##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n02_34211-1.34200000035897.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3702924 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06830223898735 33.0 33.0 33.0 33.0 33.0 2 32.01594415656384 33.0 33.0 33.0 33.0 33.0 3 31.98439422467218 33.0 33.0 33.0 33.0 33.0 4 35.71081069986854 37.0 37.0 37.0 33.0 37.0 5 35.70790029717056 37.0 37.0 37.0 33.0 37.0 6 35.682551410722986 37.0 37.0 37.0 33.0 37.0 7 35.58994918610266 37.0 37.0 37.0 33.0 37.0 8 35.58130952728168 37.0 37.0 37.0 33.0 37.0 9 35.52471911386785 37.0 37.0 37.0 33.0 37.0 10-11 35.400656211145574 37.0 37.0 37.0 33.0 37.0 12-13 35.39101531654444 37.0 37.0 37.0 33.0 37.0 14-15 37.05082442955891 40.0 37.0 40.0 33.0 40.0 16-17 36.991542899611225 40.0 37.0 40.0 33.0 40.0 18-19 36.95496302381578 40.0 37.0 40.0 33.0 40.0 20-21 36.93828633803989 40.0 37.0 40.0 33.0 40.0 22-23 36.93489590928682 40.0 37.0 40.0 33.0 40.0 24-25 36.61081364348823 40.0 37.0 40.0 33.0 40.0 26-27 36.5470547059567 40.0 37.0 40.0 33.0 40.0 28-29 36.51614831954423 40.0 37.0 40.0 33.0 40.0 30-31 36.46341499312435 40.0 37.0 40.0 33.0 40.0 32-33 36.38349679874607 40.0 37.0 40.0 33.0 40.0 34-35 36.293769329319204 40.0 37.0 40.0 33.0 40.0 36-37 36.1777442637224 37.0 37.0 40.0 33.0 40.0 38-39 36.07433004296064 37.0 37.0 40.0 30.0 40.0 40-41 35.9003748119054 37.0 37.0 40.0 27.0 40.0 42-43 35.81718946972717 37.0 37.0 40.0 27.0 40.0 44-45 35.68174245542171 37.0 37.0 40.0 27.0 40.0 46-47 35.54142699121019 37.0 37.0 40.0 27.0 40.0 48-49 35.37487901453014 37.0 37.0 40.0 27.0 40.0 50-51 34.261441633692726 37.0 35.0 38.5 24.5 38.5 52-53 34.71204580488285 37.0 35.0 38.5 27.0 40.0 54-55 35.42425107833701 37.0 37.0 40.0 27.0 40.0 56-57 35.46149434879031 37.0 37.0 40.0 27.0 40.0 58-59 35.27464984428522 37.0 37.0 40.0 27.0 40.0 60-61 35.049417163301214 37.0 33.0 40.0 27.0 40.0 62-63 34.774817819647396 37.0 33.0 40.0 27.0 40.0 64-65 34.554974123152405 37.0 33.0 40.0 27.0 40.0 66-67 34.32701467813004 37.0 33.0 40.0 27.0 40.0 68-69 34.12795455699334 37.0 33.0 37.0 27.0 40.0 70-71 33.78022192732014 37.0 33.0 37.0 22.0 40.0 72-73 33.50387423560408 37.0 33.0 37.0 22.0 40.0 74-75 33.29168800115801 37.0 33.0 37.0 22.0 40.0 76-77 33.009494118701866 37.0 33.0 37.0 22.0 37.0 78-79 32.70585002554738 37.0 33.0 37.0 22.0 37.0 80-81 32.432351163566956 37.0 33.0 37.0 22.0 37.0 82-83 32.12515177195103 37.0 33.0 37.0 22.0 37.0 84-85 31.90316733478732 35.0 33.0 37.0 22.0 37.0 86-87 31.702383170705097 33.0 33.0 37.0 18.5 37.0 88-89 31.493393599220507 33.0 33.0 37.0 15.0 37.0 90-91 31.28616952440828 33.0 33.0 37.0 15.0 37.0 92-93 31.10486105034832 33.0 33.0 37.0 15.0 37.0 94-95 30.9338726638732 33.0 27.0 37.0 15.0 37.0 96-97 30.70494898085945 33.0 27.0 37.0 15.0 37.0 98-99 30.567389041741066 33.0 27.0 37.0 15.0 37.0 100-101 29.163780839142255 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.2615782563571756 2306 2 0.17100839751058317 2306 3 0.19298401714607394 2306 4 0.2402252139885377 2306 5 0.20181707193080456 2306 6 0.33817454387889967 2306 7 0.33797257653826307 2306 8 0.27852981597889936 2306 9 0.31112514573840144 2306 10-11 0.32742300595803187 2306 12-13 0.34277424646128196 2306 14-15 0.5218736183876587 2306 16-17 0.5190296429866734 2306 18-19 0.5111890454768542 2306 20-21 0.465334880718018 2306 22-23 0.5481677516729491 2306 24-25 0.45310823427259805 2306 26-27 0.4555951228699442 2306 28-29 0.47196483159984126 2306 30-31 0.35888175771669495 2306 32-33 0.33669433817976824 2306 34-35 0.42957191710429043 2306 36-37 0.3513459891286388 2306 38-39 0.3409597646711049 2306 40-41 0.5664407173016102 2306 42-43 0.36247797684490735 2306 44-45 0.6315269078957257 2306 46-47 0.5379164007172008 2306 48-49 0.48831831893958366 2306 50-51 0.2971559386511231 2306 52-53 0.46234414670136204 2306 54-55 0.452466314446788 2306 56-57 0.35066842482887495 2306 58-59 0.42252472068477687 2306 60-61 0.42755922289631343 2306 62-63 0.4551860316790197 2306 64-65 0.38851762870159234 2306 66-67 0.4975453115995805 2306 68-69 0.3706215278596048 2306 70-71 0.4524634833453547 2306 72-73 0.32186048821152013 2306 74-75 0.37106412004302314 2306 76-77 0.45676062781543436 2306 78-79 0.43093403707964484 2306 80-81 0.3914263660875079 2306 82-83 0.4295501098353611 2306 84-85 0.404292731209118 2306 86-87 0.4599616556153876 2306 88-89 0.4106098331719501 2306 90-91 0.45852990897114054 2306 92-93 0.38880951258361307 2306 94-95 0.39717640866076565 2306 96-97 0.50061017725643 2306 98-99 0.5071984479595173 2306 100-101 0.45500222403381585 2307 1 0.08611220048231871 2307 2 0.008957788693848556 2307 3 0.007317133221206973 2307 4 0.05575578073618459 2307 5 0.05207093073271807 2307 6 0.18020234058447926 2307 7 0.24602754780092795 2307 8 0.18274764942086819 2307 9 0.17276469216253076 2307 10-11 0.18393528968736916 2307 12-13 0.19740110550021228 2307 14-15 0.33054416484140603 2307 16-17 0.33401527431926326 2307 18-19 0.3442266113949728 2307 20-21 0.3446617753082819 2307 22-23 0.34452068391801305 2307 24-25 0.29204880427911917 2307 26-27 0.29708307614955487 2307 28-29 0.3685074635841943 2307 30-31 0.3547011420859576 2307 32-33 0.31142751795105283 2307 34-35 0.2709278489938427 2307 36-37 0.20885621013947997 2307 38-39 0.2756705582066843 2307 40-41 0.4330632842566331 2307 42-43 0.36994292544360263 2307 44-45 0.3648455516120279 2307 46-47 0.2581390039642244 2307 48-49 0.36019544831684414 2307 50-51 0.23989726190170302 2307 52-53 0.38794518657811494 2307 54-55 0.3337057601421165 2307 56-57 0.3512497448205778 2307 58-59 0.3841928748294663 2307 60-61 0.38604923519115886 2307 62-63 0.43454014526555795 2307 64-65 0.42249665711303663 2307 66-67 0.2771800400762032 2307 68-69 0.2398308252762078 2307 70-71 0.28278220483021244 2307 72-73 0.23159229777483148 2307 74-75 0.26408235491889087 2307 76-77 0.19842957816205598 2307 78-79 0.2596525778451877 2307 80-81 0.21440725353788537 2307 82-83 0.10329377725255995 2307 84-85 0.21245287519205647 2307 86-87 0.13614133061686573 2307 88-89 0.2811190691143608 2307 90-91 0.12365626436605837 2307 92-93 0.27670066992792997 2307 94-95 0.25717587168736955 2307 96-97 0.1980772367160526 2307 98-99 0.15238809214983462 2307 100-101 0.21798050068250774 2201 1 0.2365926346536611 2201 2 0.18975944958105373 2201 3 0.17587033742437796 2201 4 0.27355515731700564 2201 5 0.31488834619855766 2201 6 0.37423496077936136 2201 7 0.4554095925858448 2201 8 0.3837175754896194 2201 9 0.3851120018712706 2201 10-11 0.41031335917316625 2201 12-13 0.3827374941327051 2201 14-15 0.6855040681127136 2201 16-17 0.5898452080186587 2201 18-19 0.5873336981533228 2201 20-21 0.5851811381526417 2201 22-23 0.5261256478125489 2201 24-25 0.5980582959940648 2201 26-27 0.580840781325783 2201 28-29 0.661460959980495 2201 30-31 0.5955646496480185 2201 32-33 0.5349987512647232 2201 34-35 0.5257457121329097 2201 36-37 0.6143187194619344 2201 38-39 0.561491095329842 2201 40-41 0.6102662910307046 2201 42-43 0.5997011069649929 2201 44-45 0.5717026738030313 2201 46-47 0.5862797297487319 2201 48-49 0.5608212296678943 2201 50-51 0.6331169348917243 2201 52-53 0.6433963574521187 2201 54-55 0.7386473687616828 2201 56-57 0.6717738352766318 2201 58-59 0.7639743685918603 2201 60-61 0.6366541968049759 2201 62-63 0.7357721752094903 2201 64-65 0.7576910870676556 2201 66-67 0.7916759857663465 2201 68-69 0.6809961701556446 2201 70-71 0.5969118827954745 2201 72-73 0.5272674290711308 2201 74-75 0.4707618251266297 2201 76-77 0.45564578380241727 2201 78-79 0.5049898038286145 2201 80-81 0.570562581158768 2201 82-83 0.570296448630117 2201 84-85 0.6399796630910544 2201 86-87 0.5015987474120394 2201 88-89 0.5101079955555186 2201 90-91 0.5192147367121365 2201 92-93 0.5075817814086605 2201 94-95 0.5413880922540777 2201 96-97 0.6081196809597174 2201 98-99 0.401258808749553 2201 100-101 0.3244027542773331 1101 1 0.23004144470514376 1101 2 0.159194251461102 1101 3 0.2047690663612869 1101 4 0.2932720682050842 1101 5 0.2319522475094118 1101 6 0.2976143882524056 1101 7 0.2823410921009142 1101 8 0.2795748079980598 1101 9 0.3166044952994582 1101 10-11 0.2880603928960497 1101 12-13 0.26578901102633523 1101 14-15 0.4567927291884004 1101 16-17 0.41183025485153024 1101 18-19 0.3541858531384676 1101 20-21 0.38438249976563554 1101 22-23 0.48834673877057355 1101 24-25 0.5068193276485005 1101 26-27 0.6190997949901842 1101 28-29 0.6758887475349269 1101 30-31 0.6287623775623956 1101 32-33 0.5014963134391479 1101 34-35 0.5517333130872757 1101 36-37 0.6454401631110827 1101 38-39 0.5472259757644764 1101 40-41 0.7656904346466433 1101 42-43 0.5911146654589103 1101 44-45 0.5619621965363208 1101 46-47 0.5882397912466004 1101 48-49 0.6288273472715886 1101 50-51 0.5434178267862606 1101 52-53 0.5896536040038356 1101 54-55 0.6952062386287494 1101 56-57 0.7041454965234735 1101 58-59 0.6199197226799882 1101 60-61 0.6834505004967397 1101 62-63 0.7094699170208685 1101 64-65 0.6815489368952612 1101 66-67 0.5554459334404953 1101 68-69 0.6490196224326255 1101 70-71 0.5986139721721742 1101 72-73 0.521669280443497 1101 74-75 0.4204805206814868 1101 76-77 0.4768765163496198 1101 78-79 0.5440521369732423 1101 80-81 0.6682906418257346 1101 82-83 0.6942722766350471 1101 84-85 0.6611180171894304 1101 86-87 0.7138930535935728 1101 88-89 0.7487391561301884 1101 90-91 0.6645188688008865 1101 92-93 0.6643428159887748 1101 94-95 0.5646731664420876 1101 96-97 0.8349190858757467 1101 98-99 0.7758661454854519 1101 100-101 0.8887952873485254 2304 1 0.13068313437483425 2304 2 0.035415807044600456 2304 3 -0.018443533509774568 2304 4 -0.008699306248281857 2304 5 0.030763474982911987 2304 6 0.176634229736095 2304 7 0.13522510295075563 2304 8 0.21250097276958968 2304 9 0.07305941435474494 2304 10-11 0.14224215374040483 2304 12-13 0.24159461598101473 2304 14-15 0.32039485365024944 2304 16-17 0.3220390085764322 2304 18-19 0.3799215009456489 2304 20-21 0.3133214065180425 2304 22-23 0.304408740163737 2304 24-25 0.3234803059308433 2304 26-27 0.39690571530091034 2304 28-29 0.3925002268940787 2304 30-31 0.26667376251432984 2304 32-33 0.2400697464888566 2304 34-35 0.24439010791223126 2304 36-37 0.33566692007336485 2304 38-39 0.2819684316695259 2304 40-41 0.41511411149851085 2304 42-43 0.37351340722821647 2304 44-45 0.45696679714188093 2304 46-47 0.44356940816884105 2304 48-49 0.39822563596607097 2304 50-51 0.46025444640900304 2304 52-53 0.40062280489561175 2304 54-55 0.48165610799550507 2304 56-57 0.39283359542551466 2304 58-59 0.31566281528488815 2304 60-61 0.4385206472570573 2304 62-63 0.4352029701264257 2304 64-65 0.3513274491731906 2304 66-67 0.42386282614559434 2304 68-69 0.4116866563493673 2304 70-71 0.35142014197343485 2304 72-73 0.3237881537042995 2304 74-75 0.45135267973271453 2304 76-77 0.5315327084054431 2304 78-79 0.4402524958404328 2304 80-81 0.45359804411027227 2304 82-83 0.5470479087941769 2304 84-85 0.5141147977634972 2304 86-87 0.39493316967653413 2304 88-89 0.45142527108345476 2304 90-91 0.4588252950784266 2304 92-93 0.4790002854361646 2304 94-95 0.5165171011274587 2304 96-97 0.4885278168471032 2304 98-99 0.49823367099835636 2304 100-101 0.5701962665490008 2202 1 0.16027460038345964 2202 2 0.12897753249294652 2202 3 0.12107233059116851 2202 4 0.17111393445812695 2202 5 0.15914064432560338 2202 6 0.2144884660490547 2202 7 0.21824245672571152 2202 8 0.16301170520060282 2202 9 0.16733725139567923 2202 10-11 0.2466584611732614 2202 12-13 0.26708278793672235 2202 14-15 0.3594396692838373 2202 16-17 0.38380994638297494 2202 18-19 0.40182265096504466 2202 20-21 0.4037038840515095 2202 22-23 0.473240898322544 2202 24-25 0.4248991420459234 2202 26-27 0.4398356809886934 2202 28-29 0.4015097371508318 2202 30-31 0.3636697305656327 2202 32-33 0.276498344411948 2202 34-35 0.40813119231082595 2202 36-37 0.31568726165388483 2202 38-39 0.3945878837831387 2202 40-41 0.4807686286741415 2202 42-43 0.41466173270269024 2202 44-45 0.39301447975910264 2202 46-47 0.37816849622905835 2202 48-49 0.36077313257792554 2202 50-51 0.3280157476998724 2202 52-53 0.36934841019235165 2202 54-55 0.421438094484472 2202 56-57 0.43743235364186006 2202 58-59 0.356906288031027 2202 60-61 0.4694357175337416 2202 62-63 0.5576265179343949 2202 64-65 0.5050932331993963 2202 66-67 0.42737366985360126 2202 68-69 0.5358114977856872 2202 70-71 0.5995852494934795 2202 72-73 0.5688678919508305 2202 74-75 0.45924621821202294 2202 76-77 0.538239352633056 2202 78-79 0.600808412049453 2202 80-81 0.5540375580533023 2202 82-83 0.5122064277734282 2202 84-85 0.4200051448522828 2202 86-87 0.45702386272684237 2202 88-89 0.4557371642024357 2202 90-91 0.4425321554564796 2202 92-93 0.58057353198069 2202 94-95 0.5464216167626041 2202 96-97 0.5869337164370663 2202 98-99 0.7550876877778663 2202 100-101 0.44630569883936033 1102 1 0.03470534128707925 1102 2 0.029553933506612395 1102 3 0.039285238802293065 1102 4 -0.010812602131913707 1102 5 -0.01813951081273757 1102 6 0.05964636351556152 1102 7 -0.02432597415370452 1102 8 0.15234999922733294 1102 9 0.06693688783821017 1102 10-11 0.15095559566636751 1102 12-13 0.07532676415501527 1102 14-15 0.14675972204811671 1102 16-17 0.19827670154503352 1102 18-19 0.26637657646905666 1102 20-21 0.1949746949902078 1102 22-23 0.21813102591448086 1102 24-25 0.10291664611951035 1102 26-27 0.22586634689284324 1102 28-29 0.18686137399717495 1102 30-31 0.2139703954122325 1102 32-33 0.27685642739222516 1102 34-35 0.24805193276944237 1102 36-37 0.1950911994248159 1102 38-39 0.24497600176707834 1102 40-41 0.3266362358293122 1102 42-43 0.2184310856444469 1102 44-45 0.33587962168557084 1102 46-47 0.2881526458147974 1102 48-49 0.2700367942359847 1102 50-51 0.2167063122480286 1102 52-53 0.35780491620953825 1102 54-55 0.333304540791282 1102 56-57 0.48249382321953505 1102 58-59 0.4884883104832767 1102 60-61 0.4524727969169646 1102 62-63 0.5336415212430978 1102 64-65 0.4801617790644812 1102 66-67 0.4040789606777011 1102 68-69 0.5107011355546902 1102 70-71 0.4300416823040152 1102 72-73 0.5247785448314559 1102 74-75 0.5911608720603354 1102 76-77 0.5882144719753697 1102 78-79 0.5708383979641098 1102 80-81 0.5900422121182558 1102 82-83 0.5607053018743144 1102 84-85 0.5565064196191614 1102 86-87 0.5571330537581076 1102 88-89 0.5578330728149261 1102 90-91 0.46576123476116393 1102 92-93 0.5577662446532159 1102 94-95 0.6663412110995672 1102 96-97 0.7239570955046375 1102 98-99 0.575547944195371 1102 100-101 0.6105787071515181 2305 1 0.11893453071745341 2305 2 0.08434882709878622 2305 3 0.12905417474647862 2305 4 0.06536660951471873 2305 5 0.05883018411518037 2305 6 0.24455534797989742 2305 7 0.14102184784820793 2305 8 0.1416012233674806 2305 9 0.1574038485006497 2305 10-11 0.22825469762046424 2305 12-13 0.17979127747297952 2305 14-15 0.1744592500602593 2305 16-17 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52-53 -0.40255259938508203 1213 54-55 -0.2952517002228987 1213 56-57 -0.9817564114909132 1213 58-59 -0.5812708451811304 1213 60-61 -0.4858073284968114 1213 62-63 -0.40374777013254004 1213 64-65 -0.2993366528283943 1213 66-67 -0.18988500123885643 1213 68-69 -0.3948460991463989 1213 70-71 -0.23101450597235385 1213 72-73 -0.27500189733539315 1213 74-75 -0.27313753891139214 1213 76-77 -0.26016889123547315 1213 78-79 -0.3025347221504333 1213 80-81 -0.25658008882043504 1213 82-83 -0.10909103666458009 1213 84-85 -0.23732696456719538 1213 86-87 -0.35611254198233055 1213 88-89 -0.25271749034876834 1213 90-91 -0.2395514634813516 1213 92-93 -0.24063923581650215 1213 94-95 -0.14422745689993377 1213 96-97 -0.10203808871777298 1213 98-99 -0.17011861170377074 1213 100-101 0.07692891252451162 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1380.0 3 248.0 4 287.0 5 467.0 6 1352.0 7 2819.0 8 7927.0 9 12454.0 10 13260.0 11 13965.0 12 11914.0 13 10626.0 14 10079.0 15 9885.0 16 10320.0 17 11542.0 18 12722.0 19 14454.0 20 16083.0 21 18102.0 22 20925.0 23 23868.0 24 27115.0 25 32116.0 26 38098.0 27 45454.0 28 54516.0 29 64217.0 30 77016.0 31 96105.0 32 126454.0 33 172850.0 34 250486.0 35 388744.0 36 638092.0 37 995107.0 38 469390.0 39 2485.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.28855717352521 24.405903739377845 16.098572523031027 16.206966564065915 2 37.46097975332383 21.69406610730879 28.33411292619096 12.510841213176418 3 43.03175546116258 19.15636320334077 22.341841781564323 15.470039553932322 4 36.03725329474791 19.663571877204628 28.49309849050875 15.806076337538707 5 37.423691917499866 17.474070541981558 28.814835822947387 16.287401717571196 6 33.7979951487684 17.936063255300798 31.569295003015117 16.696646592915688 7 40.2296529333953 18.323410308753267 22.16558487991126 19.281351877940175 8 34.42110467805477 26.42923741654441 20.446738321488635 18.702919583912188 9 33.94666481409347 22.637570454773382 23.057589166123105 20.358175565010047 10-11 29.197825192444256 28.414480969758227 27.71197245477991 14.67572138301761 12-13 29.511928539064858 27.320460942712515 26.688835553556235 16.478774964666393 14-15 27.827828693802886 27.795887244385607 27.394611803250946 16.98167225856056 16-17 27.39560245139932 27.8969193696255 27.656526209355597 17.050951969619582 18-19 26.5887560269358 28.23058287691367 27.769795548767924 17.410865547382613 20-21 25.857826161520432 28.69832437039727 27.69157355173229 17.752275916350012 22-23 26.187394697821887 28.61250462054833 27.192303941664164 18.007796739965617 24-25 24.779479742196262 29.469054052382916 27.548218613242888 18.203247592177938 26-27 24.774604635694004 29.14887051257396 27.559119145768342 18.51740570596369 28-29 24.59324746902848 29.31238440168619 27.35520256497212 18.73916556431321 30-31 24.62981619552282 28.51939752028795 27.984503221881106 18.866283062308124 32-33 23.979661384407965 28.534793124707825 28.435918455837022 19.049627035047187 34-35 23.345323287800767 29.504093839468275 28.087679708762103 19.062903163968848 36-37 23.747441636247217 29.06150830802632 27.815771795985583 19.375278259740885 38-39 23.14786071566248 28.873255372007772 28.593356101599493 19.385527810730263 40-41 23.331552839738233 28.920554755191613 28.16379077655537 19.58410162851478 42-43 23.605873288437067 28.3610467724426 28.21368599808362 19.81939394103672 44-45 22.929827475799378 28.85745907079256 28.346197966391617 19.866515487016446 46-47 23.35200839849367 28.691478689472856 27.78017815627259 20.176334755760887 48-49 22.722628283240027 28.758691886055253 28.078234021239968 20.44044580946475 50-51 22.481301033372862 28.28142978146705 28.591834660342197 20.64543452481789 52-53 23.581085641014106 28.22355491748561 27.815014585595886 20.380344855904394 54-55 23.10249231664305 28.375301450580398 28.29234029978472 20.229865932991835 56-57 23.277038952134532 28.18423689575343 28.177217882764864 20.36150626934717 58-59 22.926778906231267 28.576973921582788 27.84684002237728 20.64940714980867 60-61 22.300206196292045 28.312544379884496 28.030003172459043 21.357246251364415 62-63 22.833822776909894 28.371302795774305 27.947706784606268 20.84716764270953 64-65 22.184604136731302 28.73950549546549 28.377186311375084 20.69870405642812 66-67 22.15560929381747 28.38248975520313 28.020962076235485 21.440938874743914 68-69 21.944168788607058 28.38427480299751 28.74601382695099 20.925542581444446 70-71 21.82820811615967 28.831398476493238 28.37536764446958 20.965025762877513 72-73 21.870818328803594 29.36429185774575 27.728655355335658 21.036234458115 74-75 21.49810148148911 29.56990659906507 28.066003110655814 20.865988808790014 76-77 21.667872726245875 29.298674661737873 27.946655463382175 21.08679714863408 78-79 21.530097763793076 29.668118861658993 27.823592845806473 20.97819052874146 80-81 21.46433645679634 29.49980027120854 27.97216285895138 21.063700413043744 82-83 21.669124953695412 29.570618596790073 27.60978353976531 21.150472909749208 84-85 21.40032819705262 29.573878950208364 27.916230499837365 21.10956235290165 86-87 21.68949561341707 29.10300503150926 27.83118592738238 21.376313427691294 88-89 21.597106885414355 29.43120289820905 27.6484515127447 21.323238703631894 90-91 21.564042643664443 29.13082898413454 27.853318018626634 21.451810353574384 92-93 21.645817021799317 29.11586643821377 27.645387148969352 21.592929391017552 94-95 21.402777753324163 29.180237859806198 27.88902367722598 21.527960709643665 96-97 21.544964647057053 28.879051244633263 27.85297477521943 21.72300933309025 98-99 21.42757084110853 29.1453438639777 27.76440930135267 21.662675993561095 100-101 21.520084434833976 28.918212561171895 27.84758916329307 21.714113840701057 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 824.0 1 580.5 2 285.5 3 244.5 4 246.0 5 261.0 6 330.0 7 472.5 8 715.5 9 975.5 10 1204.5 11 1356.0 12 1443.5 13 1450.0 14 1320.0 15 1144.5 16 983.5 17 894.0 18 894.5 19 1016.5 20 1222.5 21 1589.5 22 2261.5 23 3200.0 24 4469.5 25 6420.0 26 9147.5 27 12773.5 28 17309.0 29 22850.5 30 29641.5 31 37776.0 32 47414.5 33 58628.5 34 71440.5 35 85809.0 36 101863.0 37 118836.0 38 134299.5 39 147684.5 40 160134.5 41 170999.0 42 179072.0 43 183402.0 44 184814.0 45 185974.5 46 185263.5 47 182276.5 48 177573.0 49 168708.0 50 157606.0 51 144061.0 52 127529.0 53 109011.5 54 90616.0 55 74003.0 56 59719.5 57 48436.0 58 39087.5 59 31537.0 60 25134.0 61 19655.5 62 15183.5 63 11702.5 64 9020.0 65 6914.0 66 5324.0 67 4051.0 68 3046.5 69 2387.5 70 1892.0 71 1517.5 72 1234.0 73 960.0 74 738.0 75 613.0 76 501.5 77 395.5 78 332.5 79 281.5 80 225.5 81 196.0 82 162.5 83 121.5 84 115.0 85 108.0 86 82.5 87 66.5 88 60.0 89 48.0 90 33.5 91 33.0 92 27.0 93 19.5 94 17.0 95 10.5 96 6.0 97 6.5 98 6.0 99 3.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24288913302028342 2 0.2346253933378055 3 0.21520830565250598 4 0.20451405429871097 5 0.20810581043521284 6 0.13216582354917358 7 0.10669946237081832 8 0.1026486095852899 9 0.09627526786939186 10-11 0.07633156932197366 12-13 0.10521414968279123 14-15 0.10717206186246328 16-17 0.09634278208248401 18-19 0.09145475305461306 20-21 0.08508141133871502 22-23 0.16534230786265125 24-25 0.6376987483405007 26-27 0.36656166856246575 28-29 0.18000639494626408 30-31 0.30913407890629135 32-33 0.34834633387020636 34-35 0.3293478343060781 36-37 0.3396505032239387 38-39 0.3469285353952714 40-41 0.3493455442239701 42-43 0.3694107683549541 44-45 0.3465639586445739 46-47 0.3697348365777964 48-49 0.3585274772045011 50-51 0.3659000292741628 52-53 0.35985075578110703 54-55 0.34141937560695274 56-57 0.349629103918957 58-59 0.3395559833256097 60-61 0.3181539777754013 62-63 0.3056233398255001 64-65 0.3048806834814865 66-67 0.3181809834606381 68-69 0.31669567077261107 70-71 0.30186954957757706 72-73 0.2928631535510856 74-75 0.2997090947586286 76-77 0.3010998875483267 78-79 0.3043810783046047 80-81 0.315115838186255 82-83 0.34256711722951916 84-85 0.32821359552613016 86-87 0.33973152027964926 88-89 0.3414868898200449 90-91 0.32186725949546896 92-93 0.30069480226977385 94-95 0.30050576247311583 96-97 0.3088100106834491 98-99 0.3304145588729339 100-101 0.3376790882016482 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3702924.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.31089978415696 #Duplication Level Percentage of deduplicated Percentage of total 1 97.11906867144417 91.59386752603228 2 2.1880166392816123 4.127076359867121 3 0.30440517133330763 0.861261768221841 4 0.12586911895648437 0.47483319455300504 5 0.06366607116796155 0.3002202228786313 6 0.053112636403263684 0.30054603174603406 7 0.03038300289193512 0.200581383861813 8 0.02162384058216074 0.1631491089674198 9 0.01480156029387232 0.12563536225720942 >10 0.07390291498553 1.1583314781572553 >50 0.004570136786684273 0.29522191380996454 >100 4.7734800430562964E-4 0.07677554510168147 >500 7.421004789353051E-5 0.048412790173839496 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 2.8677820838749473E-5 0.2740873143719137 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT 10134 0.27367561419029934 Illumina Single End PCR Primer 1 (96% over 32bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 8.64181927579394E-4 0.0 0.0 2 0.0010262160390005304 0.0 0.0 3 0.001728363855158788 0.0 0.0 4 0.002133449133711629 0.0 8.101705571056818E-5 5 0.0027815855793961743 0.0 8.101705571056818E-5 6 0.004374921008370682 0.0 8.101705571056818E-5 7 0.007021478161582577 0.0 8.101705571056818E-5 8 0.009911086481926176 0.0 1.0802274094742426E-4 9 0.01145041054042697 0.0 1.350284261842803E-4 10-11 0.016041377030692503 0.0 1.350284261842803E-4 12-13 0.020902400373326593 0.0 1.350284261842803E-4 14-15 0.024642687778631156 0.0 1.4853126880270834E-4 16-17 0.027680827367777466 0.0 1.7553695403956443E-4 18-19 0.03189371426472701 0.0 1.8903979665799245E-4 20-21 0.037362365525190365 0.0 2.160454818948485E-4 22-23 0.04345214754610141 0.0 2.160454818948485E-4 24-25 0.050041534743894285 0.0 2.160454818948485E-4 26-27 0.0557802428567262 0.0 2.160454818948485E-4 28-29 0.061870024877637236 0.0 2.4305116713170457E-4 30-31 0.09042853701561253 0.0 2.700568523685606E-4 32-33 0.09904335060616962 0.0 2.970625376054167E-4 34-35 0.10514663546969907 0.0 3.2406822284227274E-4 36-37 0.11276223870649249 0.0 3.510739080791288E-4 38-39 0.12102597838897045 0.0 3.510739080791288E-4 40-41 0.13072101938900177 0.0 3.780795933159849E-4 42-43 0.14186086454920488 0.0 3.780795933159849E-4 44-45 0.15432398828601396 0.0 3.9158243593441294E-4 46-47 0.16785383658967887 0.0 4.18588121171269E-4 48-49 0.18362515676800278 0.0 4.32090963789697E-4 50-51 0.20234009663714406 0.0 4.590966490265531E-4 52-53 0.22267537762049666 0.0 4.8610233426340914E-4 54-55 0.24515761058017935 0.0 4.996051768818372E-4 56-57 0.2710695655649427 0.0 5.671193899739773E-4 58-59 0.2993310151653126 0.0 5.671193899739773E-4 60-61 0.33033354181722335 0.0 5.671193899739773E-4 62-63 0.36421217394685934 0.0 6.076279178292614E-4 64-65 0.4010344257673125 0.0 6.211307604476894E-4 66-67 0.43977408123958256 0.0 6.211307604476894E-4 68-69 0.4811737967076829 0.0 6.616392883029735E-4 70-71 0.5256791659780218 0.0 7.156506587766856E-4 72-73 0.5753696268138369 0.0 7.291535013951137E-4 74-75 0.6276256277471532 0.0 7.831648718688259E-4 76-77 0.6832708421776952 0.0 8.101705571056819E-4 78-79 0.7447492846193982 0.0 8.101705571056819E-4 80-81 0.811088750403735 0.0 8.37176242342538E-4 82-83 0.8810064694819553 0.0 8.37176242342538E-4 84-85 0.955380126624257 0.0 8.37176242342538E-4 86-87 1.0349793838598902 0.0 8.50679084960966E-4 88-89 1.1220997244339879 0.0 8.64181927579394E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 1110 0.0 62.41575 2 GATCGGG 1320 0.0 56.8068 1 GAGCGGC 845 0.0 49.351765 9 TCGGGAG 1460 0.0 41.924305 3 AGCGGCG 695 0.0 34.43367 10-11 CGCCGTA 1465 0.0 34.3572 58-59 CGTCGGG 320 0.0 34.060783 12-13 TCGCCGT 1370 0.0 33.798107 56-57 GTCGCCG 1545 0.0 33.65844 56-57 AAGCGGC 330 0.0 33.028637 9 GCGTCGG 330 0.0 33.028637 10-11 GCCGTAT 1535 0.0 32.94509 58-59 TTGTGTT 31385 0.0 32.329784 1 GTGTTTG 70490 0.0 31.825504 1 CGCCGGA 235 0.0 31.319393 58-59 TGTTTGA 71715 0.0 31.205278 2 GGCGCCG 585 0.0 30.848642 56-57 GTTTGAT 16805 0.0 30.804575 3 AGAGCGG 1385 0.0 30.452906 8 TGGTCGC 1565 0.0 30.350208 54-55 >>END_MODULE