##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n02_34139-1.342000000356e9.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2741610 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.289078315296486 33.0 33.0 33.0 27.0 33.0 2 30.862352778112133 33.0 33.0 33.0 27.0 33.0 3 30.7345680093084 33.0 33.0 33.0 27.0 33.0 4 34.13869697002856 37.0 37.0 37.0 27.0 37.0 5 34.21095159413629 37.0 37.0 37.0 27.0 37.0 6 34.73502102779024 37.0 37.0 37.0 27.0 37.0 7 34.1486626471307 37.0 37.0 37.0 27.0 37.0 8 34.464012022132984 37.0 37.0 37.0 27.0 37.0 9 33.95261871673944 37.0 37.0 37.0 27.0 37.0 10-11 33.94175630377771 37.0 37.0 37.0 27.0 37.0 12-13 33.94565291927006 37.0 37.0 37.0 27.0 37.0 14-15 35.500501712497396 40.0 37.0 40.0 27.0 40.0 16-17 35.40680968482023 40.0 37.0 40.0 24.5 40.0 18-19 35.443892092602525 40.0 37.0 40.0 24.5 40.0 20-21 35.44278526121512 40.0 37.0 40.0 24.5 40.0 22-23 36.165406093499804 40.0 37.0 40.0 27.0 40.0 24-25 35.40133115213324 40.0 37.0 40.0 22.0 40.0 26-27 35.32221067183151 40.0 37.0 40.0 24.5 40.0 28-29 35.074869328606184 40.0 37.0 40.0 18.5 40.0 30-31 35.03169798038379 40.0 37.0 40.0 18.5 40.0 32-33 34.83440332505353 38.5 37.0 40.0 15.0 40.0 34-35 35.10368870845963 37.0 37.0 40.0 22.0 40.0 36-37 34.71678539252483 37.0 37.0 40.0 15.0 40.0 38-39 34.64495059472354 37.0 37.0 40.0 15.0 40.0 40-41 35.11398229507479 37.0 37.0 40.0 22.0 40.0 42-43 34.76042562581841 37.0 37.0 40.0 18.5 40.0 44-45 34.32448597721776 37.0 37.0 40.0 15.0 40.0 46-47 34.189741064556955 37.0 35.0 40.0 15.0 40.0 48-49 34.070109534178826 37.0 33.0 40.0 15.0 40.0 50-51 33.11880081412017 37.0 33.0 38.5 15.0 38.5 52-53 33.599230379229724 37.0 33.0 38.5 18.5 40.0 54-55 34.22491255138404 37.0 33.0 40.0 18.5 40.0 56-57 34.52324455338287 37.0 33.0 40.0 22.0 40.0 58-59 34.01858925959564 37.0 33.0 40.0 15.0 40.0 60-61 33.78391711439629 37.0 33.0 40.0 15.0 40.0 62-63 33.377180379412096 37.0 33.0 40.0 15.0 40.0 64-65 33.148905570084736 37.0 33.0 38.5 15.0 40.0 66-67 33.047510951594134 37.0 33.0 37.0 15.0 40.0 68-69 33.13841976065159 37.0 33.0 37.0 18.5 40.0 70-71 32.69500330098008 37.0 33.0 37.0 15.0 40.0 72-73 32.867375739073026 37.0 33.0 37.0 22.0 38.5 74-75 32.932873202242476 37.0 33.0 37.0 22.0 37.0 76-77 32.630512910297234 37.0 33.0 37.0 22.0 37.0 78-79 32.1604017347471 37.0 33.0 37.0 22.0 37.0 80-81 31.72180087612753 37.0 33.0 37.0 15.0 37.0 82-83 31.328514449538773 35.0 33.0 37.0 15.0 37.0 84-85 31.07415606158425 33.0 33.0 37.0 15.0 37.0 86-87 30.887562417703464 33.0 33.0 37.0 15.0 37.0 88-89 30.735174769569703 33.0 33.0 37.0 15.0 37.0 90-91 30.57489394917585 33.0 30.0 37.0 15.0 37.0 92-93 30.41934957926182 33.0 27.0 37.0 15.0 37.0 94-95 30.29221260500217 33.0 27.0 37.0 15.0 37.0 96-97 30.097330765499105 33.0 27.0 37.0 15.0 37.0 98-99 29.98144083221173 33.0 27.0 37.0 15.0 37.0 100-101 28.76418947260916 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.037510926503504294 2306 2 -0.022301952387405066 2306 3 -0.10769296683739427 2306 4 0.17631861140901606 2306 5 0.0783027816684978 2306 6 -0.043334956583045425 2306 7 0.552321329131054 2306 8 -0.14829690524693717 2306 9 0.24819155011233107 2306 10-11 0.2427417541612158 2306 12-13 0.26099770041112436 2306 14-15 0.595154172117347 2306 16-17 0.5709454119392348 2306 18-19 0.512170149734267 2306 20-21 0.4769552186446475 2306 22-23 0.4079371077928755 2306 24-25 0.6394394486074404 2306 26-27 0.1879667452301419 2306 28-29 0.35836614341677375 2306 30-31 0.5885885646244731 2306 32-33 0.6463548751026522 2306 34-35 0.34847106809399264 2306 36-37 0.557640468629053 2306 38-39 0.4217336254932178 2306 40-41 0.3301512922975647 2306 42-43 0.6057911614424043 2306 44-45 0.7661576238311909 2306 46-47 0.7698717920530811 2306 48-49 0.5271105511006127 2306 50-51 0.37785290601237875 2306 52-53 0.4065379384380208 2306 54-55 0.6329206118174255 2306 56-57 0.5354396541565904 2306 58-59 0.6969650561972642 2306 60-61 0.5668309212306966 2306 62-63 0.6923980594562593 2306 64-65 0.7251261444690584 2306 66-67 0.5256235245780019 2306 68-69 0.38149475629558083 2306 70-71 0.616211878025716 2306 72-73 0.2336373488887844 2306 74-75 0.2378637684125522 2306 76-77 0.4925658724060682 2306 78-79 0.6548179437386779 2306 80-81 0.6901359520001904 2306 82-83 0.7479237347382863 2306 84-85 0.6945282430631643 2306 86-87 0.6953865413255187 2306 88-89 0.7129081584580774 2306 90-91 0.7105148960885685 2306 92-93 0.708226291628467 2306 94-95 0.6507628960177954 2306 96-97 0.6966652155273714 2306 98-99 0.7120917239238871 2306 100-101 0.49838857074673726 2307 1 -0.18133173324565988 2307 2 -0.21892631567843424 2307 3 -0.16134142685289632 2307 4 -0.12508930235845384 2307 5 -0.17539524731042633 2307 6 -0.16110295637351868 2307 7 0.2391919560765885 2307 8 -0.19785519825092024 2307 9 0.029076845978899257 2307 10-11 0.07145139935026634 2307 12-13 0.029773279111800832 2307 14-15 0.372496402643101 2307 16-17 0.30860797825348385 2307 18-19 0.2587497942539869 2307 20-21 0.2031213745860896 2307 22-23 0.21025394756289728 2307 24-25 0.38817039584025537 2307 26-27 0.13935690729446293 2307 28-29 0.279893187602994 2307 30-31 0.33764350469065363 2307 32-33 0.18766541641319634 2307 34-35 -0.006981808894458652 2307 36-37 0.18944516691315272 2307 38-39 0.06995719412876866 2307 40-41 -0.06468514467370312 2307 42-43 0.3141622050194357 2307 44-45 0.36572697527320486 2307 46-47 0.22663984713573626 2307 48-49 0.25344403867005383 2307 50-51 0.07314758913477704 2307 52-53 0.10104713294720824 2307 54-55 0.1335630197632085 2307 56-57 0.15121155738167857 2307 58-59 0.3364750121866251 2307 60-61 0.31917817170528195 2307 62-63 0.4858157667316405 2307 64-65 0.19014631641807256 2307 66-67 0.23051237272527914 2307 68-69 0.09053051870213835 2307 70-71 0.15421192070741085 2307 72-73 0.045708895042729125 2307 74-75 -0.07297534392468918 2307 76-77 0.3449027434695253 2307 78-79 0.35426681640851854 2307 80-81 0.3281760572687631 2307 82-83 0.4539994248963737 2307 84-85 0.3584186597625738 2307 86-87 0.24709443947536158 2307 88-89 0.2979131105042292 2307 90-91 0.24607723221270206 2307 92-93 0.35936458321619824 2307 94-95 0.33229709908757954 2307 96-97 0.19297494685582706 2307 98-99 0.5498641955914891 2307 100-101 0.4237496918007402 2201 1 0.14156482693903527 2201 2 0.009264282568661741 2201 3 0.1269570846094581 2201 4 0.24249601437940527 2201 5 0.2545516487381647 2201 6 0.21523753013646285 2201 7 0.5347334776068067 2201 8 0.07091792080468196 2201 9 0.33375288229335354 2201 10-11 0.39133179691427955 2201 12-13 0.3994471685596963 2201 14-15 0.6671745009935179 2201 16-17 0.7047090989308842 2201 18-19 0.6629955219460371 2201 20-21 0.5566104568887269 2201 22-23 0.37416209734675476 2201 24-25 0.6483509370942429 2201 26-27 0.5313626679442862 2201 28-29 0.7092504022709392 2201 30-31 0.7030036497118175 2201 32-33 0.73362010643568 2201 34-35 0.42932977430977104 2201 36-37 0.677516897922736 2201 38-39 0.5425388588008246 2201 40-41 0.537033262129448 2201 42-43 0.8804058715455909 2201 44-45 0.9019100510043074 2201 46-47 0.6975811273990473 2201 48-49 0.6484276794164074 2201 50-51 0.5492507022435476 2201 52-53 0.5489503816979209 2201 54-55 0.8654357007884315 2201 56-57 0.844947448857674 2201 58-59 0.871686777624376 2201 60-61 0.7315303326450149 2201 62-63 0.8390224066614493 2201 64-65 0.8582132316601232 2201 66-67 0.7412395275202286 2201 68-69 0.5651903153958244 2201 70-71 0.6965093245867351 2201 72-73 0.5062138371860883 2201 74-75 0.5544002933379559 2201 76-77 0.6059723745956589 2201 78-79 0.6899553950349571 2201 80-81 0.9312645869109559 2201 82-83 1.1447662456066965 2201 84-85 0.9985868592528071 2201 86-87 0.9319776059969485 2201 88-89 0.8306595683919298 2201 90-91 0.7980392333272448 2201 92-93 0.8859103597619722 2201 94-95 0.905784754496139 2201 96-97 0.9770098509472298 2201 98-99 0.9679415515219532 2201 100-101 0.7414395024284062 1101 1 0.09582596744145278 1101 2 0.04491927953971242 1101 3 0.15185336673405558 1101 4 0.18736251597572107 1101 5 0.1860426611863062 1101 6 0.15036009038364284 1101 7 0.4363206081072022 1101 8 0.15321457438747643 1101 9 0.31823946259249425 1101 10-11 0.3032624713574137 1101 12-13 0.29180333329870933 1101 14-15 0.5346521481321815 1101 16-17 0.4575521713153208 1101 18-19 0.5086268960918758 1101 20-21 0.4571518756754074 1101 22-23 0.2976736447865136 1101 24-25 0.5194954614790461 1101 26-27 0.48555265054903174 1101 28-29 0.5434203648556633 1101 30-31 0.568429494300176 1101 32-33 0.5077927835993847 1101 34-35 0.405659942962977 1101 36-37 0.4844119285465851 1101 38-39 0.5261439993351757 1101 40-41 0.40364064718149706 1101 42-43 0.5728656294091437 1101 44-45 0.605959364525205 1101 46-47 0.4670537904908585 1101 48-49 0.5590582501666148 1101 50-51 0.4831889276074932 1101 52-53 0.5585383345560615 1101 54-55 0.6953696227462771 1101 56-57 0.7105099206016021 1101 58-59 0.7657052227893857 1101 60-61 0.6820796855871905 1101 62-63 0.7450889916911407 1101 64-65 0.6828590140327222 1101 66-67 0.6318698387886243 1101 68-69 0.5646213516164948 1101 70-71 0.7059073567165726 1101 72-73 0.4220258605753813 1101 74-75 0.4991239801354226 1101 76-77 0.5858892805344951 1101 78-79 0.6933763633612031 1101 80-81 0.8105345847253318 1101 82-83 0.859049422103233 1101 84-85 0.8516313294458335 1101 86-87 0.6440918139237191 1101 88-89 0.6411537115879682 1101 90-91 0.684052764305882 1101 92-93 0.7970803532849082 1101 94-95 0.8274817256447555 1101 96-97 0.8783305135839434 1101 98-99 0.925122561045935 1101 100-101 0.6458909129615726 2304 1 -0.15605343899603596 2304 2 -0.2835697739419025 2304 3 -0.1646560550972609 2304 4 -0.1973845512978798 2304 5 -0.1749424336198402 2304 6 -0.0791640970316223 2304 7 0.38825494001871874 2304 8 -0.03156647687507075 2304 9 0.09810551056686734 2304 10-11 0.030686356407031212 2304 12-13 0.10306884193799704 2304 14-15 0.26664890036501987 2304 16-17 0.2439012621977028 2304 18-19 0.2104514689039476 2304 20-21 0.16103312275922121 2304 22-23 -0.03787434244284782 2304 24-25 0.26016244777890307 2304 26-27 0.07454569577161863 2304 28-29 0.2556864723479322 2304 30-31 0.10460600162785738 2304 32-33 0.11115251422575767 2304 34-35 0.01598657502108125 2304 36-37 0.3264221090474919 2304 38-39 0.1393477893765862 2304 40-41 0.14520481078960756 2304 42-43 0.473662324328437 2304 44-45 0.40275100042258316 2304 46-47 0.3148838668721652 2304 48-49 0.27247182245331913 2304 50-51 0.09649411852402778 2304 52-53 0.10598604513739218 2304 54-55 0.4234130267295697 2304 56-57 0.37760919426272466 2304 58-59 0.3149533499960455 2304 60-61 0.14170082840444564 2304 62-63 0.3248048671738104 2304 64-65 0.4157642409539264 2304 66-67 0.23811471822812535 2304 68-69 0.11634029851697392 2304 70-71 0.25833344613304376 2304 72-73 0.09795582784053636 2304 74-75 0.2925512011268694 2304 76-77 0.4349023671256944 2304 78-79 0.5452721188528358 2304 80-81 0.6722127692087909 2304 82-83 0.5613070611528599 2304 84-85 0.5061464238478415 2304 86-87 0.5239531776185338 2304 88-89 0.4442970468684919 2304 90-91 0.5136096498294371 2304 92-93 0.5358009755041913 2304 94-95 0.4298402144778031 2304 96-97 0.49606905363177844 2304 98-99 0.48952727177789157 2304 100-101 0.5023040590706813 2202 1 -0.005986468348158525 2202 2 -0.0784829637922968 2202 3 -0.10677576283449497 2202 4 -0.034679719411556675 2202 5 -0.06886334227105806 2202 6 0.23880498831054808 2202 7 0.2274745363989723 2202 8 0.15941041025846658 2202 9 0.1388205353245766 2202 10-11 0.11704473474459576 2202 12-13 0.05277580708945351 2202 14-15 0.18909763839856453 2202 16-17 0.3045238137215662 2202 18-19 0.25756960526285155 2202 20-21 0.15948818916184848 2202 22-23 0.25812979892133114 2202 24-25 0.22742191295247238 2202 26-27 0.32386374478637237 2202 28-29 0.28351832058136495 2202 30-31 0.44469487293605425 2202 32-33 0.2736239352075316 2202 34-35 0.3625279611128249 2202 36-37 0.33269155145307394 2202 38-39 0.2607959471565735 2202 40-41 0.34087023849362197 2202 42-43 0.5092372073083666 2202 44-45 0.3592329190279031 2202 46-47 0.13396881789753934 2202 48-49 0.39203670711346916 2202 50-51 0.34063733299267795 2202 52-53 0.3253193385627 2202 54-55 0.3738589988651526 2202 56-57 0.37379656106120507 2202 58-59 0.3141628990784895 2202 60-61 0.2912865219230909 2202 62-63 0.3341714894654757 2202 64-65 0.3260515480944477 2202 66-67 0.38753621631628477 2202 68-69 0.3455794827063201 2202 70-71 0.31280143253075465 2202 72-73 0.3990184809393753 2202 74-75 0.5386886545552301 2202 76-77 0.5305284763589455 2202 78-79 0.5014430780126489 2202 80-81 0.4982475830616835 2202 82-83 0.39931462145535335 2202 84-85 0.5295691443458956 2202 86-87 0.5654034679833977 2202 88-89 0.3440465956924399 2202 90-91 0.2943608775112736 2202 92-93 0.3490003153892438 2202 94-95 0.22976038709813196 2202 96-97 0.27207825605864144 2202 98-99 0.40782299633636043 2202 100-101 0.2872958824613612 1102 1 -0.06103711103826015 1102 2 -0.16106525488444845 1102 3 -0.1822842498870223 1102 4 -0.15305617806824756 1102 5 -0.2849619678182336 1102 6 0.05769915454794727 1102 7 -0.13135570495819593 1102 8 -0.03608037337994574 1102 9 -0.09926924283646343 1102 10-11 -0.04190999531691375 1102 12-13 -0.010288053285670173 1102 14-15 -0.09202330314158758 1102 16-17 -0.00857083318699381 1102 18-19 0.12135007428604894 1102 20-21 -0.03565699827797886 1102 22-23 0.1611464316739486 1102 24-25 0.07791718262861735 1102 26-27 0.1685591864967435 1102 28-29 0.018710615275303155 1102 30-31 0.06542422520281832 1102 32-33 0.025658624146267073 1102 34-35 0.007291179492277422 1102 36-37 -0.03112549104592688 1102 38-39 0.062271571166434114 1102 40-41 0.1961980680075257 1102 42-43 0.1352223377985169 1102 44-45 0.019922805890871587 1102 46-47 0.04168136148865642 1102 48-49 0.0747590267792333 1102 50-51 0.007018716260184021 1102 52-53 0.08140056624632308 1102 54-55 0.21950612253875335 1102 56-57 0.38355751250243486 1102 58-59 0.25044017602427004 1102 60-61 0.16600793851720397 1102 62-63 0.3564397208802106 1102 64-65 0.26560492885704434 1102 66-67 0.29436698420803964 1102 68-69 0.31916517713786163 1102 70-71 0.20657878740735924 1102 72-73 0.3927806200155004 1102 74-75 0.4915150435062756 1102 76-77 0.4861194805801645 1102 78-79 0.4260906069265289 1102 80-81 0.17416006041930387 1102 82-83 0.260426538451771 1102 84-85 0.35823228658461304 1102 86-87 0.1929989887974095 1102 88-89 0.11976469351368024 1102 90-91 0.12145352074889715 1102 92-93 0.12643952703177064 1102 94-95 0.2319267868452961 1102 96-97 0.24943657396269714 1102 98-99 0.30957830712911516 1102 100-101 0.20624983167145317 2305 1 0.06702177877107118 2305 2 0.0066358749256067995 2305 3 -0.07699249624596405 2305 4 -0.15594964230298558 2305 5 -0.0908857008363313 2305 6 0.30201676487028095 2305 7 -0.13551013305189485 2305 8 0.30264593728221456 2305 9 -0.020296401449492407 2305 10-11 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96-97 -0.38238410401665135 1213 98-99 -0.5129950240607819 1213 100-101 -0.28746358734302646 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 915.0 3 294.0 4 361.0 5 371.0 6 1121.0 7 2912.0 8 12329.0 9 37298.0 10 56280.0 11 45166.0 12 27158.0 13 17956.0 14 13287.0 15 10985.0 16 9678.0 17 9197.0 18 8963.0 19 9369.0 20 10022.0 21 10919.0 22 11706.0 23 12665.0 24 14316.0 25 16420.0 26 20186.0 27 24977.0 28 30363.0 29 35975.0 30 41659.0 31 53466.0 32 73521.0 33 103988.0 34 159194.0 35 267339.0 36 505885.0 37 826492.0 38 258006.0 39 871.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.44296173027893 23.76486725398428 19.92608688976352 14.866084125973266 2 32.2087126392159 26.649471101104734 27.806443146247478 13.335373113431878 3 36.72743487032543 20.17331364579983 26.754225022660155 16.345026461214584 4 30.50513164222784 18.52485568398264 29.787534955247054 21.18247771854247 5 35.94212019116388 16.609965826003155 28.629740038614315 18.818173944218653 6 33.6330478121403 17.077334661610642 31.758000338886 17.531617187363057 7 36.26262213229609 19.371290212110967 24.93382713200766 19.43226052358529 8 29.548156934299907 30.497170846964345 21.473525268157122 18.481146950578626 9 32.81632447440941 25.77599698030224 22.241431990908783 19.166246554379565 10-11 27.410809197973972 30.108081030205202 26.280853735205582 16.200256036615244 12-13 28.958100477585884 25.218005916931858 26.214687842324192 19.609205763158073 14-15 26.122175530574914 25.373784688628376 28.15060916170441 20.3534306190923 16-17 27.809970567432522 25.87548883159552 28.557128841634533 17.757411759337423 18-19 27.24210251963385 25.99549072620188 28.955100912933563 17.807305841230708 20-21 25.640873082865124 28.36197952075164 27.877894694577044 18.119252701806193 22-23 28.59793675089409 25.981851590725608 27.128858089521923 18.291353568858383 24-25 24.738683375661882 29.59959376337965 27.26347627041162 18.398246590546847 26-27 23.377485479976148 30.88062338643524 27.411777151355622 18.33011398223299 28-29 24.54738071395594 29.862449777734906 27.027713390844006 18.562456117465153 30-31 26.509706023969432 27.43393332523522 27.571101162469063 18.48525948832628 32-33 22.691636339412938 30.019918444911887 29.082393624633575 18.206051591041607 34-35 27.318806689809172 27.601830321953102 27.316519519262368 17.762843468975355 36-37 23.584859740367158 29.25195350456028 29.571508093443228 17.59167866162933 38-39 23.223726710575196 31.461004796509766 27.349017914167913 17.966250578747125 40-41 27.714654552082727 26.840850412168653 26.84152753854679 18.60296749720182 42-43 27.335769282584753 26.754348392727444 27.383562065087506 18.52632025960029 44-45 23.965296785937614 28.753898191436818 28.701065241830154 18.579739780795414 46-47 24.625918900451328 29.249868254555444 27.515129904943763 18.60908294004946 48-49 21.284591056839147 29.30175480980409 29.248568772145596 20.165085361211162 50-51 20.121386722503583 27.780006698965686 30.541316694518013 21.557289884012718 52-53 25.794258901250704 27.154723934532083 27.01293790643147 20.03807925778574 54-55 26.737510432867236 26.968611823409915 27.41890178437942 18.87497595934343 56-57 27.155141255531113 26.88314019849141 26.840097307678164 19.121621238299316 58-59 25.594403316639852 27.276841199178453 26.71424137865411 20.414514105527587 60-61 21.85292497434538 27.29878480617109 26.651313229331723 24.196976990151807 62-63 24.697060747001064 27.324141021947213 26.896282373171253 21.082515857880463 64-65 22.092469571786985 28.75961185792515 29.099881003666844 20.048037566621023 66-67 20.813078753465614 28.724924723400346 27.773378740183123 22.688617782950914 68-69 20.69786336881426 27.239371958094683 32.64323562951009 19.41952904358097 70-71 20.1294597362696 31.29004801600375 29.572875430399844 19.00761681732681 72-73 19.897738758634716 34.57367554059922 26.403714240666204 19.12487146009986 74-75 20.176614590678785 34.45735079211512 26.17917293402251 19.186861683183583 76-77 20.478930167709155 33.845559481122706 26.06560123920606 19.60990911196208 78-79 20.192646815634774 33.669527441634486 26.628183157891655 19.50964258483909 80-81 19.568287754011067 33.615081004361144 26.810582945267857 20.006048296359936 82-83 19.55580142162723 33.09833522521298 26.798074070793692 20.547789282366104 84-85 19.669749809297222 32.52067448677669 27.28143009610981 20.52814560781628 86-87 19.986787990246533 32.051750088064814 27.854356806609665 20.107105115078983 88-89 20.451559327343823 32.25473697925435 27.48728924596279 19.80641444743904 90-91 20.905270676642207 31.644533225410548 26.907499877415912 20.542696220531333 92-93 20.712259446708224 31.936579758585136 26.663449655482246 20.68771113922439 94-95 20.344075455214288 31.88140849635781 27.082081069502518 20.692434978925384 96-97 20.67706771683332 32.251024320441566 26.681743268352836 20.39016469437227 98-99 21.043261364630247 31.65396397527211 26.57759595816913 20.725178701928513 100-101 21.18623846126539 31.63696596469 26.234241573516876 20.942554000527736 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 556.0 1 414.5 2 210.5 3 150.5 4 154.0 5 180.5 6 259.0 7 380.0 8 529.5 9 723.0 10 916.5 11 1002.5 12 994.5 13 933.0 14 829.0 15 763.5 16 645.0 17 501.0 18 467.0 19 499.0 20 570.0 21 696.0 22 884.0 23 1201.5 24 1664.0 25 2343.5 26 3262.5 27 4440.0 28 6033.0 29 7869.5 30 10124.5 31 13442.0 32 18998.0 33 28093.5 34 41214.5 35 59773.0 36 85795.5 37 117589.0 38 148109.0 39 168194.0 40 175850.5 41 169453.0 42 159517.0 43 156927.5 44 149922.0 45 142746.5 46 143883.0 47 137304.5 48 118455.5 49 99605.0 50 84911.0 51 75201.0 52 67635.5 53 58965.0 54 50361.5 55 42371.0 56 35148.0 57 28533.0 58 22942.0 59 18539.5 60 15040.5 61 12191.5 62 9767.0 63 7723.5 64 6104.0 65 4796.5 66 3681.0 67 2910.0 68 2271.5 69 1778.0 70 1380.0 71 1036.0 72 814.0 73 610.0 74 455.0 75 347.5 76 274.0 77 213.0 78 159.0 79 130.0 80 107.5 81 73.0 82 59.0 83 52.5 84 37.5 85 31.0 86 30.5 87 28.0 88 23.0 89 23.0 90 16.0 91 9.5 92 11.0 93 9.0 94 6.5 95 5.0 96 2.0 97 1.5 98 1.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2274575887890692 2 0.2196154814142055 3 0.20170629666509823 4 0.19163921929085465 5 0.19401008896232508 6 0.11766808554097775 7 0.09381348915418312 8 0.0894000240734459 9 0.08254273948519301 10-11 0.06446941760498394 12-13 0.09197150579404072 14-15 0.09483478685881654 16-17 0.08380112415697345 18-19 0.07822046169951233 20-21 0.07322339793041314 22-23 0.15246515733455887 24-25 0.6233381115475942 26-27 0.35355138039327255 28-29 0.17874533576985785 30-31 0.30865075630742517 32-33 0.3453809987562053 34-35 0.3273988641710528 36-37 0.3360434197424141 38-39 0.3434113531829837 40-41 0.34563632318236365 42-43 0.3656975280948056 44-45 0.3433566408059498 46-47 0.36535101637359074 48-49 0.3534419556392047 50-51 0.3590955679327111 52-53 0.3529495442458993 54-55 0.33773950343046605 56-57 0.3447426876908094 58-59 0.33578809531625575 60-61 0.31629225163316443 62-63 0.304474378193835 64-65 0.3039272544234957 66-67 0.31693056269856035 68-69 0.31512505425644055 70-71 0.30093631114564073 72-73 0.29263826729549425 74-75 0.29911256524450963 76-77 0.30080864893256154 78-79 0.30339836811216764 80-81 0.3140125692567506 82-83 0.3398915235938007 84-85 0.3266146534335664 86-87 0.3373018044141946 88-89 0.3384142894138846 90-91 0.3206874792548904 92-93 0.2999697258180412 94-95 0.3000791505721091 96-97 0.3084501442583008 98-99 0.3278000882693016 100-101 0.3349491722017355 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2741610.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.26792187657254 #Duplication Level Percentage of deduplicated Percentage of total 1 91.74836963833486 55.29483573666065 2 4.0154263475775895 4.840028028338743 3 1.3341802235564002 2.4122480844769565 4 0.66975182370651 1.614582023513437 5 0.3204449990014049 0.9656277082777517 6 0.2900164421051463 1.048721296542869 7 0.19641617894464294 0.8286316449551443 8 0.1503155384454266 0.7247364102291125 9 0.15540930679898826 0.8429576364948347 >10 0.9180473157599558 11.13288616269424 >50 0.09622138604733446 4.08451025873536 >100 0.09783588922240012 11.704997401918217 >500 0.005403574582491024 2.122629155502736 >1k 0.0021006536710123183 1.8319083520183645 >5k 0.0 0.0 >10k+ 6.0682245845583955E-5 0.550700099641605 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT 15058 0.5492393155846382 Illumina Single End PCR Primer 1 (97% over 34bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 2746 0.1001601248901193 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004668789506895583 0.0 0.0 2 0.005033538687121801 0.0 0.0 3 0.005981886555709966 0.0 0.0 4 0.0066384350801171575 0.0 0.0 5 0.008279806391135135 0.0 0.0 6 0.009957652620175736 0.0 0.0 7 0.012729746389894988 0.0 0.0 8 0.015210040815433267 0.0 0.0 9 0.017872709831084653 0.0 0.0 10-11 0.030037094991629005 0.0 0.0 12-13 0.04079719580830242 0.0 0.0 14-15 0.05150258424794191 0.0 0.0 16-17 0.05919879195071509 0.0 0.0 18-19 0.06899230743978904 0.0 0.0 20-21 0.07639671579838124 0.0 0.0 22-23 0.08330871276366807 0.0 0.0 24-25 0.09087725825336207 0.0 0.0 26-27 0.09851875357910134 0.0 0.0 28-29 0.1066891352161686 0.0 0.0 30-31 0.21064265158064058 0.0 0.0 32-33 0.22286174911821885 0.0 0.0 34-35 0.2328376391974059 0.0 1.8237459011310872E-5 36-37 0.24410838886639602 0.0 3.6474918022621744E-5 38-39 0.2566922355842005 0.0 3.6474918022621744E-5 40-41 0.27171990180952066 0.0 3.6474918022621744E-5 42-43 0.28928257483741304 0.0 3.6474918022621744E-5 44-45 0.30855956901236864 0.0 3.6474918022621744E-5 46-47 0.32984268367856845 0.0 3.6474918022621744E-5 48-49 0.35435382858977027 0.0 5.471237703393261E-5 50-51 0.38099875620529544 0.0 7.294983604524349E-5 52-53 0.41176534955737687 0.0 7.294983604524349E-5 54-55 0.44700012036722947 0.0 7.294983604524349E-5 56-57 0.48460576084855245 0.0 7.294983604524349E-5 58-59 0.5272084650989747 0.0 7.294983604524349E-5 60-61 0.5750817950036657 0.0 7.294983604524349E-5 62-63 0.6232104493345152 0.0 7.294983604524349E-5 64-65 0.6735640736647444 0.0 7.294983604524349E-5 66-67 0.7288053370100052 0.0 9.118729505655436E-5 68-69 0.7851043729779217 0.0 1.0942475406786524E-4 70-71 0.8454156499283267 0.0 1.0942475406786524E-4 72-73 0.9111434522050912 0.0 1.0942475406786524E-4 74-75 0.9831449403817465 0.0 1.0942475406786524E-4 76-77 1.0595234187211164 0.0 1.0942475406786524E-4 78-79 1.1426679943536828 0.0 1.0942475406786524E-4 80-81 1.2347853998198137 0.0 1.0942475406786524E-4 82-83 1.3328299794646212 0.0 1.0942475406786524E-4 84-85 1.4438049175484478 0.0 1.0942475406786524E-4 86-87 1.5587009093197062 0.0 1.0942475406786524E-4 88-89 1.6784298277289622 0.0 1.2766221307917611E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 2645 0.0 86.887535 9 AGAGCGG 2780 0.0 83.525 8 CGGGAGA 3890 0.0 83.1581 4 GAGAGCG 3540 0.0 82.05827 7 ATCGGGA 4780 0.0 81.36987 2 TCGGGAG 4150 0.0 79.32116 3 AAGCGGC 835 0.0 78.313034 9 ATTCGGA 435 0.0 76.32845 2 CGGAAAG 1370 0.0 75.47269 5 GATCGGG 5365 0.0 73.925385 1 AAGCGTC 345 0.0 70.04748 9 TCGGAGA 700 0.0 68.43733 4 TCGGGAA 1190 0.0 68.15957 3 GTTCGGA 440 0.0 67.91497 2 GATCCGG 780 0.0 66.29625 1 GAACCGG 265 0.0 64.44862 1 ATCCGGA 760 0.0 64.28376 2 ACCGGAA 350 0.0 63.695316 3 GGATCGG 1220 0.0 63.384754 1 TTCGGAG 465 0.0 63.243576 3 >>END_MODULE