FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n02_34311-1.34200000035761.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n02_34311-1.34200000035761.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2570498
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG58500.22758235952722003No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG55310.21517231291368444No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC54550.21221568738820257No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC49910.1941647104957872No Hit
TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT49760.19358116598417893No Hit
GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT45560.17724191965914776No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA39100.1521106026925522No Hit
TGTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAG39070.15199389379023054No Hit
GTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGT38160.14845372375314045No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA36850.14335743501842835No Hit
TTGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGG31230.121493967316839No Hit
TGTGTTTGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAA27860.1083836672893735No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA27780.10807244354984909No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA27750.10795573464752743No Hit
GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT26980.10496020615460505No Hit
TTGTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTA26080.10145893908495551No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC4100.056.6166049
CGCGTCT558.2614497E-751.757332
TCGGGAA2200.049.5959433
AGAGCGG4600.049.4337438
TTGTGTT345550.048.72281
CGGGAGA5450.043.520024
CGCGATA901.21108314E-842.168533
ATCGGGA7850.040.4937932
GGCCGCA608.3425926E-539.5437931
TGTGTTT601250.038.8380851
GGCGCCG4100.038.22744456-57
GGGCGCC5700.036.2498554-55
AACCGAG1203.8344297E-935.5894171
CCGTGTA2150.035.36526538-39
AGAGCGT17200.034.704218
GCCGTAT4350.034.38734458-59
CGCGTTA352.058261E-433.91750360-61
CCCGTGT3300.033.84147638-39
TCTCGGG4450.033.62706848-49
TCGGGTA1800.032.8976514-15
Produced by FastQC (version 0.11.2)