##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n02_34042-1.34200000035847.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5284939 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.062468649117804 33.0 33.0 33.0 27.0 33.0 2 30.55546790606287 33.0 33.0 33.0 27.0 33.0 3 30.402768508775598 33.0 33.0 33.0 22.0 33.0 4 33.685339603730526 37.0 37.0 37.0 22.0 37.0 5 33.78671958181542 37.0 37.0 37.0 22.0 37.0 6 34.45259727690329 37.0 37.0 37.0 27.0 37.0 7 33.66655376722418 37.0 37.0 37.0 22.0 37.0 8 34.17613221268968 37.0 37.0 37.0 27.0 37.0 9 33.468882611511695 37.0 37.0 37.0 15.0 37.0 10-11 33.50829035112798 37.0 37.0 37.0 18.5 37.0 12-13 33.498935181654886 37.0 37.0 37.0 18.5 37.0 14-15 34.99012438932597 40.0 37.0 40.0 15.0 40.0 16-17 34.88881621907083 40.0 37.0 40.0 18.5 40.0 18-19 34.96070626359169 40.0 37.0 40.0 18.5 40.0 20-21 34.96995064654483 40.0 37.0 40.0 18.5 40.0 22-23 35.86407071112836 40.0 37.0 40.0 27.0 40.0 24-25 34.98026978173258 40.0 37.0 40.0 18.5 40.0 26-27 34.94245231591131 38.5 37.0 40.0 21.0 40.0 28-29 34.62820498022778 37.0 37.0 40.0 15.0 40.0 30-31 34.53221569823228 37.0 37.0 40.0 15.0 40.0 32-33 34.331268534982144 37.0 37.0 40.0 15.0 40.0 34-35 34.68069158414127 37.0 37.0 40.0 18.5 40.0 36-37 34.30836637849557 37.0 37.0 40.0 15.0 40.0 38-39 34.23650500034154 37.0 37.0 40.0 15.0 40.0 40-41 34.01439136762033 37.0 35.0 40.0 15.0 40.0 42-43 33.929810164317885 37.0 33.0 40.0 15.0 40.0 44-45 33.788872113755716 37.0 33.0 40.0 15.0 40.0 46-47 33.72628435257247 37.0 33.0 40.0 15.0 40.0 48-49 33.5984783362684 37.0 33.0 40.0 15.0 40.0 50-51 32.68598123838326 37.0 33.0 38.5 15.0 38.5 52-53 33.1772072487497 37.0 33.0 38.5 15.0 40.0 54-55 33.729466319289585 37.0 33.0 40.0 15.0 40.0 56-57 34.08874321917433 37.0 33.0 40.0 18.5 40.0 58-59 33.44761235654754 37.0 33.0 40.0 15.0 40.0 60-61 33.27225375732813 37.0 33.0 40.0 15.0 40.0 62-63 32.78167855863616 37.0 33.0 40.0 6.0 40.0 64-65 32.57367625246006 37.0 33.0 37.0 10.5 40.0 66-67 32.46156947128434 37.0 33.0 37.0 15.0 40.0 68-69 32.669539137537825 37.0 33.0 37.0 15.0 40.0 70-71 32.1677041116274 37.0 33.0 37.0 15.0 38.5 72-73 32.53303481837728 37.0 33.0 37.0 18.5 37.0 74-75 32.66669303089402 37.0 33.0 37.0 22.0 37.0 76-77 32.332010170789104 37.0 33.0 37.0 22.0 37.0 78-79 31.81178212274541 37.0 33.0 37.0 22.0 37.0 80-81 31.318205564908126 33.0 33.0 37.0 15.0 37.0 82-83 30.904543931349068 33.0 33.0 37.0 15.0 37.0 84-85 30.670684183866644 33.0 33.0 37.0 15.0 37.0 86-87 30.496829007865557 33.0 27.0 37.0 15.0 37.0 88-89 30.36878192917648 33.0 27.0 37.0 15.0 37.0 90-91 30.213065940780016 33.0 27.0 37.0 15.0 37.0 92-93 30.059351583812038 33.0 27.0 37.0 6.0 37.0 94-95 29.938235427126028 33.0 27.0 37.0 6.0 37.0 96-97 29.740990104143112 33.0 27.0 37.0 6.0 37.0 98-99 29.630849287002178 33.0 27.0 37.0 6.0 37.0 100-101 28.46848875644544 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.2074608670903153 2306 2 -0.40654102692973026 2306 3 -0.46686135511346905 2306 4 -0.22880466697116475 2306 5 -0.3288548806942799 2306 6 -0.3954219421946661 2306 7 0.2788365367039063 2306 8 -0.5352769967649991 2306 9 -0.27657625759061233 2306 10-11 -0.24675609827338008 2306 12-13 -0.2503155857103323 2306 14-15 0.1760805684954292 2306 16-17 0.0690538167731134 2306 18-19 0.04872508879467574 2306 20-21 0.02910819172472401 2306 22-23 0.005057340073470584 2306 24-25 0.1932942118428329 2306 26-27 -0.1738265477546932 2306 28-29 -0.04017743856709899 2306 30-31 0.07520762542984727 2306 32-33 0.11702166714236739 2306 34-35 -0.05155369586579894 2306 36-37 0.33190321504094555 2306 38-39 0.04732293015400302 2306 40-41 0.19027047344976467 2306 42-43 0.1608555577738855 2306 44-45 0.20571115293493847 2306 46-47 0.20387165351363024 2306 48-49 0.18294322914893968 2306 50-51 -0.028651408170198067 2306 52-53 -0.03662866842211798 2306 54-55 0.176440994731756 2306 56-57 0.21784199501751544 2306 58-59 0.39236537459743914 2306 60-61 0.18136657484592433 2306 62-63 0.16950728207880417 2306 64-65 0.12581067632955723 2306 66-67 0.16545852962497065 2306 68-69 0.06773850147751403 2306 70-71 0.18928770377966941 2306 72-73 -0.219629975200327 2306 74-75 0.001725234614319504 2306 76-77 0.2587431225826009 2306 78-79 0.2917226746533004 2306 80-81 0.45656379079936826 2306 82-83 0.4104907853690385 2306 84-85 0.4001440665067868 2306 86-87 0.24282810578312208 2306 88-89 0.2556294662804035 2306 90-91 0.3717751903387665 2306 92-93 0.07143707066413896 2306 94-95 0.08121971898812674 2306 96-97 0.3015762975484826 2306 98-99 0.25203230368327567 2306 100-101 0.15463834228428297 2307 1 -0.1192648252833699 2307 2 -0.18536172486403046 2307 3 -0.1584885633878912 2307 4 -0.0441637891002884 2307 5 -0.08255194214846284 2307 6 -0.2677813114556784 2307 7 0.3349253501604963 2307 8 -0.2520516584607009 2307 9 0.04028814501162259 2307 10-11 0.011332284320630492 2307 12-13 0.04855159094315553 2307 14-15 0.3324775831289841 2307 16-17 0.27596672958834034 2307 18-19 0.3168008608577608 2307 20-21 0.24512361580818265 2307 22-23 0.04458184438649937 2307 24-25 0.45697368361460633 2307 26-27 0.06802561028423781 2307 28-29 0.30321932694291576 2307 30-31 0.37546004351326445 2307 32-33 0.29792963366852376 2307 34-35 0.009047556589962369 2307 36-37 0.3077739980938432 2307 38-39 0.10154410538975611 2307 40-41 0.22413654556149254 2307 42-43 0.1519280636795699 2307 44-45 0.2810603615735019 2307 46-47 0.17090315643896759 2307 48-49 0.1715288978564402 2307 50-51 0.18763484025991772 2307 52-53 -0.007096864673044934 2307 54-55 0.08301514267861165 2307 56-57 0.13882543386183244 2307 58-59 0.3222916184268243 2307 60-61 0.18334885802918421 2307 62-63 0.2816076491584454 2307 64-65 0.24881057193335465 2307 66-67 0.10926473905837497 2307 68-69 -0.019774400748715948 2307 70-71 0.07727285362639691 2307 72-73 -0.1378738849097232 2307 74-75 -0.007574451051844733 2307 76-77 0.11201329648610425 2307 78-79 0.20065084093305785 2307 80-81 0.38446252415302595 2307 82-83 0.4270709863043578 2307 84-85 0.3783930582701096 2307 86-87 0.2648962444750609 2307 88-89 0.29382777360126866 2307 90-91 0.3456110356816744 2307 92-93 0.41916661079136475 2307 94-95 0.5232792005953861 2307 96-97 0.46930332984308976 2307 98-99 0.3600419243997308 2307 100-101 0.19406807674779358 2201 1 -0.1297777169320007 2201 2 -0.3603163021958906 2201 3 -0.2932594778681725 2201 4 -0.17008697861418653 2201 5 -0.21274305954241868 2201 6 -0.16996910558079037 2201 7 0.2223461594456353 2201 8 -0.22047052279465618 2201 9 -0.05272909021414307 2201 10-11 -0.016812881301930815 2201 12-13 -0.026308571997098795 2201 14-15 0.33670750240946035 2201 16-17 0.20479450463924564 2201 18-19 0.23508693425666394 2201 20-21 0.05474512668865117 2201 22-23 0.06417809224230098 2201 24-25 0.3390826715261781 2201 26-27 0.09450594037402027 2201 28-29 0.2385253421625464 2201 30-31 0.36120909992126116 2201 32-33 0.249851183624628 2201 34-35 0.11854264196397679 2201 36-37 0.3500129216961838 2201 38-39 0.19370215493413667 2201 40-41 0.34301298486583676 2201 42-43 0.3778467274841262 2201 44-45 0.39499288655872533 2201 46-47 0.26355535457230417 2201 48-49 0.2919920582144684 2201 50-51 0.27205561937365275 2201 52-53 0.16839936279867374 2201 54-55 0.37224818580236985 2201 56-57 0.46295658965440367 2201 58-59 0.5545304905170312 2201 60-61 0.333205911843919 2201 62-63 0.32966126299253773 2201 64-65 0.3133038433441868 2201 66-67 0.2925881256429932 2201 68-69 0.17540384228997397 2201 70-71 0.3233415207792518 2201 72-73 0.24514808207393912 2201 74-75 0.34635982534610577 2201 76-77 0.50169250832095 2201 78-79 0.5697228055986727 2201 80-81 0.6558905068714402 2201 82-83 0.6395225372980597 2201 84-85 0.6650054949193738 2201 86-87 0.5153470492786418 2201 88-89 0.5392313086585894 2201 90-91 0.5242774673548674 2201 92-93 0.6111108849595759 2201 94-95 0.4624355274266634 2201 96-97 0.4564445018031229 2201 98-99 0.5969871294658766 2201 100-101 0.5166602705982726 1101 1 -0.08629596932181371 1101 2 -0.26316895413111396 1101 3 -0.2566075976383111 1101 4 -0.22173074768914347 1101 5 -0.19098912540316348 1101 6 -0.19450786956909383 1101 7 0.13654526309427695 1101 8 -0.21464927981745063 1101 9 -0.10653583392914356 1101 10-11 -0.027727196344685012 1101 12-13 -0.028716943442169907 1101 14-15 0.1767628598960087 1101 16-17 0.24346638321937775 1101 18-19 0.1752754155001952 1101 20-21 0.15160308524385613 1101 22-23 0.22331643096779885 1101 24-25 0.20804833888634988 1101 26-27 0.08027613348366458 1101 28-29 0.13658760100462075 1101 30-31 0.2676198909650864 1101 32-33 0.12979211425363246 1101 34-35 0.03806142593229822 1101 36-37 0.27643581073375145 1101 38-39 0.17701425567742746 1101 40-41 0.25916082784854666 1101 42-43 0.15261989010132737 1101 44-45 0.19558105985332475 1101 46-47 0.1279233260503787 1101 48-49 0.15685196894593645 1101 50-51 0.2188672939376275 1101 52-53 0.11416873104510472 1101 54-55 0.35977487929236673 1101 56-57 0.39564981386138953 1101 58-59 0.41219988534238894 1101 60-61 0.22093080163302403 1101 62-63 0.3808305065929787 1101 64-65 0.27112767089278833 1101 66-67 0.21806196373150755 1101 68-69 0.2712630289748432 1101 70-71 0.2680300890916669 1101 72-73 0.1768633851195176 1101 74-75 0.3298964721855029 1101 76-77 0.3956311384981106 1101 78-79 0.554009161444668 1101 80-81 0.5936520572476702 1101 82-83 0.43439486680341943 1101 84-85 0.47226360378298793 1101 86-87 0.4491089296001576 1101 88-89 0.4596464439819812 1101 90-91 0.39183032733892276 1101 92-93 0.47124318385334263 1101 94-95 0.47793676930405127 1101 96-97 0.49084656762720513 1101 98-99 0.4303393277263332 1101 100-101 0.4475035099277527 2304 1 -0.060814366884532234 2304 2 -0.17694934908128346 2304 3 -0.1747522405952573 2304 4 -0.03635158338045841 2304 5 -0.11682418886234558 2304 6 -0.08112597415765066 2304 7 0.19313005635865466 2304 8 0.005354801835942169 2304 9 0.04245153133334867 2304 10-11 0.013618295170331862 2304 12-13 0.10900810094914704 2304 14-15 0.15196010150158656 2304 16-17 0.2658364205446091 2304 18-19 0.25073455916592735 2304 20-21 0.20564994054404195 2304 22-23 0.0993604341915102 2304 24-25 0.1534307900510683 2304 26-27 0.15450802897979088 2304 28-29 0.2810208197597106 2304 30-31 0.19617356664276286 2304 32-33 0.1588136195103047 2304 34-35 0.07863906698826639 2304 36-37 0.2611867403563224 2304 38-39 0.27453964140818954 2304 40-41 0.30967077442896596 2304 42-43 0.296798761605217 2304 44-45 0.2271162780862923 2304 46-47 0.23455582530215224 2304 48-49 0.3075182075269254 2304 50-51 0.2700926791119045 2304 52-53 0.12176920758479781 2304 54-55 0.3303306616064461 2304 56-57 0.4118439309576658 2304 58-59 0.37666415684225996 2304 60-61 0.2247197288598315 2304 62-63 0.3039838548189877 2304 64-65 0.3677128488535928 2304 66-67 0.37960528236492763 2304 68-69 0.2863836555025969 2304 70-71 0.34371025174809233 2304 72-73 0.15988287834124293 2304 74-75 0.38305837446341684 2304 76-77 0.47915762654142924 2304 78-79 0.4652455395010975 2304 80-81 0.572472068672429 2304 82-83 0.5050478454547012 2304 84-85 0.37156178078157964 2304 86-87 0.32916200198187795 2304 88-89 0.37548687020627725 2304 90-91 0.5975724841890937 2304 92-93 0.6231485865155868 2304 94-95 0.509523461855867 2304 96-97 0.5900218232265324 2304 98-99 0.5136080510107952 2304 100-101 0.5377829628555482 2202 1 -0.00586330403720936 2202 2 -0.15652059110461636 2202 3 -0.16187306110423094 2202 4 -0.17380327652705319 2202 5 -0.1847355839559981 2202 6 0.027798070504495342 2202 7 -0.028496187482481616 2202 8 0.09218841551682999 2202 9 -0.13030591350787546 2202 10-11 -0.04346077939034387 2202 12-13 -0.04865890353219271 2202 14-15 -0.009934156072070266 2202 16-17 0.11844357004166994 2202 18-19 0.09158672378189436 2202 20-21 0.06545739721889987 2202 22-23 0.12965843748064998 2202 24-25 0.24967089628339068 2202 26-27 0.27326082532940177 2202 28-29 0.14840317722794083 2202 30-31 0.20122677383164955 2202 32-33 0.04285416609700832 2202 34-35 0.09057696278724592 2202 36-37 0.1612342127872779 2202 38-39 0.2353292891632961 2202 40-41 0.21295390265528624 2202 42-43 0.2818653030027747 2202 44-45 0.2650838236043782 2202 46-47 0.10511009144897798 2202 48-49 0.30375938954356485 2202 50-51 0.3401788411603306 2202 52-53 0.2636559181177134 2202 54-55 0.21094569025816412 2202 56-57 0.26536660049161753 2202 58-59 0.302301696970396 2202 60-61 0.258765461278621 2202 62-63 0.3852975088390238 2202 64-65 0.30870922373629384 2202 66-67 0.34393307439761145 2202 68-69 0.3201671999746907 2202 70-71 0.3326170397264576 2202 72-73 0.3658585016203375 2202 74-75 0.5245509065893685 2202 76-77 0.4352384560097491 2202 78-79 0.4599145317008322 2202 80-81 0.3681531256553434 2202 82-83 0.3609102079542943 2202 84-85 0.39081492717485844 2202 86-87 0.38323158371142085 2202 88-89 0.18901769915050082 2202 90-91 0.24908065104710175 2202 92-93 0.3004904172577483 2202 94-95 0.40955441134310533 2202 96-97 0.36632069486670815 2202 98-99 0.2967897907029915 2202 100-101 0.2218649685147227 1102 1 -0.055223666326348564 1102 2 -0.12280958482665838 1102 3 -0.11182374029747422 1102 4 -0.2391101557783415 1102 5 -0.28745295026941875 1102 6 -0.05258337340105612 1102 7 -0.1558166510931187 1102 8 0.07085764867841249 1102 9 -0.14960975588302716 1102 10-11 0.05562677332358135 1102 12-13 -0.048594477455793594 1102 14-15 -0.07487957190704009 1102 16-17 -0.05722278001591263 1102 18-19 0.06882122827218495 1102 20-21 -0.0030282883749279677 1102 22-23 0.19333157427901426 1102 24-25 0.13367879156802331 1102 26-27 0.18601367790148515 1102 28-29 0.046566242940066616 1102 30-31 0.0988356417868701 1102 32-33 0.05000981168534935 1102 34-35 0.09284967545139722 1102 36-37 -0.12645706912352495 1102 38-39 0.04254486138601976 1102 40-41 0.007111352470438703 1102 42-43 0.0747352449006442 1102 44-45 0.10627431168506263 1102 46-47 0.04141186160858723 1102 48-49 0.09059686524781085 1102 50-51 -0.03639852229520102 1102 52-53 0.06884824948628676 1102 54-55 0.2278871772397011 1102 56-57 0.34961274370341755 1102 58-59 0.10091463271786694 1102 60-61 0.15058154485551256 1102 62-63 0.24787666317137536 1102 64-65 0.19794255342722522 1102 66-67 0.1372040599682478 1102 68-69 0.18039806585028373 1102 70-71 0.20329031941918174 1102 72-73 0.40540370197652464 1102 74-75 0.43484427020905514 1102 76-77 0.39594312301890255 1102 78-79 0.3542253333777836 1102 80-81 0.27835071543775314 1102 82-83 0.21824182622559007 1102 84-85 0.2757893866608434 1102 86-87 0.24952863687356341 1102 88-89 0.20366135570364108 1102 90-91 0.2544188187259806 1102 92-93 0.3515559197391198 1102 94-95 0.2501384389172685 1102 96-97 0.25886872888248647 1102 98-99 0.3270285132965647 1102 100-101 0.33615314442609545 2305 1 0.0926391959825601 2305 2 0.08140881851486625 2305 3 0.07423467628135683 2305 4 -0.053870427490657846 2305 5 -0.03530240860760614 2305 6 0.32191505812603793 2305 7 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1213 60-61 -0.15760547600909547 1213 62-63 -0.3151186022971686 1213 64-65 -0.21698595545186805 1213 66-67 -0.012666104720096882 1213 68-69 0.10481842959163146 1213 70-71 -0.12187948176280372 1213 72-73 0.146260046940057 1213 74-75 -3.6938327368574164E-4 1213 76-77 -0.12153698820360859 1213 78-79 -0.17083263523135273 1213 80-81 -0.524465567032351 1213 82-83 -0.48396703953577 1213 84-85 -0.40955835627724824 1213 86-87 -0.43576387038864084 1213 88-89 -0.24654942636323796 1213 90-91 -0.3018115189732953 1213 92-93 -0.27780489228511485 1213 94-95 -0.18580131716240444 1213 96-97 -0.2264670515713867 1213 98-99 -0.28313352131926095 1213 100-101 -0.05874567829340194 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1935.0 3 600.0 4 741.0 5 781.0 6 2037.0 7 6434.0 8 25449.0 9 88695.0 10 133741.0 11 102025.0 12 63280.0 13 42241.0 14 30361.0 15 23481.0 16 20675.0 17 19382.0 18 19060.0 19 19690.0 20 20322.0 21 21541.0 22 23012.0 23 24537.0 24 26498.0 25 29592.0 26 35015.0 27 42831.0 28 53576.0 29 66989.0 30 77972.0 31 99774.0 32 137904.0 33 199631.0 34 305730.0 35 522123.0 36 1032419.0 37 1623211.0 38 341588.0 39 66.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.61323722363182 23.835514489474424 22.473374545242127 14.077873741651631 2 30.236942916745686 27.441247866489665 29.60498767305139 12.716821543713255 3 34.31338976886914 19.843867498978952 29.82480789657606 16.01793483557585 4 27.366485032479726 17.58426973747948 33.17615002385998 21.873095206180807 5 34.640828460704235 16.460745948323588 30.960931752857217 17.937493838114957 6 31.381313108348564 16.9893320177393 35.48405675848543 16.145298115426705 7 34.799334486465845 20.26531897145429 27.645575403274382 17.289771138805484 8 27.033459065653865 32.047574357425134 23.812426191844857 17.106540385076144 9 32.51047808078302 26.635120367722397 23.07452363320941 17.779877918285177 10-11 26.82363965975541 29.95463859899999 26.447357408681683 16.774364332562918 12-13 28.904999619292372 23.83744011431078 26.154860500011175 21.102699766385673 14-15 25.166218140023656 24.189318398608357 28.667927011380606 21.976536449987385 16-17 27.36127955224535 25.233016943355203 29.633187055880033 17.77251644851942 18-19 26.787273133754702 25.42650887257955 29.961083009498534 17.825134984167217 20-21 25.25534754649336 28.714109978211 28.18496070367148 17.845581771624154 22-23 29.077572367643633 25.686705265522942 27.289926471765785 17.94579589506764 24-25 24.2039464551918 30.316450646650996 27.216475038515725 18.26312785964148 26-27 22.895765671799804 31.681971762916362 26.88935846777812 18.53290409750572 28-29 24.228678739772544 29.948253649526595 26.775383571943557 19.047684038757303 30-31 26.82805378849478 26.60243562090833 27.688105612154644 18.881404978442237 32-33 22.360451055825273 29.406330461173148 29.659600553248666 18.573617929752913 34-35 27.93677349800846 26.61723236170413 27.74156364741908 17.70443049286833 36-37 23.509071001898743 27.05435310695366 30.002834539768898 19.4337413513787 38-39 20.24278683668897 29.939915621748476 31.286360115137203 18.530937426425353 40-41 22.21251124407535 30.830663631854136 27.951999962025287 19.004825162045222 42-43 25.12255995818889 26.975250238055175 29.765224611277834 18.1369651924781 44-45 22.03692445215394 29.594528602523578 30.13893804368794 18.22960890163454 46-47 24.121146848169232 30.39057628989463 27.196005262151346 18.29227159978479 48-49 20.901190921078218 29.0508520793836 29.843875328131837 20.20408167140635 50-51 19.76374932227836 26.856615465251753 31.505752681688744 21.873882530781145 52-53 26.266809832569383 26.41372926699887 27.309886546180827 20.009574354250915 54-55 27.554012719976452 26.51488650377596 27.308658068779994 18.622442707467595 56-57 28.014528796706212 26.11302377757069 26.934581110855415 18.937866314867684 58-59 25.88944040134176 25.90434414782086 27.230711134636458 20.975504316200926 60-61 21.37545644576155 26.474561230760468 26.431084104654502 25.718898218823476 62-63 24.866572467268238 26.624536317853696 27.09527392950988 21.41361728536819 64-65 21.72571272618644 28.489764839360227 30.056449105844496 19.72807332860884 66-67 20.41917473076719 28.283588697702932 28.16426079481893 23.13297577671095 68-69 19.944793328505796 26.344259198976538 34.287737133865505 19.423210338652165 70-71 19.550277079505435 31.596714122405384 30.210514758959 18.64249403913018 72-73 19.47035089177513 35.92204876193686 26.25528455469692 18.352315791591092 74-75 20.34164254874982 35.49841058974238 25.640366276035486 18.519580585472315 76-77 20.793514706587064 34.49642992872193 26.047443113479822 18.66261225121118 78-79 20.4220041353194 34.151040600584494 26.86537038666062 18.561584877435482 80-81 19.65178716412406 34.54940068173823 26.60519627757301 19.193615876564703 82-83 19.001149331774688 34.106370575056054 26.975653095653513 19.916826997515745 84-85 19.19389434761867 33.10610931621318 27.468316871717857 20.231679464450295 86-87 19.17388339299228 32.79433972222613 28.0698884902344 19.9618883945472 88-89 19.614044800466594 33.181475228817135 27.568468573147058 19.636011397569206 90-91 20.252242952618573 32.04106979880244 27.35813742298867 20.34854982559031 92-93 19.895475306382743 32.6555593838934 26.786521370333233 20.66244393939063 94-95 19.594532851069943 32.968630896468454 26.73372515256876 20.70311109989284 96-97 20.30803631122076 33.11152147780599 26.513042660474404 20.067399550498845 98-99 20.774160455128087 31.903891627635726 26.56316443719512 20.758783480041064 100-101 20.765395977662394 31.90469694825232 26.085315127284858 21.24459194680043 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1126.0 1 818.5 2 404.5 3 298.5 4 306.5 5 389.0 6 560.5 7 757.0 8 1050.5 9 1437.0 10 1733.5 11 1871.0 12 1867.5 13 1758.0 14 1586.5 15 1432.0 16 1243.0 17 1027.5 18 979.0 19 1025.0 20 1086.0 21 1209.5 22 1431.5 23 1710.5 24 1997.0 25 2404.5 26 3034.0 27 3794.5 28 4652.5 29 5820.5 30 7606.5 31 11231.5 32 20536.5 33 38733.5 34 68728.5 35 110273.5 36 167245.5 37 244574.0 38 317413.0 39 345659.0 40 350188.5 41 334046.5 42 316809.5 43 342161.5 44 350344.5 45 336738.5 46 345853.5 47 323565.0 48 258147.5 49 207706.5 50 172732.5 51 142876.0 52 112951.5 53 82824.5 54 61615.0 55 46288.5 56 34722.5 57 25077.0 58 16942.0 59 11697.5 60 8470.0 61 6235.5 62 4413.5 63 3014.5 64 2160.5 65 1633.5 66 1249.5 67 944.0 68 724.0 69 559.0 70 402.0 71 299.0 72 241.0 73 182.5 74 146.5 75 126.0 76 107.0 77 91.0 78 80.0 79 65.5 80 51.5 81 38.0 82 31.0 83 28.5 84 27.0 85 18.5 86 11.5 87 12.0 88 9.0 89 5.5 90 4.5 91 5.0 92 4.5 93 1.5 94 2.0 95 2.0 96 1.0 97 1.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23373968933227043 2 0.22590610790398907 3 0.20732500412965976 4 0.197126210917477 5 0.200172603695142 6 0.12705917703118239 7 0.10230960092443829 8 0.09846849698738244 9 0.09212972940652674 10-11 0.07184567314778846 12-13 0.10022821455460508 14-15 0.10276372158694737 16-17 0.09280144955315474 18-19 0.08699248941189293 20-21 0.08123083350630915 22-23 0.1614115129805661 24-25 0.6279731894729532 26-27 0.35935135675170515 28-29 0.17965202625801358 30-31 0.3084803817035542 32-33 0.3449330257170423 34-35 0.3268628076880358 36-37 0.3362385071994209 38-39 0.3439774801563462 40-41 0.3458223453477893 42-43 0.366191549230748 44-45 0.34306923883132806 46-47 0.36730792919274946 48-49 0.35502774961073347 50-51 0.36204769818535276 52-53 0.3554913311203781 54-55 0.33812121577940635 56-57 0.34617239669180666 58-59 0.3369386098874556 60-61 0.31507459215707123 62-63 0.30225514428832573 64-65 0.3017064151544606 66-67 0.3156611646794788 68-69 0.3132297269656282 70-71 0.2986505615296601 72-73 0.2902020250375643 74-75 0.29686246142103057 76-77 0.2987073266124737 78-79 0.30158342414169775 80-81 0.3121038861564911 82-83 0.33993769842944266 84-85 0.3260207922929669 86-87 0.3363709590593193 88-89 0.33777116443538896 90-91 0.3194549643808566 92-93 0.2980829107015237 94-95 0.2981585974786085 96-97 0.30671120328919593 98-99 0.3277805098601895 100-101 0.33450717217360504 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5284939.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.74198285539819 #Duplication Level Percentage of deduplicated Percentage of total 1 86.03233779185206 28.16869328990646 2 5.789050134633393 3.790899605144143 3 2.130917849161445 2.0931142705051804 4 1.1505012644368422 1.5067877070122009 5 0.6562355570657398 1.0743226679274567 6 0.6105274921732168 1.1993928408890833 7 0.36123712048809037 0.8279333724028125 8 0.3480125646564525 0.9115697140355778 9 0.27444544158328227 0.8087299148755812 >10 2.057093812655525 13.415792838894399 >50 0.27215498113324954 6.1953689984884175 >100 0.25369241582638585 17.95174742368672 >500 0.042937944603127366 9.59362296723037 >1k 0.02044785962394505 10.823759312271815 >5k 3.495186160897309E-4 0.8262070154307337 >10k+ 5.825149122115617E-5 0.8120580612990869 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT 42577 0.805628976985354 Illumina Single End PCR Primer 1 (97% over 34bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 8876 0.16794895835126952 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 8474 0.16034243725424266 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 7999 0.1513546324754174 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 7827 0.1481001010607691 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.005846803529804223 0.0 0.0 2 0.006282002498042077 0.0 0.0 3 0.0069064184089920435 0.0 0.0 4 0.007398382460043531 0.0 0.0 5 0.008533684116316196 0.0 0.0 6 0.009593298995504016 0.0 0.0 7 0.010917817594488791 1.892169427121108E-5 0.0 8 0.012374788053372044 1.892169427121108E-5 0.0 9 0.01369930665235682 1.892169427121108E-5 0.0 10-11 0.01954611018216104 1.892169427121108E-5 0.0 12-13 0.02526046185206679 1.892169427121108E-5 0.0 14-15 0.030747753190718 1.892169427121108E-5 0.0 16-17 0.03533626405148669 1.892169427121108E-5 0.0 18-19 0.040899242167222745 1.892169427121108E-5 0.0 20-21 0.04724747059521406 1.892169427121108E-5 0.0 22-23 0.053671385800290225 1.892169427121108E-5 0.0 24-25 0.06049265658506181 1.892169427121108E-5 0.0 26-27 0.06724770143988416 1.892169427121108E-5 0.0 28-29 0.07487314423118224 1.892169427121108E-5 0.0 30-31 0.11808083309949272 1.892169427121108E-5 0.0 32-33 0.12941492796794818 1.892169427121108E-5 0.0 34-35 0.1394339650845544 1.892169427121108E-5 0.0 36-37 0.15026663505482277 1.892169427121108E-5 0.0 38-39 0.1623292151527198 1.892169427121108E-5 0.0 40-41 0.17520542810427897 2.8382541406816616E-5 0.0 42-43 0.18947238558477214 3.784338854242216E-5 0.0 44-45 0.2048273404858599 3.784338854242216E-5 0.0 46-47 0.2206931811322704 3.784338854242216E-5 0.0 48-49 0.2375713324221907 3.784338854242216E-5 0.0 50-51 0.2557740023110957 3.784338854242216E-5 0.0 52-53 0.2754904077416977 3.784338854242216E-5 0.0 54-55 0.29607721110877533 3.784338854242216E-5 9.46084713560554E-6 56-57 0.31938873845090743 3.784338854242216E-5 1.892169427121108E-5 58-59 0.3443937574303128 3.784338854242216E-5 1.892169427121108E-5 60-61 0.3705624606073977 3.784338854242216E-5 1.892169427121108E-5 62-63 0.3984814205045697 3.784338854242216E-5 1.892169427121108E-5 64-65 0.42724239579681056 3.784338854242216E-5 1.892169427121108E-5 66-67 0.459513345376361 3.784338854242216E-5 1.892169427121108E-5 68-69 0.49263577119811597 3.784338854242216E-5 1.892169427121108E-5 70-71 0.5289086591160277 3.784338854242216E-5 1.892169427121108E-5 72-73 0.5680576445631633 3.784338854242216E-5 1.892169427121108E-5 74-75 0.6110004297116769 3.784338854242216E-5 1.892169427121108E-5 76-77 0.6581627526826705 3.784338854242216E-5 1.892169427121108E-5 78-79 0.7088445107881094 3.784338854242216E-5 1.892169427121108E-5 80-81 0.7643986051683851 3.784338854242216E-5 1.892169427121108E-5 82-83 0.8230747791034106 3.784338854242216E-5 1.892169427121108E-5 84-85 0.8912591044097198 3.784338854242216E-5 1.892169427121108E-5 86-87 0.9610517737290818 3.784338854242216E-5 1.892169427121108E-5 88-89 1.038299590591301 3.784338854242216E-5 1.892169427121108E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 5695 0.0 91.45079 9 CGGGAGA 7325 0.0 90.020035 4 AGAGCGG 6050 0.0 88.67208 8 ATTCGGA 1200 0.0 88.563 2 GAGAGCG 6920 0.0 87.38488 7 AAGCGGC 2120 0.0 86.955536 9 CGGAAAG 2980 0.0 86.437706 5 CGGAGAG 2085 0.0 84.40877 5 TCGGGAG 8090 0.0 84.26925 3 CCGGAGA 1060 0.0 83.241356 4 TTCGGAG 815 0.0 80.91296 3 AAAGCGG 2500 0.0 79.806435 8 GATCCGA 340 0.0 79.55283 1 AAGGGGC 4755 0.0 79.43124 9 TCGGAGA 1320 0.0 79.42375 4 TCGGGAA 2565 0.0 79.162094 3 GTTCGGA 810 0.0 79.07411 2 GGAAAGC 2585 0.0 78.10925 6 GATCCGG 1490 0.0 77.70755 1 GTCCGGA 410 0.0 76.37402 2 >>END_MODULE