FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n02_34139-1.342000000356e9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n02_34139-1.342000000356e9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2741610
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT150580.5492393155846382Illumina Single End PCR Primer 1 (97% over 34bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC27460.1001601248901193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC26450.086.8875359
AGAGCGG27800.083.5258
CGGGAGA38900.083.15814
GAGAGCG35400.082.058277
ATCGGGA47800.081.369872
TCGGGAG41500.079.321163
AAGCGGC8350.078.3130349
ATTCGGA4350.076.328452
CGGAAAG13700.075.472695
GATCGGG53650.073.9253851
AAGCGTC3450.070.047489
TCGGAGA7000.068.437334
TCGGGAA11900.068.159573
GTTCGGA4400.067.914972
GATCCGG7800.066.296251
GAACCGG2650.064.448621
ATCCGGA7600.064.283762
ACCGGAA3500.063.6953163
GGATCGG12200.063.3847541
TTCGGAG4650.063.2435763
Produced by FastQC (version 0.11.2)