##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n02_34274-2.3420000003583a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4522654 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.24458758065508 33.0 33.0 33.0 33.0 33.0 2 32.24484362500426 33.0 33.0 33.0 33.0 33.0 3 32.22537762119322 33.0 33.0 33.0 33.0 33.0 4 36.0166844512094 37.0 37.0 37.0 33.0 37.0 5 36.01091792562509 37.0 37.0 37.0 33.0 37.0 6 35.978885185556976 37.0 37.0 37.0 33.0 37.0 7 35.925742274337146 37.0 37.0 37.0 33.0 37.0 8 35.92379916747998 37.0 37.0 37.0 33.0 37.0 9 35.912250196455446 37.0 37.0 37.0 33.0 37.0 10-11 35.8122921850754 37.0 37.0 37.0 33.0 37.0 12-13 35.81462123788377 37.0 37.0 37.0 33.0 37.0 14-15 37.648631201944696 40.0 37.0 40.0 33.0 40.0 16-17 37.59929468405056 40.0 37.0 40.0 33.0 40.0 18-19 37.57195631149321 40.0 37.0 40.0 33.0 40.0 20-21 37.56832083550941 40.0 37.0 40.0 33.0 40.0 22-23 37.541842687943856 40.0 37.0 40.0 33.0 40.0 24-25 37.26301370832259 40.0 37.0 40.0 33.0 40.0 26-27 37.21892996899608 40.0 37.0 40.0 33.0 40.0 28-29 37.231607370362624 40.0 37.0 40.0 33.0 40.0 30-31 37.209711045770916 40.0 37.0 40.0 33.0 40.0 32-33 37.14979136144397 40.0 37.0 40.0 33.0 40.0 34-35 37.08302945571339 40.0 37.0 40.0 33.0 40.0 36-37 37.00031032663564 40.0 37.0 40.0 33.0 40.0 38-39 36.93024162361304 40.0 37.0 40.0 33.0 40.0 40-41 36.796058575340936 40.0 37.0 40.0 33.0 40.0 42-43 36.731121925311996 40.0 37.0 40.0 33.0 40.0 44-45 36.626382982204696 40.0 37.0 40.0 33.0 40.0 46-47 36.52975188904568 40.0 37.0 40.0 33.0 40.0 48-49 36.39446628019742 37.0 37.0 40.0 33.0 40.0 50-51 35.260278367524904 37.0 35.0 38.5 30.0 38.5 52-53 35.695473387970864 37.0 37.0 38.5 33.0 40.0 54-55 36.45599497551659 37.0 37.0 40.0 33.0 40.0 56-57 36.50308425097299 37.0 37.0 40.0 33.0 40.0 58-59 36.346853639478056 37.0 37.0 40.0 33.0 40.0 60-61 36.100532784511046 37.0 37.0 40.0 33.0 40.0 62-63 35.81667644705962 37.0 37.0 40.0 33.0 40.0 64-65 35.574232629778884 37.0 37.0 40.0 27.0 40.0 66-67 35.34479677198388 37.0 37.0 40.0 27.0 40.0 68-69 35.148187104297605 37.0 33.0 37.0 27.0 40.0 70-71 34.798108809561825 37.0 33.0 37.0 27.0 40.0 72-73 34.515359012650535 37.0 33.0 37.0 27.0 40.0 74-75 34.292421109375155 37.0 33.0 37.0 27.0 38.5 76-77 34.03017652909111 37.0 33.0 37.0 27.0 37.0 78-79 33.75597193594734 37.0 33.0 37.0 27.0 37.0 80-81 33.537672459577934 37.0 33.0 37.0 27.0 37.0 82-83 33.29997861874908 37.0 33.0 37.0 27.0 37.0 84-85 33.11487889632946 37.0 33.0 37.0 27.0 37.0 86-87 32.95598679447952 37.0 33.0 37.0 27.0 37.0 88-89 32.797571956643154 37.0 33.0 37.0 27.0 37.0 90-91 32.631766989029 35.0 33.0 37.0 27.0 37.0 92-93 32.48746112349076 33.0 33.0 37.0 24.5 37.0 94-95 32.36212763567587 33.0 33.0 37.0 22.0 37.0 96-97 32.156163394325546 33.0 33.0 37.0 22.0 37.0 98-99 32.03294956899201 33.0 33.0 37.0 22.0 37.0 100-101 30.765780446613867 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.15273589065098037 2306 2 0.18216895196157168 2306 3 0.1376166589689518 2306 4 0.2545285444065044 2306 5 0.28731790545229785 2306 6 0.2830016856974922 2306 7 0.36889485664605104 2306 8 0.3549992950957943 2306 9 0.27042733360249116 2306 10-11 0.33467187381491925 2306 12-13 0.2953822907804238 2306 14-15 0.5116878319288034 2306 16-17 0.5931655912161702 2306 18-19 0.5731448674017301 2306 20-21 0.5493377682819727 2306 22-23 0.5120432558876118 2306 24-25 0.515472416420522 2306 26-27 0.5429732564951308 2306 28-29 0.5624586042682935 2306 30-31 0.5020151223906524 2306 32-33 0.4776760325735765 2306 34-35 0.4502648485262881 2306 36-37 0.41402407411193565 2306 38-39 0.48891734602344883 2306 40-41 0.5808087204984105 2306 42-43 0.4248110414668105 2306 44-45 0.5602806463457242 2306 46-47 0.5488136611341972 2306 48-49 0.5954413689387223 2306 50-51 0.29606644980805896 2306 52-53 0.4081875270641788 2306 54-55 0.5282880394931482 2306 56-57 0.5377961496367334 2306 58-59 0.4476295283230911 2306 60-61 0.40809370348065954 2306 62-63 0.4819177196249953 2306 64-65 0.4516920435638525 2306 66-67 0.5171343651732627 2306 68-69 0.5001859505502892 2306 70-71 0.4999885820343195 2306 72-73 0.3923622167453473 2306 74-75 0.4382861001527445 2306 76-77 0.4007896586954516 2306 78-79 0.3188006127755898 2306 80-81 0.39640048046845067 2306 82-83 0.4704117028424619 2306 84-85 0.5729281329362621 2306 86-87 0.5889654151868484 2306 88-89 0.5839790406008092 2306 90-91 0.590858283586833 2306 92-93 0.5748005153744629 2306 94-95 0.4788864087405713 2306 96-97 0.6379377188067465 2306 98-99 0.6940934776028982 2306 100-101 0.4538356329517903 2307 1 0.2946994594646881 2307 2 0.20846705234514928 2307 3 0.2438423694052787 2307 4 0.3193514237902946 2307 5 0.3527403081336544 2307 6 0.33772161329782335 2307 7 0.45918787699921637 2307 8 0.3948808388184659 2307 9 0.3341717028952331 2307 10-11 0.4122543684150841 2307 12-13 0.3750653040763794 2307 14-15 0.6170013775100713 2307 16-17 0.5784380231567923 2307 18-19 0.6307034782768355 2307 20-21 0.602203961097608 2307 22-23 0.5617711252882529 2307 24-25 0.5838785836124316 2307 26-27 0.6778978336938977 2307 28-29 0.5665795675104945 2307 30-31 0.5541196994727713 2307 32-33 0.5503501883806194 2307 34-35 0.4810358009785247 2307 36-37 0.4418807649571832 2307 38-39 0.39502657834200505 2307 40-41 0.5178139375904891 2307 42-43 0.5424882058261105 2307 44-45 0.5375348490842313 2307 46-47 0.4942480494211665 2307 48-49 0.5288478666339458 2307 50-51 0.4141119066511223 2307 52-53 0.5088598096950605 2307 54-55 0.44491080106222114 2307 56-57 0.3899699137001349 2307 58-59 0.4890996751248835 2307 60-61 0.4406356433796077 2307 62-63 0.5601934206322525 2307 64-65 0.5886929592524552 2307 66-67 0.5367186308823833 2307 68-69 0.43323023119037884 2307 70-71 0.5074495729221482 2307 72-73 0.5614608369911949 2307 74-75 0.4480637734871493 2307 76-77 0.42915461990019566 2307 78-79 0.5529821407507498 2307 80-81 0.5305921179568642 2307 82-83 0.5467094873037581 2307 84-85 0.4329478270457159 2307 86-87 0.4494103893006596 2307 88-89 0.3558796242307807 2307 90-91 0.4726831770967195 2307 92-93 0.5132878940360115 2307 94-95 0.5002726465824239 2307 96-97 0.5800425291853273 2307 98-99 0.597358437252332 2307 100-101 0.4714832950296426 2201 1 0.24814844640334144 2201 2 0.21805034680297553 2201 3 0.2558456126547668 2201 4 0.30616230551984813 2201 5 0.35970848498652686 2201 6 0.35080968964959425 2201 7 0.4175625284613673 2201 8 0.3750425636855539 2201 9 0.37795102982188666 2201 10-11 0.4174896608813796 2201 12-13 0.4103748102321916 2201 14-15 0.6031268977882291 2201 16-17 0.5892346463131659 2201 18-19 0.6021902538609893 2201 20-21 0.585381358918319 2201 22-23 0.535793856933303 2201 24-25 0.5121707130352604 2201 26-27 0.6145010995668727 2201 28-29 0.656441492025678 2201 30-31 0.6273885077189547 2201 32-33 0.63952370676963 2201 34-35 0.5024781036691479 2201 36-37 0.5849358411628174 2201 38-39 0.5267941678926036 2201 40-41 0.5917800795685224 2201 42-43 0.5510558174222169 2201 44-45 0.6044683083879718 2201 46-47 0.49258431527514546 2201 48-49 0.47625900547854627 2201 50-51 0.41506478664511093 2201 52-53 0.5389775261105498 2201 54-55 0.6232203728591585 2201 56-57 0.5656475963785539 2201 58-59 0.6122569760279291 2201 60-61 0.5178504533382267 2201 62-63 0.5977953088672976 2201 64-65 0.6202832661639306 2201 66-67 0.6423791663653233 2201 68-69 0.5627362982467119 2201 70-71 0.5621745056503187 2201 72-73 0.6304630753948075 2201 74-75 0.4768122635348817 2201 76-77 0.47002984314193696 2201 78-79 0.5462427789476081 2201 80-81 0.6573932237001756 2201 82-83 0.649257384682322 2201 84-85 0.6660196417379396 2201 86-87 0.5456186029455878 2201 88-89 0.5888552994467631 2201 90-91 0.6066444290174431 2201 92-93 0.6393744300919764 2201 94-95 0.5955799461230953 2201 96-97 0.6278933603505763 2201 98-99 0.6815360055748343 2201 100-101 0.5373941048429494 1101 1 0.312118505801422 1101 2 0.2774303266866909 1101 3 0.26278614041837756 1101 4 0.2793798966697736 1101 5 0.3544127432974662 1101 6 0.3840176639233164 1101 7 0.3861434569311868 1101 8 0.34579561422328453 1101 9 0.40172846055103406 1101 10-11 0.4215810184758695 1101 12-13 0.4531408403684054 1101 14-15 0.6259272193212482 1101 16-17 0.6290091637748034 1101 18-19 0.5966704671609264 1101 20-21 0.5990971897795774 1101 22-23 0.5936242186943517 1101 24-25 0.5149747102712112 1101 26-27 0.5948530572198649 1101 28-29 0.48386837715404596 1101 30-31 0.552031457306775 1101 32-33 0.4964562053059467 1101 34-35 0.5620809912795295 1101 36-37 0.5231742514394995 1101 38-39 0.5430112509038949 1101 40-41 0.5613923429508318 1101 42-43 0.5687002832374901 1101 44-45 0.6301246237960427 1101 46-47 0.6348046763947579 1101 48-49 0.6380573977224486 1101 50-51 0.5356447197637024 1101 52-53 0.5671405071420068 1101 54-55 0.6476268127998566 1101 56-57 0.6008742048283935 1101 58-59 0.6617081908522877 1101 60-61 0.6536165439779751 1101 62-63 0.6974467084776208 1101 64-65 0.6108439882757821 1101 66-67 0.6098521524508769 1101 68-69 0.6989310540922347 1101 70-71 0.6513706230526353 1101 72-73 0.6747442866844935 1101 74-75 0.5647964907661134 1101 76-77 0.5809212056494957 1101 78-79 0.5900822235161414 1101 80-81 0.6597803834872522 1101 82-83 0.6624349616207397 1101 84-85 0.6903616658947627 1101 86-87 0.5967385323065884 1101 88-89 0.61401889779615 1101 90-91 0.653221726365885 1101 92-93 0.6497202600570517 1101 94-95 0.7181799691417297 1101 96-97 0.7168797625243357 1101 98-99 0.5487141165819693 1101 100-101 0.6495614548658537 2304 1 0.10807023911812763 2304 2 0.06362251384665285 2304 3 0.05277469770771148 2304 4 0.09539283671963261 2304 5 0.15917689172813 2304 6 0.2364862295045853 2304 7 0.2393290605063001 2304 8 0.233315213633432 2304 9 0.18577837075611114 2304 10-11 0.21827254306531074 2304 12-13 0.2771865137560283 2304 14-15 0.36750134153402314 2304 16-17 0.38037029299814407 2304 18-19 0.4029749010771084 2304 20-21 0.419612677494662 2304 22-23 0.34953220459970424 2304 24-25 0.27343572969243723 2304 26-27 0.3832230759138966 2304 28-29 0.3901046338225953 2304 30-31 0.31755759694252816 2304 32-33 0.27553041526957145 2304 34-35 0.2913352659846993 2304 36-37 0.2575608977837547 2304 38-39 0.3320866212434481 2304 40-41 0.38658703627503854 2304 42-43 0.37876495458063175 2304 44-45 0.379377626630081 2304 46-47 0.3380569086731171 2304 48-49 0.3918649380186707 2304 50-51 0.3662015061252717 2304 52-53 0.3953332649922032 2304 54-55 0.4879098619374389 2304 56-57 0.3960047553792876 2304 58-59 0.4180461352690443 2304 60-61 0.424789130695423 2304 62-63 0.455741600056804 2304 64-65 0.4903850327545243 2304 66-67 0.45427258183952546 2304 68-69 0.3577619897970905 2304 70-71 0.48143022342907216 2304 72-73 0.3704207094561056 2304 74-75 0.45498152736750797 2304 76-77 0.5237328419488634 2304 78-79 0.5566207808261154 2304 80-81 0.5246663755198924 2304 82-83 0.43592991068188525 2304 84-85 0.42719765522261355 2304 86-87 0.35588896368975753 2304 88-89 0.4077019516413145 2304 90-91 0.5023674460446657 2304 92-93 0.4765969616492498 2304 94-95 0.5984553127224359 2304 96-97 0.5752568714869426 2304 98-99 0.6109629581675335 2304 100-101 0.5093922134172288 2202 1 0.12055262076942341 2202 2 0.12539623018250268 2202 3 0.1614271270013461 2202 4 0.20817305582782808 2202 5 0.21921148775832222 2202 6 0.2284450549868282 2202 7 0.27439816520297455 2202 8 0.26354356781757815 2202 9 0.29806454806949745 2202 10-11 0.3572090573114224 2202 12-13 0.325595911761809 2202 14-15 0.3525451107540434 2202 16-17 0.4886120764409725 2202 18-19 0.3912067177763916 2202 20-21 0.4210185416382686 2202 22-23 0.41306622694151685 2202 24-25 0.46570422313433113 2202 26-27 0.4723247075768313 2202 28-29 0.43611884644257515 2202 30-31 0.4455835483333672 2202 32-33 0.3625939607219948 2202 34-35 0.43678172367736323 2202 36-37 0.32929942838434556 2202 38-39 0.36005604103191047 2202 40-41 0.4021288951418711 2202 42-43 0.4237853321665881 2202 44-45 0.45891624430317535 2202 46-47 0.3322433996222358 2202 48-49 0.39479995834529547 2202 50-51 0.46560109901037805 2202 52-53 0.4741482927156184 2202 54-55 0.46529591053496233 2202 56-57 0.4520824304561657 2202 58-59 0.48929062407484025 2202 60-61 0.47944645381302564 2202 62-63 0.4823208083668433 2202 64-65 0.47148625963226465 2202 66-67 0.5221796312174973 2202 68-69 0.5039480177185354 2202 70-71 0.5589132821491418 2202 72-73 0.5201066005077308 2202 74-75 0.5478270945327282 2202 76-77 0.48827653399759896 2202 78-79 0.5595271167337685 2202 80-81 0.44713555797026316 2202 82-83 0.47541117407765654 2202 84-85 0.6228477795703 2202 86-87 0.5457286003149591 2202 88-89 0.3553172499010202 2202 90-91 0.39173338933979096 2202 92-93 0.38845964336576344 2202 94-95 0.35851801074685596 2202 96-97 0.5613585060619997 2202 98-99 0.4761313371629683 2202 100-101 0.44924699308594285 1102 1 0.040602793956054484 1102 2 0.03110629538218035 1102 3 0.026281064450408564 1102 4 0.06589959922636268 1102 5 0.013725546438024594 1102 6 -0.019749856382361486 1102 7 -0.013602293232231943 1102 8 0.12014057780127274 1102 9 0.027960254059721024 1102 10-11 0.0975796894426324 1102 12-13 0.14109611551079126 1102 14-15 0.18542637603521683 1102 16-17 0.16167232246787222 1102 18-19 0.22713530375846602 1102 20-21 0.12806921146304973 1102 22-23 0.27862564523817923 1102 24-25 0.21146974625983717 1102 26-27 0.2748209509549895 1102 28-29 0.17932095269766535 1102 30-31 0.2805191763895962 1102 32-33 0.2563560583177562 1102 34-35 0.2410311633676656 1102 36-37 0.16311061675028782 1102 38-39 0.26330392713622786 1102 40-41 0.32769557199598864 1102 42-43 0.322317384428203 1102 44-45 0.359442808076615 1102 46-47 0.2905133797567103 1102 48-49 0.2750296216500985 1102 50-51 0.2865611387414262 1102 52-53 0.3242183314279288 1102 54-55 0.50940374468329 1102 56-57 0.45131264133636506 1102 58-59 0.4883027598735339 1102 60-61 0.5216915765717829 1102 62-63 0.46144959045729905 1102 64-65 0.3365557253005562 1102 66-67 0.4485463411808226 1102 68-69 0.4587281598334414 1102 70-71 0.45856022336755586 1102 72-73 0.5306471083520705 1102 74-75 0.4879650071244157 1102 76-77 0.5309775451019192 1102 78-79 0.4837729271453455 1102 80-81 0.5327473779580387 1102 82-83 0.5699723497092819 1102 84-85 0.5607377678547323 1102 86-87 0.4816848834241725 1102 88-89 0.5145900570526152 1102 90-91 0.43974811020368776 1102 92-93 0.4913134025344732 1102 94-95 0.5486298180003146 1102 96-97 0.6011554574678755 1102 98-99 0.5678005506918993 1102 100-101 0.5635549469367973 2305 1 0.032774367344764244 2305 2 0.0628676913697177 2305 3 0.06217270348535919 2305 4 0.08919117797394449 2305 5 0.0887034968907372 2305 6 0.1787982227950522 2305 7 0.12142299397009992 2305 8 0.1915437105116382 2305 9 0.12121309259004676 2305 10-11 0.13039862637534583 2305 12-13 0.15684591176180618 2305 14-15 0.14613101258052552 2305 16-17 0.10060372883823021 2305 18-19 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-0.6849565768918282 1213 62-63 -0.6670333067321721 1213 64-65 -0.5457820910867426 1213 66-67 -0.43728984809650484 1213 68-69 -0.4158545149900945 1213 70-71 -0.44641862939023014 1213 72-73 -0.4924068439170668 1213 74-75 -0.37675852251111763 1213 76-77 -0.4123343585648698 1213 78-79 -0.5291919058381254 1213 80-81 -0.49692550550215486 1213 82-83 -0.48080420192422224 1213 84-85 -0.5109240630157501 1213 86-87 -0.6009318413988538 1213 88-89 -0.5106486491442297 1213 90-91 -0.5536085778926534 1213 92-93 -0.4718457844984485 1213 94-95 -0.3895894562498441 1213 96-97 -0.5226015584712371 1213 98-99 -0.49583501125759 1213 100-101 -0.3768983614048622 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1775.0 3 309.0 4 313.0 5 519.0 6 1590.0 7 2026.0 8 5929.0 9 9469.0 10 8371.0 11 9865.0 12 8201.0 13 7352.0 14 7389.0 15 7555.0 16 8094.0 17 8704.0 18 9860.0 19 10857.0 20 12199.0 21 14181.0 22 16106.0 23 18765.0 24 21173.0 25 24878.0 26 30734.0 27 37671.0 28 45865.0 29 54945.0 30 66551.0 31 85146.0 32 117266.0 33 168623.0 34 256733.0 35 435171.0 36 861456.0 37 1580558.0 38 565902.0 39 553.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 33.11900305947582 32.781560873462176 16.093011256495068 18.006424810566937 2 30.62847890472843 25.701875539094488 29.927246068211577 13.742399487965503 3 31.922109052378655 23.733974078450654 25.374517042577803 18.969399826592888 4 28.06826734350286 23.308199379246798 29.433069187905126 19.190464089345213 5 29.377795158179502 21.71746926087626 30.54357206398302 18.361163516961216 6 27.441870248105882 22.042987898029942 31.959119872825255 18.55602198103892 7 31.462685694984106 22.883629446287294 25.254141545430276 20.39954331329832 8 27.50788066802744 27.155890038508034 25.12457896360749 20.21165032985703 9 26.062032845865584 24.715092968265168 28.516177266043368 20.70669691982588 10-11 22.805395821750903 29.091611140153624 30.824713239788792 17.278279798306684 12-13 22.88877735716669 28.45945486811066 29.934045648445984 18.71772212627666 14-15 22.20523134735621 28.4716878372574 30.717766639820915 18.60531417556548 16-17 22.384915407007465 28.333100020417184 30.97093400601228 18.31105056656307 18-19 21.894787557096663 28.300314028181866 31.14291142710035 18.66198698762112 20-21 21.99533571220789 29.05654118396607 30.393665048723626 18.55445805510242 22-23 22.279122061709405 28.956225451377932 30.15820195424445 18.60645053266821 24-25 21.574858678483032 29.463961844859437 30.221407789740823 18.739771686916708 26-27 21.44856471209632 29.344531791381563 30.44297269571364 18.76393080080848 28-29 21.208683184070452 29.649417301571567 30.30704161546168 18.8348578988963 30-31 21.195102130270985 29.564701212267014 30.512452026007303 18.7277446314547 32-33 20.611288553379623 29.613407134917498 31.06891947459533 18.706384837107553 34-35 20.592638774563856 30.357566788621583 30.548053861326384 18.50174057548817 36-37 20.754423079227337 29.90634836738706 30.819954370180234 18.51927418320537 38-39 20.897244998724023 29.149477958880247 31.02230186292676 18.930975179468973 40-41 21.097371888175484 29.626091736368178 30.016117653768475 19.260418721687863 42-43 21.4415314648738 29.1744329248878 30.358729270541236 19.025306339697163 44-45 20.78228337165467 29.521722444060522 30.532726656836967 19.163267527447843 46-47 21.118659623854334 29.67774083982152 30.10590298875603 19.097696547568116 48-49 20.758680358860257 29.79310462681345 30.159026773148355 19.28918824117794 50-51 20.909568499121978 29.317294836442663 30.050785959442255 19.7223507049931 52-53 21.421731149546382 29.20976689755792 29.747354175317415 19.621147777578287 54-55 21.507040554932217 29.353484042048617 29.72094179688839 19.418533606130776 56-57 21.46679059833944 29.137931091874357 29.90411199209808 19.491166317688126 58-59 21.378397757917732 29.433487248495492 29.85542536801012 19.332689625576656 60-61 20.777500203540473 29.63882048129613 29.543450400348025 20.040228914815373 62-63 21.049715901529094 29.644592990344908 29.44907565962838 19.856615448497614 64-65 20.662389546104553 29.71571063247602 29.83319318532432 19.788706636095103 66-67 20.868940970704756 29.21161019359344 29.781080969560048 20.138367866141756 68-69 20.72664331076311 29.22723090566149 30.588221592323944 19.457904191251462 70-71 20.706532268859643 30.291675210795532 29.767149799493463 19.23464272085136 72-73 20.648042697917557 30.547019939519416 29.43130064306364 19.373636719499387 74-75 20.805222351741754 30.108725800250124 29.435936461520225 19.650115386487897 76-77 20.92999656756219 29.928950200817024 29.44683408904265 19.69421914257814 78-79 20.65098878708097 29.79675495996131 29.888474137798138 19.663782115159584 80-81 20.642924683953225 30.03876027030699 29.65570520232817 19.662609843411612 82-83 20.333275713357857 30.06932814738761 29.557383171431557 20.040012967822978 84-85 20.39112922147227 30.109388785294932 29.635400667889826 19.864081325342973 86-87 20.461553464862874 29.904796055023493 30.038064492293522 19.595585987820115 88-89 20.624766246317773 30.30231014929503 29.646562768251595 19.426360836135604 90-91 20.931159951609615 29.97054691207357 29.31033717751298 19.787955958803835 92-93 20.54885839613965 30.25483954930943 29.313851037267685 19.882451017283227 94-95 20.15756000687146 30.609529168707716 29.273343300422567 19.959567523998256 96-97 20.408957074280604 30.726249176066688 29.091758470833014 19.773035278819698 98-99 20.622251430348935 30.314489284391776 28.977115526399693 20.0861437588596 100-101 20.64197261859407 30.188398326876765 28.900228344798652 20.269400709730515 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1146.0 1 781.0 2 360.5 3 321.0 4 331.5 5 416.5 6 638.0 7 917.5 8 1222.5 9 1508.5 10 1725.0 11 1797.5 12 1702.0 13 1497.5 14 1262.0 15 1069.5 16 936.5 17 840.0 18 908.0 19 1124.0 20 1437.0 21 1968.5 22 2774.0 23 4139.5 24 6108.0 25 8872.0 26 12977.5 27 18443.5 28 25149.0 29 32233.0 30 39684.5 31 48352.0 32 62265.0 33 84861.0 34 113222.5 35 150110.0 36 196699.5 37 252511.5 38 304104.0 39 319356.0 40 324097.0 41 311808.0 42 278305.5 43 264182.0 44 247452.0 45 232153.0 46 233848.0 47 215872.0 48 171103.0 49 128838.0 50 98103.5 51 78426.0 52 62437.0 53 46761.5 54 36016.0 55 27863.5 56 19107.5 57 12430.5 58 8242.5 59 5703.0 60 4130.0 61 3076.0 62 2127.5 63 1405.5 64 1011.5 65 705.5 66 500.5 67 373.0 68 270.0 69 203.5 70 175.5 71 133.5 72 94.0 73 89.0 74 70.5 75 54.5 76 51.5 77 43.0 78 31.5 79 32.0 80 33.0 81 20.5 82 11.0 83 10.5 84 11.0 85 11.0 86 8.0 87 5.0 88 4.0 89 2.5 90 2.5 91 2.5 92 2.0 93 1.5 94 0.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2381345112847456 2 0.23123590705811234 3 0.2117119726603008 4 0.20131984449838525 5 0.20494603389956426 6 0.1334172368702094 7 0.10825502017178408 8 0.10250618331625633 9 0.09591713184338223 10-11 0.07554192737273291 12-13 0.10517054808968362 14-15 0.10766908103073992 16-17 0.09732117469078996 18-19 0.09088689959479546 20-21 0.0851380627392677 22-23 0.16599766420336376 24-25 0.6412164185011721 26-27 0.36873260700464816 28-29 0.188528682494836 30-31 0.32309568673615097 32-33 0.3612259527259879 34-35 0.34324978209697227 36-37 0.3527132519976103 38-39 0.3604962926635555 40-41 0.3629506037826462 42-43 0.3829720336775707 44-45 0.35915858254909616 46-47 0.3836574719180375 48-49 0.37096580901391085 50-51 0.37761013776424196 52-53 0.3710431972023506 54-55 0.35407307302305235 56-57 0.3627847719502752 58-59 0.35268008563113606 60-61 0.3305802301038284 62-63 0.3174684598910286 64-65 0.31673879982859626 66-67 0.3307792283026736 68-69 0.3288224126806959 70-71 0.31391965867828936 72-73 0.30444513332215994 74-75 0.3119960094227858 76-77 0.31389754776730655 78-79 0.3167940771060532 80-81 0.32760631257664197 82-83 0.3563173304878065 84-85 0.3421331810923409 86-87 0.35290119474096404 88-89 0.35467006761958797 90-91 0.334913968656457 92-93 0.3128804458620978 94-95 0.312858334951115 96-97 0.321769032077183 98-99 0.34353722393974867 100-101 0.3509664900299691 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4522654.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.38320832614167 #Duplication Level Percentage of deduplicated Percentage of total 1 90.29022646840299 44.58821063503656 2 4.3892901977875844 4.335144644824718 3 1.5285716267509502 2.264573132558143 4 0.7914349594654455 1.5633438991949435 5 0.4889190571789206 1.2072195827643704 6 0.3247628154081383 0.9622697861930632 7 0.2397665049413193 0.8288307484203634 8 0.2156820625532422 0.8520857781822945 9 0.14140715469863593 0.6284825081360712 >10 1.2834471116269854 12.649358059519185 >50 0.16628398345534542 5.711806083441953 >100 0.11248587318295862 10.843999994795018 >500 0.018033344329500177 6.341100959803386 >1k 0.009643543640551505 6.944991868144588 >5k 0.0 0.0 >10k+ 4.5296577197713224E-5 0.2785823189851996 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT 12454 0.2753692853797792 Illumina Single End PCR Primer 1 (96% over 32bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 8.181037063635644E-4 0.0 0.0 2 9.728800832431577E-4 0.0 0.0 3 0.0013266546589679423 0.0 0.0 4 0.0020784256323831095 0.0 0.0 5 0.0025206438520390903 0.0 0.0 6 0.00484228950523299 2.2110910982799036E-5 0.0 7 0.00665538420582251 2.2110910982799036E-5 0.0 8 0.008313702529532438 2.2110910982799036E-5 0.0 9 0.009352915345723993 2.2110910982799036E-5 0.0 10-11 0.012901716558463238 2.2110910982799036E-5 0.0 12-13 0.019855598062553534 2.2110910982799036E-5 0.0 14-15 0.028125078770120375 2.2110910982799036E-5 0.0 16-17 0.0391252569840629 2.2110910982799036E-5 0.0 18-19 0.0527345226939757 2.2110910982799036E-5 0.0 20-21 0.06791366308366725 2.2110910982799036E-5 0.0 22-23 0.0851270072837763 2.2110910982799036E-5 2.2110910982799036E-5 24-25 0.10394339253013829 2.2110910982799036E-5 3.3166366474198556E-5 26-27 0.12447337337766717 2.2110910982799036E-5 4.422182196559807E-5 28-29 0.14616417705179305 2.2110910982799036E-5 4.422182196559807E-5 30-31 0.16858464078835125 2.2110910982799036E-5 4.422182196559807E-5 32-33 0.19196692915266123 2.2110910982799036E-5 4.422182196559807E-5 34-35 0.21750503133779414 2.2110910982799036E-5 4.422182196559807E-5 36-37 0.24497783823392194 2.2110910982799036E-5 4.422182196559807E-5 38-39 0.2766848845832558 2.2110910982799036E-5 5.5277277456997596E-5 40-41 0.31040402383202426 2.2110910982799036E-5 6.633273294839711E-5 42-43 0.34735135608428147 2.2110910982799036E-5 6.633273294839711E-5 44-45 0.39035707794582564 2.2110910982799036E-5 8.844364393119616E-5 46-47 0.4356733900050722 2.2110910982799036E-5 1.1055455491399519E-4 48-49 0.4853013297059647 2.2110910982799036E-5 1.3266546589679423E-4 50-51 0.536642865008024 2.2110910982799036E-5 1.3266546589679423E-4 52-53 0.5917211442661765 2.2110910982799036E-5 1.3266546589679423E-4 54-55 0.64854618549197 2.2110910982799036E-5 1.4372092138819373E-4 56-57 0.7103461816888933 2.2110910982799036E-5 1.5477637687959326E-4 58-59 0.7748326535702267 2.2110910982799036E-5 1.5477637687959326E-4 60-61 0.8440287494908962 2.2110910982799036E-5 1.5477637687959326E-4 62-63 0.9163203729491577 2.2110910982799036E-5 1.5477637687959326E-4 64-65 0.9908673093276648 2.2110910982799036E-5 1.658318323709928E-4 66-67 1.0678906677362452 2.2110910982799036E-5 1.768872878623923E-4 68-69 1.1496125947286704 2.2110910982799036E-5 1.8794274335379182E-4 70-71 1.2341094410494369 2.2110910982799036E-5 1.9899819884519135E-4 72-73 1.3207731566465177 2.2110910982799036E-5 2.3216456531938991E-4 74-75 1.4126882136020131 2.2110910982799036E-5 2.4322002081078942E-4 76-77 1.5085832345344126 2.2110910982799036E-5 2.4322002081078942E-4 78-79 1.6074190066275245 2.2110910982799036E-5 2.4322002081078942E-4 80-81 1.7141483739414953 3.3166366474198556E-5 2.4322002081078942E-4 82-83 1.828373340078635 4.422182196559807E-5 2.4322002081078942E-4 84-85 1.9499944059395213 4.422182196559807E-5 2.4322002081078942E-4 86-87 2.077408530477901 4.422182196559807E-5 2.4322002081078942E-4 88-89 2.207630740711096 4.422182196559807E-5 2.4322002081078942E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 830 0.0 62.865696 2 GAGCGGC 485 0.0 59.58263 9 CGGGAGA 850 0.0 55.800343 4 CGAGGCG 190 0.0 49.942677 1 CGCGAGC 40 5.420435E-4 47.44554 1 CGAGCGC 50 2.8475622E-5 47.445538 1 AGAGCGG 630 0.0 46.62164 8 CGACCGC 80 2.2850509E-7 41.514847 1 GAGAGCG 910 0.0 40.606842 7 GATCCGG 205 0.0 39.34508 1 GATCGGG 1645 0.0 38.648647 1 CGGGTCG 130 2.0918378E-10 36.49657 1 CGATCGC 55 0.002594979 34.505848 1 CGGACCG 55 0.002594979 34.505848 1 CGGAAAG 405 0.0 33.961304 5 CGGACGC 70 2.0681703E-4 33.88967 1 CGGAGCG 85 1.6461305E-5 33.49097 1 AAGCGGC 260 0.0 32.796745 9 CGAGGGG 565 0.0 32.75002 1 GGTACGG 145 6.730261E-10 32.72106 1 >>END_MODULE