##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n02_34271-1.342000000358da.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5114626 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.173882899746726 33.0 33.0 33.0 27.0 33.0 2 30.754272746433465 33.0 33.0 33.0 27.0 33.0 3 30.611165312967167 33.0 33.0 33.0 27.0 33.0 4 34.00865283209369 37.0 37.0 37.0 27.0 37.0 5 34.06816255968667 37.0 37.0 37.0 27.0 37.0 6 34.57053262545492 37.0 37.0 37.0 27.0 37.0 7 33.97249495857566 37.0 37.0 37.0 27.0 37.0 8 34.29794749410807 37.0 37.0 37.0 27.0 37.0 9 33.82137188525613 37.0 37.0 37.0 27.0 37.0 10-11 33.8065815565009 37.0 37.0 37.0 24.5 37.0 12-13 33.795968268256566 37.0 37.0 37.0 24.5 37.0 14-15 35.30517461100773 40.0 37.0 40.0 22.0 40.0 16-17 35.20004229048224 40.0 37.0 40.0 18.5 40.0 18-19 35.22581181888959 40.0 37.0 40.0 18.5 40.0 20-21 35.22918254433462 40.0 37.0 40.0 22.0 40.0 22-23 35.9029299503033 40.0 37.0 40.0 27.0 40.0 24-25 35.134307963084694 38.5 37.0 40.0 22.0 40.0 26-27 35.06598810939451 37.0 37.0 40.0 21.0 40.0 28-29 34.81778139789694 37.0 37.0 40.0 18.5 40.0 30-31 34.74845638762248 37.0 37.0 40.0 18.5 40.0 32-33 34.614166021132334 37.0 37.0 40.0 15.0 40.0 34-35 34.62921668172805 37.0 37.0 40.0 15.0 40.0 36-37 34.44454081295484 37.0 37.0 40.0 15.0 40.0 38-39 34.35763152574597 37.0 37.0 40.0 15.0 40.0 40-41 34.35852259774224 37.0 35.0 40.0 15.0 40.0 42-43 34.02963980161991 37.0 33.0 40.0 15.0 40.0 44-45 33.8401052198147 37.0 33.0 40.0 15.0 40.0 46-47 33.77633604099303 37.0 33.0 40.0 15.0 40.0 48-49 33.64141816038944 37.0 33.0 40.0 15.0 40.0 50-51 32.66978279545758 37.0 33.0 38.5 15.0 38.5 52-53 33.137817408350095 37.0 33.0 38.5 15.0 40.0 54-55 33.76581875194785 37.0 33.0 40.0 15.0 40.0 56-57 33.988886968470425 37.0 33.0 40.0 18.5 40.0 58-59 33.52753133855731 37.0 33.0 40.0 15.0 40.0 60-61 33.26740000148593 37.0 33.0 40.0 15.0 40.0 62-63 32.95365467582576 37.0 33.0 40.0 10.5 40.0 64-65 32.72794755667374 37.0 33.0 38.5 15.0 40.0 66-67 32.604211138800764 37.0 33.0 37.0 15.0 40.0 68-69 32.6305433671983 37.0 33.0 37.0 15.0 40.0 70-71 32.22157299478007 37.0 33.0 37.0 15.0 40.0 72-73 32.28950396373068 37.0 33.0 37.0 15.0 38.5 74-75 32.34042694421841 37.0 33.0 37.0 22.0 37.0 76-77 32.07860476992844 37.0 33.0 37.0 22.0 37.0 78-79 31.697505741377768 37.0 33.0 37.0 15.0 37.0 80-81 31.343074742903976 35.0 33.0 37.0 15.0 37.0 82-83 30.99318767393745 33.0 33.0 37.0 15.0 37.0 84-85 30.75428868112742 33.0 27.0 37.0 15.0 37.0 86-87 30.56371961508036 33.0 27.0 37.0 15.0 37.0 88-89 30.39983285190354 33.0 27.0 37.0 15.0 37.0 90-91 30.2431178741124 33.0 27.0 37.0 15.0 37.0 92-93 30.093622681306513 33.0 27.0 37.0 15.0 37.0 94-95 29.95792703122379 33.0 27.0 37.0 15.0 37.0 96-97 29.750834371858275 33.0 27.0 37.0 10.5 37.0 98-99 29.621343183255238 33.0 27.0 37.0 6.0 37.0 100-101 28.401152107700543 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.08695674674728338 2306 2 -0.2119495375186098 2306 3 -0.13880049418338558 2306 4 -0.022596927362464214 2306 5 -0.12284956736684194 2306 6 -0.13469203178392064 2306 7 0.3749701016018818 2306 8 -0.23340685606549272 2306 9 -0.0768638458488553 2306 10-11 -0.011724356767174982 2306 12-13 -0.00497011573741446 2306 14-15 0.369598057508675 2306 16-17 0.33228412908978555 2306 18-19 0.32441925071662325 2306 20-21 0.25092996042885574 2306 22-23 0.20419805064424423 2306 24-25 0.4401683972465662 2306 26-27 0.12206326194940686 2306 28-29 0.18391740389824207 2306 30-31 0.32495606305306524 2306 32-33 0.28317629708116954 2306 34-35 0.12982431820044837 2306 36-37 0.2520366669065339 2306 38-39 0.32858104864278204 2306 40-41 0.17231461809712556 2306 42-43 0.25628703470898273 2306 44-45 0.428574000119859 2306 46-47 0.29556825434041656 2306 48-49 0.2800992280350201 2306 50-51 0.20219751514611062 2306 52-53 0.2621151510394384 2306 54-55 0.5078366997082 2306 56-57 0.44537776979645827 2306 58-59 0.569357941212246 2306 60-61 0.4609666058507287 2306 62-63 0.5696285866773749 2306 64-65 0.5593879942926989 2306 66-67 0.482029136554452 2306 68-69 0.3669640101927385 2306 70-71 0.47034019144013683 2306 72-73 0.30280970921072736 2306 74-75 0.26052952245212424 2306 76-77 0.32749323868436164 2306 78-79 0.2472445821343463 2306 80-81 0.41303159340104756 2306 82-83 0.4475030950927419 2306 84-85 0.39436637460991975 2306 86-87 0.36557235346658956 2306 88-89 0.5009167493631672 2306 90-91 0.46153369602939165 2306 92-93 0.4186979193065241 2306 94-95 0.4549830862618407 2306 96-97 0.6751073736209356 2306 98-99 0.6933054397118426 2306 100-101 0.4465225782780067 2307 1 -0.23133345255673277 2307 2 -0.3118126460206163 2307 3 -0.3145168552964037 2307 4 -0.20025596771187537 2307 5 -0.32726905872716117 2307 6 -0.31652720907677434 2307 7 -0.009493140117925236 2307 8 -0.3822137403459749 2307 9 -0.21619668069774178 2307 10-11 -0.19571784801989622 2307 12-13 -0.18577253963349705 2307 14-15 0.08276658174114715 2307 16-17 0.06345991536241513 2307 18-19 0.061812597619116616 2307 20-21 -0.025997754997128197 2307 22-23 -0.013743010682333079 2307 24-25 0.17607668105615204 2307 26-27 -0.11478062645192466 2307 28-29 0.10186993160402125 2307 30-31 0.15329835488169152 2307 32-33 0.1338471915886288 2307 34-35 -0.05180798952896737 2307 36-37 0.10144306841163342 2307 38-39 -0.020705048650739855 2307 40-41 -0.025870020294455287 2307 42-43 0.15119703847545196 2307 44-45 0.17759373602206097 2307 46-47 0.04279285250691345 2307 48-49 0.17376459159159907 2307 50-51 0.10003723446102697 2307 52-53 0.05552168613342445 2307 54-55 0.0834261142734718 2307 56-57 0.13277247114180568 2307 58-59 0.24026013002900015 2307 60-61 0.2416276852860193 2307 62-63 0.28922889615828495 2307 64-65 0.2294886869139603 2307 66-67 0.216721872844424 2307 68-69 0.11545065223204887 2307 70-71 0.20458844782689312 2307 72-73 -8.468782983399592E-4 2307 74-75 0.015816655059502693 2307 76-77 0.10421222147922293 2307 78-79 0.05269029587934071 2307 80-81 0.144945346933266 2307 82-83 0.16809600708920414 2307 84-85 0.2716512176522592 2307 86-87 0.21676350246086784 2307 88-89 0.322642791959467 2307 90-91 0.38819718377539303 2307 92-93 0.34300270976679315 2307 94-95 0.24249074443014962 2307 96-97 0.28182555859330094 2307 98-99 0.2803385975860735 2307 100-101 0.31128958627664005 2201 1 0.09954954414704176 2201 2 -0.04443498120284772 2201 3 0.021270437649295815 2201 4 0.15904592839951448 2201 5 0.12814514789528175 2201 6 0.0865208041361285 2201 7 0.5150341089692319 2201 8 0.08436107592712005 2201 9 0.17357367842631533 2201 10-11 0.257048202666347 2201 12-13 0.3335577851126814 2201 14-15 0.6081903523713876 2201 16-17 0.555073388084125 2201 18-19 0.6336913206923853 2201 20-21 0.4826475266023138 2201 22-23 0.4409582796085445 2201 24-25 0.583363317430674 2201 26-27 0.46848312005059256 2201 28-29 0.5807550322161461 2201 30-31 0.6510817895507444 2201 32-33 0.4911407327460324 2201 34-35 0.578279020070589 2201 36-37 0.5470089075334599 2201 38-39 0.5515226810239753 2201 40-41 0.5411194096865444 2201 42-43 0.6640657400448688 2201 44-45 0.6571300733504728 2201 46-47 0.5675282119500409 2201 48-49 0.6420857411449319 2201 50-51 0.609844093247581 2201 52-53 0.5751362038745214 2201 54-55 0.7443725689658578 2201 56-57 0.7856492650767493 2201 58-59 0.87202252468105 2201 60-61 0.7700349489175551 2201 62-63 0.7568837391649978 2201 64-65 0.7990835638651248 2201 66-67 0.7294790176610277 2201 68-69 0.6424990305882758 2201 70-71 0.8288592697481079 2201 72-73 0.5922926414581795 2201 74-75 0.5449228320829249 2201 76-77 0.581362591246851 2201 78-79 0.6174715956335355 2201 80-81 0.6442072586505994 2201 82-83 0.7227581491615638 2201 84-85 0.8204183221597319 2201 86-87 0.710655086767467 2201 88-89 0.7104038292598993 2201 90-91 0.7930541434131193 2201 92-93 0.7973167520947477 2201 94-95 0.7436001439967654 2201 96-97 0.773060051754566 2201 98-99 0.804461561800359 2201 100-101 0.665285735201504 1101 1 0.04034090875599361 1101 2 -0.15776060370440703 1101 3 -0.05344085742331828 1101 4 0.05960119159760069 1101 5 0.07601233460339785 1101 6 0.06403951771390126 1101 7 0.31909659725371853 1101 8 3.4401953021045983E-4 1101 9 0.1988944578220071 1101 10-11 0.17460015705673015 1101 12-13 0.212361421329895 1101 14-15 0.4377687050211492 1101 16-17 0.37931640980450965 1101 18-19 0.4746720773999087 1101 20-21 0.4213804323686432 1101 22-23 0.3324153629979989 1101 24-25 0.42887268359197606 1101 26-27 0.3112522713546042 1101 28-29 0.45317293337956954 1101 30-31 0.46588089368723473 1101 32-33 0.47060682475671456 1101 34-35 0.4349950110675138 1101 36-37 0.4406480267213553 1101 38-39 0.41712857606132303 1101 40-41 0.33006385420812734 1101 42-43 0.48811020553288387 1101 44-45 0.5686253684008733 1101 46-47 0.4745136423657996 1101 48-49 0.4971626018459361 1101 50-51 0.4807922617399001 1101 52-53 0.48127256340848135 1101 54-55 0.5847243328533338 1101 56-57 0.5563119569160904 1101 58-59 0.6173477750632372 1101 60-61 0.6307047082479684 1101 62-63 0.6642385119430756 1101 64-65 0.6616194740788259 1101 66-67 0.5715629416875814 1101 68-69 0.4332290926961164 1101 70-71 0.5988686699277821 1101 72-73 0.5530465441989421 1101 74-75 0.48264976588872344 1101 76-77 0.5040818674468994 1101 78-79 0.572669395130621 1101 80-81 0.6068682731757526 1101 82-83 0.5942027082348247 1101 84-85 0.6030061902350816 1101 86-87 0.5987804521178788 1101 88-89 0.6424969035352 1101 90-91 0.7212191366996983 1101 92-93 0.7694293209819563 1101 94-95 0.624671903329947 1101 96-97 0.810728807195769 1101 98-99 0.6878368511301147 1101 100-101 0.6927212078972467 2304 1 -0.0057576795762166455 2304 2 -0.059274652156098284 2304 3 -0.057993240844574245 2304 4 -0.030264164875930533 2304 5 -0.10003454901360698 2304 6 0.05717913125089069 2304 7 0.14943800230342674 2304 8 0.07672666430443087 2304 9 0.06562786028755596 2304 10-11 0.14898197385423373 2304 12-13 0.13992473235688152 2304 14-15 0.28243408537798587 2304 16-17 0.26873051951368865 2304 18-19 0.27317712126012594 2304 20-21 0.2204834961747295 2304 22-23 0.24995173693958606 2304 24-25 0.3112067873623019 2304 26-27 0.3366442192065193 2304 28-29 0.3201748852280204 2304 30-31 0.2961959455024967 2304 32-33 0.20731030858263466 2304 34-35 0.22377736538278015 2304 36-37 0.28040522742565344 2304 38-39 0.3310286171403902 2304 40-41 0.23388270802877287 2304 42-43 0.32594406118033703 2304 44-45 0.3646743514554629 2304 46-47 0.3391163747992607 2304 48-49 0.3178223357741956 2304 50-51 0.2459771415330394 2304 52-53 0.34811504510911107 2304 54-55 0.5516695480625131 2304 56-57 0.45378026012229356 2304 58-59 0.5022044016143639 2304 60-61 0.4128673009784123 2304 62-63 0.5604559186293301 2304 64-65 0.5427938150040106 2304 66-67 0.5196206338797111 2304 68-69 0.35397518584289145 2304 70-71 0.3152898665068591 2304 72-73 0.346820327924128 2304 74-75 0.41392951699510405 2304 76-77 0.5216847254165842 2304 78-79 0.5674117594601178 2304 80-81 0.5074830588509052 2304 82-83 0.4888446546119454 2304 84-85 0.38347680056221023 2304 86-87 0.39942242352505986 2304 88-89 0.4942570377183735 2304 90-91 0.5427054139144438 2304 92-93 0.5562465950971109 2304 94-95 0.5114510479535781 2304 96-97 0.6114885687397944 2304 98-99 0.5932967726845817 2304 100-101 0.5511201794374649 2202 1 0.16586106346182916 2202 2 0.05137737711265089 2202 3 0.060670469635404345 2202 4 0.07204244292907447 2202 5 0.07149927219938945 2202 6 0.2634877594393501 2202 7 0.28254079178126545 2202 8 0.2189934006825922 2202 9 0.22230172288193017 2202 10-11 0.23536999815325998 2202 12-13 0.25774499523856775 2202 14-15 0.32390365529253273 2202 16-17 0.3772103140159757 2202 18-19 0.4888069403217017 2202 20-21 0.358529365854352 2202 22-23 0.3866624944130308 2202 24-25 0.3704018353060903 2202 26-27 0.4215930075661021 2202 28-29 0.2979375383531604 2202 30-31 0.49947651844308183 2202 32-33 0.27038517515961047 2202 34-35 0.3840738367977963 2202 36-37 0.4003107117023177 2202 38-39 0.3429542117116071 2202 40-41 0.33120485829172 2202 42-43 0.387562329462547 2202 44-45 0.5147791958902701 2202 46-47 0.32544731248034253 2202 48-49 0.4385455643074394 2202 50-51 0.5023542666072132 2202 52-53 0.5169906620019091 2202 54-55 0.5727956537975913 2202 56-57 0.5242916578407844 2202 58-59 0.6059165034229821 2202 60-61 0.5381153508255494 2202 62-63 0.57728744955363 2202 64-65 0.5729341582736467 2202 66-67 0.558307640659045 2202 68-69 0.5308452979870779 2202 70-71 0.5065801833323036 2202 72-73 0.5233549610603276 2202 74-75 0.5317456975813357 2202 76-77 0.5841213931385951 2202 78-79 0.5019059111435062 2202 80-81 0.6120225058702182 2202 82-83 0.6394273544083049 2202 84-85 0.6005246125072894 2202 86-87 0.5779324088701223 2202 88-89 0.4383651463767819 2202 90-91 0.4259392904349859 2202 92-93 0.5202009090703825 2202 94-95 0.662422699788408 2202 96-97 0.6005571895055333 2202 98-99 0.6179025817002852 2202 100-101 0.6811055315566144 1102 1 0.028916734879967265 1102 2 0.03356902264819084 1102 3 -0.01716270149031729 1102 4 -0.05038071811107869 1102 5 -0.08543411012725954 1102 6 0.1478693180889863 1102 7 0.02079977444160619 1102 8 0.06973619016800825 1102 9 -0.0634901592339645 1102 10-11 0.10267092968840075 1102 12-13 0.08140338931699631 1102 14-15 0.05737654786254609 1102 16-17 0.12197263572553396 1102 18-19 0.2085422550155016 1102 20-21 0.11679395405666781 1102 22-23 0.16211019294080842 1102 24-25 0.12013893759398542 1102 26-27 0.21917445896009724 1102 28-29 0.15653981080456703 1102 30-31 0.2618500902988572 1102 32-33 0.2974217082863788 1102 34-35 0.21757765364223047 1102 36-37 0.1282769592774784 1102 38-39 0.2679658807286245 1102 40-41 0.16314080614693438 1102 42-43 0.19203415609680974 1102 44-45 0.2955511937804687 1102 46-47 0.22355624206458913 1102 48-49 0.2100835904063203 1102 50-51 0.26078506243968747 1102 52-53 0.26064401329945497 1102 54-55 0.4284586029306041 1102 56-57 0.36108500644590436 1102 58-59 0.3797517810407598 1102 60-61 0.38070624226121197 1102 62-63 0.5015745636484752 1102 64-65 0.38578050462994184 1102 66-67 0.3390298452260154 1102 68-69 0.3405965961996955 1102 70-71 0.4138160497526755 1102 72-73 0.539107720244921 1102 74-75 0.5399164922662294 1102 76-77 0.49550638132061664 1102 78-79 0.41606235983402584 1102 80-81 0.5651162298701031 1102 82-83 0.6065862439371941 1102 84-85 0.5869615181598355 1102 86-87 0.475263817781169 1102 88-89 0.4384978366176213 1102 90-91 0.3645908817247694 1102 92-93 0.591023152699595 1102 94-95 0.45154963110727664 1102 96-97 0.41137000964488024 1102 98-99 0.3951853228103168 1102 100-101 0.5191672995533807 2305 1 0.05114932427123264 2305 2 0.006199571660872039 2305 3 -0.05737418176243381 2305 4 -0.0820566179597364 2305 5 -0.07245365793578884 2305 6 0.18541098213684393 2305 7 -0.061838077314696704 2305 8 0.29532250470675336 2305 9 -0.007663203330764645 2305 10-11 0.05745926485241171 2305 12-13 0.025143518890814676 2305 14-15 -0.044440296434380855 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-0.28767548263890674 1213 82-83 -0.2547282356904077 1213 84-85 -0.3167968067523006 1213 86-87 -0.42833878686871074 1213 88-89 -0.28484976963602193 1213 90-91 -0.28040937119850895 1213 92-93 -0.2555213548990203 1213 94-95 -0.22669533140834375 1213 96-97 -0.2536621547230631 1213 98-99 -0.2679666444154236 1213 100-101 -0.140320585421712 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1768.0 3 533.0 4 688.0 5 816.0 6 2053.0 7 8937.0 8 50455.0 9 92002.0 10 89813.0 11 71014.0 12 49964.0 13 36088.0 14 27353.0 15 22754.0 16 20444.0 17 19764.0 18 19474.0 19 20292.0 20 21104.0 21 22147.0 22 24039.0 23 26060.0 24 29853.0 25 35041.0 26 41320.0 27 49125.0 28 57252.0 29 68367.0 30 84230.0 31 108279.0 32 148388.0 33 211704.0 34 316822.0 35 522876.0 36 950034.0 37 1444910.0 38 417727.0 39 1136.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.80454957513078 23.63124149761845 21.524336162659402 15.039872764591369 2 31.48525099121632 26.723474063777232 29.06149832481797 12.729776620188481 3 35.17927338517795 20.343353861555475 28.737891467188316 15.739481286078261 4 28.90445438395136 18.506033735164443 32.134696185866034 20.454815695018162 5 34.60611038770612 17.09734349622124 30.64900561578634 17.647540500286304 6 31.54698003656765 17.40909962923376 34.65652247109629 16.387397863102297 7 35.28581501946023 19.601690996116954 27.16465371118301 17.947840273239795 8 28.55180835595287 30.010450322805383 23.982903741567824 17.454837579673928 9 32.322003852267514 25.418840652595954 23.973526081079996 18.28562941405653 10-11 27.11596400127485 28.877063833192366 27.64897193036208 16.358000235170696 12-13 28.59416515183821 24.112265839257365 27.330907324593923 19.9626616843105 14-15 25.450619556939163 24.43304685958037 29.083209947747008 21.033123635733457 16-17 27.44135390740104 24.78180348390382 29.665920249102427 18.110922359592713 18-19 26.945954640375952 24.885293813771316 29.889571325253677 18.279180220599056 20-21 25.764421855419613 27.351246389589456 28.42993526477518 18.454396490215753 22-23 28.716247077973733 25.05388277626152 27.528529991310414 18.70134015445433 24-25 24.557891864572902 28.836618835515175 27.703557816910152 18.901931483001775 26-27 23.237441712418534 30.18337993462118 27.73164957178843 18.847528781171857 28-29 24.521792762876018 29.140833540703447 27.246680144010345 19.09069355241019 30-31 26.908114152670315 26.30970063929355 27.86247520624365 18.919710001792485 32-33 23.259850877012283 26.14541306047417 31.667475944819984 18.927260117693567 34-35 20.974816391589144 28.76843979240804 28.870252671693137 21.38649114430968 36-37 23.83857907812159 29.789683050350046 27.66714166218089 18.70459620934747 38-39 25.241193623599145 26.825052923648396 27.399387683614236 20.534365769138223 40-41 23.545126007447962 26.149656289817376 27.048814722961556 23.256402979773107 42-43 26.06980966041182 26.054641283083818 28.30680191835822 19.568747138146144 44-45 23.69678548495916 27.946952683843733 28.922464251613082 19.43379757958402 46-47 24.54907950751 28.579894423258867 27.529346686149523 19.341679383081605 48-49 21.669115312789035 28.330276449792745 29.213525473571693 20.787082763846527 50-51 20.63100702410683 26.907896834565335 30.34100201564739 22.12009412568045 52-53 25.910672413390547 26.253943482264315 27.318867636143455 20.516516468201687 54-55 26.747466682621994 26.18819966003376 27.594930128404442 19.469403528939797 56-57 27.364732305412904 26.028790194995167 27.045661969664632 19.5608155299273 58-59 25.93514991363124 26.251905669284586 26.918481640005034 20.89446277707914 60-61 22.76277549637104 26.362022050300602 26.597317044756064 24.277885408572292 62-63 25.27372515059222 26.292557488017632 27.091019336321548 21.3426980250686 64-65 23.122009256224214 27.454222616692874 28.790747972375858 20.633020154707058 66-67 21.61628872463823 27.672899664391124 27.624592698038214 23.08621891293243 68-69 21.25127347601364 26.50957103826503 31.902186059050063 20.336969426671267 70-71 20.975674515791017 29.89785232105282 29.080845415414387 20.045627747741776 72-73 20.543724275699986 33.14497686565453 26.372546001533724 19.938752857111762 74-75 20.751003256109005 33.30174943549992 25.999084249982108 19.94816305840897 76-77 20.638492047773365 33.03263524942652 26.060646586835222 20.26822611596489 78-79 20.598081632352905 32.745011042992914 26.45418689989729 20.202720424756894 80-81 20.430114696991033 32.59202216353333 26.584168590965838 20.393694548509796 82-83 20.12250685590716 32.50713251719641 26.692053974660556 20.67830665223586 84-85 20.262409427752083 32.13999615546062 26.698727379004893 20.898867037782406 86-87 20.156774498626945 31.910036073024113 27.24133713343978 20.69185229490916 88-89 20.238526471428035 31.826566935533467 27.323786114600562 20.611120478437932 90-91 20.58738411595872 31.32012459817598 27.00704593800159 21.085445347863708 92-93 20.419590347700296 31.60953236856353 26.79225577413727 21.1786215095989 94-95 20.383373581627563 31.481127422863974 26.765390192750598 21.370108802757866 96-97 20.674185045386988 31.627836331121134 26.583455396184736 21.114523227307146 98-99 20.909034113249607 31.24433295419806 26.612932569988907 21.23370036256343 100-101 20.965067881469434 31.35999811678761 26.17709356574005 21.497840436002903 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2508.0 1 1686.5 2 780.0 3 805.5 4 867.0 5 871.0 6 1054.0 7 1323.5 8 1622.5 9 1920.5 10 2173.0 11 2269.0 12 2278.5 13 2196.0 14 2008.5 15 1891.0 16 1779.0 17 1699.5 18 1721.0 19 1756.5 20 1888.5 21 2218.0 22 2738.0 23 3518.0 24 4596.0 25 6290.0 26 9281.0 27 13870.0 28 18522.0 29 23158.0 30 28745.0 31 34165.5 32 44643.0 33 64557.0 34 93248.0 35 125191.0 36 163705.0 37 211738.0 38 249948.0 39 260277.5 40 260945.0 41 260910.5 42 261228.5 43 268651.5 44 267527.5 45 258763.0 46 248708.0 47 228131.0 48 201576.0 49 181959.5 50 166918.5 51 155413.0 52 145194.0 53 129762.5 54 111123.5 55 94233.0 56 80187.5 57 68914.5 58 59493.0 59 50733.5 60 41946.0 61 34359.5 62 28186.5 63 23170.5 64 19064.5 65 15331.5 66 12070.5 67 9940.0 68 8647.0 69 6719.0 70 4688.5 71 3568.0 72 2755.0 73 2062.5 74 1654.0 75 1297.5 76 1003.5 77 796.0 78 585.0 79 421.5 80 340.5 81 260.0 82 198.5 83 171.0 84 134.0 85 108.5 86 96.0 87 80.5 88 77.5 89 66.0 90 46.5 91 34.5 92 30.0 93 26.5 94 18.0 95 14.5 96 10.0 97 5.5 98 5.5 99 5.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2276608299414268 2 0.2209350204687498 3 0.20081624736588755 4 0.1905320154396431 5 0.19373850600219839 6 0.12290243705013817 7 0.09727006432141862 8 0.09259719087964595 9 0.08581272609180025 10-11 0.06735585358538435 12-13 0.09505093823086966 14-15 0.09710387426177398 16-17 0.08679031467794517 18-19 0.08168730225826874 20-21 0.0759488572575981 22-23 0.15521173982222747 24-25 0.616604224825041 26-27 0.35235225410421017 28-29 0.17636675682640332 30-31 0.3042255680082962 32-33 0.34198003920521264 34-35 0.32354271847051963 36-37 0.33304487952784817 38-39 0.3406798463856399 40-41 0.3426741271013755 42-43 0.3616588974443097 44-45 0.3402594832935976 46-47 0.36214769173738215 48-49 0.35060237053501075 50-51 0.35679050628530806 52-53 0.3504655081329505 54-55 0.33357277736436647 56-57 0.34164765908592337 58-59 0.3325267575771914 60-61 0.3112642058285396 62-63 0.29834048471970387 64-65 0.29809608757316763 66-67 0.3124861915612207 68-69 0.3105701179323767 70-71 0.2951828735864558 72-73 0.28610107562116954 74-75 0.2929050921807382 76-77 0.29421506088617233 78-79 0.29793967339938443 80-81 0.3089179932217918 82-83 0.3362318183186806 84-85 0.32233050862369994 86-87 0.3333479319895531 88-89 0.3347361077818789 90-91 0.315888199841005 92-93 0.29392178431032884 94-95 0.29388268076688306 96-97 0.30260277095529564 98-99 0.32497977369215264 100-101 0.33147096190415487 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5114626.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.268001624879645 #Duplication Level Percentage of deduplicated Percentage of total 1 92.25841705599063 56.52488846095258 2 3.866580054426452 4.737952661146542 3 1.299581188933904 2.388682271857963 4 0.61330655117889 1.5030426709671025 5 0.3943887462451112 1.2081705172889858 6 0.17413947409149774 0.6401506548956142 7 0.17428399287402974 0.7474622371017594 8 0.14535715954646394 0.7124594149824514 9 0.10560605212319137 0.5823244595772732 >10 0.7656770385432065 9.197191387019389 >50 0.10245858773624728 4.36891691041262 >100 0.08872439856523319 11.440345578015657 >500 0.009790975480351178 4.0047455239500085 >1k 0.001656720478885524 1.558054747589119 >5k 0.0 0.0 >10k+ 3.2003786039431046E-5 0.3856125042429113 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT 19666 0.38450514270251623 Illumina Single End PCR Primer 1 (96% over 32bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0027567998129286483 0.0 0.0 2 0.003128283475663714 0.0 0.0 3 0.0036170777687361696 0.0 1.9551771722898214E-5 4 0.003929906116302541 0.0 1.9551771722898214E-5 5 0.00459466635488108 0.0 1.9551771722898214E-5 6 0.005376737223797009 0.0 1.9551771722898214E-5 7 0.0065107399837251055 0.0 1.9551771722898214E-5 8 0.007625190971930303 0.0 1.9551771722898214E-5 9 0.008622331329798113 0.0 1.9551771722898214E-5 10-11 0.012180753783365587 0.0 1.9551771722898214E-5 12-13 0.015631641492457122 0.0 1.9551771722898214E-5 14-15 0.02013832487458516 0.0 1.9551771722898214E-5 16-17 0.02519245786495435 0.0 1.9551771722898214E-5 18-19 0.03066695394736585 0.0 1.9551771722898214E-5 20-21 0.035623328079120546 0.0 1.9551771722898214E-5 22-23 0.040442839808814954 0.0 1.9551771722898214E-5 24-25 0.04607375006500964 0.0 1.9551771722898214E-5 26-27 0.051685108549481426 0.0 2.932765758434732E-5 28-29 0.05859665985352595 0.0 3.910354344579643E-5 30-31 0.08578339843421591 0.0 3.910354344579643E-5 32-33 0.09446438507918273 0.0 3.910354344579643E-5 34-35 0.10163988530148638 0.0 4.887942930724553E-5 36-37 0.11020356131611578 0.0 5.865531516869464E-5 38-39 0.1194417734551852 0.0 5.865531516869464E-5 40-41 0.12989219544107428 0.0 5.865531516869464E-5 42-43 0.14127132658380104 0.0 5.865531516869464E-5 44-45 0.15348140802475096 0.0 5.865531516869464E-5 46-47 0.1664637844487554 0.0 5.865531516869464E-5 48-49 0.18091254375197718 0.0 5.865531516869464E-5 50-51 0.19599673563619313 0.0 5.865531516869464E-5 52-53 0.21343691601301834 0.0 5.865531516869464E-5 54-55 0.23075000987364472 0.0 5.865531516869464E-5 56-57 0.2490797958638618 0.0 5.865531516869464E-5 58-59 0.2694038625698145 0.0 5.865531516869464E-5 60-61 0.2915169163884124 0.0 7.820708689159286E-5 62-63 0.3156340268086073 0.0 8.798297275304196E-5 64-65 0.3421266774931344 0.0 9.775885861449106E-5 66-67 0.3710828514147466 0.0 9.775885861449106E-5 68-69 0.4020039783945102 0.0 9.775885861449106E-5 70-71 0.4347727478020876 0.0 1.1731063033738929E-4 72-73 0.47125635383701564 0.0 1.1731063033738929E-4 74-75 0.5089912732622093 0.0 1.1731063033738929E-4 76-77 0.5517118944767418 0.0 1.1731063033738929E-4 78-79 0.5980104899165648 0.0 1.1731063033738929E-4 80-81 0.6508296012259743 0.0 1.1731063033738929E-4 82-83 0.7101692284049703 0.0 1.1731063033738929E-4 84-85 0.7743772467429682 0.0 1.1731063033738929E-4 86-87 0.843922898761317 0.0 1.1731063033738929E-4 88-89 0.9222570721691088 0.0 1.1731063033738929E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 5305 0.0 80.21251 9 ATCGGGA 9265 0.0 75.588104 2 AGAGCGG 5830 0.0 74.454475 8 TCGGGAG 8365 0.0 74.017006 3 CGGGAGA 7915 0.0 73.84647 4 ATTCGGA 1010 0.0 71.87578 2 GATCGGG 10035 0.0 71.21635 1 ATCCGGA 1410 0.0 69.99335 2 AAGCGGC 1490 0.0 66.14976 9 GAGCGTC 15905 0.0 63.697906 9 GAGAGCG 8035 0.0 63.516296 7 AGAGCGT 16060 0.0 63.40956 8 GATCCGG 1625 0.0 62.785187 1 CGGAAAG 2640 0.0 62.53512 5 TCGGAGA 1640 0.0 59.882023 4 AAGAGCG 16295 0.0 58.509853 7 TCGGGAA 2095 0.0 57.975292 3 CGGAGAG 3200 0.0 57.818035 5 TCGGAAG 17280 0.0 57.411285 3 CGGAAGA 17130 0.0 56.942394 4 >>END_MODULE