##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n02_34302-1.342000000358e7.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8204716 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.116218160384832 33.0 33.0 33.0 27.0 33.0 2 30.79281586346194 33.0 33.0 33.0 27.0 33.0 3 30.61557755320233 33.0 33.0 33.0 27.0 33.0 4 34.06405169904723 37.0 37.0 37.0 27.0 37.0 5 34.149098762224064 37.0 37.0 37.0 27.0 37.0 6 34.542368681621646 37.0 37.0 37.0 27.0 37.0 7 33.98738725386717 37.0 37.0 37.0 27.0 37.0 8 34.34146581063866 37.0 37.0 37.0 27.0 37.0 9 33.85532015977152 37.0 37.0 37.0 27.0 37.0 10-11 33.8423431719026 37.0 37.0 37.0 24.5 37.0 12-13 33.81812179726879 37.0 37.0 37.0 24.5 37.0 14-15 35.29802670805425 40.0 37.0 40.0 22.0 40.0 16-17 35.19012114496102 40.0 37.0 40.0 24.5 40.0 18-19 35.22528939453846 40.0 37.0 40.0 22.0 40.0 20-21 35.280529149333134 40.0 37.0 40.0 24.5 40.0 22-23 35.817393070034356 40.0 37.0 40.0 27.0 40.0 24-25 35.14396037595939 37.0 37.0 40.0 22.0 40.0 26-27 35.06866038995134 37.0 37.0 40.0 21.0 40.0 28-29 34.86111956830681 37.0 37.0 40.0 18.5 40.0 30-31 34.82959385797144 37.0 37.0 40.0 18.5 40.0 32-33 34.63803628303527 37.0 37.0 40.0 15.0 40.0 34-35 34.71981486013654 37.0 37.0 40.0 18.5 40.0 36-37 34.58208139075137 37.0 37.0 40.0 18.5 40.0 38-39 34.670730284875184 37.0 37.0 40.0 18.5 40.0 40-41 34.29085296797598 37.0 35.0 40.0 15.0 40.0 42-43 34.19338652306795 37.0 33.0 40.0 15.0 40.0 44-45 34.08275953731976 37.0 33.0 40.0 15.0 40.0 46-47 34.017086331812095 37.0 33.0 40.0 15.0 40.0 48-49 33.89641725563688 37.0 33.0 40.0 15.0 40.0 50-51 32.90242581217924 37.0 33.0 38.5 15.0 38.5 52-53 33.39987136666278 37.0 33.0 38.5 18.5 40.0 54-55 34.05528411952345 37.0 33.0 40.0 18.5 40.0 56-57 34.27447537489415 37.0 33.0 40.0 18.5 40.0 58-59 33.825172742115626 37.0 33.0 40.0 15.0 40.0 60-61 33.64687028777108 37.0 33.0 40.0 15.0 40.0 62-63 33.27393739161721 37.0 33.0 40.0 15.0 40.0 64-65 33.0743629639344 37.0 33.0 38.5 15.0 40.0 66-67 32.936350813361486 37.0 33.0 37.0 15.0 40.0 68-69 32.99235439715403 37.0 33.0 37.0 18.5 40.0 70-71 32.56347581074104 37.0 33.0 37.0 15.0 40.0 72-73 32.698611749632775 37.0 33.0 37.0 22.0 37.0 74-75 32.668941435632874 37.0 33.0 37.0 22.0 37.0 76-77 32.31647512235646 37.0 33.0 37.0 22.0 37.0 78-79 31.858731978047746 37.0 33.0 37.0 22.0 37.0 80-81 31.46406450875326 33.0 33.0 37.0 15.0 37.0 82-83 31.13446376449837 33.0 33.0 37.0 15.0 37.0 84-85 30.936358126228868 33.0 33.0 37.0 15.0 37.0 86-87 30.779266704661076 33.0 33.0 37.0 15.0 37.0 88-89 30.639153445408713 33.0 27.0 37.0 15.0 37.0 90-91 30.47479114450762 33.0 27.0 37.0 15.0 37.0 92-93 30.30131323253602 33.0 27.0 37.0 15.0 37.0 94-95 30.16383583539028 33.0 27.0 37.0 15.0 37.0 96-97 29.969888963859322 33.0 27.0 37.0 15.0 37.0 98-99 29.85243407571938 33.0 27.0 37.0 15.0 37.0 100-101 28.646548826309164 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.03361127255137575 2306 2 0.001987430012025726 2306 3 -0.010019883409160713 2306 4 0.15471203713806858 2306 5 0.11030954815414873 2306 6 0.0020297936745379275 2306 7 0.4369923762965371 2306 8 -0.013079871131857601 2306 9 0.23624895147457892 2306 10-11 0.20050272686439286 2306 12-13 0.17056926267135708 2306 14-15 0.5377344178499328 2306 16-17 0.546914222220444 2306 18-19 0.55564001248473 2306 20-21 0.5442875928670858 2306 22-23 0.4053809309674108 2306 24-25 0.5789314749082877 2306 26-27 0.4067141478848484 2306 28-29 0.4791015471276836 2306 30-31 0.4835535785539591 2306 32-33 0.5812479808076674 2306 34-35 0.46918185041094773 2306 36-37 0.5070893305910928 2306 38-39 0.4412646043309749 2306 40-41 0.6477450756124838 2306 42-43 0.52240710129022 2306 44-45 0.6385926389704508 2306 46-47 0.6173069345589468 2306 48-49 0.5589340740175004 2306 50-51 0.3737009618906697 2306 52-53 0.5131085352606917 2306 54-55 0.599928940889427 2306 56-57 0.6051353688751817 2306 58-59 0.767041460449498 2306 60-61 0.5329228419682153 2306 62-63 0.6216111464411611 2306 64-65 0.6561098973775259 2306 66-67 0.6169578733605832 2306 68-69 0.4608327016797773 2306 70-71 0.5698782265570657 2306 72-73 0.3324079765279748 2306 74-75 0.42081820194857755 2306 76-77 0.5879404068423568 2306 78-79 0.6368994219708561 2306 80-81 0.6253707051517843 2306 82-83 0.6654721675965476 2306 84-85 0.7011490293304945 2306 86-87 0.7248303177881148 2306 88-89 0.6868767264082436 2306 90-91 0.6791188137242052 2306 92-93 0.5820132267321441 2306 94-95 0.6000292523729662 2306 96-97 0.6946561571725383 2306 98-99 0.756067949299414 2306 100-101 0.5805597556907038 2307 1 0.08779779407478472 2307 2 0.05143510433210707 2307 3 0.09474839149537573 2307 4 0.17381301718219788 2307 5 0.09870822586747607 2307 6 0.10137158279329839 2307 7 0.5241952165633563 2307 8 0.06859654410553162 2307 9 0.23537799761771083 2307 10-11 0.29019090375125955 2307 12-13 0.2520575145052746 2307 14-15 0.5462138544884922 2307 16-17 0.5392169700486207 2307 18-19 0.560075313760592 2307 20-21 0.47772033494971566 2307 22-23 0.42643597226270913 2307 24-25 0.5991768197097329 2307 26-27 0.4050139429224586 2307 28-29 0.5249695633720108 2307 30-31 0.5383890715151196 2307 32-33 0.5382265531088137 2307 34-35 0.40332532231678186 2307 36-37 0.3844187571281523 2307 38-39 0.28032961980446913 2307 40-41 0.5113099122651619 2307 42-43 0.4245191761818816 2307 44-45 0.4942508255373568 2307 46-47 0.359543967397876 2307 48-49 0.4840080816651664 2307 50-51 0.4267054977648357 2307 52-53 0.32018805621839164 2307 54-55 0.3721756272521759 2307 56-57 0.4435509266244253 2307 58-59 0.5071035253428136 2307 60-61 0.3761182865541741 2307 62-63 0.6171733989968544 2307 64-65 0.5742013171764313 2307 66-67 0.4879027707637107 2307 68-69 0.3331959936507616 2307 70-71 0.33916689108612985 2307 72-73 0.22264128058096588 2307 74-75 0.3393562980013556 2307 76-77 0.4559077278001382 2307 78-79 0.536186982776929 2307 80-81 0.54009324996861 2307 82-83 0.5755273345895411 2307 84-85 0.5347203041217128 2307 86-87 0.4680388773736084 2307 88-89 0.516855346011809 2307 90-91 0.5807758276038513 2307 92-93 0.5826778330176481 2307 94-95 0.5819660371910409 2307 96-97 0.5981363917630382 2307 98-99 0.5896084996254274 2307 100-101 0.5092981236807894 2201 1 0.0888174566386013 2201 2 0.010044802518901719 2201 3 0.0790030981440033 2201 4 0.16047027133729586 2201 5 0.18850342910072726 2201 6 0.2141777054827969 2201 7 0.5158715864452077 2201 8 0.11753288315708232 2201 9 0.23704341504453197 2201 10-11 0.3113639478266421 2201 12-13 0.3412798352000763 2201 14-15 0.6047815970728081 2201 16-17 0.5710930140281576 2201 18-19 0.6279677675662114 2201 20-21 0.5272784654583589 2201 22-23 0.4545754534274735 2201 24-25 0.5865141631815476 2201 26-27 0.4140926560325582 2201 28-29 0.596185270166778 2201 30-31 0.6593703297746387 2201 32-33 0.6830457433185444 2201 34-35 0.5446057067369594 2201 36-37 0.5450205607476519 2201 38-39 0.5439349810076308 2201 40-41 0.7164858900899773 2201 42-43 0.6537448674672106 2201 44-45 0.6352553557996075 2201 46-47 0.6733862913296988 2201 48-49 0.5941737512970136 2201 50-51 0.583816343838059 2201 52-53 0.5352435570378589 2201 54-55 0.8034307047443292 2201 56-57 0.8410700069953378 2201 58-59 0.8778151756436543 2201 60-61 0.7051515634752903 2201 62-63 0.8348167512337739 2201 64-65 0.7649532495697713 2201 66-67 0.7148785732235368 2201 68-69 0.7668348014830997 2201 70-71 0.7291520730587351 2201 72-73 0.6153393558087714 2201 74-75 0.6537414520035583 2201 76-77 0.7207049345312626 2201 78-79 0.7725115384325285 2201 80-81 0.7971061996360298 2201 82-83 0.8757171954760778 2201 84-85 0.8652288233089358 2201 86-87 0.7187270592503587 2201 88-89 0.6954777880164364 2201 90-91 0.7351771981535329 2201 92-93 0.6346479392580342 2201 94-95 0.7065057040918425 2201 96-97 0.7430892663503599 2201 98-99 0.7660219084474136 2201 100-101 0.7664734745286239 1101 1 0.1120470698449445 1101 2 -0.010128673298645197 1101 3 0.0830652144839199 1101 4 0.15460166680664145 1101 5 0.09996546192898137 1101 6 0.14822930343766672 1101 7 0.3243098010505179 1101 8 0.09565352649948267 1101 9 0.2523353541264015 1101 10-11 0.26625601807629096 1101 12-13 0.27326160711488967 1101 14-15 0.454843266352988 1101 16-17 0.4168108380197495 1101 18-19 0.44050242225984704 1101 20-21 0.40255858695875446 1101 22-23 0.3791494890908851 1101 24-25 0.44530366196783433 1101 26-27 0.4079495560704558 1101 28-29 0.4093869213376351 1101 30-31 0.5323879678317454 1101 32-33 0.49212348594678446 1101 34-35 0.38010219266591605 1101 36-37 0.4442040053920451 1101 38-39 0.41400564632480297 1101 40-41 0.5616055542127114 1101 42-43 0.4668031720744992 1101 44-45 0.5307630863020165 1101 46-47 0.44937513677336227 1101 48-49 0.43296210217611275 1101 50-51 0.39943172042347896 1101 52-53 0.38829720760961806 1101 54-55 0.5364400237838538 1101 56-57 0.5970408263249567 1101 58-59 0.6316432664262734 1101 60-61 0.5314677704874029 1101 62-63 0.6117162161237033 1101 64-65 0.6152417108900252 1101 66-67 0.4604285890710855 1101 68-69 0.48285031922138444 1101 70-71 0.5329181447047873 1101 72-73 0.5790473342742644 1101 74-75 0.5672331719429522 1101 76-77 0.467126991066543 1101 78-79 0.6398607465467769 1101 80-81 0.6408609510640169 1101 82-83 0.631707824241758 1101 84-85 0.6805978820855643 1101 86-87 0.5728577001645689 1101 88-89 0.5774692424431969 1101 90-91 0.6590656098509164 1101 92-93 0.6277342096830267 1101 94-95 0.6835929473067104 1101 96-97 0.6593714219347113 1101 98-99 0.5938571067989677 1101 100-101 0.5679043966139048 2304 1 0.07577883575521938 2304 2 -0.06876201752481492 2304 3 -0.06853934118275617 2304 4 0.001761779257456908 2304 5 -0.05502757538797454 2304 6 0.09290776289003588 2304 7 0.2512889177645121 2304 8 0.11935571674217016 2304 9 0.07511333877923931 2304 10-11 0.154003869094268 2304 12-13 0.13433906913271443 2304 14-15 0.14846430978240477 2304 16-17 0.2576071572559684 2304 18-19 0.27259512188751955 2304 20-21 0.2047353631531479 2304 22-23 0.16335357387060867 2304 24-25 0.2485758529470985 2304 26-27 0.283310278508786 2304 28-29 0.3469904876330858 2304 30-31 0.33560022623660046 2304 32-33 0.33749719941119594 2304 34-35 0.314809071024456 2304 36-37 0.25036289022981606 2304 38-39 0.2958304855136973 2304 40-41 0.42955384944408337 2304 42-43 0.3957118901600083 2304 44-45 0.41293477317146454 2304 46-47 0.3559458350540936 2304 48-49 0.34331145518074635 2304 50-51 0.24828763660318742 2304 52-53 0.24098254630511207 2304 54-55 0.4924058485436831 2304 56-57 0.46478112777357694 2304 58-59 0.39167715103014444 2304 60-61 0.3739522171497285 2304 62-63 0.43837693213180273 2304 64-65 0.4476325423820384 2304 66-67 0.3672023372553568 2304 68-69 0.32749049922706774 2304 70-71 0.3329856600203911 2304 72-73 0.3664094521411059 2304 74-75 0.49959060194910876 2304 76-77 0.5749207497773412 2304 78-79 0.5616674811436972 2304 80-81 0.4951467031996408 2304 82-83 0.5411958471680904 2304 84-85 0.4783500352353336 2304 86-87 0.5604998627402402 2304 88-89 0.5183025333903011 2304 90-91 0.5599085423193557 2304 92-93 0.5659039936799459 2304 94-95 0.45210969933839706 2304 96-97 0.6018249762627725 2304 98-99 0.5532871236689729 2304 100-101 0.39907369655720615 2202 1 0.0966405575896907 2202 2 -0.045339960610775165 2202 3 0.02830904628237363 2202 4 0.011890386490314597 2202 5 0.029718831808402513 2202 6 0.1902989284904848 2202 7 0.09080276187120973 2202 8 0.14508973454913843 2202 9 0.09252807457122003 2202 10-11 0.21575714719926964 2202 12-13 0.15503529561126328 2202 14-15 0.2052682615258874 2202 16-17 0.3050274883162345 2202 18-19 0.31238998847514665 2202 20-21 0.3042848368992921 2202 22-23 0.3378296394727158 2202 24-25 0.3402953999217502 2202 26-27 0.36387563279485136 2202 28-29 0.3428715039818897 2202 30-31 0.3590906768873978 2202 32-33 0.29256831422774354 2202 34-35 0.3538346926589213 2202 36-37 0.28168987386531086 2202 38-39 0.3497430531756507 2202 40-41 0.462043526604468 2202 42-43 0.3838811920936962 2202 44-45 0.4013840619650111 2202 46-47 0.3101407716986557 2202 48-49 0.3670710604447365 2202 50-51 0.36839238161103083 2202 52-53 0.3505725023428994 2202 54-55 0.3816709483214993 2202 56-57 0.4200410054175734 2202 58-59 0.3754645711719533 2202 60-61 0.37592321000585827 2202 62-63 0.41027637445277065 2202 64-65 0.4500202027063338 2202 66-67 0.4480704989818989 2202 68-69 0.3796139197664985 2202 70-71 0.4135695311334828 2202 72-73 0.4781559287610051 2202 74-75 0.5213090330566317 2202 76-77 0.4946395695245016 2202 78-79 0.5675433580529123 2202 80-81 0.5683083286164283 2202 82-83 0.5105147665991225 2202 84-85 0.48575875762459475 2202 86-87 0.47816090536273137 2202 88-89 0.3565966276630199 2202 90-91 0.3479252446139114 2202 92-93 0.4837276560126078 2202 94-95 0.4739248143192505 2202 96-97 0.5283261716667731 2202 98-99 0.4976427469291451 2202 100-101 0.5203712225051014 1102 1 -0.07891789698707896 1102 2 -0.007559518455632741 1102 3 -0.006730688367330373 1102 4 -0.06338073747729567 1102 5 -0.05502660469215925 1102 6 0.03782474999162844 1102 7 0.04345129634580758 1102 8 0.13163501874741002 1102 9 0.060714037123446474 1102 10-11 0.11040932499813039 1102 12-13 0.0485886437069567 1102 14-15 0.09072307387895506 1102 16-17 0.1324945038862566 1102 18-19 0.22526931467016453 1102 20-21 0.12994491760579052 1102 22-23 0.1597908363626388 1102 24-25 0.11360546310704933 1102 26-27 0.2612442473475127 1102 28-29 0.17268770500900388 1102 30-31 0.167146407849863 1102 32-33 0.21085988512064802 1102 34-35 0.233237262126039 1102 36-37 0.13640685590022628 1102 38-39 0.1726722091112194 1102 40-41 0.20431879499565042 1102 42-43 0.2893194992723025 1102 44-45 0.2581367034495372 1102 46-47 0.18956817017128458 1102 48-49 0.17626009993279723 1102 50-51 0.13016618171477745 1102 52-53 0.24227114499380065 1102 54-55 0.3248350153593904 1102 56-57 0.34999317050419165 1102 58-59 0.34984970371231583 1102 60-61 0.32907356154364464 1102 62-63 0.4594766172985203 1102 64-65 0.36805399322832244 1102 66-67 0.40099911739874017 1102 68-69 0.28453520410981525 1102 70-71 0.36819877692666125 1102 72-73 0.4465995121830417 1102 74-75 0.2747667379090828 1102 76-77 0.29478346078897744 1102 78-79 0.22238315062279312 1102 80-81 0.3052645275391903 1102 82-83 0.3370202031677394 1102 84-85 0.45258436182674444 1102 86-87 0.37562296098002435 1102 88-89 0.33010253693975145 1102 90-91 0.3481930735545049 1102 92-93 0.3707608115606895 1102 94-95 0.36271556572204844 1102 96-97 0.5191120320213223 1102 98-99 0.5386787927436032 1102 100-101 0.4867426473077465 2305 1 0.09063979151859414 2305 2 0.06762199218493947 2305 3 0.037001680374626034 2305 4 -5.396387274316794E-4 2305 5 0.02286070698352205 2305 6 0.2928853872150512 2305 7 -0.004226010507302647 2305 8 0.22764451459432422 2305 9 0.09374667161782213 2305 10-11 0.13811951149737212 2305 12-13 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12 81506.0 13 56911.0 14 41901.0 15 34129.0 16 30217.0 17 29432.0 18 30179.0 19 31519.0 20 33106.0 21 36003.0 22 39220.0 23 42125.0 24 46392.0 25 53155.0 26 62645.0 27 75795.0 28 91691.0 29 111703.0 30 135719.0 31 176134.0 32 240349.0 33 342457.0 34 518345.0 35 871658.0 36 1633877.0 37 2349543.0 38 546426.0 39 225.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.91974233045341 23.653552741834577 25.57449291877868 13.852212008933332 2 29.069392016205114 25.59411692344678 33.47225654548948 11.864234514858618 3 36.49800081074126 19.863014284215495 28.73274106147991 14.906243843563333 4 26.61752764772023 18.06331562102757 36.59179756313421 18.727359168117996 5 34.33479686518975 16.95266214738972 32.90616489641231 15.806376091008225 6 27.594326095037445 17.62020510389801 40.02773395998138 14.757734841083165 7 33.462016959055696 19.662961308654157 31.018069186827418 15.856952545462729 8 26.497630860232345 30.599040143980844 27.377405343600298 15.525923652186515 9 33.2473855167031 26.21430772522691 23.844236498657718 16.694070259412275 10-11 26.586251475890172 29.343364639900482 27.288964552473754 16.78141933173559 12-13 28.3196327600557 24.04361547754442 27.06183887891579 20.574912883484085 14-15 25.1955414648805 24.705520352252506 28.881842060168882 21.21709612269811 16-17 25.903191044149583 26.198470699156275 29.949673328315736 17.948664928378413 18-19 25.31312722348218 26.6187066156606 30.09780988587312 17.970356274984105 20-21 24.442300450946117 28.7309603679976 28.7661026699245 18.060636511131793 22-23 27.213723914813453 26.62341177976435 28.13024832699015 18.032615978432048 24-25 23.720998232239328 29.75562024139322 28.32582896667134 18.197552559696113 26-27 22.562476812360575 30.70742529222389 28.33487642371953 18.395221471696004 28-29 23.18719438470084 29.601492180473198 28.074590952016614 19.136722482809347 30-31 25.09737260254812 27.437280012989536 28.377920872411565 19.087426512050776 32-33 22.37053753968884 26.955019743053484 29.986550530242535 20.687892187015148 34-35 20.16477620730523 27.156523578254454 33.63086512247544 19.04783509196488 36-37 21.530812806770218 27.845755632684277 29.748105520209762 20.875326040335747 38-39 20.489799437921036 27.45124923594015 27.72232650910802 24.336624817030792 40-41 22.32273907712721 26.965449268612012 28.882242081644993 21.829569572615785 42-43 23.821463822590566 27.116883835030016 30.416897683825635 18.644754658553783 44-45 21.534816313705498 29.356092272395422 30.6282116404472 18.48087977345188 46-47 23.160694772518962 29.73461687339643 28.678622688940337 18.42606566514427 48-49 20.946299983577976 28.710626517011168 30.263446563745127 20.07962693566573 50-51 20.143705970381685 27.848255342334777 30.491576884076505 21.516461803207036 52-53 24.508102892458968 27.445803550393137 27.97025118832066 20.075842368827235 54-55 25.406630841863308 27.38895619594695 27.991423670249393 19.21298929194035 56-57 25.609872743322544 27.038512143514403 27.45921715879081 19.89239795437224 58-59 24.05225472804023 26.732569068493365 27.892364506264002 21.322811697202404 60-61 20.85022156248013 27.020271988731125 27.82292242378593 24.306584025002813 62-63 23.380393411100076 27.206714435848795 28.32993574536405 21.08295640768708 64-65 21.096443423717396 28.63967880107906 30.16385155064183 20.100026224561717 66-67 20.415256011323716 28.5865025594774 28.489702908892777 22.5085385203061 68-69 20.158776865217636 27.131470959065258 32.673242569401296 20.03650960631581 70-71 19.778662711807836 31.50702298376592 29.619593422353518 19.09472088207273 72-73 19.75413530443002 34.34357294850234 27.0105214285985 18.891770318469142 74-75 20.545806710078075 33.73609172986554 26.674862058066083 19.0432395019903 76-77 20.74594201927605 32.778099985280605 27.239371349797864 19.236586645645477 78-79 20.70891529602272 32.49319402804424 27.737030753182 19.060859922751032 80-81 19.94147712575815 33.01251089516717 27.4955534390335 19.55045854004118 82-83 19.260299040278134 32.42576064767314 27.98623520909349 20.32770510295524 84-85 19.405675966821203 31.645466139505864 28.31206799332452 20.63678990034841 86-87 19.335923060341862 31.462119509633506 28.988364573635234 20.213592856389397 88-89 19.598139786999752 31.707429532804603 28.602141621308856 20.092289058886788 90-91 20.181736565314278 30.874318409926417 28.188928110956113 20.75501691380319 92-93 19.762864091245426 31.690200034903494 27.844513809954464 20.702422063896616 94-95 19.49030796224411 32.23196096093453 27.742189692927766 20.535541383893598 96-97 20.046640081970736 32.450313555425645 27.209316670286704 20.29372969231692 98-99 20.447314878649422 31.34522508365447 27.11158260965271 21.095877428043394 100-101 20.494030177212196 31.40872261330458 26.742823618891638 21.354423590591594 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1858.0 1 1347.5 2 724.0 3 716.0 4 827.0 5 921.5 6 1242.0 7 1742.0 8 2298.5 9 2759.5 10 3156.5 11 3413.5 12 3334.5 13 3109.5 14 2820.5 15 2608.0 16 2411.5 17 2265.0 18 2381.5 19 2634.0 20 3121.0 21 3860.0 22 4906.0 23 6530.5 24 8626.0 25 11190.0 26 14909.5 27 20001.0 28 25524.0 29 31595.0 30 38720.0 31 46793.0 32 58615.0 33 82820.5 34 122883.0 35 198088.0 36 298825.5 37 401067.0 38 518202.5 39 581491.5 40 577519.0 41 527874.0 42 473866.5 43 478081.0 44 470325.0 45 445425.5 46 448810.0 47 408719.5 48 324452.5 49 273906.0 50 234713.0 51 200185.5 52 178578.5 53 153484.5 54 120479.0 55 87768.5 56 65943.0 57 51435.0 58 40447.0 59 31235.0 60 23520.0 61 17721.5 62 13107.0 63 9595.5 64 7292.0 65 5464.5 66 3974.5 67 2853.5 68 2138.5 69 1510.0 70 1051.5 71 832.5 72 605.0 73 438.0 74 355.0 75 289.5 76 237.0 77 185.5 78 156.0 79 131.0 80 108.0 81 77.0 82 64.0 83 64.0 84 54.0 85 47.5 86 40.5 87 33.0 88 25.5 89 19.0 90 16.0 91 11.5 92 9.5 93 7.5 94 7.0 95 6.0 96 4.5 97 2.0 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23392643937949834 2 0.22741798741114255 3 0.2089895616131015 4 0.198020260542839 5 0.20150606066072244 6 0.1271220112920423 7 0.10222169786254637 8 0.09835806626335392 9 0.0913377135783859 10-11 0.0713065510128565 12-13 0.10021065933299823 14-15 0.10282500942141082 16-17 0.0922822922816585 18-19 0.08689514664492957 20-21 0.08067311531563068 22-23 0.1598775630990762 24-25 0.627236823309911 26-27 0.3603171639335231 28-29 0.18391861461140155 30-31 0.3147458120427325 32-33 0.3525899007351382 34-35 0.3337409850627371 36-37 0.3436682025313247 38-39 0.35124311432595595 40-41 0.35379652385286703 42-43 0.37441880986496057 44-45 0.3509323174622985 46-47 0.37490633435697224 48-49 0.36221241539621846 50-51 0.36865383274692265 52-53 0.362401331136873 54-55 0.34441167738164247 56-57 0.3538818406389691 58-59 0.3440825983495346 60-61 0.322204936770511 62-63 0.3093830426306041 64-65 0.3091027160476974 66-67 0.32278996616092503 68-69 0.3207972097998273 70-71 0.30559253970521344 72-73 0.29693897997200636 74-75 0.3036729120179175 76-77 0.30509282710090146 78-79 0.308584721274935 80-81 0.3193833887729935 82-83 0.347275883772211 84-85 0.3330401686054703 86-87 0.3434427224537693 88-89 0.34541719664641657 90-91 0.3263976474018113 92-93 0.3049282875848475 94-95 0.30484906485489566 96-97 0.3132283920613462 98-99 0.33491104384356507 100-101 0.34187045596703164 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8204716.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.84329737323095 #Duplication Level Percentage of deduplicated Percentage of total 1 91.58024010594991 39.23599460371104 2 3.668809849388589 3.1436782276638793 3 1.4090531041424068 1.8110544346634192 4 0.6431015548375851 1.102103646203754 5 0.420735143664622 0.9012840437696225 6 0.33018110177190313 0.8487628278140807 7 0.23716128585604435 0.7112540046743837 8 0.20143916464783024 0.6904254426897777 9 0.14332925230723942 0.5526628000993716 >10 1.0355121112643446 9.20960756514502 >50 0.1420492100417984 4.222643729040774 >100 0.14464919628698986 13.565485398265201 >500 0.024625282615408 7.262666502528885 >1k 0.018507287200084085 14.288028978512028 >5k 3.466615602898138E-4 0.9168836108076802 >10k+ 2.596884649664348E-4 1.5374641844112371 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT 22475 0.2739278239490556 Illumina Single End PCR Primer 1 (96% over 32bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 16552 0.20173763479442797 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 15700 0.19135336311457948 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 12778 0.15573969897312717 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 12682 0.15456964019229916 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTCTTCGCCTGTGTAGATCT 12270 0.149548137924579 Illumina Single End PCR Primer 1 (96% over 32bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 11322 0.13799380746390247 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 10518 0.12819456517446795 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 10072 0.12275866708853786 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 9356 0.114031978681529 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.002413246235457754 0.0 0.0 2 0.0026570084814635875 0.0 0.0 3 0.0028763945028688377 0.0 0.0 4 0.0030957805242740884 0.0 0.0 5 0.0033639189948805053 0.0 0.0 6 0.0037905029253907144 0.0 0.0 7 0.004217086855900923 0.0 0.0 8 0.004850868695516091 0.0 0.0 9 0.005496838647431551 0.0 0.0 10-11 0.00878153491236016 0.0 0.0 12-13 0.01156651857297681 0.0 0.0 14-15 0.014771992107953524 0.0 0.0 16-17 0.01726446107336317 0.0 1.828216845043753E-5 18-19 0.020445558383739303 0.0 2.4376224600583373E-5 20-21 0.02412636829842739 0.0 2.4376224600583373E-5 22-23 0.028709098523337064 0.0 2.4376224600583373E-5 24-25 0.03401092737396395 0.0 2.4376224600583373E-5 26-27 0.03936760272994215 0.0 2.4376224600583373E-5 28-29 0.0451447679602804 0.0 2.4376224600583373E-5 30-31 0.0701364922320285 0.0 3.0470280750729215E-5 32-33 0.07698621134479244 0.0 4.26583930510209E-5 34-35 0.08331184162864383 0.0 4.8752449201166745E-5 36-37 0.08946074428414097 0.0 4.8752449201166745E-5 38-39 0.09681627005736701 0.0 4.8752449201166745E-5 40-41 0.1044582164696499 0.0 4.8752449201166745E-5 42-43 0.1120757866573322 0.0 5.484650535131259E-5 44-45 0.12096092052424484 0.0 7.312867380175012E-5 46-47 0.13025435615321726 1.2188112300291686E-5 7.312867380175012E-5 48-49 0.14062034566461534 2.4376224600583373E-5 8.53167861020418E-5 50-51 0.1517541862509318 2.4376224600583373E-5 8.53167861020418E-5 52-53 0.16299771984795086 2.4376224600583373E-5 8.53167861020418E-5 54-55 0.1751005153621405 2.4376224600583373E-5 8.53167861020418E-5 56-57 0.18799553817584913 2.4376224600583373E-5 8.53167861020418E-5 58-59 0.20134152114466852 3.0470280750729215E-5 8.53167861020418E-5 60-61 0.21672901292378677 3.656433690087506E-5 9.141084225218764E-5 62-63 0.23374361769499397 3.656433690087506E-5 1.1578706685277102E-4 64-65 0.2514651329796181 3.656433690087506E-5 1.2188112300291686E-4 66-67 0.271142840288439 3.656433690087506E-5 1.279751791530627E-4 68-69 0.29314238299046547 3.656433690087506E-5 1.3406923530320854E-4 70-71 0.3176161124894512 3.656433690087506E-5 1.401632914533544E-4 72-73 0.34415572702333636 3.656433690087506E-5 1.5844545990379192E-4 74-75 0.37273685036752036 3.656433690087506E-5 1.706335722040836E-4 76-77 0.4048708084472394 3.656433690087506E-5 1.706335722040836E-4 78-79 0.4415509324149672 3.656433690087506E-5 1.706335722040836E-4 80-81 0.48296613801135835 3.656433690087506E-5 1.8282168450437528E-4 82-83 0.5286167126321009 3.656433690087506E-5 1.8282168450437528E-4 84-85 0.5792583192398129 3.656433690087506E-5 1.8891574065452112E-4 86-87 0.6352626952596532 3.656433690087506E-5 2.0719790910495866E-4 88-89 0.7006214474699672 3.656433690087506E-5 2.0719790910495866E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGAGA 8360 0.0 84.70272 4 GAGCGGC 6050 0.0 84.64987 9 AGAGCGG 6395 0.0 83.4189 8 GAGAGCG 7525 0.0 81.973045 7 AAGCGGC 2790 0.0 78.45015 9 TCGGGAG 8960 0.0 76.545105 3 CGGAAAG 3345 0.0 76.28013 5 TCGGGAA 3180 0.0 70.399895 3 GAACCGG 1000 0.0 69.73893 1 ATTCGGA 985 0.0 69.34459 2 GATCCGG 1660 0.0 68.01846 1 AGAGCGT 21270 0.0 66.77759 8 GAGCGTC 21475 0.0 65.71936 9 GATCGGG 13350 0.0 65.600685 1 AAGCGTC 1205 0.0 64.871346 9 ACCGGAA 1260 0.0 63.993446 3 ATCGGGA 13370 0.0 63.966213 2 AACCGGA 1055 0.0 63.844307 2 ATCCGGA 1735 0.0 63.70049 2 AAGAGCG 21440 0.0 63.59641 7 >>END_MODULE