54-55 0.48313226228570016 1102 56-57 0.44195706829700754 1102 58-59 0.5732630982943121 1102 60-61 0.6125499253112849 1102 62-63 0.7354919887616376 1102 64-65 0.6090819903179678 1102 66-67 0.40582515965126476 1102 68-69 0.4368983363351546 1102 70-71 0.55631935421696 1102 72-73 0.5478269425108735 1102 74-75 0.49173844286193713 1102 76-77 0.46003836177291646 1102 78-79 0.514063536105354 1102 80-81 0.5934634868439304 1102 82-83 0.6310016197785515 1102 84-85 0.674169592751106 1102 86-87 0.6750561193251841 1102 88-89 0.5218778149559213 1102 90-91 0.478371255534249 1102 92-93 0.4910528919521866 1102 94-95 0.5462090384911029 1102 96-97 0.6278605439342826 1102 98-99 0.6018838694787583 1102 100-101 0.8056679161861204 2305 1 0.04496843540596274 2305 2 1.7527825136554043E-4 2305 3 -0.009142778103726101 2305 4 -0.035364550139377116 2305 5 0.03239848136945511 2305 6 0.13384300450768194 2305 7 -0.04869124004364522 2305 8 0.04306849137822866 2305 9 -0.07182118890992228 2305 10-11 0.10038328934111718 2305 12-13 0.15603386224143634 2305 14-15 0.05509203859830336 2305 16-17 0.0988849552245199 2305 18-19 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-0.5750427014623014 1213 82-83 -0.39746541492828413 1213 84-85 -0.3301455634365098 1213 86-87 -0.4676762071750815 1213 88-89 -0.3562330101056901 1213 90-91 -0.3660488609353223 1213 92-93 -0.36982946827745167 1213 94-95 -0.21796756571887244 1213 96-97 -0.23229623566839308 1213 98-99 -0.3824320576658131 1213 100-101 -0.14783181535565504 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 971.0 3 187.0 4 220.0 5 309.0 6 953.0 7 1828.0 8 4743.0 9 8784.0 10 10652.0 11 10403.0 12 7833.0 13 6397.0 14 6042.0 15 6568.0 16 6806.0 17 7116.0 18 7402.0 19 7915.0 20 9047.0 21 10267.0 22 11350.0 23 12836.0 24 14440.0 25 16628.0 26 19796.0 27 23466.0 28 28576.0 29 35423.0 30 43517.0 31 56127.0 32 77674.0 33 111834.0 34 171984.0 35 290662.0 36 544347.0 37 811142.0 38 186136.0 39 117.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.68298378115319 23.160834557779836 28.109392206592982 13.046789454473986 2 32.64118895303723 20.385238377846804 35.498658239950835 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28.25599504744254 31.386190463212078 18.732269883233336 22-23 21.755360955341317 27.3617876722284 32.06802278934507 18.814828583085212 24-25 22.078183927315123 27.439738883175117 31.690325844011902 18.791751345497854 26-27 21.811662887325525 27.846497902734107 31.753201051512598 18.588638158427766 28-29 20.992070095878297 28.431670198300218 31.16479211429164 19.411467591529846 30-31 20.807737364482172 28.709330994022427 30.640433188765375 19.842498452730023 32-33 19.976577459317973 28.87351242468189 31.910986537894743 19.23892357810539 34-35 20.248423059224123 29.143867816825463 32.12168155495474 18.486027568995674 36-37 20.304111906498928 30.155594407641654 31.45147282064974 18.088820865209673 38-39 20.77184099809987 29.856607073322355 30.31683463993668 19.05471728864109 40-41 21.247305649046037 29.029170917737463 29.878988190822998 19.8445352423935 42-43 20.89199279443062 29.25293660922398 31.01510713569636 18.839963460649038 44-45 20.145199347547614 29.514628748385608 31.858815924225897 18.481355979840885 46-47 20.473008958748522 29.233032105305902 31.512897724078044 18.78106121186753 48-49 20.817346780419673 30.195313300649353 29.777806841772897 19.209533077158074 50-51 20.466161476845663 29.777463083446122 29.86759606913202 19.8887793705762 52-53 20.494033115651796 29.447678152500966 30.07524829320989 19.98304043863735 54-55 21.49108434318111 29.259760712479654 29.794284400484266 19.45487054385497 56-57 21.82991456494879 29.33894702324138 28.998553635527863 19.832584776281966 58-59 21.12436159067169 29.213228325310443 30.130090294616828 19.53231978940104 60-61 20.130346422937066 29.58176945269137 30.30521161292966 19.982672511441898 62-63 20.90183245077341 29.635439439141802 29.73418235461564 19.72854575546915 64-65 20.68720291766243 29.73458254486952 29.641986212784655 19.9362283246834 66-67 21.11534675992379 29.33470393517272 29.42424966183756 20.125699643065932 68-69 21.9687401033515 29.121781534295405 29.4605237508832 19.448954611469897 70-71 21.126856464135738 30.694333779979882 29.153949466248267 19.02486028963611 72-73 20.300187902746284 31.29458593507367 28.893383139005895 19.51184302317415 74-75 21.227355235043568 30.190464187441762 28.70717062763087 19.8750099498838 76-77 21.545935604680878 29.341303229403813 29.13444049023523 19.978320675680077 78-79 21.475721267954267 29.29625927698488 29.68443959977781 19.543579855283035 80-81 19.811810957095606 30.075241665630887 29.986109140903338 20.12683823637017 82-83 19.16982581562255 29.765907111164115 30.281162467924627 20.78310460528871 84-85 19.107080757171914 29.094969659808136 30.777135903061264 21.020813679958692 86-87 19.1462265973779 29.171602899323496 31.86234576021366 19.81982474308495 88-89 19.525061431295512 29.782360484111788 31.262313095649358 19.430264988943343 90-91 19.881579234938553 29.53277597037666 30.308113323009287 20.27753147167549 92-93 19.086842746514744 30.593501294563225 30.43270270971563 19.886953249206396 94-95 18.773294615627137 30.882768383626814 30.786721991053618 19.557215009692435 96-97 19.477444550846048 31.630738565014372 29.742981591481836 19.148835292657743 98-99 20.13825477448707 30.5125149219492 29.022866758922383 20.32636354464134 100-101 19.609206623244745 30.524335980505217 28.646562056179427 21.219895340070607 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1288.0 1 884.5 2 427.0 3 493.0 4 580.0 5 560.0 6 695.5 7 899.5 8 1124.5 9 1318.5 10 1398.5 11 1402.5 12 1337.5 13 1230.5 14 1112.5 15 988.5 16 869.0 17 865.0 18 942.5 19 983.5 20 1076.5 21 1228.0 22 1515.5 23 1913.5 24 2398.5 25 3131.5 26 4321.5 27 6268.0 28 8420.0 29 10439.0 30 12838.5 31 16245.0 32 23267.0 33 34422.0 34 50625.0 35 77119.5 36 121569.0 37 179581.0 38 227671.0 39 234362.0 40 211290.0 41 183718.5 42 159389.0 43 148799.0 44 133225.5 45 114752.5 46 110501.5 47 99076.0 48 75193.0 49 57784.0 50 46978.5 51 39611.0 52 34044.0 53 28086.5 54 22573.0 55 16717.5 56 11765.0 57 8932.0 58 7283.0 59 5710.0 60 4342.0 61 3369.5 62 2575.5 63 1952.5 64 1460.5 65 1030.5 66 746.0 67 561.0 68 400.5 69 294.5 70 244.5 71 200.5 72 142.0 73 109.0 74 87.0 75 70.5 76 63.5 77 44.5 78 29.5 79 29.5 80 26.0 81 16.5 82 14.0 83 12.5 84 12.5 85 10.0 86 10.0 87 9.5 88 8.5 89 7.0 90 5.0 91 4.5 92 2.0 93 2.0 94 2.0 95 1.5 96 1.0 97 0.5 98 0.0 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23629662423390332 2 0.23170607407591837 3 0.21085408352778334 4 0.19894977549097492 5 0.20427948203033033 6 0.13032494092584396 7 0.10503801208948617 8 0.09994172335477404 9 0.09340602482476158 10-11 0.07356551143008086 12-13 0.10128387573147306 14-15 0.1035013448755844 16-17 0.09400902082009012 18-19 0.08780399751332232 20-21 0.08278551471349131 22-23 0.16382039589215786 24-25 0.6317452882670984 26-27 0.36667991961090807 28-29 0.18054867189159454 30-31 0.3081115021291594 32-33 0.34479700042559847 34-35 0.327446276947113 36-37 0.3363161535235584 38-39 0.3435326539837806 40-41 0.34578902609533246 42-43 0.366602113676027 44-45 0.34150969967687195 46-47 0.36669937109462836 48-49 0.3540364551927292 50-51 0.3611167952669094 52-53 0.35444493635085494 54-55 0.33785282073746026 56-57 0.3463531191232205 58-59 0.33697750397004783 60-61 0.31493897291497597 62-63 0.3027623441060837 64-65 0.30194538178983216 66-67 0.3158920956172695 68-69 0.3142387195010461 70-71 0.2993972374224761 72-73 0.29060516678091175 74-75 0.297763312789973 76-77 0.29906656219923144 78-79 0.3023344114642377 80-81 0.31206015332437526 82-83 0.34073164032806097 84-85 0.3262013819890153 86-87 0.33686079506772615 88-89 0.3381834959607049 90-91 0.31877091520787026 92-93 0.2981717939480988 94-95 0.298541372138784 96-97 0.30655538343153743 98-99 0.3274268254633927 100-101 0.3338458150910835 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2570498.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.953608494487675 #Duplication Level Percentage of deduplicated Percentage of total 1 86.89243725655919 36.45451293793519 2 5.778870765213969 4.848889632480546 3 1.971522646655763 2.4813746776743613 4 1.1251394619564994 1.8881464195448596 5 0.771548456700508 1.6184620943469643 6 0.5354135276619676 1.3477517713309641 7 0.42027741783668526 1.2342507972896155 8 0.2550705751394867 0.8560904838292662 9 0.20191653989016828 0.762401471680235 >10 1.654825995093432 13.8881073385865 >50 0.17746396371373602 5.187292160283242 >100 0.18493804034973085 17.25927619717526 >500 0.024060945053413142 6.671267333735875 >1k 0.006232923155125363 4.839438075484089 >5k 2.8148502037669517E-4 0.6627386086230717 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 5850 0.22758235952722003 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 5531 0.21517231291368444 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5455 0.21221568738820257 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 4991 0.1941647104957872 No Hit TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT 4976 0.19358116598417893 No Hit GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT 4556 0.17724191965914776 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 3910 0.1521106026925522 No Hit TGTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAG 3907 0.15199389379023054 No Hit GTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGT 3816 0.14845372375314045 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3685 0.14335743501842835 No Hit TTGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGG 3123 0.121493967316839 No Hit TGTGTTTGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAA 2786 0.1083836672893735 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 2778 0.10807244354984909 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 2775 0.10795573464752743 No Hit GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT 2698 0.10496020615460505 No Hit TTGTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTA 2608 0.10145893908495551 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.334178046433026E-4 0.0 0.0 2 2.72320772083853E-4 0.0 0.0 3 3.112237395244034E-4 0.0 0.0 4 3.112237395244034E-4 0.0 0.0 5 3.890296744055043E-4 0.0 0.0 6 4.668356092866052E-4 0.0 0.0 7 6.224474790488068E-4 0.0 0.0 8 7.391563813704581E-4 0.0 0.0 9 8.947682511326599E-4 0.0 0.0 10-11 0.001536667213901742 0.0 0.0 12-13 0.0024119839813141267 0.0 0.0 14-15 0.0034234611347684378 0.0 0.0 16-17 0.004396035320782199 0.0 0.0 18-19 0.005854896599802839 0.0 0.0 20-21 0.0071192430416207285 0.0 0.0 22-23 0.008383589483438618 0.0 0.0 24-25 0.009706290376417332 0.0 0.0 26-27 0.011048442753116321 0.0 0.0 28-29 0.012779624804220817 0.0 0.0 30-31 0.020307349003967322 0.0 0.0 32-33 0.022291400343435398 0.0 0.0 34-35 0.02415874278058182 0.0 0.0 36-37 0.026026085217728236 0.0 0.0 38-39 0.027932330622315208 0.0 0.0 40-41 0.030577732408272638 0.0 0.0 42-43 0.03324258567795034 0.0 1.9451483720275214E-5 44-45 0.036490983459236306 0.0 3.890296744055043E-5 46-47 0.039700478273081714 0.0 3.890296744055043E-5 48-49 0.0433184542450529 3.890296744055043E-5 3.890296744055043E-5 50-51 0.04720875098910794 3.890296744055043E-5 3.890296744055043E-5 52-53 0.051021241798281894 3.890296744055043E-5 3.890296744055043E-5 54-55 0.05498934447721804 3.890296744055043E-5 3.890296744055043E-5 56-57 0.06029959953285317 3.890296744055043E-5 3.890296744055043E-5 58-59 0.06592107832801271 3.890296744055043E-5 3.890296744055043E-5 60-61 0.07202884421617911 3.890296744055043E-5 3.890296744055043E-5 62-63 0.08015956441125416 3.890296744055043E-5 3.890296744055043E-5 64-65 0.08865986279701443 3.890296744055043E-5 3.890296744055043E-5 66-67 0.09813273536878846 3.890296744055043E-5 3.890296744055043E-5 68-69 0.10892830883354121 3.890296744055043E-5 3.890296744055043E-5 70-71 0.12159122473544037 3.890296744055043E-5 7.780593488110086E-5 72-73 0.13581025933496155 3.890296744055043E-5 7.780593488110086E-5 74-75 0.15251908385067797 3.890296744055043E-5 7.780593488110086E-5 76-77 0.1718733101523518 3.890296744055043E-5 7.780593488110086E-5 78-79 0.19474825500739545 3.890296744055043E-5 7.780593488110086E-5 80-81 0.22026860164839654 3.890296744055043E-5 1.167089023216513E-4 82-83 0.249562536131131 3.890296744055043E-5 1.167089023216513E-4 84-85 0.28449740089274533 3.890296744055043E-5 1.167089023216513E-4 86-87 0.32559838599368685 3.890296744055043E-5 1.167089023216513E-4 88-89 0.37486899425714393 3.890296744055043E-5 1.167089023216513E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 410 0.0 56.616604 9 CGCGTCT 55 8.2614497E-7 51.75733 2 TCGGGAA 220 0.0 49.595943 3 AGAGCGG 460 0.0 49.433743 8 TTGTGTT 34555 0.0 48.7228 1 CGGGAGA 545 0.0 43.52002 4 CGCGATA 90 1.21108314E-8 42.16853 3 ATCGGGA 785 0.0 40.493793 2 GGCCGCA 60 8.3425926E-5 39.543793 1 TGTGTTT 60125 0.0 38.838085 1 GGCGCCG 410 0.0 38.227444 56-57 GGGCGCC 570 0.0 36.24985 54-55 AACCGAG 120 3.8344297E-9 35.589417 1 CCGTGTA 215 0.0 35.365265 38-39 AGAGCGT 1720 0.0 34.70421 8 GCCGTAT 435 0.0 34.387344 58-59 CGCGTTA 35 2.058261E-4 33.917503 60-61 CCCGTGT 330 0.0 33.841476 38-39 TCTCGGG 445 0.0 33.627068 48-49 TCGGGTA 180 0.0 32.89765 14-15 >>END_MODULE