FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n02_34302-1.342000000358e7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n02_34302-1.342000000358e7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8204716
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT224750.2739278239490556Illumina Single End PCR Primer 1 (96% over 32bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC165520.20173763479442797No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG157000.19135336311457948No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA127780.15573969897312717No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA126820.15456964019229916No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTCTTCGCCTGTGTAGATCT122700.149548137924579Illumina Single End PCR Primer 1 (96% over 32bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC113220.13799380746390247No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG105180.12819456517446795No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC100720.12275866708853786No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA93560.114031978681529No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAGA83600.084.702724
GAGCGGC60500.084.649879
AGAGCGG63950.083.41898
GAGAGCG75250.081.9730457
AAGCGGC27900.078.450159
TCGGGAG89600.076.5451053
CGGAAAG33450.076.280135
TCGGGAA31800.070.3998953
GAACCGG10000.069.738931
ATTCGGA9850.069.344592
GATCCGG16600.068.018461
AGAGCGT212700.066.777598
GAGCGTC214750.065.719369
GATCGGG133500.065.6006851
AAGCGTC12050.064.8713469
ACCGGAA12600.063.9934463
ATCGGGA133700.063.9662132
AACCGGA10550.063.8443072
ATCCGGA17350.063.700492
AAGAGCG214400.063.596417
Produced by FastQC (version 0.11.2)