FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n02_34274-2.3420000003583a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n02_34274-2.3420000003583a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4522654
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT124540.2753692853797792Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA8300.062.8656962
GAGCGGC4850.059.582639
CGGGAGA8500.055.8003434
CGAGGCG1900.049.9426771
CGCGAGC405.420435E-447.445541
CGAGCGC502.8475622E-547.4455381
AGAGCGG6300.046.621648
CGACCGC802.2850509E-741.5148471
GAGAGCG9100.040.6068427
GATCCGG2050.039.345081
GATCGGG16450.038.6486471
CGGGTCG1302.0918378E-1036.496571
CGATCGC550.00259497934.5058481
CGGACCG550.00259497934.5058481
CGGAAAG4050.033.9613045
CGGACGC702.0681703E-433.889671
CGGAGCG851.6461305E-533.490971
AAGCGGC2600.032.7967459
CGAGGGG5650.032.750021
GGTACGG1456.730261E-1032.721061
Produced by FastQC (version 0.11.2)