##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n02_34098-1.34200000035704.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2315508 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.144983303879755 33.0 33.0 33.0 27.0 33.0 2 30.769280434358247 33.0 33.0 33.0 27.0 33.0 3 30.59747882538087 33.0 33.0 33.0 27.0 33.0 4 34.04771782261171 37.0 37.0 37.0 27.0 37.0 5 34.13706970565423 37.0 37.0 37.0 27.0 37.0 6 34.58363909777034 37.0 37.0 37.0 27.0 37.0 7 33.991325013776674 37.0 37.0 37.0 27.0 37.0 8 34.32168275816797 37.0 37.0 37.0 27.0 37.0 9 33.81267264030183 37.0 37.0 37.0 27.0 37.0 10-11 33.799219221008954 37.0 37.0 37.0 24.5 37.0 12-13 33.78857533638407 37.0 37.0 37.0 24.5 37.0 14-15 35.261519070545205 40.0 37.0 40.0 22.0 40.0 16-17 35.15068745173845 38.5 37.0 40.0 24.5 40.0 18-19 35.17182385031708 38.5 37.0 40.0 22.0 40.0 20-21 35.217235699466386 40.0 37.0 40.0 22.0 40.0 22-23 35.81865642442177 37.0 37.0 40.0 27.0 40.0 24-25 35.118778471074165 37.0 37.0 40.0 22.0 40.0 26-27 35.02959566540042 37.0 37.0 40.0 21.0 40.0 28-29 34.80461393352992 37.0 37.0 40.0 18.5 40.0 30-31 34.77325817919869 37.0 37.0 40.0 18.5 40.0 32-33 34.57429622355008 37.0 37.0 40.0 15.0 40.0 34-35 34.659966193163655 37.0 37.0 40.0 18.5 40.0 36-37 34.510581479312535 37.0 37.0 40.0 15.0 40.0 38-39 34.61447056110366 37.0 37.0 40.0 18.5 40.0 40-41 34.22087226647457 37.0 33.0 40.0 15.0 40.0 42-43 34.11447682322842 37.0 33.0 40.0 15.0 40.0 44-45 33.996312472252306 37.0 33.0 40.0 15.0 40.0 46-47 33.93719909410808 37.0 33.0 40.0 15.0 40.0 48-49 33.811271219965555 37.0 33.0 40.0 15.0 40.0 50-51 32.81687128699188 37.0 33.0 38.5 15.0 38.5 52-53 33.32514182632926 37.0 33.0 38.5 18.5 40.0 54-55 33.96163692805207 37.0 33.0 40.0 18.5 40.0 56-57 34.18932260221084 37.0 33.0 40.0 18.5 40.0 58-59 33.69887342216049 37.0 33.0 40.0 15.0 40.0 60-61 33.505629218296804 37.0 33.0 40.0 15.0 40.0 62-63 33.09401306322414 37.0 33.0 40.0 15.0 40.0 64-65 32.868084238966134 37.0 33.0 37.0 15.0 40.0 66-67 32.737216628057425 37.0 33.0 37.0 15.0 40.0 68-69 32.81406110451789 37.0 33.0 37.0 18.5 40.0 70-71 32.367988579611904 37.0 33.0 37.0 15.0 38.5 72-73 32.53545787792571 37.0 33.0 37.0 22.0 37.0 74-75 32.54378715167471 37.0 33.0 37.0 22.0 37.0 76-77 32.2034074164287 37.0 33.0 37.0 22.0 37.0 78-79 31.728783489411395 35.0 33.0 37.0 22.0 37.0 80-81 31.315068442864373 33.0 33.0 37.0 15.0 37.0 82-83 30.979444683412886 33.0 33.0 37.0 15.0 37.0 84-85 30.77765591826934 33.0 33.0 37.0 15.0 37.0 86-87 30.61143256684926 33.0 27.0 37.0 15.0 37.0 88-89 30.47423956211769 33.0 27.0 37.0 15.0 37.0 90-91 30.323463576891122 33.0 27.0 37.0 15.0 37.0 92-93 30.157105265885498 33.0 27.0 37.0 15.0 37.0 94-95 30.027312365148383 33.0 27.0 37.0 15.0 37.0 96-97 29.81716042440795 33.0 27.0 37.0 15.0 37.0 98-99 29.683320679522595 33.0 27.0 37.0 15.0 37.0 100-101 28.47075652513401 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.060641354965273564 2306 2 -0.2136838764126061 2306 3 -0.15983116698196298 2306 4 -0.04263212169843911 2306 5 -0.1258092192371194 2306 6 -0.2901107920850805 2306 7 0.30206732592068164 2306 8 -0.3339675644117648 2306 9 -0.06603333457797333 2306 10-11 -0.016688084958950355 2306 12-13 -0.06521891842598393 2306 14-15 0.2957425364441377 2306 16-17 0.21961996959125685 2306 18-19 0.30572369914978736 2306 20-21 0.3955868715166915 2306 22-23 0.2875950930413538 2306 24-25 0.39024133889333257 2306 26-27 0.16173156987252923 2306 28-29 0.3007810044260921 2306 30-31 0.3119513447731208 2306 32-33 0.310383839623924 2306 34-35 0.21740270248794502 2306 36-37 0.20302337218956268 2306 38-39 0.040026415139060134 2306 40-41 0.3218249022992197 2306 42-43 0.05459605340367801 2306 44-45 0.39194444878412327 2306 46-47 0.3891953639330339 2306 48-49 0.3696759034275132 2306 50-51 0.14315771889929607 2306 52-53 0.17067508475855675 2306 54-55 0.3187913110313474 2306 56-57 0.4467497275036507 2306 58-59 0.5469924179712251 2306 60-61 0.33570412422928797 2306 62-63 0.43384712040104745 2306 64-65 0.4146769006232134 2306 66-67 0.38433072677047164 2306 68-69 0.22480487454857467 2306 70-71 0.5161162976979199 2306 72-73 0.2510394009836574 2306 74-75 0.21947736038156052 2306 76-77 0.41879604616021027 2306 78-79 0.5083138698736427 2306 80-81 0.5903320405319761 2306 82-83 0.5425916222047604 2306 84-85 0.5498699776627411 2306 86-87 0.45860549174783216 2306 88-89 0.28281431065367 2306 90-91 0.2770258069833069 2306 92-93 0.24033718023147088 2306 94-95 0.35435219602694445 2306 96-97 0.36025198701847216 2306 98-99 0.37488238203558666 2306 100-101 0.29165862948461907 2307 1 -0.08505049870800718 2307 2 -0.14713589313961606 2307 3 -0.14852907877771315 2307 4 -0.1468920316504807 2307 5 -0.1513847112276565 2307 6 -0.06368224928557709 2307 7 0.3432929935982685 2307 8 -0.11841157835984717 2307 9 -0.05109125118340785 2307 10-11 -0.00795349932653977 2307 12-13 -0.04833492531986394 2307 14-15 0.3540646249155941 2307 16-17 0.26336470899993714 2307 18-19 0.32378539670085615 2307 20-21 0.2024630488871253 2307 22-23 0.3475558139594668 2307 24-25 0.34293776770334006 2307 26-27 0.2940623291345048 2307 28-29 0.321074862727464 2307 30-31 0.4227330519435526 2307 32-33 0.3964922044651402 2307 34-35 0.16452357250624772 2307 36-37 0.14361442885031295 2307 38-39 0.189803299265769 2307 40-41 0.4198465859556535 2307 42-43 0.2002245855149951 2307 44-45 0.4743683956638378 2307 46-47 0.2422591991212073 2307 48-49 0.2752367659640882 2307 50-51 0.38685502948460737 2307 52-53 0.2582497350544841 2307 54-55 0.24328863230483933 2307 56-57 0.3445740137332578 2307 58-59 0.38579954966303376 2307 60-61 0.37604567855866833 2307 62-63 0.372623857565209 2307 64-65 0.5238664021529544 2307 66-67 0.39896018073779516 2307 68-69 0.35544389444196867 2307 70-71 0.3458568419937649 2307 72-73 0.10087774217726064 2307 74-75 0.25006193732981785 2307 76-77 0.5516535325661778 2307 78-79 0.6084167436595429 2307 80-81 0.40777248729813564 2307 82-83 0.4269310923379628 2307 84-85 0.5038172581875315 2307 86-87 0.42550150906269124 2307 88-89 0.4336342948885985 2307 90-91 0.5051764836790547 2307 92-93 0.486592975232071 2307 94-95 0.476846484527794 2307 96-97 0.44667331529273113 2307 98-99 0.38828236065921473 2307 100-101 0.3326528311439638 2201 1 0.10986496008343494 2201 2 -0.0017328590696905621 2201 3 0.1619930129685052 2201 4 0.1710788586571539 2201 5 0.2318904912247035 2201 6 0.13245275113683164 2201 7 0.5685829439959349 2201 8 0.16555093627344064 2201 9 0.36594371182604135 2201 10-11 0.4147854306629526 2201 12-13 0.37517670286778326 2201 14-15 0.5948580209256846 2201 16-17 0.6221157197531468 2201 18-19 0.7014522765811364 2201 20-21 0.4718770534008172 2201 22-23 0.43145738168294656 2201 24-25 0.5554901156679435 2201 26-27 0.30857704690807 2201 28-29 0.6014460354425566 2201 30-31 0.5684697854305583 2201 32-33 0.5441029045701384 2201 34-35 0.4161824146356494 2201 36-37 0.4711702767364727 2201 38-39 0.46481385937390485 2201 40-41 0.6484056441146961 2201 42-43 0.5393742252644529 2201 44-45 0.7649723131622395 2201 46-47 0.5436630900684776 2201 48-49 0.48786514670727854 2201 50-51 0.35923883812338175 2201 52-53 0.34456051601973314 2201 54-55 0.6536459863641468 2201 56-57 0.7423551841553078 2201 58-59 0.8592680322660087 2201 60-61 0.7282129144654519 2201 62-63 0.7565803378509983 2201 64-65 0.6318030777102308 2201 66-67 0.5316283278310507 2201 68-69 0.5519862133210225 2201 70-71 0.7595777413529134 2201 72-73 0.5064275315785238 2201 74-75 0.6216633928144333 2201 76-77 0.6020614544575764 2201 78-79 0.6672715451898448 2201 80-81 0.7352492852319727 2201 82-83 0.8381667456767694 2201 84-85 0.772747006313864 2201 86-87 0.5316040446955128 2201 88-89 0.7174178911183375 2201 90-91 0.7816208047546205 2201 92-93 0.6570240690179006 2201 94-95 0.7768854060692085 2201 96-97 0.8569057583586606 2201 98-99 0.5790645931264216 2201 100-101 0.6224448271854293 1101 1 -0.02962093245481512 1101 2 -0.1290684545021321 1101 3 -0.09893453525986118 1101 4 -0.0862940406071715 1101 5 -0.005268045133668409 1101 6 0.09738419289106304 1101 7 0.3285442277207551 1101 8 0.006413468313461124 1101 9 0.1620093455477445 1101 10-11 0.1758961038071547 1101 12-13 0.08237611248837595 1101 14-15 0.30595560980832914 1101 16-17 0.3908601562046883 1101 18-19 0.458847074191155 1101 20-21 0.36804878460738877 1101 22-23 0.46409878203835575 1101 24-25 0.5147857444296804 1101 26-27 0.29322822268499493 1101 28-29 0.34850645626307397 1101 30-31 0.47176121627941825 1101 32-33 0.46612563286312536 1101 34-35 0.4208396453051577 1101 36-37 0.3495605512431865 1101 38-39 0.36426240428357914 1101 40-41 0.4435718032871918 1101 42-43 0.4357942289652641 1101 44-45 0.471403638307784 1101 46-47 0.42986614685855074 1101 48-49 0.4688070164537308 1101 50-51 0.38708696433668877 1101 52-53 0.3674609847538264 1101 54-55 0.6365504953430161 1101 56-57 0.4914231358168948 1101 58-59 0.5775395191965345 1101 60-61 0.3703014253888952 1101 62-63 0.5254775152637308 1101 64-65 0.40649619976831985 1101 66-67 0.4992505288118778 1101 68-69 0.3956035572487835 1101 70-71 0.5262563426179838 1101 72-73 0.500838800029257 1101 74-75 0.5292058400202606 1101 76-77 0.456929620941942 1101 78-79 0.6658180424947098 1101 80-81 0.6583297036217672 1101 82-83 0.6979349407384703 1101 84-85 0.5864232896390433 1101 86-87 0.5833323168831903 1101 88-89 0.5476090855745497 1101 90-91 0.5289672727156933 1101 92-93 0.4270057620009027 1101 94-95 0.5214773358718716 1101 96-97 0.5725790065731253 1101 98-99 0.5302296398629629 1101 100-101 0.5265059848852722 2304 1 0.13221011042513098 2304 2 0.10114923230742434 2304 3 0.17061805767935212 2304 4 0.19762169230541105 2304 5 0.1817384875203487 2304 6 0.3560456962263814 2304 7 0.36510474313400465 2304 8 0.2448235942021597 2304 9 0.23066120513193056 2304 10-11 0.27277193338722583 2304 12-13 0.29955123830958996 2304 14-15 0.4420144901833183 2304 16-17 0.4712891156544359 2304 18-19 0.43853183001527896 2304 20-21 0.4172087933862514 2304 22-23 0.33011363311513975 2304 24-25 0.5871964922378297 2304 26-27 0.5205204133463042 2304 28-29 0.5073345130297255 2304 30-31 0.45697213146590343 2304 32-33 0.41642031970118865 2304 34-35 0.3760810665411043 2304 36-37 0.40870389218054015 2304 38-39 0.35685698828494594 2304 40-41 0.5270693653529079 2304 42-43 0.6438610343139715 2304 44-45 0.4770332842686287 2304 46-47 0.4730121844466808 2304 48-49 0.44635267429104175 2304 50-51 0.37745821126020473 2304 52-53 0.5054689877174425 2304 54-55 0.7668003779832162 2304 56-57 0.7323935551475955 2304 58-59 0.6582308196731219 2304 60-61 0.6024387386068355 2304 62-63 0.6592605653587427 2304 64-65 0.6285675665263355 2304 66-67 0.5165230420214115 2304 68-69 0.4374760097661792 2304 70-71 0.4999090045982584 2304 72-73 0.5102464567377112 2304 74-75 0.6337374281795931 2304 76-77 0.6633242678351721 2304 78-79 0.7105782460483532 2304 80-81 0.6843117139514305 2304 82-83 0.5428486494131981 2304 84-85 0.5215239441042883 2304 86-87 0.4155809156896453 2304 88-89 0.34753224552533624 2304 90-91 0.637567334007759 2304 92-93 0.5918260427745423 2304 94-95 0.5999919182334565 2304 96-97 0.6056218525245747 2304 98-99 0.36960844030830486 2304 100-101 0.43328805660698677 2202 1 -0.10132971561835546 2202 2 -0.1659699584884784 2202 3 -0.15066686290606768 2202 4 -0.11333575433171461 2202 5 -0.13303704515328008 2202 6 0.11595620710193799 2202 7 0.12936589285430244 2202 8 0.18256992001354888 2202 9 -0.10680650856639318 2202 10-11 0.21445210757848088 2202 12-13 0.16438500887782936 2202 14-15 0.1231694393457019 2202 16-17 0.2222184939508196 2202 18-19 0.3393467462808957 2202 20-21 0.27817327091719335 2202 22-23 0.27414115923703974 2202 24-25 0.24420728299130445 2202 26-27 0.28869308749555955 2202 28-29 0.16763118222720408 2202 30-31 0.2686544160760178 2202 32-33 0.0903737234017612 2202 34-35 0.21435221232824375 2202 36-37 0.009096425486376347 2202 38-39 0.10611678821172177 2202 40-41 0.2444366396836628 2202 42-43 0.10228486211669008 2202 44-45 0.1302792672807911 2202 46-47 0.12156772125830884 2202 48-49 0.10578883573140985 2202 50-51 0.10661391253260888 2202 52-53 0.1583421377350831 2202 54-55 0.2125402892259416 2202 56-57 0.293883036769337 2202 58-59 0.21155986159128304 2202 60-61 0.12266711492604543 2202 62-63 0.3672866470980054 2202 64-65 0.2450714332877837 2202 66-67 0.40361195049298004 2202 68-69 0.3257434847593217 2202 70-71 0.3138625786052671 2202 72-73 0.38883903059488745 2202 74-75 0.5134650713756557 2202 76-77 0.41354250171607276 2202 78-79 0.474535145633606 2202 80-81 0.4610847477234259 2202 82-83 0.41999799135145466 2202 84-85 0.32961909200506767 2202 86-87 0.26395526026880134 2202 88-89 0.2792534060301719 2202 90-91 0.18613757700972045 2202 92-93 0.29050552354245696 2202 94-95 0.2940944137059489 2202 96-97 0.35517462553901424 2202 98-99 0.3241278781405903 2202 100-101 0.46370052727227673 1102 1 0.011165292501061685 1102 2 -0.059380040551289426 1102 3 0.002143049459281343 1102 4 -0.011557108881817157 1102 5 -0.11581010499335065 1102 6 0.1159877737950552 1102 7 -0.026529716727139885 1102 8 0.09185169300241114 1102 9 -0.07852169225171224 1102 10-11 0.08051950140899322 1102 12-13 0.072370428879033 1102 14-15 0.015001292681333211 1102 16-17 0.20958810920791393 1102 18-19 0.16860366844357344 1102 20-21 -0.021666988904229356 1102 22-23 0.19647533611433943 1102 24-25 0.12525635648766809 1102 26-27 0.09241054228792223 1102 28-29 0.10242837681679617 1102 30-31 0.19204088667609653 1102 32-33 0.21633330320458555 1102 34-35 0.2119365128770596 1102 36-37 0.08620326391258004 1102 38-39 0.11870988612248823 1102 40-41 0.0658410821131028 1102 42-43 0.26795862563977835 1102 44-45 0.3124235355425995 1102 46-47 0.15920634194211658 1102 48-49 0.11633740765632439 1102 50-51 -0.059482505707009636 1102 52-53 0.1151656809798638 1102 54-55 0.30335660602843006 1102 56-57 0.3219803596383528 1102 58-59 0.16639343202790968 1102 60-61 0.21506399088271166 1102 62-63 0.2584039067700701 1102 64-65 0.21413766296408454 1102 66-67 0.24639848443830203 1102 68-69 0.23637701134155265 1102 70-71 0.3978389862096634 1102 72-73 0.39317616711448267 1102 74-75 0.3157726178279532 1102 76-77 0.4096087409259255 1102 78-79 0.2846906168936165 1102 80-81 0.40247535589072214 1102 82-83 0.3391099291756632 1102 84-85 0.3908680102102551 1102 86-87 0.494165115220909 1102 88-89 0.3047194649497236 1102 90-91 0.22416347229092182 1102 92-93 0.30082560769205813 1102 94-95 0.2832057053663064 1102 96-97 0.4867105993873828 1102 98-99 0.4048731486978703 1102 100-101 0.27425566676422264 2305 1 0.18663019863537755 2305 2 -0.0412936367524388 2305 3 -0.010553067077058387 2305 4 -0.1025608815915362 2305 5 -0.01840422373297912 2305 6 0.2245948390514343 2305 7 -0.18638372200048536 2305 8 0.19002174698390206 2305 9 -0.13217062682237213 2305 10-11 -0.007471312008448194 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1213 66-67 -0.26914712753035985 1213 68-69 -0.19524523480649947 1213 70-71 -0.3484496211290775 1213 72-73 -0.08923832537840326 1213 74-75 -0.11835005701722423 1213 76-77 -0.17583607802332324 1213 78-79 -0.3307044591333579 1213 80-81 -0.6039066793102279 1213 82-83 -0.5535112796659014 1213 84-85 -0.46173502468428396 1213 86-87 -0.509452385741902 1213 88-89 -0.3714600143730209 1213 90-91 -0.450099028095611 1213 92-93 -0.28452166509143595 1213 94-95 -0.3026580420369136 1213 96-97 -0.42647303197244213 1213 98-99 -0.35072725216908296 1213 100-101 -0.19359879548091286 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 806.0 3 240.0 4 327.0 5 320.0 6 983.0 7 2753.0 8 12584.0 9 37120.0 10 46592.0 11 33405.0 12 20953.0 13 14355.0 14 11122.0 15 9017.0 16 8199.0 17 8062.0 18 8333.0 19 8617.0 20 9358.0 21 9928.0 22 10828.0 23 11964.0 24 13643.0 25 15459.0 26 18578.0 27 22679.0 28 27804.0 29 33648.0 30 40181.0 31 51930.0 32 72246.0 33 103063.0 34 156208.0 35 260479.0 36 474113.0 37 634612.0 38 124921.0 39 78.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.40808235228429 23.464150933596265 22.174918530259475 13.952848183859967 2 31.99149800672317 25.712470412466125 29.823931180770362 12.472100400040336 3 36.30116654132053 19.65045302029396 28.420673199667323 15.627707238718196 4 30.066005958059932 17.440197650092703 32.19818662097523 20.29560977087214 5 34.71805569437972 15.7771463261501 31.961678472850537 17.543119506619643 6 31.480527049919623 16.451069224376706 35.98005532692436 16.088348398779313 7 35.81634889421932 18.89654927954912 27.7834717981726 17.50363002805896 8 29.426565748693502 30.200957020527962 23.098516056245487 17.273961174533053 9 31.84626516272701 26.58785045436313 23.409037469397916 18.15684691351194 10-11 26.683357832773012 29.15948303337275 27.541353203124373 16.61580593072986 12-13 28.311075675029617 24.35254885831386 27.391476219942216 19.944899246714304 14-15 25.587290897034325 24.546844510995818 29.296742277595882 20.56912231437397 16-17 26.942740352592487 25.44832635919965 30.02490978146562 17.584023506742238 18-19 26.443548950017536 25.60138463310529 30.018003774503967 17.937062642373206 20-21 25.123044448996843 28.015280504175387 28.790391382480234 18.071283664347536 22-23 27.897100590807415 25.46646190936829 28.338059749425337 18.29837775039895 24-25 24.211715626629584 28.74404658439075 28.503172257952375 18.54106553102729 26-27 22.740297279721307 29.881221164228922 28.907574060434122 18.470907495615652 28-29 23.565013307535267 29.072773170070583 28.30170842230803 19.060505100086118 30-31 25.71774288928852 27.060452474024615 28.196359742020228 19.025444894666634 32-33 22.65108619101793 26.87311338642397 29.824952597648846 20.650847824909256 34-35 20.369286686609176 26.805284945557467 33.97765534759179 18.84777302024157 36-37 21.67123710339472 27.58361125748835 30.138772846405594 20.60637879271134 38-39 20.302180597600326 27.154691762788772 28.12242810615434 24.42069953345656 40-41 22.364094226240667 26.40890905044572 29.42974041995587 21.797256303357752 42-43 24.18333165355901 26.54021759037999 30.582599922188518 18.693850833872485 44-45 21.78438697154036 28.729334923272713 30.925982225644987 18.56029587954194 46-47 23.381110574304415 29.32915274346875 28.879535592126327 18.41020109010051 48-49 20.64594846455584 28.719502416401188 30.613007390070003 20.021541728972974 50-51 19.80246344195272 27.510819170738305 30.982468262031993 21.70424912527698 52-53 24.75224801551354 26.85387053961908 28.11848491599578 20.2753965288716 54-55 26.11176138434384 26.76441566196924 28.300940770340492 18.82288218334643 56-57 26.496378804249137 26.72564246916534 27.381990970738762 19.395987755846768 58-59 24.625177888290487 26.730889900798573 27.62147971856816 21.022452492342786 60-61 20.92699270134977 27.04425895260946 27.439779420111275 24.58896892592949 62-63 23.641645203122636 27.15299508766245 27.825330541466137 21.38002916774878 64-65 21.37243434789766 28.57770384425441 29.906492865126193 20.143368942721736 66-67 20.659703455140793 28.374722307369858 28.522074059311137 22.443500178178205 68-69 20.784010529900428 26.77824213398588 33.038559088148055 19.399188247965636 70-71 20.28408233141668 31.34468337094514 29.648545636997632 18.722688660640546 72-73 20.071289156407317 34.37917185373465 26.831717220545904 18.71782176931214 74-75 20.77646695967307 33.72592898710061 26.512205856489242 18.985398196737076 76-77 20.833316186196676 33.046200017630866 26.66932790227562 19.45115589389684 78-79 20.670517997582287 32.81265060619365 27.214199398322503 19.30263199790156 80-81 19.794171395594883 32.88127985636882 27.431033033610703 19.89351571442559 82-83 19.157897656370363 32.43606042096017 27.834326214333586 20.571715708335887 84-85 19.386723123693578 31.80747125440673 28.1213493876504 20.68445623424929 86-87 19.356697256691643 31.697825474131662 28.9018207756633 20.04365649351339 88-89 19.73906708824335 31.909948655010904 28.677268488183028 19.673715768562722 90-91 20.507230065947667 31.148480708017207 27.843515059685657 20.500774166349466 92-93 19.93611336369118 31.849207958192117 27.5631978666334 20.651480811483307 94-95 19.754645369878418 31.844378999022936 27.817225767282828 20.58374986381581 96-97 20.256412644699726 32.383603921809026 27.245971715199012 20.11401171829224 98-99 20.683056823906014 31.493020912887342 27.14682352686285 20.677098736343794 100-101 20.681268317246584 31.574242078217434 26.735433848169933 21.00905575636605 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 476.0 1 351.0 2 181.0 3 137.5 4 138.5 5 180.0 6 270.5 7 393.5 8 525.5 9 653.0 10 767.5 11 857.0 12 885.5 13 798.0 14 668.5 15 601.5 16 552.5 17 461.5 18 418.5 19 430.0 20 456.0 21 519.0 22 651.5 23 800.5 24 973.0 25 1252.0 26 1716.0 27 2400.5 28 3203.5 29 4072.0 30 5106.0 31 6671.0 32 10892.5 33 19322.5 34 33250.0 35 53413.0 36 80959.5 37 114839.5 38 144166.0 39 159925.0 40 162972.0 41 150921.0 42 141159.5 43 144528.5 44 141382.5 45 132169.5 46 126958.0 47 114592.5 48 94238.5 49 79429.0 50 69059.5 51 58800.5 52 48553.5 53 41230.5 54 34456.0 55 26305.0 56 20334.5 57 16494.0 58 13214.5 59 10460.5 60 8157.5 61 6187.5 62 4698.5 63 3560.5 64 2778.0 65 2119.5 66 1590.0 67 1252.5 68 1005.5 69 740.0 70 511.0 71 369.5 72 265.0 73 205.0 74 153.0 75 119.0 76 95.0 77 64.0 78 47.5 79 42.5 80 29.0 81 21.5 82 24.5 83 21.0 84 16.5 85 13.5 86 9.5 87 9.0 88 8.5 89 6.0 90 5.0 91 4.5 92 5.5 93 6.0 94 2.5 95 1.5 96 3.0 97 1.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22202471336743385 2 0.21554665326140096 3 0.1975376461666295 4 0.18799330427707442 5 0.1913618955322115 6 0.1170369525823275 7 0.09350000086374134 8 0.08974272600224227 9 0.08378291070469202 10-11 0.06519087820037762 12-13 0.09110311862450918 14-15 0.09388868447010332 16-17 0.08460346498478952 18-19 0.0788811785577938 20-21 0.07384988520877492 22-23 0.15126270347586793 24-25 0.6179205599807904 26-27 0.35208256676288746 28-29 0.18240057905219934 30-31 0.3137540444688595 32-33 0.3512404189491032 34-35 0.33286432178165654 36-37 0.3422143218680307 38-39 0.3503766776016321 40-41 0.3518234443586461 42-43 0.3735897263149166 44-45 0.34905947204673876 46-47 0.37384884871915797 48-49 0.3609575091081525 50-51 0.36674457613620853 52-53 0.3611302573776467 54-55 0.34251663133964555 56-57 0.35072217414062057 58-59 0.3403140909035944 60-61 0.3200161692380246 62-63 0.30820450631135804 64-65 0.3078374162386828 66-67 0.3206855687823147 68-69 0.3201889175075189 70-71 0.3047927279888474 72-73 0.2972133976647889 74-75 0.3034323353665805 76-77 0.3047495409214738 78-79 0.3078590097723696 80-81 0.31846143481257677 82-83 0.3453885713199868 84-85 0.33150392915938964 86-87 0.3432292179513092 88-89 0.3446327976409496 90-91 0.32569526859764686 92-93 0.3042097025793044 94-95 0.3046199797193532 96-97 0.3125232130487133 98-99 0.33342575365751276 100-101 0.34182563826166873 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2315508.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.50494130453592 #Duplication Level Percentage of deduplicated Percentage of total 1 87.11115802618247 37.02654658873039 2 5.669499785058896 4.819635111800149 3 1.9910920195090327 2.53893748263484 4 1.0569872204818471 1.7970871906490191 5 0.6737548268972463 1.4318954685457608 6 0.49014015392665744 1.2500027084189267 7 0.3623981607454493 1.0782598785949966 8 0.25354816936651164 0.8621640045436888 9 0.23440597080037498 0.8967070827272429 >10 1.6624520643357608 14.218368879767556 >50 0.24327049646732385 7.179945855191101 >100 0.23339148368245383 19.77233485257285 >500 0.013477356598853973 3.6974062805099353 >1k 0.00432198691230455 3.048010130113685 >5k 1.0227903470193592E-4 0.382698485199754 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT 8803 0.3801757540893834 Illumina Single End PCR Primer 1 (96% over 32bp) GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTCTTCGCCTGTGTAGATCT 3575 0.1543937658604505 Illumina Single End PCR Primer 1 (96% over 32bp) TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 3429 0.14808845402391183 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 3179 0.13729168718052367 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3140 0.13560739155295512 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 2791 0.12053510503958528 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 2780 0.12006004729847618 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 2594 0.1120272527669954 No Hit TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT 2574 0.11116351141952435 No Hit GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT 2369 0.10231016260794608 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.00483695154583789 0.0 0.0 2 0.005268822219573415 0.0 0.0 3 0.006046189432297362 0.0 0.0 4 0.006607621308153545 0.0 0.0 5 0.00768729799249236 0.0 0.0 6 0.009285219485313806 0.0 0.0 7 0.011142263382376567 0.0 0.0 8 0.012481062470956697 0.0 0.0 9 0.014467667570140116 0.0 0.0 10-11 0.02161512722046307 0.0 0.0 12-13 0.02820115499492984 0.0 0.0 14-15 0.036385104262218054 0.0 0.0 16-17 0.04230173249239476 0.0 0.0 18-19 0.04787286418358304 0.0 0.0 20-21 0.05458845316017047 0.0 0.0 22-23 0.06121766800201079 0.0 0.0 24-25 0.06817078584915276 0.0 0.0 26-27 0.07546940023528315 0.0 0.0 28-29 0.08341582063201682 0.0 0.0 30-31 0.1400772530261178 0.0 0.0 32-33 0.15098198753793984 0.0 0.0 34-35 0.1612173225054718 0.0 0.0 36-37 0.17091281913083434 0.0 0.0 38-39 0.18423602941557532 0.0 0.0 40-41 0.19853094871622123 0.0 0.0 42-43 0.21530912439084643 0.0 0.0 44-45 0.23314538321612363 0.0 0.0 46-47 0.25400473675754953 0.0 0.0 48-49 0.27605173465174815 0.0 0.0 50-51 0.3017048526716384 0.0 0.0 52-53 0.33115843262040123 0.0 0.0 54-55 0.36220993406198554 0.0 0.0 56-57 0.3963924978881524 0.0 0.0 58-59 0.4317411125334052 0.0 0.0 60-61 0.4713004662475794 0.0 0.0 62-63 0.5152649008338559 0.0 0.0 64-65 0.5603090121044713 0.0 0.0 66-67 0.608592153428103 0.0 0.0 68-69 0.6607405372816677 0.0 0.0 70-71 0.7168189442662258 0.0 0.0 72-73 0.772400699975988 0.0 4.3187067373552583E-5 74-75 0.8311351116040195 0.0 4.3187067373552583E-5 76-77 0.8938427334304179 0.0 4.3187067373552583E-5 78-79 0.9647127109904177 0.0 4.3187067373552583E-5 80-81 1.0407867301689304 0.0 4.3187067373552583E-5 82-83 1.1233172159197897 0.0 4.3187067373552583E-5 84-85 1.2135349996631408 0.0 4.3187067373552583E-5 86-87 1.3071429681953162 0.0 4.3187067373552583E-5 88-89 1.4064300360871136 0.0 4.3187067373552583E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGAGA 2480 0.0 84.91082 4 GAGCGGC 1875 0.0 84.891174 9 AGAGCGG 2005 0.0 82.22226 8 TCGGGAG 2605 0.0 77.92844 3 CGGAAAG 1210 0.0 75.649086 5 ATTCGGA 365 0.0 75.3743 2 TCGGAGA 435 0.0 75.23009 4 AAGCGGC 720 0.0 75.00616 9 GAGAGCG 2490 0.0 74.57648 7 AAGCGTC 400 0.0 73.42707 9 GGATCGG 685 0.0 72.722946 1 GAACCGG 310 0.0 71.92981 1 GATCGGG 3715 0.0 69.7279 1 TCGGGAA 925 0.0 69.22325 3 GATCCGG 530 0.0 68.03156 1 ATCGGGA 3655 0.0 67.744064 2 ACCGGAA 470 0.0 64.58655 3 AATCGGA 450 0.0 64.29919 2 GAATCGG 505 0.0 62.004803 1 AACCGGA 340 0.0 61.384953 2 >>END_MODULE