FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n02_34298-1.342000000357ce.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n02_34298-1.342000000357ce.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5257116
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC142600.271251385740775No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG130920.24903388093395692No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA92330.17562861462444426No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA90890.17288947019620643No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT88140.16765846521172445Illumina Single End PCR Primer 1 (96% over 32bp)
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA67610.12860663527302804No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA65180.12398432905037667No Hit
GTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGT60400.11489189129553162No Hit
TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC57710.10977501732889287No Hit
TGTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAG57230.10886196918614692No Hit
TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT56670.10779674635294331No Hit
GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT54930.10448694683548927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC16500.076.6627969
CGGGAGA21700.076.503774
AGAGCGG17450.072.4891748
GAGAGCG20650.071.580037
CGGAAAG9750.069.562785
TCGGGAA8900.065.555683
AAGCGGC7800.064.3826149
ATTCGGA3200.062.2632522
GAACCGG3500.061.004791
ACCGGAA4350.059.9747773
TCGGGAG27500.059.5088583
AAAGCGG9850.054.8309758
TCGGAGA4650.054.0626644
AAGCGTC5000.053.060999
CGGAGAG10400.052.445725
TTCGGAG3450.052.2468033
GATCCGG5300.051.0291631
AACCGGA4150.050.296422
GGATCGG7950.049.537081
AGAGCGT83850.045.2006078