##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n02_34310-1.34200000035810.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5656279 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.944502737577125 33.0 33.0 33.0 27.0 33.0 2 31.9230566596874 33.0 33.0 33.0 33.0 33.0 3 31.881646043273324 33.0 33.0 33.0 33.0 33.0 4 35.59804351942328 37.0 37.0 37.0 33.0 37.0 5 35.59014963724385 37.0 37.0 37.0 33.0 37.0 6 35.569214849550384 37.0 37.0 37.0 33.0 37.0 7 35.49838082598118 37.0 37.0 37.0 33.0 37.0 8 35.48511167854344 37.0 37.0 37.0 33.0 37.0 9 35.43416581819956 37.0 37.0 37.0 33.0 37.0 10-11 35.31699046316492 37.0 37.0 37.0 33.0 37.0 12-13 35.30546645595099 37.0 37.0 37.0 33.0 37.0 14-15 36.94262526654007 40.0 37.0 40.0 33.0 40.0 16-17 36.84778774172915 40.0 37.0 40.0 33.0 40.0 18-19 36.87587900101816 40.0 37.0 40.0 33.0 40.0 20-21 36.88153236429815 40.0 37.0 40.0 33.0 40.0 22-23 36.86886458394291 40.0 37.0 40.0 33.0 40.0 24-25 36.561016703737565 40.0 37.0 40.0 33.0 40.0 26-27 36.48037048738225 40.0 37.0 40.0 33.0 40.0 28-29 36.51078199996853 40.0 37.0 40.0 33.0 40.0 30-31 36.517342231527124 40.0 37.0 40.0 33.0 40.0 32-33 36.44747129694274 40.0 37.0 40.0 33.0 40.0 34-35 36.37322867206515 40.0 37.0 40.0 33.0 40.0 36-37 36.262130987527314 37.0 37.0 40.0 33.0 40.0 38-39 36.137616885588564 37.0 37.0 40.0 33.0 40.0 40-41 36.02708565118517 37.0 37.0 40.0 33.0 40.0 42-43 35.92337409452398 37.0 37.0 40.0 27.0 40.0 44-45 35.80035885782861 37.0 37.0 40.0 27.0 40.0 46-47 35.67727458281319 37.0 37.0 40.0 27.0 40.0 48-49 35.52367934820754 37.0 37.0 40.0 27.0 40.0 50-51 34.33063529928421 37.0 35.0 38.5 27.0 38.5 52-53 34.81696818703604 37.0 35.0 38.5 27.0 40.0 54-55 35.57916953884347 37.0 37.0 40.0 27.0 40.0 56-57 35.6028361578345 37.0 37.0 40.0 27.0 40.0 58-59 35.394887610741975 37.0 37.0 40.0 27.0 40.0 60-61 35.13321257667806 37.0 33.0 40.0 27.0 40.0 62-63 34.8714085708997 37.0 33.0 40.0 27.0 40.0 64-65 34.611329197163016 37.0 33.0 37.0 27.0 40.0 66-67 34.33627646373172 37.0 33.0 37.0 27.0 40.0 68-69 34.120541967608034 37.0 33.0 37.0 27.0 40.0 70-71 33.790424146333656 37.0 33.0 37.0 27.0 37.0 72-73 33.496251776123486 37.0 33.0 37.0 27.0 37.0 74-75 33.26857232466786 37.0 33.0 37.0 27.0 37.0 76-77 33.00765759609807 37.0 33.0 37.0 27.0 37.0 78-79 32.81094169859726 37.0 33.0 37.0 27.0 37.0 80-81 32.67108455930126 37.0 33.0 37.0 27.0 37.0 82-83 32.425665176700086 33.0 33.0 37.0 22.0 37.0 84-85 32.26016370479603 33.0 33.0 37.0 22.0 37.0 86-87 32.1057209165248 33.0 33.0 37.0 22.0 37.0 88-89 31.99225683527987 33.0 33.0 37.0 22.0 37.0 90-91 31.84758257151035 33.0 33.0 37.0 22.0 37.0 92-93 31.66118803545582 33.0 33.0 37.0 22.0 37.0 94-95 31.503026194429236 33.0 33.0 37.0 22.0 37.0 96-97 31.286268941118358 33.0 33.0 37.0 15.0 37.0 98-99 31.162159433790308 33.0 33.0 37.0 15.0 37.0 100-101 29.822679538968995 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.2608192293494831 2306 2 0.2589778402116636 2306 3 0.2538118385911474 2306 4 0.36365128963381466 2306 5 0.3425986410845212 2306 6 0.35366670348182794 2306 7 0.46045323979983266 2306 8 0.23196605066120668 2306 9 0.3518498542510784 2306 10-11 0.4358510824968391 2306 12-13 0.4297675276488704 2306 14-15 0.6432598435149757 2306 16-17 0.7082259393169394 2306 18-19 0.6584285619708723 2306 20-21 0.6614746420358841 2306 22-23 0.6377422472932892 2306 24-25 0.6977503133008369 2306 26-27 0.622358218130735 2306 28-29 0.6120793732873935 2306 30-31 0.602202927031577 2306 32-33 0.5662041457932077 2306 34-35 0.6880238995423227 2306 36-37 0.6914970592135745 2306 38-39 0.6821723170599583 2306 40-41 0.7873281044467006 2306 42-43 0.6920228510464668 2306 44-45 0.7597331504654505 2306 46-47 0.7846616186612039 2306 48-49 0.7637467320273785 2306 50-51 0.5848281930160368 2306 52-53 0.6287543858955971 2306 54-55 0.7908742480749424 2306 56-57 0.7042168472754327 2306 58-59 0.7712693498383132 2306 60-61 0.7297146576482874 2306 62-63 0.6900370249881362 2306 64-65 0.6738954343883492 2306 66-67 0.7119648539090164 2306 68-69 0.635856843056807 2306 70-71 0.7100912955857694 2306 72-73 0.7238922779848878 2306 74-75 0.5741114967394481 2306 76-77 0.5945123738460296 2306 78-79 0.5572060086823072 2306 80-81 0.5754466248059487 2306 82-83 0.6463219012359644 2306 84-85 0.6316285253959606 2306 86-87 0.678381067359652 2306 88-89 0.6491738077226685 2306 90-91 0.717604113491138 2306 92-93 0.6357372001154182 2306 94-95 0.683959089784917 2306 96-97 0.8200616832323284 2306 98-99 0.726042632193991 2306 100-101 0.6283610047509001 2307 1 0.2960147778898481 2307 2 0.2713762558243147 2307 3 0.24632221932078835 2307 4 0.3707879007308179 2307 5 0.33195770333782804 2307 6 0.37335993999239747 2307 7 0.4297194529317423 2307 8 0.36937310240317345 2307 9 0.4528502552945284 2307 10-11 0.4149828213860758 2307 12-13 0.42889747594656313 2307 14-15 0.6828120893378511 2307 16-17 0.6678201986313965 2307 18-19 0.7250445974131381 2307 20-21 0.6595163358373739 2307 22-23 0.6488447324953555 2307 24-25 0.6664868097672709 2307 26-27 0.6889654641396206 2307 28-29 0.5400834891950836 2307 30-31 0.624150653205156 2307 32-33 0.5788188223481896 2307 34-35 0.4479120120994011 2307 36-37 0.473557743914526 2307 38-39 0.43514927770659284 2307 40-41 0.5011729746267761 2307 42-43 0.5333010145372796 2307 44-45 0.47219787519879475 2307 46-47 0.39700161520266164 2307 48-49 0.46225823126351173 2307 50-51 0.35893280526098437 2307 52-53 0.32449189907463705 2307 54-55 0.36497980058486235 2307 56-57 0.3253878737054592 2307 58-59 0.29810492079786144 2307 60-61 0.3700154770278843 2307 62-63 0.41501412135998095 2307 64-65 0.35026226282147377 2307 66-67 0.36870569521722985 2307 68-69 0.35970224877910084 2307 70-71 0.3231234089048627 2307 72-73 0.3868851659024415 2307 74-75 0.41216802628686366 2307 76-77 0.37120618964158325 2307 78-79 0.3841788583269903 2307 80-81 0.43443886925521724 2307 82-83 0.35854379232139877 2307 84-85 0.380132379869103 2307 86-87 0.3273843456139005 2307 88-89 0.42045905590560295 2307 90-91 0.3757077257847392 2307 92-93 0.388414093594438 2307 94-95 0.4176886221584475 2307 96-97 0.3678209091735063 2307 98-99 0.2751196599264958 2307 100-101 0.23624107926893956 2201 1 0.18853295256377223 2201 2 0.15379426007628183 2201 3 0.1999560718023261 2201 4 0.28792306558937497 2201 5 0.2960307875399337 2201 6 0.35921656059777973 2201 7 0.3925179043335305 2201 8 0.34081804135242777 2201 9 0.36823020797995554 2201 10-11 0.38400018572654915 2201 12-13 0.4871036052071318 2201 14-15 0.6248091421966748 2201 16-17 0.6532733350787652 2201 18-19 0.6680292148107796 2201 20-21 0.5542261307131682 2201 22-23 0.6325627567017378 2201 24-25 0.5968121988677026 2201 26-27 0.6388755843978444 2201 28-29 0.6504130777564399 2201 30-31 0.554796428554134 2201 32-33 0.5043395394361596 2201 34-35 0.5480117962873763 2201 36-37 0.44482821060221056 2201 38-39 0.5599894711194793 2201 40-41 0.6726330658325637 2201 42-43 0.6486949674469358 2201 44-45 0.6003999717399466 2201 46-47 0.6097488163250162 2201 48-49 0.5789441884080304 2201 50-51 0.5960789896117404 2201 52-53 0.5635039513334874 2201 54-55 0.617606237191886 2201 56-57 0.6502227859597056 2201 58-59 0.7544109425374401 2201 60-61 0.7355984087112191 2201 62-63 0.7359304563474467 2201 64-65 0.7167822818384977 2201 66-67 0.7356951370836526 2201 68-69 0.6598997071169066 2201 70-71 0.6789577522370536 2201 72-73 0.7440882145679808 2201 74-75 0.5950372694677313 2201 76-77 0.5774861542293266 2201 78-79 0.6681918695011575 2201 80-81 0.6977477982999076 2201 82-83 0.776179212051936 2201 84-85 0.7949974044289192 2201 86-87 0.6205303354530969 2201 88-89 0.5885879850558027 2201 90-91 0.6948375011936143 2201 92-93 0.738337541047482 2201 94-95 0.6731106773804463 2201 96-97 0.6638353078796406 2201 98-99 0.6507334581933897 2201 100-101 0.5216150650333411 1101 1 0.2975759723940321 1101 2 0.250488153803893 1101 3 0.23898174367468528 1101 4 0.3481363293662483 1101 5 0.31301050473170733 1101 6 0.40294473356588156 1101 7 0.4088546708176821 1101 8 0.40175453856354437 1101 9 0.441358498346311 1101 10-11 0.44349098445171364 1101 12-13 0.465549017907783 1101 14-15 0.5906941500943148 1101 16-17 0.6675306994556749 1101 18-19 0.6121602533899946 1101 20-21 0.5870458683301365 1101 22-23 0.5430790996947721 1101 24-25 0.6404974219018627 1101 26-27 0.6942112883414353 1101 28-29 0.7094826968702819 1101 30-31 0.6336704980230152 1101 32-33 0.5885457862377663 1101 34-35 0.6055368075362253 1101 36-37 0.602406563496352 1101 38-39 0.6819311165465507 1101 40-41 0.5449410310077738 1101 42-43 0.6129045569775116 1101 44-45 0.6918671685093116 1101 46-47 0.5542111867512105 1101 48-49 0.5916666159414774 1101 50-51 0.57226837820658 1101 52-53 0.5949263190761727 1101 54-55 0.7072576135584256 1101 56-57 0.7115180383254156 1101 58-59 0.6741063037696975 1101 60-61 0.7141532496827949 1101 62-63 0.7157401984735046 1101 64-65 0.7398889488605604 1101 66-67 0.5813106059276123 1101 68-69 0.6699887552335042 1101 70-71 0.6291482024715833 1101 72-73 0.7289820524527215 1101 74-75 0.5785602334275666 1101 76-77 0.6342184861606128 1101 78-79 0.7507128944344288 1101 80-81 0.7410242418270556 1101 82-83 0.657590470043047 1101 84-85 0.6795113380922757 1101 86-87 0.6400453705530751 1101 88-89 0.6121956639846715 1101 90-91 0.6886470405726648 1101 92-93 0.6084638562467894 1101 94-95 0.6019582694471204 1101 96-97 0.6063836639146167 1101 98-99 0.6474211200737052 1101 100-101 0.6093783665430941 2304 1 0.14271653667331563 2304 2 0.09863145456716893 2304 3 0.14135404967032628 2304 4 0.2010868264364376 2304 5 0.0984310741413168 2304 6 0.3168990699521359 2304 7 0.3312608388132716 2304 8 0.24518075132775152 2304 9 0.2744359240853598 2304 10-11 0.3010509778609318 2304 12-13 0.2900014636578945 2304 14-15 0.3565900513599516 2304 16-17 0.4161047179304447 2304 18-19 0.4711221214264967 2304 20-21 0.4449394497164292 2304 22-23 0.47154253538177926 2304 24-25 0.4535931080309865 2304 26-27 0.45982534954394083 2304 28-29 0.48236515163591065 2304 30-31 0.35858792604636847 2304 32-33 0.3267243017958421 2304 34-35 0.33906346414821087 2304 36-37 0.3104101706372404 2304 38-39 0.39441149165924827 2304 40-41 0.4324511535644362 2304 42-43 0.41093324465416003 2304 44-45 0.4066435033059079 2304 46-47 0.31266259707495436 2304 48-49 0.4869093185728275 2304 50-51 0.2903429813321452 2304 52-53 0.3571429928110774 2304 54-55 0.42061157116211234 2304 56-57 0.43360985705277955 2304 58-59 0.3751437663572048 2304 60-61 0.3953249432228034 2304 62-63 0.4540619745386749 2304 64-65 0.48913320931242055 2304 66-67 0.4032554427917887 2304 68-69 0.4506329289941533 2304 70-71 0.40803187053973033 2304 72-73 0.40245737307446916 2304 74-75 0.4243741057068817 2304 76-77 0.48814215295553964 2304 78-79 0.45527092371658284 2304 80-81 0.5761710926236105 2304 82-83 0.49330563510742564 2304 84-85 0.4694042987844611 2304 86-87 0.4317336632136275 2304 88-89 0.4140198774210617 2304 90-91 0.4576091754233751 2304 92-93 0.47579474986843096 2304 94-95 0.3692395683923131 2304 96-97 0.4142771516818833 2304 98-99 0.34355250126321124 2304 100-101 0.3483247219077903 2202 1 0.12671872012014873 2202 2 0.1292119157694529 2202 3 0.08221925742834202 2202 4 0.13959135183341687 2202 5 0.147063029428125 2202 6 0.21613359214514105 2202 7 0.23920405902438802 2202 8 0.2209329640171731 2202 9 0.2215567578636879 2202 10-11 0.31652416537120587 2202 12-13 0.37295108333469074 2202 14-15 0.45457655060642566 2202 16-17 0.47604605474916184 2202 18-19 0.4476141292991116 2202 20-21 0.45835968016500317 2202 22-23 0.4697993802672116 2202 24-25 0.33726891872886 2202 26-27 0.4378851841241058 2202 28-29 0.4247802167409418 2202 30-31 0.4059549485402769 2202 32-33 0.42647090838381274 2202 34-35 0.4423928815171223 2202 36-37 0.35039373545323116 2202 38-39 0.4263257116957604 2202 40-41 0.48852873882141523 2202 42-43 0.4742071350037662 2202 44-45 0.48927598919741655 2202 46-47 0.3756979272110996 2202 48-49 0.4101159848665432 2202 50-51 0.399768843310504 2202 52-53 0.4274367579515328 2202 54-55 0.5322107933939435 2202 56-57 0.4879945081674677 2202 58-59 0.48034661670463663 2202 60-61 0.4880998617249688 2202 62-63 0.546751496197686 2202 64-65 0.5195247379455807 2202 66-67 0.5361084771556506 2202 68-69 0.5112062872133549 2202 70-71 0.5836833022400612 2202 72-73 0.6346425744584536 2202 74-75 0.6161387138646361 2202 76-77 0.5594868714044807 2202 78-79 0.643225773155379 2202 80-81 0.5612613971364624 2202 82-83 0.5955458295155651 2202 84-85 0.6906441253292783 2202 86-87 0.5026324109526215 2202 88-89 0.43596283995832863 2202 90-91 0.5258299798743487 2202 92-93 0.5883620290190557 2202 94-95 0.6175816829791962 2202 96-97 0.6350648199539286 2202 98-99 0.5809739488816774 2202 100-101 0.5422062394033382 1102 1 -0.01786801950659722 1102 2 -0.019118475801008117 1102 3 0.007075878309798611 1102 4 -0.017248051167172207 1102 5 -0.11374771165522901 1102 6 -0.006804361520174496 1102 7 0.0929344846239033 1102 8 0.11985439953260624 1102 9 -0.04181558526924789 1102 10-11 0.11491299830914414 1102 12-13 0.09627989997849085 1102 14-15 0.2058440530949639 1102 16-17 0.102372384262722 1102 18-19 0.17906362111644825 1102 20-21 0.12123625766587054 1102 22-23 0.11472825323601654 1102 24-25 0.12555500565584765 1102 26-27 0.19535126734725594 1102 28-29 0.16754203243340982 1102 30-31 0.23268793969340607 1102 32-33 0.2402184108237222 1102 34-35 0.23334950450354341 1102 36-37 0.10231721673949323 1102 38-39 0.19016732217738053 1102 40-41 0.26861211760408565 1102 42-43 0.3149765791925816 1102 44-45 0.31126324570406894 1102 46-47 0.1806899773461268 1102 48-49 0.20289443132850238 1102 50-51 0.06549934368578647 1102 52-53 0.25531444910982515 1102 54-55 0.3724439456113373 1102 56-57 0.33826862519595835 1102 58-59 0.4131160756358341 1102 60-61 0.32366308095255647 1102 62-63 0.3689411184303495 1102 64-65 0.322043963259496 1102 66-67 0.34331040205418617 1102 68-69 0.34420110313995167 1102 70-71 0.2870703368359315 1102 72-73 0.3745841125732312 1102 74-75 0.3158452645640111 1102 76-77 0.37777570998859034 1102 78-79 0.3840896407511991 1102 80-81 0.31118738128323997 1102 82-83 0.3083705503241987 1102 84-85 0.373943834896842 1102 86-87 0.35992631232748096 1102 88-89 0.25196028375168567 1102 90-91 0.17687454570107164 1102 92-93 0.3359562478185829 1102 94-95 0.3072118377673192 1102 96-97 0.37323571495985775 1102 98-99 0.23309846983990568 1102 100-101 0.30321032220595256 2305 1 0.008135361647365613 2305 2 0.03144541096840214 2305 3 0.01354129585068975 2305 4 0.002447434166619189 2305 5 -0.02483519125499356 2305 6 0.020346175956817092 2305 7 -0.026305358884997077 2305 8 0.09422110658495342 2305 9 -0.0034095645431335697 2305 10-11 0.049766347929477206 2305 12-13 0.01677769203928392 2305 14-15 0.010418448729801355 2305 16-17 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-0.6620290954266252 1213 60-61 -0.6175727666235815 1213 62-63 -0.5258431259737861 1213 64-65 -0.46060460368168776 1213 66-67 -0.3247170521386664 1213 68-69 -0.42067091909520826 1213 70-71 -0.472770859421054 1213 72-73 -0.5085151010120512 1213 74-75 -0.37187696254511593 1213 76-77 -0.31338928119696163 1213 78-79 -0.40995810653393505 1213 80-81 -0.3916172014188888 1213 82-83 -0.31640980042672595 1213 84-85 -0.33333690283897965 1213 86-87 -0.41836854654193445 1213 88-89 -0.2905745739217416 1213 90-91 -0.3303165993012662 1213 92-93 -0.28387326569901106 1213 94-95 -0.18951853468990976 1213 96-97 -0.22322348108061973 1213 98-99 -0.271011011715121 1213 100-101 -0.11025747319745349 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2171.0 3 418.0 4 483.0 5 741.0 6 2084.0 7 4771.0 8 14290.0 9 22845.0 10 23588.0 11 24389.0 12 19751.0 13 15375.0 14 13151.0 15 12400.0 16 12728.0 17 13546.0 18 14855.0 19 16903.0 20 18707.0 21 21077.0 22 24134.0 23 27172.0 24 31825.0 25 36955.0 26 44207.0 27 53347.0 28 64700.0 29 80351.0 30 101037.0 31 134612.0 32 185608.0 33 268166.0 34 407779.0 35 685275.0 36 1260216.0 37 1696749.0 38 299753.0 39 120.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.08011972238442 23.850095382731517 24.875568287036014 15.194216607848055 2 32.0185993500753 21.664103505975007 33.49957995351403 12.817717190435662 3 40.61232332040446 18.742666493517817 24.751469093018315 15.893541093059419 4 31.428587624685722 17.572128572549648 34.23382041713042 16.765463385634213 5 34.02685868023035 16.276150471958744 32.880474497327576 16.816516350483333 6 26.557519072240137 17.122814321988415 39.307950649383756 17.011715956387697 7 34.382675901419276 17.274172867560587 29.743460412944582 18.59969081807555 8 29.943583811018954 25.828063616380533 25.982593608595632 18.24575896400488 9 32.048454115589756 24.795449047670363 24.238613196126657 18.91748364061322 10-11 26.85697487999877 27.742605685166982 27.955145047543585 17.445274387290667 12-13 27.35030656894003 25.113717339641184 27.718458298598353 19.817517792820436 14-15 26.358696157141026 25.67933591126771 27.818356592939303 20.143611338651965 16-17 25.032178214974632 27.045662495185546 28.96087127289471 18.96128801694511 18-19 24.090381498170824 27.256354564776693 29.08863721279565 19.56462672425684 20-21 23.774549333733454 28.122031539892788 28.673193603692543 19.430225522681212 22-23 24.348377036352197 27.492660481738728 28.428846383028382 19.730116098880696 24-25 24.30152383881888 27.184834784254708 28.90905722029931 19.6045841566271 26-27 23.217803318631255 27.190469448031546 29.306816363132455 20.284910870204747 28-29 22.551788243312465 27.815541822030802 28.365934710574585 21.266735224082144 30-31 21.722333420944448 28.684224714350528 28.51518314801986 21.078258716685163 32-33 21.480684281475 27.562587001547445 29.573482620271047 21.38324609670651 34-35 21.75709710604442 27.8158734252637 29.283831292694636 21.14319817599725 36-37 21.280155106021134 29.169669563255173 29.484478088515914 20.06569724220778 38-39 21.85851308946596 28.485531481621774 28.01016379197013 21.645791636942143 40-41 22.298173424639185 27.79792126755397 27.56128836524588 22.342616942560966 42-43 23.21714621660906 28.045939052560055 27.999463732658487 20.737450998172395 44-45 21.69546673857814 28.942723930908215 28.03163631718637 21.33017301332728 46-47 22.07121625334979 28.66706138482545 28.62976043009314 20.63196193173162 48-49 22.383735501398483 28.109993884773083 28.668818987597707 20.83745162623073 50-51 21.32047723376608 28.520186366539196 27.687305664866376 22.47203073482835 52-53 21.824253434804064 28.635237497540327 27.890344613319833 21.650164454335776 54-55 22.337229176540212 28.55104631432973 27.96450996029648 21.147214548833578 56-57 22.767365482176313 28.32851215714376 27.117911844196808 21.786210516483116 58-59 21.971425887609012 27.686062095044033 27.88182379655117 22.46068822079578 60-61 20.8886390049258 27.685194094363023 28.66825199122588 22.7579149094853 62-63 21.412287756490002 27.807191695491912 28.545336694529837 22.235183853488248 64-65 21.741194137869606 28.499319411694344 28.233911696693216 21.525574753742838 66-67 22.058557615419154 28.041577065127584 28.02307811072644 21.876787208726824 68-69 21.59267997550651 26.94350578838315 29.516634867398604 21.947179368711737 70-71 21.258921632389953 28.80166056854611 28.925414067040755 21.014003732023177 72-73 22.34690052514959 29.492399729339436 27.843585004156367 20.317114741354608 74-75 23.35567443263033 28.581205866088 27.527821748581815 20.535297952699857 76-77 23.393437183735696 27.28873167628793 27.931328429335817 21.386502710640563 78-79 23.24023196411279 27.384044322911453 28.656856992852937 20.71886672012282 80-81 22.2582870723445 28.71853218168597 28.1657503148112 20.857430431158328 82-83 21.454858110588212 28.70215615419455 28.515683419644905 21.32730231557233 84-85 21.629515881182527 27.900578995625914 28.977594939335926 21.492310183855633 86-87 20.780776849166426 28.511162539983108 29.4700032100912 21.238057400759264 88-89 20.76107127717486 29.126280679062045 29.271327398351314 20.841320645411777 90-91 21.530903460165877 28.107790517002844 28.63433595606859 21.726970066762682 92-93 21.41950969082805 28.57113597128617 27.743955368724947 22.26539896916083 94-95 21.131135704701475 29.74597778880595 27.221830955631432 21.901055550861145 96-97 20.99537750740451 30.58208610983051 26.494688553919488 21.927847828845493 98-99 21.378065155176966 29.12536119147443 27.355561769983904 22.1410118833647 100-101 22.024514633269945 29.048267780001087 27.115289416774047 21.811928169954925 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1376.0 1 991.0 2 509.0 3 459.5 4 493.5 5 508.5 6 647.0 7 937.5 8 1298.5 9 1678.5 10 2005.5 11 2214.5 12 2297.5 13 2100.5 14 1722.5 15 1469.5 16 1247.0 17 1063.0 18 1020.5 19 1024.5 20 1102.5 21 1228.5 22 1437.0 23 1762.5 24 2098.5 25 2603.5 26 3360.5 27 4273.0 28 5375.5 29 6859.5 30 8811.0 31 11925.5 32 18187.5 33 29672.5 34 48793.5 35 81219.0 36 124365.0 37 186016.0 38 267250.5 39 321423.0 40 339467.0 41 330335.5 42 341323.5 43 383909.5 44 381597.0 45 364904.5 46 380903.5 47 366117.0 48 303623.0 49 246664.0 50 198463.0 51 164566.0 52 147900.0 53 135691.0 54 110447.0 55 70934.0 56 46495.5 57 37850.5 58 33021.0 59 27980.5 60 22257.0 61 17384.5 62 12688.0 63 9027.0 64 6773.0 65 5114.5 66 4126.5 67 3415.5 68 2727.0 69 2062.5 70 1513.0 71 1170.5 72 883.5 73 659.0 74 496.0 75 348.0 76 275.5 77 218.0 78 162.0 79 124.0 80 98.5 81 83.0 82 68.5 83 56.0 84 46.0 85 37.0 86 27.5 87 20.0 88 20.0 89 17.5 90 11.0 91 10.0 92 8.0 93 5.0 94 4.5 95 3.5 96 2.5 97 1.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23379327646320136 2 0.22728723247208987 3 0.2087945096060502 4 0.1977625219689481 5 0.2015459279855184 6 0.127840228531867 7 0.10402598598831493 8 0.09893429938657551 9 0.09267576793860416 10-11 0.07336094983999199 12-13 0.10175417443163606 14-15 0.10412322305883427 16-17 0.0942050418658627 18-19 0.08782275414632128 20-21 0.08264267020774611 22-23 0.16105994771474322 24-25 0.624270832467776 26-27 0.3596180457152131 28-29 0.1839194990204691 30-31 0.3133685590827468 32-33 0.3515562085957924 34-35 0.33215299316034447 36-37 0.34228332796172184 38-39 0.3500534538695846 40-41 0.35202471448102185 42-43 0.37179919873118 44-45 0.3486214170128453 46-47 0.37187875633433215 48-49 0.35973296225309964 50-51 0.36628320491262895 52-53 0.3600953913341262 54-55 0.3425131610374948 56-57 0.3511230616452972 58-59 0.34162918766913725 60-61 0.3201132758833148 62-63 0.30787024473156294 64-65 0.30775532819367646 66-67 0.3203961473611892 68-69 0.3190525078412858 70-71 0.3049089339475652 72-73 0.2960073221282048 74-75 0.3026813210593042 76-77 0.3041929155191956 78-79 0.30743709778106776 80-81 0.3178591437940031 82-83 0.34527999768045387 84-85 0.33163144887301355 86-87 0.34240708423329186 88-89 0.34429878724157703 90-91 0.3252845200882064 92-93 0.3038039672371182 94-95 0.303830486438169 96-97 0.3121221566333627 98-99 0.33344359427814646 100-101 0.3408336116376155 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5656279.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.16779306223309 #Duplication Level Percentage of deduplicated Percentage of total 1 83.33991432543576 26.808611178448526 2 7.172756575293739 4.614634983996415 3 2.717816120140193 2.622784396016129 4 1.3980082574419053 1.798833612987372 5 0.885082502821072 1.423557539687579 6 0.5807922885578933 1.1209683690282646 7 0.4563412264898683 1.0275643097644207 8 0.35070615023604157 0.9025154293156331 9 0.3337746710305003 0.9663115092411653 >10 2.1522262548821836 14.148578615690187 >50 0.2936206809163652 6.633178602265648 >100 0.26686392118865077 17.9260282674141 >500 0.033464363349466494 7.496726858986694 >1k 0.017962843234727372 10.129938254047392 >5k 3.90731938152743E-4 0.8576853993021559 >10k+ 2.790870434305037E-4 1.5220826738082551 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 26995 0.4772572215762341 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 22884 0.4045769312298775 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 13715 0.24247389494047233 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 10826 0.19139791371677387 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 10351 0.1830001667173773 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 8335 0.14735836050520137 No Hit TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 7239 0.12798166427080418 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 7170 0.1267617810224708 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 6978 0.12336732328797784 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 6666 0.11785132946942681 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 6200 0.10961269767633455 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 3.889482820773162E-4 0.0 0.0 2 4.0662774944446694E-4 0.0 0.0 3 4.5966615154591916E-4 0.0 0.0 4 4.773456189130699E-4 0.0 0.0 5 5.303840210145221E-4 0.0 0.0 6 6.541402925845772E-4 0.0 0.0 7 7.602170967874817E-4 0.0 0.0 8 0.001007729639927592 0.0 0.0 9 0.001131485911497647 0.0 0.0 10-11 0.001803305671449375 0.0 0.0 12-13 0.0026077214366547335 0.0 0.0 14-15 0.0035270537397465718 0.0 0.0 16-17 0.00465853965124422 0.0 0.0 18-19 0.006081736774299853 0.0 0.0 20-21 0.007655209369976268 0.0 0.0 22-23 0.009511553443527096 0.0 0.0 24-25 0.011588890859167308 0.0 0.0 26-27 0.01394909975268193 0.0 0.0 28-29 0.01664521852617242 0.0 0.0 30-31 0.027146822142259955 0.0 8.839733683575368E-6 32-33 0.031115862566185298 0.0 1.7679467367150736E-5 34-35 0.03410369255123377 0.0 3.535893473430147E-5 36-37 0.03785173963306973 0.0 3.535893473430147E-5 38-39 0.04243956141484534 0.0 3.535893473430147E-5 40-41 0.047884837363927776 0.0 3.535893473430147E-5 42-43 0.053975413871911196 0.0 3.535893473430147E-5 44-45 0.06072897040616278 0.0 5.303840210145221E-5 46-47 0.06885268566136854 0.0 5.303840210145221E-5 48-49 0.07793993188808401 0.0 5.303840210145221E-5 50-51 0.08818518322734788 0.0 5.303840210145221E-5 52-53 0.09970335621704657 0.0 5.303840210145221E-5 54-55 0.1126093673950666 0.0 5.303840210145221E-5 56-57 0.12723912664138384 0.0 5.303840210145221E-5 58-59 0.14394622330334128 0.0 5.303840210145221E-5 60-61 0.1621825938925573 0.0 5.303840210145221E-5 62-63 0.1826377376363507 0.0 5.303840210145221E-5 64-65 0.20646965964726988 0.0 5.303840210145221E-5 66-67 0.23307725803483173 0.0 7.071786946860294E-5 68-69 0.262195340788529 0.0 8.839733683575368E-5 70-71 0.2954857778408738 0.0 8.839733683575368E-5 72-73 0.3329662486592334 0.0 8.839733683575368E-5 74-75 0.37506990019410286 1.7679467367150736E-5 8.839733683575368E-5 76-77 0.42139010469603777 1.7679467367150736E-5 9.723707051932906E-5 78-79 0.4751091662911253 1.7679467367150736E-5 1.0607680420290442E-4 80-81 0.5353961500131093 1.7679467367150736E-5 1.2375627157005517E-4 82-83 0.6021803379925212 1.7679467367150736E-5 1.2375627157005517E-4 84-85 0.6779191761933949 1.7679467367150736E-5 1.2375627157005517E-4 86-87 0.7600049431790759 1.7679467367150736E-5 1.2375627157005517E-4 88-89 0.8500464704799746 1.7679467367150736E-5 1.2375627157005517E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 885 0.0 63.179573 9 CGGGAGA 1375 0.0 55.884148 4 AGAGCGG 1040 0.0 54.67463 8 TTGTGTT 70190 0.0 49.240128 1 TCGGGAG 2350 0.0 35.92794 3 CGGAAAG 560 0.0 35.572197 5 TCGGGAA 485 0.0 35.207966 3 TCGGGTA 465 0.0 35.154354 14-15 ACGTGGC 180 0.0 34.263813 1 TGTGTTT 104005 0.0 33.35375 2 GATCCGG 580 0.0 32.71876 1 TCGGAAA 440 0.0 31.262354 4 GCCGTAT 530 0.0 30.465939 58-59 CGAGTCC 145 2.4321707E-8 29.446886 1 CGACGCC 145 2.4321707E-8 29.446886 1 GATCTCG 1245 0.0 29.386555 46-47 CCGGATC 540 0.0 29.018427 60-61 GTGTTTG 147200 0.0 28.8553 1 GGCGCCG 1050 0.0 28.723688 56-57 ATCTCGG 1350 0.0 28.508774 46-47 >>END_MODULE