##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n02_34012-1.34200000035798.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2838360 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.811253329387394 33.0 33.0 33.0 22.0 33.0 2 30.2111754675235 33.0 33.0 33.0 22.0 33.0 3 30.02160825265294 33.0 33.0 33.0 15.0 33.0 4 33.22967065488522 37.0 37.0 37.0 15.0 37.0 5 33.362436759255345 37.0 37.0 37.0 15.0 37.0 6 34.12782029059034 37.0 37.0 37.0 27.0 37.0 7 33.239308262517795 37.0 37.0 37.0 15.0 37.0 8 33.82272615172142 37.0 37.0 37.0 22.0 37.0 9 33.01063712848264 37.0 37.0 37.0 15.0 37.0 10-11 33.083888583548244 37.0 37.0 37.0 15.0 37.0 12-13 33.05979843994419 37.0 37.0 37.0 15.0 37.0 14-15 34.51033272734959 40.0 37.0 40.0 15.0 40.0 16-17 34.41567031666173 40.0 37.0 40.0 15.0 40.0 18-19 34.48413062472696 40.0 37.0 40.0 15.0 40.0 20-21 34.490092342056684 40.0 37.0 40.0 15.0 40.0 22-23 35.49821516650461 40.0 37.0 40.0 24.5 40.0 24-25 34.541511647571134 38.5 37.0 40.0 15.0 40.0 26-27 34.50790315534323 37.0 37.0 40.0 18.5 40.0 28-29 34.09959589340323 37.0 37.0 40.0 15.0 40.0 30-31 34.01631804985978 37.0 37.0 40.0 10.5 40.0 32-33 33.89031095421299 37.0 33.0 40.0 10.5 40.0 34-35 34.02476447666963 37.0 33.0 40.0 15.0 40.0 36-37 33.8486640172494 37.0 33.0 40.0 10.5 40.0 38-39 33.64720031990304 37.0 33.0 40.0 15.0 40.0 40-41 33.66962911681394 37.0 33.0 40.0 15.0 40.0 42-43 33.5362346566327 37.0 33.0 40.0 15.0 40.0 44-45 33.33636131428008 37.0 33.0 40.0 6.0 40.0 46-47 33.25879662903931 37.0 33.0 40.0 10.5 40.0 48-49 33.14364756408631 37.0 33.0 40.0 10.5 40.0 50-51 32.306272812469174 37.0 33.0 38.5 15.0 38.5 52-53 32.76822742710579 37.0 33.0 38.5 15.0 40.0 54-55 33.28994789244493 37.0 33.0 40.0 10.5 40.0 56-57 33.69461114869149 37.0 33.0 40.0 18.5 40.0 58-59 33.02359795797573 37.0 33.0 40.0 6.0 40.0 60-61 32.90097679645993 37.0 33.0 40.0 10.5 40.0 62-63 32.42046921461689 37.0 33.0 40.0 6.0 40.0 64-65 32.275491128679946 37.0 33.0 40.0 6.0 40.0 66-67 32.242343994419315 37.0 33.0 37.0 10.5 40.0 68-69 32.50292757085077 37.0 33.0 37.0 15.0 40.0 70-71 31.974927599036064 37.0 33.0 37.0 15.0 40.0 72-73 32.444280852323175 37.0 33.0 37.0 18.5 40.0 74-75 32.626564459758455 37.0 33.0 37.0 22.0 37.0 76-77 32.24680907284488 37.0 33.0 37.0 22.0 37.0 78-79 31.631504284164095 37.0 33.0 37.0 18.5 37.0 80-81 31.053743006524897 37.0 27.0 37.0 15.0 37.0 82-83 30.607257183725814 33.0 27.0 37.0 15.0 37.0 84-85 30.343691603602082 33.0 27.0 37.0 10.5 37.0 86-87 30.138964049662484 33.0 27.0 37.0 6.0 37.0 88-89 29.978917755323497 33.0 27.0 37.0 6.0 37.0 90-91 29.803557864400567 33.0 27.0 37.0 6.0 37.0 92-93 29.649585852393635 33.0 27.0 37.0 6.0 37.0 94-95 29.526658704322216 33.0 27.0 37.0 6.0 37.0 96-97 29.33626830282276 33.0 27.0 37.0 6.0 37.0 98-99 29.22505231894474 33.0 27.0 37.0 6.0 37.0 100-101 28.082756591834723 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.3108577628520095 2306 2 -0.48817236889108884 2306 3 -0.44175389950943966 2306 4 -0.2877351722372481 2306 5 -0.44386202374930406 2306 6 -0.5401615187114643 2306 7 0.20372416254593162 2306 8 -0.48929686273353923 2306 9 -0.1317710740858189 2306 10-11 -0.22960442201205922 2306 12-13 -0.17367683596001626 2306 14-15 0.23072492427968427 2306 16-17 0.21403412486004214 2306 18-19 0.2773791326472477 2306 20-21 0.12844785371813572 2306 22-23 -0.0034294629998470327 2306 24-25 0.2851902762130223 2306 26-27 -0.12423704679118686 2306 28-29 0.035668143228008375 2306 30-31 0.20010629116472245 2306 32-33 0.3926733647406877 2306 34-35 0.24897032481670323 2306 36-37 0.1925461858116435 2306 38-39 0.22457619643873983 2306 40-41 0.3402288047740356 2306 42-43 0.2922785709838749 2306 44-45 0.42673411433010955 2306 46-47 0.28891273270873796 2306 48-49 0.3754722936066628 2306 50-51 0.26866201439199955 2306 52-53 0.198054376454607 2306 54-55 0.3872528038955636 2306 56-57 0.44043393875494274 2306 58-59 0.6549777420476701 2306 60-61 0.37904558712999403 2306 62-63 0.3994050321912255 2306 64-65 0.28551008574588366 2306 66-67 0.2942221734669985 2306 68-69 0.13948796583466105 2306 70-71 0.3718472020390706 2306 72-73 0.05124334530746921 2306 74-75 0.29765878871373275 2306 76-77 0.548311219002386 2306 78-79 0.6301030442778277 2306 80-81 0.5070169162246678 2306 82-83 0.43987240538694294 2306 84-85 0.5471458576034074 2306 86-87 0.4932423317931338 2306 88-89 0.5361511147378124 2306 90-91 0.46527907239508437 2306 92-93 0.40383233223749926 2306 94-95 0.2635318163062408 2306 96-97 0.599394433546653 2306 98-99 0.38383488690032763 2306 100-101 0.43315134736866767 2307 1 -0.37371481176822385 2307 2 -0.5853842021600322 2307 3 -0.4554632079202463 2307 4 -0.2897271113228186 2307 5 -0.46709537653623556 2307 6 -0.5509664867774191 2307 7 -0.0020880800706066793 2307 8 -0.5742542977078244 2307 9 -0.3576246350536749 2307 10-11 -0.36009287177930105 2307 12-13 -0.3175142915386786 2307 14-15 0.010230693231186194 2307 16-17 -0.16447608554023674 2307 18-19 -0.12725644113806567 2307 20-21 -0.19278794309859393 2307 22-23 -0.3036683860923688 2307 24-25 -0.09018132865421791 2307 26-27 -0.4104186886202612 2307 28-29 -0.11797351597231653 2307 30-31 -0.03310196373772101 2307 32-33 -0.0236603987638091 2307 34-35 -0.11810199051793546 2307 36-37 -0.14195778847673068 2307 38-39 -0.12333363749328186 2307 40-41 -0.06493352319174761 2307 42-43 -0.07027968706950816 2307 44-45 -0.093451736052522 2307 46-47 -0.1597701763443098 2307 48-49 -0.05514342654643656 2307 50-51 -0.2295967513729522 2307 52-53 -0.2948409494754003 2307 54-55 -0.27701024714137645 2307 56-57 0.08886901989837526 2307 58-59 0.05751177899855264 2307 60-61 -0.07038738151683077 2307 62-63 0.24986369877021275 2307 64-65 0.030208294644673117 2307 66-67 0.01576237803120506 2307 68-69 -0.3132904740961848 2307 70-71 -0.04487563347388601 2307 72-73 -0.38938275923234755 2307 74-75 -0.13779978196722453 2307 76-77 -0.01071812853481191 2307 78-79 0.17188102115818182 2307 80-81 0.19745190968989945 2307 82-83 0.21838562972531506 2307 84-85 0.05500401619682904 2307 86-87 0.11078112254637063 2307 88-89 0.15017044778070954 2307 90-91 0.18879888629810537 2307 92-93 0.018488702195661233 2307 94-95 0.10757421967527492 2307 96-97 0.047345777626045304 2307 98-99 0.11344285575264834 2307 100-101 -0.05496551671094707 2201 1 -0.18176140749913472 2201 2 -0.43111982267585347 2201 3 -0.21628156633671836 2201 4 -0.1463845279929714 2201 5 -0.24890934263815012 2201 6 -0.20189136353289427 2201 7 0.3656365600684808 2201 8 -0.2695521205585081 2201 9 0.022750133184338495 2201 10-11 0.051020110086938075 2201 12-13 0.04354178933038355 2201 14-15 0.3837830769692161 2201 16-17 0.2748829773671062 2201 18-19 0.32560749378914977 2201 20-21 0.1472143591591717 2201 22-23 0.13183111781614798 2201 24-25 0.27352972242886864 2201 26-27 0.016174723645285383 2201 28-29 0.2816666845617206 2201 30-31 0.4303822617000179 2201 32-33 0.40087880147608956 2201 34-35 0.3659036676287357 2201 36-37 0.32302945898157276 2201 38-39 0.3958369598074327 2201 40-41 0.44855803505988234 2201 42-43 0.44055239476961816 2201 44-45 0.34361141872884104 2201 46-47 0.4008355304693083 2201 48-49 0.3618620243151014 2201 50-51 0.4030989139099077 2201 52-53 0.21989882575542197 2201 54-55 0.494777222513477 2201 56-57 0.5230475931703964 2201 58-59 0.7112055098932899 2201 60-61 0.49672076970463763 2201 62-63 0.5192958604100113 2201 64-65 0.4782038965949553 2201 66-67 0.40124983810013504 2201 68-69 0.2381563843051353 2201 70-71 0.572021504762354 2201 72-73 0.4366818711241933 2201 74-75 0.42071422944246706 2201 76-77 0.5524798713685044 2201 78-79 0.6143469030908655 2201 80-81 0.796045893833 2201 82-83 0.8100791902038225 2201 84-85 0.7428375260912112 2201 86-87 0.611170499060556 2201 88-89 0.6580704281992311 2201 90-91 0.6591978733046844 2201 92-93 0.554022739970712 2201 94-95 0.5915333820467161 2201 96-97 0.6138900395557947 2201 98-99 0.5975560481812501 2201 100-101 0.5508045885128858 1101 1 -0.07237658138633307 1101 2 -0.29016221548776855 1101 3 -0.17481913923839798 1101 4 -0.1360512262467779 1101 5 -0.20935506588313046 1101 6 -0.16901345931083966 1101 7 0.3081637609023815 1101 8 -0.28425306214240464 1101 9 -0.15570284361633924 1101 10-11 -0.0983734168468402 1101 12-13 -0.07023867431139053 1101 14-15 0.15232831693096216 1101 16-17 0.170516198996836 1101 18-19 0.1706316579503806 1101 20-21 0.0909174630263081 1101 22-23 0.0908403441322605 1101 24-25 0.25709723132352025 1101 26-27 0.03611338716472545 1101 28-29 0.20087826957563237 1101 30-31 0.3658282003118458 1101 32-33 0.3290776453867963 1101 34-35 0.24714176860994286 1101 36-37 0.2885772230428785 1101 38-39 0.1426624523514235 1101 40-41 0.3445853780359087 1101 42-43 0.27938045448653526 1101 44-45 0.3024311222117504 1101 46-47 0.21948156211961134 1101 48-49 0.1648638075526705 1101 50-51 0.17438792761584665 1101 52-53 0.23112589995361077 1101 54-55 0.4053073695659677 1101 56-57 0.5080817503537389 1101 58-59 0.5478002153145027 1101 60-61 0.27221805177491376 1101 62-63 0.3955999067225022 1101 64-65 0.39879382948664244 1101 66-67 0.29208683984891337 1101 68-69 0.29155874989467634 1101 70-71 0.36221111374229054 1101 72-73 0.3400403018751348 1101 74-75 0.5462838747763357 1101 76-77 0.5656926279781231 1101 78-79 0.714333119612089 1101 80-81 0.763911005263811 1101 82-83 0.5927955692839468 1101 84-85 0.6090279448505278 1101 86-87 0.4923655266705822 1101 88-89 0.5761664128995676 1101 90-91 0.5836730242768553 1101 92-93 0.5842602465394009 1101 94-95 0.5927200534227381 1101 96-97 0.531766350063112 1101 98-99 0.5919522025906723 1101 100-101 0.513604271492877 2304 1 0.17091480191496444 2304 2 0.17287544308592473 2304 3 0.20029199292848787 2304 4 0.4307159230420936 2304 5 0.2993977265868253 2304 6 0.30156116279718503 2304 7 0.6753936903520952 2304 8 0.36586478865308436 2304 9 0.5195840039076813 2304 10-11 0.4325505091908113 2304 12-13 0.39399819769281663 2304 14-15 0.6623299137236103 2304 16-17 0.6542405022660986 2304 18-19 0.6092966711328671 2304 20-21 0.5165657190884545 2304 22-23 0.3533880753659844 2304 24-25 0.6662082029590621 2304 26-27 0.6514868534470892 2304 28-29 0.7746333890683772 2304 30-31 0.7259938134418746 2304 32-33 0.7913946170772235 2304 34-35 0.5915154092273056 2304 36-37 0.6840564082457661 2304 38-39 0.7188300579787494 2304 40-41 0.7390517842964215 2304 42-43 0.7276211110767647 2304 44-45 0.6461968909651645 2304 46-47 0.6996947961393758 2304 48-49 0.8170985589071762 2304 50-51 0.5634715918817648 2304 52-53 0.3899752412894202 2304 54-55 0.771306473521598 2304 56-57 0.7073457090395863 2304 58-59 0.7040847191150235 2304 60-61 0.5816381229301726 2304 62-63 0.7306245564633969 2304 64-65 0.7450403357571673 2304 66-67 0.5690338234360368 2304 68-69 0.5344080755522711 2304 70-71 0.6006848526382313 2304 72-73 0.36108907937443746 2304 74-75 0.501015825779163 2304 76-77 0.6089827502051861 2304 78-79 0.7236776796871958 2304 80-81 0.7678440231354493 2304 82-83 0.7732776166141093 2304 84-85 0.6433768803419788 2304 86-87 0.6214173880076359 2304 88-89 0.8841106011622735 2304 90-91 0.7663320436075871 2304 92-93 0.8270531646873316 2304 94-95 0.7326451865129329 2304 96-97 0.7920109166651308 2304 98-99 0.9032749621005784 2304 100-101 0.8099769959568448 2202 1 0.10095143430802977 2202 2 -0.16907344039256245 2202 3 -0.07685503920750492 2202 4 -0.17888933613127023 2202 5 -0.10009776406867132 2202 6 0.1560470736747348 2202 7 0.023830086384869276 2202 8 0.1606151236825042 2202 9 0.0368805593455761 2202 10-11 0.16121010440028272 2202 12-13 0.04912266106128271 2202 14-15 0.04003645008040735 2202 16-17 0.1380485514762384 2202 18-19 0.20406625829158287 2202 20-21 0.27471166842946104 2202 22-23 0.3408996097763719 2202 24-25 0.22653428727209501 2202 26-27 0.2629459341995002 2202 28-29 0.2427772343258212 2202 30-31 0.28465247821021933 2202 32-33 0.33267074297767607 2202 34-35 0.1423237711472538 2202 36-37 0.27569701445405315 2202 38-39 0.12879371742725 2202 40-41 0.15684887940586378 2202 42-43 0.14665453180043642 2202 44-45 0.1692373905728246 2202 46-47 0.06846023255784672 2202 48-49 0.14899185284293992 2202 50-51 0.2254923029051028 2202 52-53 0.22402165131647678 2202 54-55 0.26447981282147026 2202 56-57 0.43997301590204785 2202 58-59 0.23836825826204233 2202 60-61 0.16665039518196068 2202 62-63 0.29835298250689846 2202 64-65 0.25095922796422343 2202 66-67 0.30314010805593483 2202 68-69 0.31518626011256856 2202 70-71 0.2185565242518237 2202 72-73 0.3939265121665372 2202 74-75 0.49499518128314435 2202 76-77 0.42272953935953694 2202 78-79 0.49943007000301165 2202 80-81 0.36051745038005123 2202 82-83 0.30741397974410134 2202 84-85 0.3904071962479243 2202 86-87 0.3838559973292135 2202 88-89 0.3556379257705906 2202 90-91 0.3311830059237799 2202 92-93 0.32411286088025903 2202 94-95 0.3597713739433175 2202 96-97 0.4825215625277828 2202 98-99 0.49247861275617666 2202 100-101 0.3135981849727436 1102 1 0.02461142895738888 1102 2 -0.1587414419251978 1102 3 -0.1775322234058585 1102 4 -0.30861188760950853 1102 5 -0.21674521014925574 1102 6 0.08035732587609346 1102 7 -0.14543618924079738 1102 8 0.20775626686100424 1102 9 -0.2492548772050256 1102 10-11 -0.029314491132730325 1102 12-13 -0.12353979745571309 1102 14-15 -0.18509690764059883 1102 16-17 -0.03752192703324653 1102 18-19 0.05274891222694578 1102 20-21 -0.16088399943014053 1102 22-23 0.20013966263527294 1102 24-25 0.033400278570873354 1102 26-27 0.2140730593341118 1102 28-29 0.11046622707990394 1102 30-31 0.106319886576145 1102 32-33 0.11491612465312784 1102 34-35 0.0038540342840391872 1102 36-37 -0.018033311786183504 1102 38-39 -0.007085178860641861 1102 40-41 0.04188387898744139 1102 42-43 0.07906322943963318 1102 44-45 0.13202665870715435 1102 46-47 0.14031202991510838 1102 48-49 0.12814133504338798 1102 50-51 0.07116655812606609 1102 52-53 0.2017481260200924 1102 54-55 0.38977608903827843 1102 56-57 0.3491991429296277 1102 58-59 0.08048257379984136 1102 60-61 0.10138738775451372 1102 62-63 0.3392934812670134 1102 64-65 0.18771037126268908 1102 66-67 0.1824951462222586 1102 68-69 0.2853678538202544 1102 70-71 0.2553278733750872 1102 72-73 0.5765735821701838 1102 74-75 0.6109462635305434 1102 76-77 0.5162645137199604 1102 78-79 0.46108901157912996 1102 80-81 0.4406511788302403 1102 82-83 0.31618609720903024 1102 84-85 0.39830090956253983 1102 86-87 0.29612593385141395 1102 88-89 0.27692561494545487 1102 90-91 0.2538686259817169 1102 92-93 0.35349629271096816 1102 94-95 0.2913422091622131 1102 96-97 0.38346756513488245 1102 98-99 0.34242694915049654 1102 100-101 0.31181810673081856 2305 1 0.1604524975787065 2305 2 0.03634206326429279 2305 3 0.044783043548736856 2305 4 -0.10328753376049349 2305 5 0.03692530989874854 2305 6 0.3141224654176611 2305 7 -0.2591041155990226 2305 8 0.4177764670029944 2305 9 0.058096701406491036 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88-89 -0.33825640108102206 1213 90-91 -0.37391816790882615 1213 92-93 -0.3496674147058805 1213 94-95 -0.2884552930865212 1213 96-97 -0.3802956298628537 1213 98-99 -0.36638201877253707 1213 100-101 -0.3011831808093177 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1033.0 3 358.0 4 483.0 5 399.0 6 1201.0 7 4095.0 8 17451.0 9 57140.0 10 83359.0 11 64440.0 12 38435.0 13 25511.0 14 18274.0 15 14084.0 16 12151.0 17 11522.0 18 11196.0 19 11220.0 20 11738.0 21 12396.0 22 12954.0 23 13667.0 24 14550.0 25 16236.0 26 18929.0 27 22892.0 28 28471.0 29 35081.0 30 41108.0 31 51570.0 32 70583.0 33 100944.0 34 154601.0 35 259307.0 36 502061.0 37 832144.0 38 266386.0 39 390.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.43525057912876 21.900742979829367 23.43705859088084 13.226947850161025 2 30.11554913380522 27.61533903805099 30.8718938490319 11.397217979111891 3 35.82331404258745 19.284238367390742 30.596949324988643 14.29549826503317 4 26.891468821073595 17.444335133261394 35.014619341160675 20.649576704504337 5 35.88702218720392 15.899052670075303 31.72513876710143 16.488786375619352 6 31.75485951980494 16.253675682082424 37.26894704760185 14.722517750510786 7 35.59162378260036 19.894503786211644 28.514412713997135 15.999459717190867 8 26.753867046867942 33.71210050742482 24.170722409458577 15.36331003624866 9 34.13410713139686 27.13533214710626 22.42053794904019 16.31002277245668 10-11 27.137013240671664 30.87571551664568 26.46339812410891 15.523873118573746 12-13 29.519345246491785 23.586840605551075 26.471276949900407 20.422537198056727 14-15 25.124684629529188 24.051455200641566 29.40273752501275 21.4211226448165 16-17 27.547073870308214 25.048662452545294 30.419004874003036 16.985258803143452 18-19 27.37124542780028 25.19328546234863 30.910617693670243 16.524851416180848 20-21 25.441856086449256 29.203402063564095 28.807162216813502 16.54757963317315 22-23 29.86700603552434 25.701192143091045 27.797658199618336 16.63414362176628 24-25 23.891361727548134 31.624362512829514 27.725307509452623 16.75896825016973 26-27 22.706226296247124 33.055448550624845 27.582527908840405 16.655797244287633 28-29 24.62206282699229 30.870648794352977 27.490082800104055 17.01720557855068 30-31 27.984701432667997 26.731090429348033 28.18660661463005 17.097601523353923 32-33 27.361204779022785 26.524036094579888 29.23560207573003 16.879157050667292 34-35 22.399947260432192 26.551314768793375 28.096085695435 22.952652275339428 36-37 18.948217436178805 26.783611135219466 29.55813600375725 24.71003542484448 38-39 19.12354590910015 29.293542294036385 31.617153256606773 19.965758540256694 40-41 21.907491868194032 26.529521991231793 33.899218639513506 17.663767501060672 42-43 23.506959087127612 26.150731448991298 32.944193805912946 17.398115657968138 44-45 20.884875217610812 29.28893059222668 32.50295922422364 17.32323496593887 46-47 24.038716905306927 30.706510495774562 27.93783199900133 17.316940599917178 48-49 19.890719135342476 29.063084364537573 31.313762419666315 19.732434080453643 50-51 19.061277719176466 26.83495281582737 32.860442559144204 21.243326905851955 52-53 26.596344842132137 26.278892438335365 27.740117579024098 19.384645140508404 54-55 27.87485719730026 26.51331973787408 27.942964120042824 17.66885894478284 56-57 28.653171029299116 26.298408408583597 27.397299988633133 17.651120573484153 58-59 26.039079698276336 26.28969120236605 27.552576624935792 20.118652474421825 60-61 20.714517976050782 26.46464521510469 27.224686497108845 25.596150311735684 62-63 24.757569589190005 26.56643264710684 27.762328434542727 20.91366932916042 64-65 20.613557074682227 28.955601591124985 31.085424017279596 19.345417316913192 66-67 19.004013909513937 28.92446558522794 28.80827021229557 23.263250292962553 68-69 18.660181303414518 26.955792426198922 35.74351382678432 18.64051244360224 70-71 18.209597434709522 32.87214858273285 30.978907525221956 17.939346457335684 72-73 18.130445797934946 36.8554749405399 26.842980457228833 18.17109880429632 74-75 18.528155875702712 37.021044795924304 26.312558870284214 18.138240458088774 76-77 18.78280038179282 36.54652066377672 26.546686752842152 18.123992201588308 78-79 19.06367061051977 36.074275900089056 26.736479227626763 18.125574261764417 80-81 18.55250445901024 36.13670610576474 26.690602387410543 18.62018704781448 82-83 18.38680539351901 35.38065036166557 26.873431191622654 19.359113053192768 84-85 18.634032175081536 34.2131053462357 27.458310658872836 19.694551819809927 86-87 18.796468311135072 33.791686920554675 27.801302730076806 19.610542038233447 88-89 19.26965618838632 34.05974942489481 27.23702394070875 19.433570446010112 90-91 19.775496429966964 33.35609614886033 26.87678795465322 19.991619466519488 92-93 19.523788489434896 33.34450595176044 27.196355971284547 19.935349587520122 94-95 19.128869630638192 33.294491332638714 27.317564364493336 20.259074672229758 96-97 19.523612455054945 33.8728094697208 26.783471387484862 19.82010668773939 98-99 19.96349478650972 33.0603189707983 26.7216607380013 20.254525504690672 100-101 20.18452407992049 32.89800941162247 26.239453551081905 20.678012957375138 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 715.0 1 502.5 2 266.5 3 278.0 4 301.5 5 359.5 6 485.0 7 640.0 8 845.5 9 1038.5 10 1196.5 11 1283.5 12 1233.5 13 1103.0 14 1014.0 15 994.5 16 945.5 17 887.5 18 904.0 19 998.0 20 1162.5 21 1429.5 22 1857.0 23 2535.5 24 3531.0 25 5020.0 26 7740.0 27 11625.5 28 15813.0 29 19883.5 30 24399.5 31 29298.0 32 36120.5 33 47116.5 34 62918.0 35 85602.5 36 110901.5 37 139022.0 38 170806.5 39 186656.0 40 182629.0 41 168381.5 42 155530.0 43 154170.5 44 148644.5 45 140786.5 46 142887.5 47 134945.5 48 112163.5 49 93151.5 50 80225.5 51 68893.5 52 59279.0 53 50102.5 54 41029.0 55 32767.0 56 24786.5 57 18216.5 58 13422.5 59 9917.5 60 7373.0 61 5421.0 62 3809.5 63 2706.5 64 1966.0 65 1426.0 66 1066.0 67 784.0 68 571.0 69 428.0 70 338.5 71 261.5 72 208.5 73 165.5 74 128.5 75 108.0 76 95.0 77 75.5 78 63.5 79 59.0 80 46.5 81 43.0 82 37.0 83 25.0 84 26.0 85 24.5 86 15.5 87 16.5 88 15.0 89 7.5 90 6.5 91 7.5 92 6.5 93 6.0 94 5.5 95 4.0 96 4.5 97 4.5 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22971011429135132 2 0.22259332854183403 3 0.20226468805930187 4 0.19268168942628844 5 0.19574683972434787 6 0.12373342352626163 7 0.09893036824081512 8 0.09403317408644428 9 0.08730393607576206 10-11 0.06863118138643443 12-13 0.09611183923110528 14-15 0.09885990501557237 16-17 0.08800856832818953 18-19 0.08224819966459504 20-21 0.07747431615439901 22-23 0.15755577164277965 24-25 0.624128017587621 26-27 0.3554693555433419 28-29 0.18147803661269182 30-31 0.3105314336447808 32-33 0.3508187826773207 34-35 0.3299264363928466 36-37 0.341588100170521 38-39 0.3494095181724658 40-41 0.35090686170887414 42-43 0.3728385405656788 44-45 0.3493566707535337 46-47 0.37356078862441694 48-49 0.360577939373441 50-51 0.36637353964965685 52-53 0.3600494651841204 54-55 0.34243365887343397 56-57 0.3510654039656703 58-59 0.34100677856226835 60-61 0.3184937780972111 62-63 0.30588086077875953 64-65 0.3052466917515749 66-67 0.31967403712002707 68-69 0.3175953719753661 70-71 0.30239293112924365 72-73 0.2928099324962302 74-75 0.30001479727730096 76-77 0.30133598275060247 78-79 0.3044011330486619 80-81 0.3162565706957539 82-83 0.34456517143702703 84-85 0.3301025944559534 86-87 0.34121816823799656 88-89 0.3434025282205217 90-91 0.32358474612099947 92-93 0.30034949759720403 94-95 0.3001557237277865 96-97 0.3098268013923533 98-99 0.3329035076593526 100-101 0.3390514240617821 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2838360.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.46289442640853 #Duplication Level Percentage of deduplicated Percentage of total 1 92.26249467549718 56.70719969757162 2 3.7085667534400755 4.55878493679952 3 1.0656963976467353 1.965023555374955 4 0.7063233883848075 1.736507194047943 5 0.43387667361438004 1.3333658092220975 6 0.24561089212660295 0.9057573799652047 7 0.2054401317640351 0.8838861590692243 8 0.17056006878289748 0.8386492400771365 9 0.12020761420182867 0.6649477110843699 >10 0.9011920332801839 10.86501764369094 >50 0.0914004467250659 3.7847745501061203 >100 0.07865206824968414 9.868077862091464 >500 0.00792585509612926 3.268302767084431 >1k 0.0019954344362751518 1.9465946494809212 >5k 0.0 0.0 >10k+ 5.756675401860204E-5 0.6731108443340416 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT 19024 0.6702461985089981 Illumina Single End PCR Primer 1 (96% over 32bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3379 0.11904761904761905 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 3242 0.11422088811849096 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0048971941543708335 0.0 0.0 2 0.0053552051184486815 0.0 0.0 3 0.005813216082526529 0.0 0.0 4 0.006412153497089869 0.0 0.0 5 0.007574796713595175 0.0 0.0 6 0.008385123803886752 0.0 0.0 7 0.009829619921363041 0.0 0.0 8 0.011133189588353838 0.0 0.0 9 0.012436759255344636 0.0 0.0 10-11 0.0197649346805902 0.0 0.0 12-13 0.024996829154864078 0.0 0.0 14-15 0.031091898138361586 0.0 0.0 16-17 0.03547823390972252 0.0 0.0 18-19 0.040199270000986484 0.0 0.0 20-21 0.04479699544807565 0.0 0.0 22-23 0.05103299088205865 0.0 0.0 24-25 0.05756845502332333 0.0 0.0 26-27 0.06457954593497653 0.0 0.0 28-29 0.07292943812624192 0.0 0.0 30-31 0.13946433856170465 0.0 0.0 32-33 0.1508441494384081 0.0 0.0 34-35 0.15922927324229486 0.0 0.0 36-37 0.16779055510928848 0.0 0.0 38-39 0.17712693245395228 0.0 0.0 40-41 0.18797826914133514 0.0 0.0 42-43 0.19965754872532027 0.0 0.0 44-45 0.21249947152581067 0.0 1.7615806310686452E-5 46-47 0.22719105398892317 0.0 5.2847418932059355E-5 48-49 0.24258726870446312 0.0 7.046322524274581E-5 50-51 0.26055539114136334 0.0 7.046322524274581E-5 52-53 0.2782416606772925 0.0 7.046322524274581E-5 54-55 0.2968439521413774 0.0 8.807903155343225E-5 56-57 0.31812384616468664 0.0 1.0569483786411871E-4 58-59 0.3400907566341127 0.0 1.0569483786411871E-4 60-61 0.3639425583787821 0.0 1.0569483786411871E-4 62-63 0.3899787201059767 0.0 1.4092645048549161E-4 64-65 0.4180054679462788 0.0 1.761580631068645E-4 66-67 0.44930875576036866 0.0 2.1138967572823742E-4 68-69 0.4820741554982455 0.0 2.1138967572823742E-4 70-71 0.5182746374667061 0.0 2.1138967572823742E-4 72-73 0.5556377626516721 0.0 2.1138967572823742E-4 74-75 0.5989726461759608 0.0 2.1138967572823742E-4 76-77 0.6444566580701532 0.0 2.1138967572823742E-4 78-79 0.6927591989740554 0.0 2.1138967572823742E-4 80-81 0.74548330726194 0.0 2.1138967572823742E-4 82-83 0.8004622387575924 0.0 2.1138967572823742E-4 84-85 0.8625931876153835 0.0 2.1138967572823742E-4 86-87 0.9326512493129836 0.0 2.1138967572823742E-4 88-89 1.0072541890387408 0.0 2.1138967572823742E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 3590 0.0 87.64245 9 CGGGAGA 4340 0.0 87.4474 4 GAGAGCG 4090 0.0 84.34298 7 CGGAAAG 2030 0.0 82.48873 5 AAGCGGC 1195 0.0 82.47765 9 TCGGGAG 4605 0.0 82.212006 3 GATCGGG 5830 0.0 81.633995 1 ATCGGGA 5810 0.0 80.35335 2 GTTCGGA 555 0.0 77.79173 2 AGAGCGG 4190 0.0 77.58295 8 ATCCGGA 1030 0.0 75.08192 2 GATCCGG 1065 0.0 74.85113 1 ATTCGGA 880 0.0 73.862305 2 AAAGCGG 1565 0.0 71.45849 8 TCGGGAA 1685 0.0 70.951675 3 AAGCGTC 675 0.0 70.902 9 TCGGAGA 1110 0.0 68.809685 4 GAGCGTC 11595 0.0 68.65614 9 AGAGCGT 11735 0.0 68.32403 8 CCGGAAA 1005 0.0 67.97404 4 >>END_MODULE