##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n02_34298-1.342000000357ce.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5257116 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.690847034762026 33.0 33.0 33.0 27.0 33.0 2 31.57801235506312 33.0 33.0 33.0 27.0 33.0 3 31.516061467922718 33.0 33.0 33.0 27.0 33.0 4 35.161411123513346 37.0 37.0 37.0 33.0 37.0 5 35.196121789970015 37.0 37.0 37.0 33.0 37.0 6 35.30367848074876 37.0 37.0 37.0 33.0 37.0 7 35.086982482410505 37.0 37.0 37.0 33.0 37.0 8 35.159875871104994 37.0 37.0 37.0 33.0 37.0 9 34.99604669176027 37.0 37.0 37.0 33.0 37.0 10-11 34.9075563483857 37.0 37.0 37.0 33.0 37.0 12-13 34.88393274943905 37.0 37.0 37.0 33.0 37.0 14-15 36.434970238434914 40.0 37.0 40.0 33.0 40.0 16-17 36.34499153147848 40.0 37.0 40.0 33.0 40.0 18-19 36.342100022141416 40.0 37.0 40.0 33.0 40.0 20-21 36.355507468353366 40.0 37.0 40.0 33.0 40.0 22-23 36.48114802869101 40.0 37.0 40.0 33.0 40.0 24-25 36.04574713588211 38.5 37.0 40.0 27.0 40.0 26-27 35.96110443444657 37.0 37.0 40.0 27.0 40.0 28-29 35.90821650121474 37.0 37.0 40.0 27.0 40.0 30-31 35.87833585943319 37.0 37.0 40.0 27.0 40.0 32-33 35.73796678635206 37.0 37.0 40.0 27.0 40.0 34-35 35.71072456837551 37.0 37.0 40.0 27.0 40.0 36-37 35.60632550242376 37.0 37.0 40.0 27.0 40.0 38-39 35.564576090769165 37.0 37.0 40.0 27.0 40.0 40-41 35.326780215616324 37.0 37.0 40.0 27.0 40.0 42-43 35.238714724955656 37.0 37.0 40.0 27.0 40.0 44-45 35.13599966217219 37.0 37.0 40.0 27.0 40.0 46-47 35.03208955252271 37.0 37.0 40.0 27.0 40.0 48-49 34.88027323346108 37.0 35.0 40.0 24.5 40.0 50-51 33.769538088944586 37.0 33.0 38.5 22.0 38.5 52-53 34.29073744996306 37.0 33.0 38.5 24.5 40.0 54-55 35.03029360584777 37.0 37.0 40.0 27.0 40.0 56-57 35.13230257806752 37.0 37.0 40.0 27.0 40.0 58-59 34.810266408426216 37.0 33.0 40.0 27.0 40.0 60-61 34.56618495768402 37.0 33.0 40.0 27.0 40.0 62-63 34.25518211886517 37.0 33.0 40.0 27.0 40.0 64-65 34.012747864038005 37.0 33.0 37.0 24.5 40.0 66-67 33.7975721859666 37.0 33.0 37.0 22.0 40.0 68-69 33.659572663034254 37.0 33.0 37.0 22.0 40.0 70-71 33.26816737542029 37.0 33.0 37.0 22.0 40.0 72-73 33.105780812141106 37.0 33.0 37.0 22.0 37.0 74-75 32.93193606532555 37.0 33.0 37.0 22.0 37.0 76-77 32.62966871950324 37.0 33.0 37.0 22.0 37.0 78-79 32.30491100063229 37.0 33.0 37.0 22.0 37.0 80-81 32.05463984435573 35.0 33.0 37.0 22.0 37.0 82-83 31.81967574997394 33.0 33.0 37.0 22.0 37.0 84-85 31.645637836410685 33.0 33.0 37.0 18.5 37.0 86-87 31.49743490537397 33.0 33.0 37.0 15.0 37.0 88-89 31.359671823866925 33.0 33.0 37.0 15.0 37.0 90-91 31.188370962329916 33.0 33.0 37.0 15.0 37.0 92-93 30.995657409880245 33.0 33.0 37.0 15.0 37.0 94-95 30.85955740752154 33.0 30.0 37.0 15.0 37.0 96-97 30.651582445584232 33.0 27.0 37.0 15.0 37.0 98-99 30.527730413405372 33.0 27.0 37.0 15.0 37.0 100-101 29.25530262219818 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.2472197599523014 2306 2 0.24689464351778767 2306 3 0.1793383253072811 2306 4 0.34202888811521603 2306 5 0.33096235441637845 2306 6 0.3083423161047065 2306 7 0.47236468006120447 2306 8 0.36323377911843835 2306 9 0.4174417069287557 2306 10-11 0.4698616327769969 2306 12-13 0.46974541283502447 2306 14-15 0.7569942815235322 2306 16-17 0.7527465146806875 2306 18-19 0.7673157118235707 2306 20-21 0.7846821333864682 2306 22-23 0.7281395159105841 2306 24-25 0.7846543397785055 2306 26-27 0.747482266149909 2306 28-29 0.7306892887636423 2306 30-31 0.8444332930124219 2306 32-33 0.7953773346867976 2306 34-35 0.7329797061531451 2306 36-37 0.769035543274363 2306 38-39 0.7799321630093061 2306 40-41 0.9399251830954825 2306 42-43 0.7970091824013608 2306 44-45 0.8777545466904968 2306 46-47 0.8460513641506964 2306 48-49 0.8876055970175258 2306 50-51 0.8632593375108968 2306 52-53 0.7903006164759674 2306 54-55 0.9121956557888424 2306 56-57 0.8755636111314047 2306 58-59 0.9858303303177607 2306 60-61 0.8620396379601587 2306 62-63 0.8693143523663949 2306 64-65 1.0265097506005105 2306 66-67 0.9454093575411093 2306 68-69 0.908129500650432 2306 70-71 0.900740074308942 2306 72-73 0.861064498470256 2306 74-75 0.8129113856385075 2306 76-77 0.9765850942621555 2306 78-79 0.9459723367876194 2306 80-81 0.9151460866437446 2306 82-83 0.8748322455124615 2306 84-85 0.8454121604250879 2306 86-87 0.8412310326597812 2306 88-89 0.862720308229342 2306 90-91 0.9852892041636387 2306 92-93 0.8512192750100063 2306 94-95 0.8331170608268827 2306 96-97 0.9387926571473955 2306 98-99 0.9893640112776154 2306 100-101 0.7333787878637779 2307 1 0.0808981224978993 2307 2 0.12016843932507726 2307 3 0.09586665109261006 2307 4 0.18359981348638144 2307 5 0.0642063344325905 2307 6 0.21683762192035516 2307 7 0.3782226521798364 2307 8 0.20005173505941798 2307 9 0.1886818525903351 2307 10-11 0.2825773936048108 2307 12-13 0.2710421332547668 2307 14-15 0.5255267081558372 2307 16-17 0.4039183755967528 2307 18-19 0.4334439071844045 2307 20-21 0.3281681981880027 2307 22-23 0.3716285591560222 2307 24-25 0.4337342357524321 2307 26-27 0.47097497758415585 2307 28-29 0.4814836711049182 2307 30-31 0.4423055867434229 2307 32-33 0.40867483263716053 2307 34-35 0.33758767239326204 2307 36-37 0.2139250821955727 2307 38-39 0.22133833340451048 2307 40-41 0.3799198846005396 2307 42-43 0.3361863794348352 2307 44-45 0.3625690288253196 2307 46-47 0.2648152472263945 2307 48-49 0.3950559393034325 2307 50-51 0.28478439484594276 2307 52-53 0.2875881946405272 2307 54-55 0.32167808061840475 2307 56-57 0.39732114385117967 2307 58-59 0.3873943268878506 2307 60-61 0.3751214338677826 2307 62-63 0.38029775616345063 2307 64-65 0.43511682644266614 2307 66-67 0.38868865563161137 2307 68-69 0.25624359699838806 2307 70-71 0.29209161377253423 2307 72-73 0.27305367574567185 2307 74-75 0.25918496041391137 2307 76-77 0.3573846340141884 2307 78-79 0.46179947417382294 2307 80-81 0.456898383572657 2307 82-83 0.4637146549795226 2307 84-85 0.3903858795766375 2307 86-87 0.2609423744174073 2307 88-89 0.38581720014474286 2307 90-91 0.4523510633083241 2307 92-93 0.41650300314391586 2307 94-95 0.41928548085644834 2307 96-97 0.3844674079378585 2307 98-99 0.45566887539003176 2307 100-101 0.34017622389385593 2201 1 0.1832914447586056 2201 2 0.07778614067350631 2201 3 0.10651520864854902 2201 4 0.16876098357263913 2201 5 0.2169427658205194 2201 6 0.2383014768973979 2201 7 0.30796510187012416 2201 8 0.2529483170335851 2201 9 0.23090289772915895 2201 10-11 0.3327778575692335 2201 12-13 0.3480622350803344 2201 14-15 0.5256556657177711 2201 16-17 0.5748917817760315 2201 18-19 0.6739471301336479 2201 20-21 0.5418114840379857 2201 22-23 0.47581719126413446 2201 24-25 0.5958692695910628 2201 26-27 0.5475948302048934 2201 28-29 0.6618094699418648 2201 30-31 0.6370089659688674 2201 32-33 0.4896471160937992 2201 34-35 0.4768189382672787 2201 36-37 0.38654889064608255 2201 38-39 0.6001142376840676 2201 40-41 0.613351340686954 2201 42-43 0.5714768040247762 2201 44-45 0.5590967333496124 2201 46-47 0.5737598115944635 2201 48-49 0.5384776472162542 2201 50-51 0.5239196495526812 2201 52-53 0.5504566463324849 2201 54-55 0.6302576894301666 2201 56-57 0.6821087612103369 2201 58-59 0.6217869410131698 2201 60-61 0.664661929228842 2201 62-63 0.6510414142652792 2201 64-65 0.6252117827597061 2201 66-67 0.6267432968736557 2201 68-69 0.6237135712379853 2201 70-71 0.6845720870241649 2201 72-73 0.6210366069680191 2201 74-75 0.6446432136554563 2201 76-77 0.7035220580925454 2201 78-79 0.697792321553969 2201 80-81 0.653564434674351 2201 82-83 0.6169728271701054 2201 84-85 0.5987876626977346 2201 86-87 0.5612303513588905 2201 88-89 0.6149270507773821 2201 90-91 0.625955486552698 2201 92-93 0.6032146051107468 2201 94-95 0.6282195532825234 2201 96-97 0.7706412625274588 2201 98-99 0.7286811684958643 2201 100-101 0.4985805380899073 1101 1 0.2913576799092539 1101 2 0.15840155939287826 1101 3 0.268221324642699 1101 4 0.35258310178955554 1101 5 0.32228664059245915 1101 6 0.4225517339022673 1101 7 0.4415111833730734 1101 8 0.36585235490394297 1101 9 0.4806509380007 1101 10-11 0.4935394447543331 1101 12-13 0.4657930539684685 1101 14-15 0.6387395436120116 1101 16-17 0.684414175209497 1101 18-19 0.6907325094993411 1101 20-21 0.5674102147104918 1101 22-23 0.5338495049279075 1101 24-25 0.5639802000716045 1101 26-27 0.6205574362609596 1101 28-29 0.6133964889435433 1101 30-31 0.5867342746269699 1101 32-33 0.5829291610841807 1101 34-35 0.569479343744824 1101 36-37 0.6069587127107923 1101 38-39 0.5926642398599427 1101 40-41 0.7125008119893579 1101 42-43 0.6159883353898721 1101 44-45 0.6317999671945813 1101 46-47 0.49868168557977555 1101 48-49 0.5226445584215398 1101 50-51 0.6252454154096156 1101 52-53 0.5932607194360742 1101 54-55 0.8004881604620024 1101 56-57 0.7638372424627633 1101 58-59 0.7537862068539809 1101 60-61 0.6293602458423919 1101 62-63 0.6851492869220834 1101 64-65 0.727457834855926 1101 66-67 0.6727734945776263 1101 68-69 0.6475950638289447 1101 70-71 0.7913754835090927 1101 72-73 0.7231132198013484 1101 74-75 0.6434528698461364 1101 76-77 0.6454918659770357 1101 78-79 0.7564492500726416 1101 80-81 0.7097868606916933 1101 82-83 0.6807494397291869 1101 84-85 0.6829980190717926 1101 86-87 0.6668311145781196 1101 88-89 0.7669915061042758 1101 90-91 0.7195507445094229 1101 92-93 0.7554312427165151 1101 94-95 0.6247081839312685 1101 96-97 0.6730136398863173 1101 98-99 0.7198026960064716 1101 100-101 0.6957264709602917 2304 1 0.2687824685738356 2304 2 0.18240583079368022 2304 3 0.2164851742188354 2304 4 0.26283825363046276 2304 5 0.2663182756546547 2304 6 0.34907625095629413 2304 7 0.39351403415173536 2304 8 0.38872603990481736 2304 9 0.4174934622746278 2304 10-11 0.4263775069399287 2304 12-13 0.38442232045310476 2304 14-15 0.6105064185650804 2304 16-17 0.6790642457042964 2304 18-19 0.6528922126941836 2304 20-21 0.5594882917220403 2304 22-23 0.4813167573231212 2304 24-25 0.5682213292278462 2304 26-27 0.594177150491852 2304 28-29 0.6114609389470544 2304 30-31 0.5858284849958508 2304 32-33 0.6258762369504112 2304 34-35 0.540163375689751 2304 36-37 0.5888260442413369 2304 38-39 0.5733625376161484 2304 40-41 0.6707621468736278 2304 42-43 0.608822202449673 2304 44-45 0.635770224706178 2304 46-47 0.6520507172088799 2304 48-49 0.612317398199913 2304 50-51 0.42628771348044836 2304 52-53 0.5089673657309532 2304 54-55 0.6389151789789551 2304 56-57 0.6872345792729604 2304 58-59 0.6326334053561311 2304 60-61 0.6088345918139382 2304 62-63 0.6213937197398849 2304 64-65 0.7045965922167667 2304 66-67 0.5815713407619114 2304 68-69 0.5796915898595785 2304 70-71 0.6166403902092554 2304 72-73 0.5577930375517397 2304 74-75 0.6033128336870206 2304 76-77 0.6978674155444722 2304 78-79 0.7199019577760666 2304 80-81 0.6488316591528474 2304 82-83 0.6737250114640538 2304 84-85 0.6278161687114512 2304 86-87 0.6279037558030929 2304 88-89 0.5883664585813762 2304 90-91 0.7596239208513005 2304 92-93 0.7363061648027802 2304 94-95 0.7811820853624987 2304 96-97 0.7850794175518025 2304 98-99 0.7254295038136966 2304 100-101 0.6661485935747571 2202 1 0.09769832528398936 2202 2 0.03700230540865235 2202 3 0.008029029132451626 2202 4 -0.004388940553340603 2202 5 0.005060827852872762 2202 6 0.16003317594616817 2202 7 0.04552268942111937 2202 8 0.13739002650596888 2202 9 0.05133968079362461 2202 10-11 0.12920274882063154 2202 12-13 0.1414172472085511 2202 14-15 0.1238032531525235 2202 16-17 0.32676051035375764 2202 18-19 0.33848251666849194 2202 20-21 0.31785790623952437 2202 22-23 0.45622455171179865 2202 24-25 0.3373486559399268 2202 26-27 0.36864417218976087 2202 28-29 0.34102129462730346 2202 30-31 0.2573509893599848 2202 32-33 0.19285620725876385 2202 34-35 0.2619633265724346 2202 36-37 0.23055875748686105 2202 38-39 0.25629771823294334 2202 40-41 0.29290094093263974 2202 42-43 0.2936141507481551 2202 44-45 0.36182581578452755 2202 46-47 0.25960726297759606 2202 48-49 0.34586375765466926 2202 50-51 0.260964966184666 2202 52-53 0.3283218932643166 2202 54-55 0.4259120969773562 2202 56-57 0.4540306472318676 2202 58-59 0.4658302131724241 2202 60-61 0.5163110232410659 2202 62-63 0.4720214480192979 2202 64-65 0.4871607378961045 2202 66-67 0.5028733260311427 2202 68-69 0.47688249917173664 2202 70-71 0.4457918552109419 2202 72-73 0.6065821184603664 2202 74-75 0.5467248284920814 2202 76-77 0.5288242741595823 2202 78-79 0.49732347514744646 2202 80-81 0.4247331280264035 2202 82-83 0.5109573386296908 2202 84-85 0.5843298120444338 2202 86-87 0.48046015775981843 2202 88-89 0.3352254719426142 2202 90-91 0.44221523593451906 2202 92-93 0.40443158655909883 2202 94-95 0.4572153745197376 2202 96-97 0.5878026192934769 2202 98-99 0.5604130318094676 2202 100-101 0.5065512007755366 1102 1 -2.848875523469019E-4 1102 2 0.04901104563418812 1102 3 0.06997181594077162 1102 4 0.014501360587686918 1102 5 0.028917452371473473 1102 6 0.03435404211643345 1102 7 0.061897069593591425 1102 8 0.1528805687652337 1102 9 0.09840838789543227 1102 10-11 0.16005182296718345 1102 12-13 0.1494965925862033 1102 14-15 0.12388974841899625 1102 16-17 0.17351013544430316 1102 18-19 0.20428124878910126 1102 20-21 0.1732993220036505 1102 22-23 0.20081218858325656 1102 24-25 0.24321289833705606 1102 26-27 0.27322229188992964 1102 28-29 0.13763909571344612 1102 30-31 0.1991880477671728 1102 32-33 0.2194156587251186 1102 34-35 0.1682435414169774 1102 36-37 0.1019970762358966 1102 38-39 0.2230610061381455 1102 40-41 0.1974398406101443 1102 42-43 0.320690006082188 1102 44-45 0.2681735371094902 1102 46-47 0.20948622758556468 1102 48-49 0.17545058486251008 1102 50-51 0.1519480261995838 1102 52-53 0.28911371528906926 1102 54-55 0.3689953125885026 1102 56-57 0.4674412030089954 1102 58-59 0.3128859573733891 1102 60-61 0.3382730141935397 1102 62-63 0.39596957902163155 1102 64-65 0.2916463157370828 1102 66-67 0.3260757382074857 1102 68-69 0.3578649503858813 1102 70-71 0.3251215986904583 1102 72-73 0.3635527009584649 1102 74-75 0.3978534705805927 1102 76-77 0.3929443106213668 1102 78-79 0.3992684900837702 1102 80-81 0.4465060180036744 1102 82-83 0.42882909950931136 1102 84-85 0.5298966449095737 1102 86-87 0.4729770644058142 1102 88-89 0.40610655161558284 1102 90-91 0.3059241247985618 1102 92-93 0.35359307738380963 1102 94-95 0.3314296591394843 1102 96-97 0.5092488276035674 1102 98-99 0.3885918105054138 1102 100-101 0.4849875394725309 2305 1 0.04211642263770443 2305 2 -0.021846463064296273 2305 3 0.03238518694381298 2305 4 -0.07898725280806218 2305 5 -0.043236070855755315 2305 6 0.1174765145598613 2305 7 -0.021356352139221713 2305 8 0.07793226003862941 2305 9 0.013695584552337436 2305 10-11 0.10548878633798608 2305 12-13 0.01142991302213403 2305 14-15 -0.0036169805967247726 2305 16-17 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-0.4279674431179039 1213 92-93 -0.36670128336698227 1213 94-95 -0.24400448870130376 1213 96-97 -0.37642626657775224 1213 98-99 -0.3383978347414889 1213 100-101 -0.2622630305996161 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1939.0 3 445.0 4 479.0 5 759.0 6 2016.0 7 4044.0 8 14466.0 9 34920.0 10 43423.0 11 35609.0 12 24600.0 13 19487.0 14 17541.0 15 17562.0 16 17909.0 17 18401.0 18 19068.0 19 20903.0 20 22577.0 21 24955.0 22 27789.0 23 30930.0 24 34589.0 25 39908.0 26 46151.0 27 55248.0 28 66609.0 29 80816.0 30 99018.0 31 128231.0 32 174992.0 33 247816.0 34 369692.0 35 611466.0 36 1099889.0 37 1495690.0 38 307103.0 39 76.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.91185586509012 24.0091640126417 26.38188521189491 13.697094910373272 2 31.46584734066982 22.375401002843123 34.2837865687807 11.874965087706354 3 38.49594682997272 18.10333982430164 27.864095287618007 15.536618058107637 4 29.799576621598934 16.878073157515864 36.54142119365874 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28.20627647752269 30.14004547556246 18.757449214696166 26-27 22.208301018715833 28.58850228011941 30.14281112116013 19.06038558000463 28-29 22.02505826922789 28.36638726213382 29.730993931386525 19.877560537251764 30-31 22.379453391456124 27.907603346961444 29.530039464636538 20.18290379694589 32-33 21.06026175965621 27.983270548736233 30.77986716161317 20.176600529994392 34-35 20.557293682099544 28.11859107255582 32.21702189180588 19.107093353538758 36-37 20.796175848586905 29.02928287044606 30.70938764579663 19.465153635170402 38-39 20.78799898484783 28.826016987095944 29.276755621013802 21.109228407042423 40-41 21.572817568945098 27.95965498625947 29.943909687400023 20.523617757395414 42-43 21.834392934317467 28.204151751981293 31.104669229311526 18.85678608438971 44-45 20.86213003333197 29.355229753690086 31.24202742893728 18.54061278404067 46-47 21.79237925780194 29.087780561055766 30.469450710505985 18.650389470636313 48-49 21.36588875809412 29.400950198756714 29.94442410189112 19.288736941258044 50-51 20.49563165643659 29.488536134999432 29.867411338916085 20.148420869647897 52-53 21.920546338423755 28.94801751484134 29.336767421295466 19.794668725439436 54-55 22.761245915814357 28.781222218351648 29.05881561360252 19.39871625223148 56-57 22.950404713390533 28.508289170113247 28.392760400738986 20.14854571575723 58-59 22.142278313283942 28.23074465923544 29.12743899360577 20.499538033874842 60-61 20.55604796168195 28.298949560808616 29.389130045438662 21.755872432070774 62-63 21.810479135322026 28.310962363534543 29.10320857808787 20.77534992305557 64-65 20.978383900522363 28.731966548720656 29.66615313340518 20.623496417351802 66-67 21.133471523711126 29.05058935740711 28.682696597822112 21.133242521059657 68-69 21.545801677853728 28.490776079148787 30.083127449271323 19.880294793726165 70-71 20.912352879277364 30.68510228962103 29.112065280201122 19.290479550900486 72-73 20.41698635296057 31.86316272363851 28.252493839271892 19.46735708412903 74-75 21.55539753078128 31.258911551049202 27.601905786409738 19.583785131759782 76-77 22.043392818795013 30.012515673211855 28.254139430838816 19.68995207715432 78-79 21.893620569511324 29.907129462353772 28.975418844750962 19.223831123383945 80-81 20.647223778005287 30.76754120227735 28.818818751961956 19.766416267755407 82-83 19.78339704382796 30.416868207655106 29.047032126810674 20.75270262170626 84-85 19.580992613234645 29.47428831755104 29.87362425586739 21.071094813346928 86-87 19.842756026087077 29.452399483297597 30.546197575201724 20.158646915413602 88-89 20.032608046244903 30.060655967073945 29.960567990439195 19.946167996241954 90-91 20.38408253221911 29.696047179152668 29.41734177757519 20.502528511053033 92-93 19.744304467931734 30.604414621683716 29.205407402121487 20.445873508263055 94-95 19.571455569868863 31.030585798419725 29.361920006960446 20.03603862475097 96-97 20.3618344537201 31.378204522725543 28.5166529564071 19.74330806714726 98-99 20.775101579717532 30.443406512973326 28.1608044766266 20.620687430682544 100-101 20.49413839810908 30.35510888763317 28.210319652683424 20.94043306157433 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3564.0 1 2397.0 2 1025.5 3 1032.5 4 1173.5 5 1155.0 6 1465.5 7 2013.0 8 2507.0 9 2788.5 10 2880.5 11 2904.5 12 2808.0 13 2543.0 14 2228.5 15 1970.5 16 1794.5 17 1684.5 18 1713.0 19 1857.0 20 2102.5 21 2450.0 22 2964.5 23 3694.5 24 4596.0 25 5732.5 26 7343.0 27 9545.0 28 12093.5 29 14811.5 30 18268.0 31 23551.5 32 34868.0 33 55415.0 34 84581.5 35 125370.5 36 176531.5 37 260799.5 38 367612.5 39 428275.0 40 419815.0 41 361451.5 42 314718.5 43 310137.0 44 306774.0 45 298377.5 46 297495.5 47 268963.0 48 212801.5 49 170412.5 50 141664.5 51 118378.5 52 97034.5 53 71820.0 54 49608.0 55 36337.5 56 26760.0 57 19519.0 58 14373.0 59 10409.5 60 7752.5 61 5853.5 62 4277.0 63 3075.0 64 2294.0 65 1660.0 66 1188.5 67 921.0 68 702.5 69 493.5 70 354.0 71 278.0 72 227.0 73 176.0 74 131.0 75 96.5 76 78.0 77 75.5 78 55.0 79 46.0 80 39.0 81 25.5 82 25.0 83 19.0 84 17.0 85 16.0 86 10.5 87 8.5 88 8.5 89 7.5 90 7.5 91 4.0 92 3.0 93 2.5 94 2.0 95 1.5 96 1.5 97 1.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23305553843590288 2 0.22500930167795422 3 0.20642496760581278 4 0.1954113243839398 5 0.19864503655616503 6 0.1267424953149217 7 0.10214726096970277 8 0.09636462273231179 9 0.09029665695031268 10-11 0.07067563279942843 12-13 0.09976953143130188 14-15 0.10186193342509467 16-17 0.09134285794720907 18-19 0.08551266511905006 20-21 0.07949225392781897 22-23 0.15973587038977263 24-25 0.627558912529227 26-27 0.3595792826332917 28-29 0.1846354541159069 30-31 0.3157910154541007 32-33 0.35323550022483813 34-35 0.3347557862523863 36-37 0.3442476825696827 38-39 0.35176130791102955 40-41 0.35380615531405435 42-43 0.3745114241344494 44-45 0.35098141262243404 46-47 0.3744733804618349 48-49 0.3624420689975264 50-51 0.36855758937029354 52-53 0.36225185063445436 54-55 0.34540801458442233 56-57 0.35369202429621105 58-59 0.3442096388970683 60-61 0.322886160396689 62-63 0.3104553903699291 64-65 0.3100559318074777 66-67 0.3234092608951372 68-69 0.3216497410367205 70-71 0.30700292708017096 72-73 0.29768222728963945 74-75 0.3048249268229957 76-77 0.30601379159219616 78-79 0.3095233203908759 80-81 0.3197760901604606 82-83 0.348365910130193 84-85 0.3336905634191827 86-87 0.34474225031367006 88-89 0.3466729666988516 90-91 0.32669052765813045 92-93 0.3058330841472777 94-95 0.3057284640475881 96-97 0.31414562661352724 98-99 0.33545008327759934 100-101 0.3419460403765106 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5257116.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.73688744843865 #Duplication Level Percentage of deduplicated Percentage of total 1 86.77243310352955 31.87749108551538 2 5.816978879023274 4.273953967372457 3 1.9791907032296754 2.1812791831063416 4 1.2333127880558716 1.8123229233411455 5 0.6583658284259917 1.2093155669391866 6 0.41426174830696244 0.9131212333047781 7 0.34300682653518705 0.8820702226328503 8 0.3221199988128616 0.9466948913023431 9 0.23184754094988463 0.7665621315365866 >10 1.7154224755663683 12.341957072294855 >50 0.23175902812989124 5.9639744356475415 >100 0.23161280309860255 17.936783686044784 >500 0.03562940864285044 8.978444519198835 >1k 0.01342997857371486 8.058886280101618 >5k 5.240756704299551E-4 1.3305457042097992 >10k+ 1.0481344876126587E-4 0.5265970974516002 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 14260 0.271251385740775 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 13092 0.24903388093395692 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 9233 0.17562861462444426 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 9089 0.17288947019620643 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT 8814 0.16765846521172445 Illumina Single End PCR Primer 1 (96% over 32bp) TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 6761 0.12860663527302804 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6518 0.12398432905037667 No Hit GTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGT 6040 0.11489189129553162 No Hit TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC 5771 0.10977501732889287 No Hit TGTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAG 5723 0.10886196918614692 No Hit TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT 5667 0.10779674635294331 No Hit GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT 5493 0.10448694683548927 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0013315285415045055 0.0 0.0 2 0.0015597905771909922 0.0 0.0 3 0.0017500089402630644 0.0 0.0 4 0.0018641399581063077 0.0 0.0 5 0.002149467502714416 0.0 1.9021836307207222E-5 6 0.00239675137470811 0.0 1.9021836307207222E-5 7 0.0028722972823882905 0.0 1.9021836307207222E-5 8 0.0033668650263756783 0.0 1.9021836307207222E-5 9 0.0037473017525198227 0.0 1.9021836307207222E-5 10-11 0.0064198697536824376 0.0 1.9021836307207222E-5 12-13 0.009254123363456313 0.0 1.9021836307207222E-5 14-15 0.012659032062446406 0.0 1.9021836307207222E-5 16-17 0.01714818543094731 0.0 1.9021836307207222E-5 18-19 0.02270256163265182 0.0 1.9021836307207222E-5 20-21 0.027458020709453624 0.0 1.9021836307207222E-5 22-23 0.032441741821941913 0.0 1.9021836307207222E-5 24-25 0.038224380059332916 0.0 1.9021836307207222E-5 26-27 0.045576319792068505 0.0 1.9021836307207222E-5 28-29 0.05280461758880725 0.0 1.9021836307207222E-5 30-31 0.08106155542316357 0.0 1.9021836307207222E-5 32-33 0.09081975744876088 0.0 1.9021836307207222E-5 34-35 0.10013094632113881 0.0 1.9021836307207222E-5 36-37 0.110107899464269 9.510918153603611E-6 1.9021836307207222E-5 38-39 0.1206364858603082 1.9021836307207222E-5 1.9021836307207222E-5 40-41 0.13176426010002443 1.9021836307207222E-5 1.9021836307207222E-5 42-43 0.14338660208372803 1.9021836307207222E-5 1.9021836307207222E-5 44-45 0.15651166913570103 1.9021836307207222E-5 1.9021836307207222E-5 46-47 0.17018836944058302 1.9021836307207222E-5 1.9021836307207222E-5 48-49 0.18539632756819518 1.9021836307207222E-5 1.9021836307207222E-5 50-51 0.2012224953757916 1.9021836307207222E-5 1.9021836307207222E-5 52-53 0.21769540561783304 1.9021836307207222E-5 1.9021836307207222E-5 54-55 0.23473897094909071 1.9021836307207222E-5 3.8043672614414444E-5 56-57 0.2537037417473763 1.9021836307207222E-5 3.8043672614414444E-5 58-59 0.27348645150687184 1.9021836307207222E-5 3.8043672614414444E-5 60-61 0.2942868295088029 1.9021836307207222E-5 3.8043672614414444E-5 62-63 0.3163046050343953 1.9021836307207222E-5 3.8043672614414444E-5 64-65 0.3394636907384201 1.9021836307207222E-5 3.8043672614414444E-5 66-67 0.3643157198737863 1.9021836307207222E-5 3.8043672614414444E-5 68-69 0.39076558325895794 1.9021836307207222E-5 3.8043672614414444E-5 70-71 0.4192032285382328 1.9021836307207222E-5 3.8043672614414444E-5 72-73 0.4482590834974918 1.9021836307207222E-5 3.8043672614414444E-5 74-75 0.4786654888345625 1.9021836307207222E-5 3.8043672614414444E-5 76-77 0.511668374827567 1.9021836307207222E-5 3.8043672614414444E-5 78-79 0.5462881169066842 1.9021836307207222E-5 3.8043672614414444E-5 80-81 0.5847502699198572 1.9021836307207222E-5 3.8043672614414444E-5 82-83 0.6267029298954028 1.9021836307207222E-5 3.8043672614414444E-5 84-85 0.6729450139582235 1.9021836307207222E-5 3.8043672614414444E-5 86-87 0.7207659104345424 1.9021836307207222E-5 3.8043672614414444E-5 88-89 0.7744835761660956 1.9021836307207222E-5 4.7554590768018054E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 1650 0.0 76.662796 9 CGGGAGA 2170 0.0 76.50377 4 AGAGCGG 1745 0.0 72.489174 8 GAGAGCG 2065 0.0 71.58003 7 CGGAAAG 975 0.0 69.56278 5 TCGGGAA 890 0.0 65.55568 3 AAGCGGC 780 0.0 64.382614 9 ATTCGGA 320 0.0 62.263252 2 GAACCGG 350 0.0 61.00479 1 ACCGGAA 435 0.0 59.974777 3 TCGGGAG 2750 0.0 59.508858 3 AAAGCGG 985 0.0 54.830975 8 TCGGAGA 465 0.0 54.062664 4 AAGCGTC 500 0.0 53.06099 9 CGGAGAG 1040 0.0 52.44572 5 TTCGGAG 345 0.0 52.246803 3 GATCCGG 530 0.0 51.029163 1 AACCGGA 415 0.0 50.29642 2 GGATCGG 795 0.0 49.53708 1 AGAGCGT 8385 0.0 45.200607 8 >>END_MODULE