FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n02_34042-1.34200000035847.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n02_34042-1.34200000035847.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5284939
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT425770.805628976985354Illumina Single End PCR Primer 1 (97% over 34bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC88760.16794895835126952No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG84740.16034243725424266No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC79990.1513546324754174No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG78270.1481001010607691No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC56950.091.450799
CGGGAGA73250.090.0200354
AGAGCGG60500.088.672088
ATTCGGA12000.088.5632
GAGAGCG69200.087.384887
AAGCGGC21200.086.9555369
CGGAAAG29800.086.4377065
CGGAGAG20850.084.408775
TCGGGAG80900.084.269253
CCGGAGA10600.083.2413564
TTCGGAG8150.080.912963
AAAGCGG25000.079.8064358
GATCCGA3400.079.552831
AAGGGGC47550.079.431249
TCGGAGA13200.079.423754
TCGGGAA25650.079.1620943
GTTCGGA8100.079.074112
GGAAAGC25850.078.109256
GATCCGG14900.077.707551
GTCCGGA4100.076.374022
Produced by FastQC (version 0.11.2)