##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n02_34211-2.34200000035860.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3402601 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.986316056452107 33.0 33.0 33.0 33.0 33.0 2 31.89064189424502 33.0 33.0 33.0 33.0 33.0 3 31.844747297729 33.0 33.0 33.0 33.0 33.0 4 35.52567197858344 37.0 37.0 37.0 33.0 37.0 5 35.54033105850495 37.0 37.0 37.0 33.0 37.0 6 35.62697036766873 37.0 37.0 37.0 33.0 37.0 7 35.43645346603966 37.0 37.0 37.0 33.0 37.0 8 35.525923256943734 37.0 37.0 37.0 33.0 37.0 9 35.396591901313144 37.0 37.0 37.0 33.0 37.0 10-11 35.31444929922726 37.0 37.0 37.0 33.0 37.0 12-13 35.30791297598513 37.0 37.0 37.0 33.0 37.0 14-15 37.01186665730128 40.0 37.0 40.0 33.0 40.0 16-17 36.95372260808716 40.0 37.0 40.0 33.0 40.0 18-19 36.92685860022965 40.0 37.0 40.0 33.0 40.0 20-21 36.92183847004101 40.0 37.0 40.0 33.0 40.0 22-23 37.06611412857399 40.0 37.0 40.0 33.0 40.0 24-25 36.64437940269811 40.0 37.0 40.0 33.0 40.0 26-27 36.60885128758852 40.0 37.0 40.0 33.0 40.0 28-29 36.53698406013517 40.0 37.0 40.0 33.0 40.0 30-31 36.477967883980526 40.0 37.0 40.0 33.0 40.0 32-33 36.360119214683124 40.0 37.0 40.0 33.0 40.0 34-35 36.390916684030834 40.0 37.0 40.0 33.0 40.0 36-37 36.20841423957731 40.0 37.0 40.0 33.0 40.0 38-39 36.11346849072224 40.0 37.0 40.0 33.0 40.0 40-41 36.163478174490635 38.5 37.0 40.0 27.0 40.0 42-43 35.965670820645734 37.0 37.0 40.0 27.0 40.0 44-45 35.76303069328435 37.0 37.0 40.0 27.0 40.0 46-47 35.6548963572279 37.0 37.0 40.0 27.0 40.0 48-49 35.52106388612711 37.0 37.0 40.0 27.0 40.0 50-51 34.47336684495185 37.0 35.0 38.5 24.5 38.5 52-53 34.917667102313786 37.0 35.0 38.5 27.0 40.0 54-55 35.59092617676889 37.0 37.0 40.0 27.0 40.0 56-57 35.6960072603282 37.0 37.0 40.0 27.0 40.0 58-59 35.47875992512786 37.0 37.0 40.0 27.0 40.0 60-61 35.289435787504914 37.0 37.0 40.0 27.0 40.0 62-63 34.98436651843693 37.0 33.0 40.0 27.0 40.0 64-65 34.79047778449486 37.0 33.0 40.0 27.0 40.0 66-67 34.62345834848106 37.0 33.0 40.0 27.0 40.0 68-69 34.506272260544215 37.0 33.0 40.0 27.0 40.0 70-71 34.1395078353295 37.0 33.0 37.0 27.0 40.0 72-73 33.978204467699854 37.0 33.0 37.0 27.0 40.0 74-75 33.81241159336637 37.0 33.0 37.0 27.0 40.0 76-77 33.48333186876745 37.0 33.0 37.0 24.5 40.0 78-79 33.06103683623205 37.0 33.0 37.0 22.0 37.0 80-81 32.68964124797471 37.0 33.0 37.0 22.0 37.0 82-83 32.33415055129885 37.0 33.0 37.0 22.0 37.0 84-85 32.09335549481118 37.0 33.0 37.0 22.0 37.0 86-87 31.886464354768602 37.0 33.0 37.0 18.5 37.0 88-89 31.675011998174337 33.0 33.0 37.0 15.0 37.0 90-91 31.467494719480776 33.0 33.0 37.0 15.0 37.0 92-93 31.28593875685101 33.0 33.0 37.0 15.0 37.0 94-95 31.122886726948003 33.0 33.0 37.0 15.0 37.0 96-97 30.894930378260632 33.0 30.0 37.0 15.0 37.0 98-99 30.747043217820718 33.0 27.0 37.0 15.0 37.0 100-101 29.38696426645381 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.12192227533149946 2306 2 0.09019213631738054 2306 3 0.06257890027557167 2306 4 0.14866638078567718 2306 5 0.0643028113088775 2306 6 0.12882949126581167 2306 7 0.22779421289625645 2306 8 0.136326120226407 2306 9 0.18081985340684525 2306 10-11 0.18530226119403892 2306 12-13 0.1439578555686296 2306 14-15 0.4689043915166451 2306 16-17 0.4823715314071606 2306 18-19 0.4309885802104745 2306 20-21 0.45868616701171305 2306 22-23 0.508535033390892 2306 24-25 0.7455164010098727 2306 26-27 0.5003313739459045 2306 28-29 0.518821466523363 2306 30-31 0.5889367930664093 2306 32-33 0.491673882001713 2306 34-35 0.441683067360799 2306 36-37 0.619143728993393 2306 38-39 0.46670305109116583 2306 40-41 0.4636348288349481 2306 42-43 0.5521763811860083 2306 44-45 0.6087539202779908 2306 46-47 0.5159515323299004 2306 48-49 0.5732383119219193 2306 50-51 0.41700708918052243 2306 52-53 0.5128916349002068 2306 54-55 0.6963899558642268 2306 56-57 0.5862233238117582 2306 58-59 0.5872006116661765 2306 60-61 0.43225072337268955 2306 62-63 0.539008438383199 2306 64-65 0.6368909221373258 2306 66-67 0.5983012792769529 2306 68-69 0.4854505698398057 2306 70-71 0.5758106994314787 2306 72-73 0.6126015287834861 2306 74-75 0.5872087443363725 2306 76-77 0.6227554328520313 2306 78-79 0.6896759319552999 2306 80-81 0.7138621403591046 2306 82-83 0.6764898364563194 2306 84-85 0.6951621616854382 2306 86-87 0.6377290369058031 2306 88-89 0.6714565699407622 2306 90-91 0.6648244307384097 2306 92-93 0.7302839236115197 2306 94-95 0.5119243679143821 2306 96-97 0.6808389486385842 2306 98-99 0.5344587592843446 2306 100-101 0.5013613075134487 2307 1 0.2579854059766191 2307 2 0.2850441017474985 2307 3 0.2513847282556654 2307 4 0.3933629472022915 2307 5 0.3686597533175586 2307 6 0.3754167547517824 2307 7 0.5506890869604604 2307 8 0.46969711945023107 2307 9 0.383264870459918 2307 10-11 0.470615612010441 2307 12-13 0.4785331835394686 2307 14-15 0.7522246071048784 2307 16-17 0.6857276057940354 2307 18-19 0.7146930749704552 2307 20-21 0.618651462139205 2307 22-23 0.5548194456995716 2307 24-25 0.7503019285990504 2307 26-27 0.6549349404485127 2307 28-29 0.683214027836577 2307 30-31 0.8137968250933056 2307 32-33 0.642531543037002 2307 34-35 0.5596274894887472 2307 36-37 0.5176981643697189 2307 38-39 0.46524986620062947 2307 40-41 0.5103141886889873 2307 42-43 0.6550995155541699 2307 44-45 0.5978238449353199 2307 46-47 0.3947000232449014 2307 48-49 0.4602224606782386 2307 50-51 0.40360630559936084 2307 52-53 0.4548540495668263 2307 54-55 0.41561969928984155 2307 56-57 0.42905205395960877 2307 58-59 0.4414185926655847 2307 60-61 0.4851321095645531 2307 62-63 0.4342218221844689 2307 64-65 0.551100869198244 2307 66-67 0.4188217870480031 2307 68-69 0.35908172497428126 2307 70-71 0.4110969983005148 2307 72-73 0.32023485895985004 2307 74-75 0.28365737339593977 2307 76-77 0.4405938244096461 2307 78-79 0.6302282706696403 2307 80-81 0.6542923370817348 2307 82-83 0.6865905001815591 2307 84-85 0.593884351793676 2307 86-87 0.49456087829974393 2307 88-89 0.6002203558199675 2307 90-91 0.7047147859843683 2307 92-93 0.5361303012088037 2307 94-95 0.6524531926623993 2307 96-97 0.5635451884396723 2307 98-99 0.5719554798480679 2307 100-101 0.6705041254663655 2201 1 0.1790939152734765 2201 2 0.1586481601879477 2201 3 0.15868276851460195 2201 4 0.21102644004535165 2201 5 0.17182928928836816 2201 6 0.29662056645838675 2201 7 0.3966667503363652 2201 8 0.32376320943012615 2201 9 0.34015914227836674 2201 10-11 0.47848312801842496 2201 12-13 0.5113739261966543 2201 14-15 0.709962470908188 2201 16-17 0.702096622961129 2201 18-19 0.7608711852347554 2201 20-21 0.6769702029000797 2201 22-23 0.5975398715006079 2201 24-25 0.6518783162089576 2201 26-27 0.5380631007514509 2201 28-29 0.610772990112217 2201 30-31 0.6036049242389936 2201 32-33 0.6593929559878049 2201 34-35 0.5167302962324172 2201 36-37 0.5732155210043999 2201 38-39 0.6212331132453315 2201 40-41 0.47023387350891 2201 42-43 0.5400968893783613 2201 44-45 0.4318301419104955 2201 46-47 0.43270307720964496 2201 48-49 0.4013861830071619 2201 50-51 0.30265493808774124 2201 52-53 0.3936626914879966 2201 54-55 0.5811356234649807 2201 56-57 0.6815712825362965 2201 58-59 0.684364898820192 2201 60-61 0.6519356881797833 2201 62-63 0.7056998962962169 2201 64-65 0.7052905209936355 2201 66-67 0.6191416055641454 2201 68-69 0.4640553803868883 2201 70-71 0.614750354787077 2201 72-73 0.568623439431434 2201 74-75 0.42340919734568416 2201 76-77 0.5738372148757023 2201 78-79 0.7160342532832829 2201 80-81 0.7503026936667112 2201 82-83 0.7628860234044552 2201 84-85 0.7688041291875152 2201 86-87 0.7533837083915245 2201 88-89 0.6619371439533559 2201 90-91 0.8005856970188923 2201 92-93 0.7192321510305213 2201 94-95 0.8321788230663998 2201 96-97 0.6656840356703029 2201 98-99 0.6592376753505356 2201 100-101 0.6203642414078843 1101 1 0.16367350281159787 1101 2 0.04747264123840722 1101 3 0.08688662854629925 1101 4 0.1341249797148265 1101 5 0.08444014998326566 1101 6 0.2068497974524277 1101 7 0.21133231138637854 1101 8 0.10719192958085699 1101 9 0.17380493625149285 1101 10-11 0.25711875786972627 1101 12-13 0.27997037813561576 1101 14-15 0.42730684657343687 1101 16-17 0.3847822511263459 1101 18-19 0.3972072073839854 1101 20-21 0.41501376292806214 1101 22-23 0.40965640395705805 1101 24-25 0.42342958341684067 1101 26-27 0.48964478682837864 1101 28-29 0.417990943297589 1101 30-31 0.3144643221390666 1101 32-33 0.43788142007702646 1101 34-35 0.29766523980013915 1101 36-37 0.42002322584971097 1101 38-39 0.38955122993861835 1101 40-41 0.5045526352413106 1101 42-43 0.5847887621448251 1101 44-45 0.567527782848515 1101 46-47 0.2931638219099071 1101 48-49 0.34084268999062317 1101 50-51 0.3709979570956463 1101 52-53 0.41580387550659026 1101 54-55 0.49795817458606706 1101 56-57 0.5047634652846966 1101 58-59 0.4832601180081184 1101 60-61 0.41163717168329583 1101 62-63 0.48590653362773395 1101 64-65 0.45192486076670235 1101 66-67 0.42220398499760137 1101 68-69 0.4028810287333471 1101 70-71 0.5044188340770788 1101 72-73 0.5055403426556779 1101 74-75 0.3741303701063572 1101 76-77 0.47197576685798026 1101 78-79 0.6137548268320074 1101 80-81 0.640357937030636 1101 82-83 0.5933197646573021 1101 84-85 0.5785191446779692 1101 86-87 0.48363500560324724 1101 88-89 0.5340040101267611 1101 90-91 0.4711483077498819 1101 92-93 0.6164279601784948 1101 94-95 0.5375606628805301 1101 96-97 0.549703407595878 1101 98-99 0.5124396547386816 1101 100-101 0.5400356000280198 2304 1 0.08146177088155326 2304 2 0.0026962908282541775 2304 3 0.08151903838997754 2304 4 0.02806554619060364 2304 5 0.057254729769674384 2304 6 0.24202237135739324 2304 7 0.2456010004437985 2304 8 0.29196905386501726 2304 9 0.18903322908541753 2304 10-11 0.1862431194236649 2304 12-13 0.21925531854731162 2304 14-15 0.3590739878828302 2304 16-17 0.3568430776237932 2304 18-19 0.35920380233832105 2304 20-21 0.31158666186011175 2304 22-23 0.2700688788061498 2304 24-25 0.40996920576632334 2304 26-27 0.4548529589379555 2304 28-29 0.4691668448608155 2304 30-31 0.3175230591766791 2304 32-33 0.40051994352540277 2304 34-35 0.40476746855729573 2304 36-37 0.2797067113782674 2304 38-39 0.3339189748697393 2304 40-41 0.48170787436838225 2304 42-43 0.4188388317934866 2304 44-45 0.3679023378709587 2304 46-47 0.3472475550781482 2304 48-49 0.3874852375838742 2304 50-51 0.22003138845301606 2304 52-53 0.21471232621081526 2304 54-55 0.5450000411701836 2304 56-57 0.5428314888104637 2304 58-59 0.4528994927179184 2304 60-61 0.3928370633409273 2304 62-63 0.3274016582214472 2304 64-65 0.40932038777490476 2304 66-67 0.331860771872698 2304 68-69 0.35722362275903663 2304 70-71 0.3796579611471955 2304 72-73 0.4107001476394174 2304 74-75 0.5170447796404858 2304 76-77 0.5023503218804279 2304 78-79 0.526856126940352 2304 80-81 0.5634816794853705 2304 82-83 0.34224678833785305 2304 84-85 0.3715566001802131 2304 86-87 0.44347287899746135 2304 88-89 0.3911217163641858 2304 90-91 0.4759606433016579 2304 92-93 0.5488819146746984 2304 94-95 0.3766556172219637 2304 96-97 0.61405103180266 2304 98-99 0.5032030814528134 2304 100-101 0.5763322259373211 2202 1 0.07159431009806383 2202 2 0.04332766962757262 2202 3 0.08058623073961257 2202 4 0.13056673456965484 2202 5 0.08030746583767723 2202 6 0.15188591748048452 2202 7 0.2523837088295622 2202 8 0.2548626869494939 2202 9 0.12612547526163098 2202 10-11 0.2549295411444348 2202 12-13 0.17243150622731918 2202 14-15 0.28068871539450413 2202 16-17 0.38206313746217546 2202 18-19 0.40918339018050176 2202 20-21 0.3374255875654555 2202 22-23 0.35742802093628967 2202 24-25 0.14936893453628386 2202 26-27 0.31142175909455005 2202 28-29 0.33919194063019376 2202 30-31 0.33055782974961545 2202 32-33 0.27414428991692574 2202 34-35 0.247694166541244 2202 36-37 0.2621789592321875 2202 38-39 0.33916238286123956 2202 40-41 0.39027818446540863 2202 42-43 0.33818445436430267 2202 44-45 0.2708310630897586 2202 46-47 0.17019291760366428 2202 48-49 0.2499681808492511 2202 50-51 0.21194439910648555 2202 52-53 0.32201058139074945 2202 54-55 0.3316381168349736 2202 56-57 0.3712289247078999 2202 58-59 0.3703639112510402 2202 60-61 0.2925700974823613 2202 62-63 0.41861883798824095 2202 64-65 0.38557664424109106 2202 66-67 0.3312224725130193 2202 68-69 0.37245521686467953 2202 70-71 0.41262890877462866 2202 72-73 0.3809106305407468 2202 74-75 0.3502548011711184 2202 76-77 0.3949809736713803 2202 78-79 0.46342485719228677 2202 80-81 0.3536570702523605 2202 82-83 0.5015062883797938 2202 84-85 0.3782605959300156 2202 86-87 0.2738342161336007 2202 88-89 0.0923894371929741 2202 90-91 0.19718851847991203 2202 92-93 0.28282327865423085 2202 94-95 0.2024021289799265 2202 96-97 0.3390000014081167 2202 98-99 0.3744913542487218 2202 100-101 0.35104107306983323 1102 1 0.01170318122620273 1102 2 -0.014890664542594578 1102 3 0.012718148983442035 1102 4 0.039964005611430764 1102 5 0.028444575711759512 1102 6 0.12962097371855918 1102 7 0.07328960227555115 1102 8 0.22533741640406646 1102 9 0.1303053980726574 1102 10-11 0.13417829333440068 1102 12-13 0.1330712961003897 1102 14-15 0.1663095482618857 1102 16-17 0.1438797127024074 1102 18-19 0.19675528732958725 1102 20-21 0.15668076424774569 1102 22-23 0.2122402483863297 1102 24-25 0.043166172254757385 1102 26-27 0.22867158353428607 1102 28-29 0.16480306170659986 1102 30-31 0.21831121630516748 1102 32-33 0.27055184039659963 1102 34-35 0.3164362969759509 1102 36-37 0.18618482033056694 1102 38-39 0.15883347537268122 1102 40-41 0.33411403668507234 1102 42-43 0.2983280847978804 1102 44-45 0.32711510720760373 1102 46-47 0.2581075794309484 1102 48-49 0.23002229618940362 1102 50-51 0.2685307796330747 1102 52-53 0.2560285684682171 1102 54-55 0.346001285280515 1102 56-57 0.4351197253502477 1102 58-59 0.37919733802471 1102 60-61 0.25751796227681467 1102 62-63 0.3295510573426341 1102 64-65 0.3195754303922982 1102 66-67 0.35796154480932785 1102 68-69 0.3722155492771222 1102 70-71 0.42318435216842687 1102 72-73 0.526384540672403 1102 74-75 0.42556402364493806 1102 76-77 0.3908542130340038 1102 78-79 0.2982851332403129 1102 80-81 0.2835900759065595 1102 82-83 0.3885590600303672 1102 84-85 0.3772617725027274 1102 86-87 0.42173469804557584 1102 88-89 0.32199749062433725 1102 90-91 0.2066672714979596 1102 92-93 0.3210289314569543 1102 94-95 0.26698954172829303 1102 96-97 0.4312091661665747 1102 98-99 0.44276565755530584 1102 100-101 0.39299734849542745 2305 1 0.15449386526811537 2305 2 0.023911099074762632 2305 3 0.07855640384232743 2305 4 -0.036868319175539455 2305 5 0.11120322603805732 2305 6 0.2052690030478317 2305 7 -0.017444641074462197 2305 8 0.10144576511406456 2305 9 0.007890590104160822 2305 10-11 0.10687718676522451 2305 12-13 0.12234702031337008 2305 14-15 0.10687313318197766 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0.23980012328939893 2305 82-83 0.23194524150171958 2305 84-85 0.32031432820306804 2305 86-87 0.04165910279606777 2305 88-89 0.17496337502572956 2305 90-91 0.11291333918086366 2305 92-93 0.21009241247021393 2305 94-95 0.13284031388937123 2305 96-97 0.3343902027182004 2305 98-99 0.21716580220948245 2305 100-101 0.22261323039555947 2203 1 0.03754743679839123 2203 2 0.05716395159679166 2203 3 0.1479340102134472 2203 4 0.09576457914438663 2203 5 0.03717507020704858 2203 6 0.2101852154472823 2203 7 0.016876944879228972 2203 8 0.21225510938321435 2203 9 0.13473045317577004 2203 10-11 0.2002033128329117 2203 12-13 0.12339668818160021 2203 14-15 0.18567593357055046 2203 16-17 0.1358327810165676 2203 18-19 0.1895681259857298 2203 20-21 0.1927974988073089 2203 22-23 0.19282287337956205 2203 24-25 0.15664060717167416 2203 26-27 0.15625111760774502 2203 28-29 0.2274146243650037 2203 30-31 0.18255140963237437 2203 32-33 0.15789204760462638 2203 34-35 0.1756299833412811 2203 36-37 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-1.260876370446823 1213 58-59 -0.7588986525983756 1213 60-61 -0.5451701688031747 1213 62-63 -0.5756399108241297 1213 64-65 -0.4226373767840812 1213 66-67 -0.3269666176810304 1213 68-69 -0.3713814713079344 1213 70-71 -0.38472397462631847 1213 72-73 -0.3849930088302216 1213 74-75 -0.33883184601363325 1213 76-77 -0.4414767520123064 1213 78-79 -0.4655820736516887 1213 80-81 -0.5728045689187908 1213 82-83 -0.4832562700664482 1213 84-85 -0.48740024545454474 1213 86-87 -0.5075047932482235 1213 88-89 -0.391216875647487 1213 90-91 -0.43942477877216746 1213 92-93 -0.38078622040014665 1213 94-95 -0.27984991035277673 1213 96-97 -0.2889070624466967 1213 98-99 -0.3779277915953507 1213 100-101 -0.172712198086586 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1242.0 3 220.0 4 297.0 5 395.0 6 1188.0 7 1955.0 8 6041.0 9 14229.0 10 20594.0 11 19403.0 12 13319.0 13 10457.0 14 9207.0 15 8709.0 16 8651.0 17 9358.0 18 10264.0 19 11353.0 20 12945.0 21 14555.0 22 16580.0 23 18770.0 24 21363.0 25 25555.0 26 30970.0 27 39034.0 28 48292.0 29 56130.0 30 62486.0 31 77454.0 32 101507.0 33 141616.0 34 207942.0 35 328959.0 36 566038.0 37 956845.0 38 525447.0 39 3231.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.66350676276388 28.946408697532387 16.155632239032492 18.23445230067124 2 31.760375483301612 26.953811005874144 27.01242872350641 14.273384787317836 3 35.120531550425106 23.2942355192945 23.83318396902254 17.752048961257852 4 30.248641358361883 23.55141783144451 26.94746978301488 19.25247102717872 5 33.669558426275735 21.355250591844314 26.83926718792747 18.135923793952482 6 31.719274250170525 21.80524649634259 28.309281153610062 18.16619809987682 7 33.78521459800072 23.044502949731445 23.000432758685108 20.169849693582727 8 29.335831514562084 28.55669432846376 22.603509632795014 19.50396452417914 9 30.165124185836632 24.113884916084768 25.016928950181526 20.704061947897074 10-11 26.068594824146963 29.804601526195352 27.702476460996113 16.424327188661575 12-13 26.51516020704902 28.035681977644707 27.26070437265294 18.188453442653334 14-15 24.82482971332883 28.073875569595785 28.256363924673465 18.844930792401918 16-17 25.646357158090588 27.922693945388627 28.31879922635113 18.112149670169657 18-19 25.039507772497 27.96853203162975 28.688827297535997 18.303132898337253 20-21 24.435095985922896 29.151631065805155 27.91163427183131 18.501638676440635 22-23 25.66707362753734 28.505953182573833 27.357705358857803 18.469267831031026 24-25 23.24580724622958 30.363769532333073 27.698311896547416 18.692111324889932 26-27 23.330485020400786 30.137013978953554 27.65818534051891 18.874315660126754 28-29 23.598208196138014 29.851444096811818 27.616458933559016 18.933888773491148 30-31 24.12645269395834 28.41351757854762 28.42324593340027 19.03678379409377 32-33 22.674645100653485 29.602352302094054 28.58446349351837 19.138539103734093 34-35 24.537441303427684 28.478675366288282 27.87152307902562 19.11236025125841 36-37 22.79909683248525 29.12321566323895 28.771184391491328 19.306503112784473 38-39 22.885569351626604 29.967197508862686 27.82381437853833 19.32341876097238 40-41 24.425548793884712 28.35038168951987 27.769125394997413 19.454944121598007 42-43 23.94760548722968 28.304567440856182 28.2053823368217 19.54244473509244 44-45 22.557796760420583 29.20323722018771 28.609448016387223 19.62951800300448 46-47 23.22867389010279 29.288971121250047 27.66935413100854 19.813000857638624 48-49 21.731991011469255 28.866733145693775 28.823111814243322 20.57816402859365 50-51 21.406666763018823 28.21356317845314 29.406230818669865 20.973539239858173 52-53 23.871704893412822 28.261233106762955 27.818474127267546 20.04858787255667 54-55 23.420213406709063 28.202541895689357 28.62607322248436 19.75117147511722 56-57 23.64130018060358 28.062725828198932 28.430229334692285 19.865744656505207 58-59 23.09567547236466 28.36421734001365 27.87668454699746 20.663422640624223 60-61 21.769304043494298 28.218431418679774 27.81334788567918 22.198916652146753 62-63 22.940402137845048 28.3228062893714 28.252470414935964 20.48432115784759 64-65 21.721348021149442 29.127823589382594 28.96360044576959 20.187227943698375 66-67 21.38442073385107 28.955502025650425 28.153349468958673 21.50672777153983 68-69 21.21451724885133 28.3236413111574 30.2463174000911 20.215524039900178 70-71 21.155968527556073 30.015964966684628 28.64020990275991 20.187856602999386 72-73 21.135887415174818 31.002032886365573 27.69661035283562 20.165469345623986 74-75 20.971921475873444 31.097740712857767 27.858184234216445 20.072153577052347 76-77 21.104914316707614 31.02087438668685 27.70403854458523 20.170172752020303 78-79 21.059040748674185 31.11301965735486 27.714204059818826 20.113735534152127 80-81 21.077910034718368 30.89471916122852 27.751935173511505 20.27543563054161 82-83 21.252319708550534 30.82828892871266 27.559744751700876 20.35964661103593 84-85 21.222349942864792 30.570262594918823 27.77152934387906 20.43585811833733 86-87 21.476742907071003 30.195915319806595 27.722896508243938 20.604445264878468 88-89 21.48505007734206 30.27562258845164 27.661724963061445 20.57760237114486 90-91 21.596186756810663 29.95858932763049 27.74034472088741 20.704879194671435 92-93 21.680350447860977 29.93713409653588 27.595956207576865 20.786559248026286 94-95 21.609505318930207 29.88139722269263 27.759528966971885 20.749568491405274 96-97 21.756630951377247 29.703705385121975 27.652601646484083 20.887062017016696 98-99 21.733701216801844 29.78383283367328 27.660610546009146 20.82185540351573 100-101 21.933960873216577 29.66116738695554 27.5954726777761 20.809399062051785 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 756.0 1 532.0 2 251.0 3 198.0 4 200.5 5 247.0 6 352.5 7 522.5 8 748.5 9 941.5 10 1104.5 11 1192.0 12 1185.5 13 1173.0 14 1075.0 15 978.0 16 942.5 17 917.0 18 1004.0 19 1251.0 20 1777.0 21 2604.0 22 3745.5 23 5407.0 24 7751.0 25 10824.5 26 14693.0 27 19489.0 28 25306.0 29 31869.0 30 39281.5 31 47769.0 32 57615.0 33 68609.5 34 80153.0 35 92545.5 36 105999.0 37 119554.0 38 131885.0 39 142156.5 40 151050.5 41 158341.0 42 162497.0 43 163851.0 44 163576.0 45 161766.0 46 157861.0 47 153005.0 48 146334.0 49 137785.0 50 128201.5 51 116720.5 52 103396.0 53 88882.5 54 74845.5 55 62028.0 56 50931.0 57 41760.5 58 33859.0 59 27141.0 60 21728.0 61 17165.0 62 13185.0 63 10082.0 64 7686.0 65 5736.5 66 4351.0 67 3321.5 68 2466.0 69 1893.0 70 1493.5 71 1138.0 72 885.0 73 711.5 74 527.0 75 404.5 76 330.5 77 266.5 78 212.0 79 157.0 80 136.0 81 119.0 82 98.0 83 80.0 84 68.0 85 58.0 86 48.0 87 38.5 88 34.5 89 26.0 90 18.0 91 18.5 92 17.5 93 14.0 94 9.5 95 6.0 96 6.0 97 3.0 98 1.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23461463744941002 2 0.22697342415405158 3 0.20866390152709646 4 0.19826009573264686 5 0.20161047386984252 6 0.12622696578294076 7 0.10221592246637205 8 0.09780753018058831 9 0.09107738462429184 10-11 0.07190087818113261 12-13 0.10009989416919587 14-15 0.1022747010301825 16-17 0.0918708952357329 18-19 0.08680124410708161 20-21 0.08092338772603663 22-23 0.16071528809872213 24-25 0.62835754177466 26-27 0.3611942746152135 28-29 0.1803179391295071 30-31 0.3072649423191259 32-33 0.34414849111018303 34-35 0.3265883951718112 36-37 0.33577254576719395 38-39 0.3438545982911308 40-41 0.3455003980778234 42-43 0.36663129176768006 44-45 0.3428847519882584 46-47 0.3667341542543484 48-49 0.3543759612132013 50-51 0.3603125961580567 52-53 0.35393512198462296 54-55 0.3371097580938817 56-57 0.34529467310448686 58-59 0.33634563676434587 60-61 0.3147004306411477 62-63 0.3029006339562 64-65 0.3016956733980858 66-67 0.31527352163829964 68-69 0.31258440234397156 70-71 0.29897716482185244 72-73 0.2906012194788634 74-75 0.2972725864713494 76-77 0.2987714398485159 78-79 0.301460559142844 80-81 0.3113059685810943 82-83 0.3394462060053471 84-85 0.3257654952784649 86-87 0.3362721635595828 88-89 0.33787387942341757 90-91 0.3186532890574005 92-93 0.2980660970827905 94-95 0.29808079172374313 96-97 0.3057366996600542 98-99 0.3270586236822948 100-101 0.33412674598050135 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3402601.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.72089764988182 #Duplication Level Percentage of deduplicated Percentage of total 1 97.70164008957028 92.54387051149776 2 1.6636955171751955 3.1517346560583777 3 0.2972843196467946 0.8447711284253635 4 0.11161364382102801 0.4228857813080783 5 0.04469830873150696 0.21169319632399447 6 0.046067413366511455 0.2618128047890478 7 0.03200295428703474 0.21219439902612452 8 0.02803569504286166 0.21244529605585588 9 0.007338256496067389 0.06255776182433215 >10 0.06454053625087702 1.0530981376916864 >50 0.0028951642993601183 0.18839331046905086 >100 1.571186996043681E-4 0.031608848373070916 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 3.0982612916688054E-5 0.8029341681572562 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT 27360 0.8040907529269521 Illumina Single End PCR Primer 1 (97% over 34bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.003703049520058332 0.0 0.0 2 0.004525949413404628 0.0 0.0 3 0.006700756274391267 0.0 2.9389281905224857E-5 4 0.009962966565871227 0.0 5.8778563810449715E-5 5 0.012725559064962363 0.0 5.8778563810449715E-5 6 0.022717914912738814 0.0 8.816784571567457E-5 7 0.032416377941463015 0.0 8.816784571567457E-5 8 0.04132133035874615 0.0 8.816784571567457E-5 9 0.04602361546358212 0.0 1.1755712762089943E-4 10-11 0.055207766058964894 0.0 1.1755712762089943E-4 12-13 0.06737492876772798 0.0 1.469464095261243E-4 14-15 0.07939514506696495 0.0 1.7633569143134914E-4 16-17 0.09314932899861017 0.0 2.2041961428918643E-4 18-19 0.11012163929887753 0.0 2.498088961944113E-4 20-21 0.1309880294515872 0.0 3.2328210095747343E-4 22-23 0.15455823353957754 0.0 3.2328210095747343E-4 24-25 0.17626221822658605 0.0 3.2328210095747343E-4 26-27 0.1977751725812107 0.0 3.2328210095747343E-4 28-29 0.22128659810539056 0.0 3.2328210095747343E-4 30-31 0.2513665281353882 0.0 3.3797674191008586E-4 32-33 0.2860311861426009 0.0 3.8206066476792315E-4 34-35 0.3280578592670724 0.0 4.4083922857837286E-4 36-37 0.37712326540784535 0.0 4.555338695309853E-4 38-39 0.4338298848439767 0.0 4.702285104835977E-4 40-41 0.498163022934514 0.0 4.996177923888226E-4 42-43 0.5672425300527449 0.0 5.290070742940474E-4 44-45 0.6381441726491 0.0 5.437017152466598E-4 46-47 0.7144387484750636 0.0 5.583963561992723E-4 48-49 0.7989329339525851 0.0 5.583963561992723E-4 50-51 0.8863954369025343 0.0 5.583963561992723E-4 52-53 0.9822338851954725 0.0 6.17174920009722E-4 54-55 1.0815255741122747 0.0 6.17174920009722E-4 56-57 1.1844027554215142 0.0 6.465642019149469E-4 58-59 1.2941570286965765 0.0 6.759534838201717E-4 60-61 1.4060714141916728 0.0 6.759534838201717E-4 62-63 1.5197050726782249 0.0 6.759534838201717E-4 64-65 1.6373944520676975 0.0 6.759534838201717E-4 66-67 1.757449668650541 0.0 6.759534838201717E-4 68-69 1.8809581258572488 0.0 7.20037406678009E-4 70-71 2.0097566538068965 0.0 7.347320476306214E-4 72-73 2.145211854108078 0.0 7.494266885832339E-4 74-75 2.287132696428409 0.0 7.641213295358463E-4 76-77 2.4327418936278455 0.0 7.788159704884587E-4 78-79 2.58368524549308 0.0 7.935106114410712E-4 80-81 2.746222669069926 0.0 7.935106114410712E-4 82-83 2.911699020837295 0.0 7.935106114410712E-4 84-85 3.0841994109800117 0.0 8.669838162041333E-4 86-87 3.2656047535400123 0.0 9.110677390619706E-4 88-89 3.456076689567775 0.0 9.404570209671954E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 1845 0.0 74.79923 2 GAGCGGC 1185 0.0 69.96061 9 GATCGGG 2025 0.0 69.573 1 TCGGGAG 1990 0.0 60.0513 3 AAGCGGC 400 0.0 56.84799 9 CGAGCCG 35 2.807314E-4 54.212723 1 CGGGAGA 2160 0.0 53.567112 4 AGAGCGG 1580 0.0 53.370735 8 GATCCGG 385 0.0 49.284294 1 GAGAGCG 2285 0.0 48.929684 7 GTTCGGA 265 0.0 42.950268 2 TCCGGAG 345 0.0 41.236053 3 GCGTCGG 565 0.0 40.665596 10-11 AGCGGCG 1160 0.0 40.226486 10-11 TCGGGAA 720 0.0 40.176517 3 CGTCGGG 595 0.0 39.809517 12-13 CGCCGGA 975 0.0 37.750412 58-59 AAAGCGG 660 0.0 37.32499 8 GGCGCCG 1555 0.0 37.264534 56-57 CGCCGTA 4390 0.0 36.944584 58-59 >>END_MODULE