FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n02_34211-2.34200000035860.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n02_34211-2.34200000035860.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3402601
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT273600.8040907529269521Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA18450.074.799232
GAGCGGC11850.069.960619
GATCGGG20250.069.5731
TCGGGAG19900.060.05133
AAGCGGC4000.056.847999
CGAGCCG352.807314E-454.2127231
CGGGAGA21600.053.5671124
AGAGCGG15800.053.3707358
GATCCGG3850.049.2842941
GAGAGCG22850.048.9296847
GTTCGGA2650.042.9502682
TCCGGAG3450.041.2360533
GCGTCGG5650.040.66559610-11
AGCGGCG11600.040.22648610-11
TCGGGAA7200.040.1765173
CGTCGGG5950.039.80951712-13
CGCCGGA9750.037.75041258-59
AAAGCGG6600.037.324998
GGCGCCG15550.037.26453456-57
CGCCGTA43900.036.94458458-59
Produced by FastQC (version 0.11.2)