FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n02_34310-1.34200000035810.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n02_34310-1.34200000035810.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5656279
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA269950.4772572215762341No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC228840.4045769312298775No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT137150.24247389494047233No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC108260.19139791371677387No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT103510.1830001667173773No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC83350.14735836050520137No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT72390.12798166427080418No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC71700.1267617810224708No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG69780.12336732328797784No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT66660.11785132946942681No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA62000.10961269767633455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC8850.063.1795739
CGGGAGA13750.055.8841484
AGAGCGG10400.054.674638
TTGTGTT701900.049.2401281
TCGGGAG23500.035.927943
CGGAAAG5600.035.5721975
TCGGGAA4850.035.2079663
TCGGGTA4650.035.15435414-15
ACGTGGC1800.034.2638131
TGTGTTT1040050.033.353752
GATCCGG5800.032.718761
TCGGAAA4400.031.2623544
GCCGTAT5300.030.46593958-59
CGAGTCC1452.4321707E-829.4468861
CGACGCC1452.4321707E-829.4468861
GATCTCG12450.029.38655546-47
CCGGATC5400.029.01842760-61
GTGTTTG1472000.028.85531
GGCGCCG10500.028.72368856-57
ATCTCGG13500.028.50877446-47
Produced by FastQC (version 0.11.2)