FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n02_34098-1.34200000035704.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n02_34098-1.34200000035704.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2315508
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT88030.3801757540893834Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTCTTCGCCTGTGTAGATCT35750.1543937658604505Illumina Single End PCR Primer 1 (96% over 32bp)
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA34290.14808845402391183No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG31790.13729168718052367No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC31400.13560739155295512No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC27910.12053510503958528No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG27800.12006004729847618No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC25940.1120272527669954No Hit
TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT25740.11116351141952435No Hit
GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT23690.10231016260794608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAGA24800.084.910824
GAGCGGC18750.084.8911749
AGAGCGG20050.082.222268
TCGGGAG26050.077.928443
CGGAAAG12100.075.6490865
ATTCGGA3650.075.37432
TCGGAGA4350.075.230094
AAGCGGC7200.075.006169
GAGAGCG24900.074.576487
AAGCGTC4000.073.427079
GGATCGG6850.072.7229461
GAACCGG3100.071.929811
GATCGGG37150.069.72791
TCGGGAA9250.069.223253
GATCCGG5300.068.031561
ATCGGGA36550.067.7440642
ACCGGAA4700.064.586553
AATCGGA4500.064.299192
GAATCGG5050.062.0048031
AACCGGA3400.061.3849532
Produced by FastQC (version 0.11.2)