FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n02_34211-1.34200000035897.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n02_34211-1.34200000035897.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3702924
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT101340.27367561419029934Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA11100.062.415752
GATCGGG13200.056.80681
GAGCGGC8450.049.3517659
TCGGGAG14600.041.9243053
AGCGGCG6950.034.4336710-11
CGCCGTA14650.034.357258-59
CGTCGGG3200.034.06078312-13
TCGCCGT13700.033.79810756-57
GTCGCCG15450.033.6584456-57
AAGCGGC3300.033.0286379
GCGTCGG3300.033.02863710-11
GCCGTAT15350.032.9450958-59
TTGTGTT313850.032.3297841
GTGTTTG704900.031.8255041
CGCCGGA2350.031.31939358-59
TGTTTGA717150.031.2052782
GGCGCCG5850.030.84864256-57
GTTTGAT168050.030.8045753
AGAGCGG13850.030.4529068
TGGTCGC15650.030.35020854-55
Produced by FastQC (version 0.11.2)