FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n02_34271-1.342000000358da.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n02_34271-1.342000000358da.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5114626
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT196660.38450514270251623Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC53050.080.212519
ATCGGGA92650.075.5881042
AGAGCGG58300.074.4544758
TCGGGAG83650.074.0170063
CGGGAGA79150.073.846474
ATTCGGA10100.071.875782
GATCGGG100350.071.216351
ATCCGGA14100.069.993352
AAGCGGC14900.066.149769
GAGCGTC159050.063.6979069
GAGAGCG80350.063.5162967
AGAGCGT160600.063.409568
GATCCGG16250.062.7851871
CGGAAAG26400.062.535125
TCGGAGA16400.059.8820234
AAGAGCG162950.058.5098537
TCGGGAA20950.057.9752923
CGGAGAG32000.057.8180355
TCGGAAG172800.057.4112853
CGGAAGA171300.056.9423944
Produced by FastQC (version 0.11.2)