FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n02_34012-1.34200000035798.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n02_34012-1.34200000035798.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2838360
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT190240.6702461985089981Illumina Single End PCR Primer 1 (96% over 32bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC33790.11904761904761905No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG32420.11422088811849096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC35900.087.642459
CGGGAGA43400.087.44744
GAGAGCG40900.084.342987
CGGAAAG20300.082.488735
AAGCGGC11950.082.477659
TCGGGAG46050.082.2120063
GATCGGG58300.081.6339951
ATCGGGA58100.080.353352
GTTCGGA5550.077.791732
AGAGCGG41900.077.582958
ATCCGGA10300.075.081922
GATCCGG10650.074.851131
ATTCGGA8800.073.8623052
AAAGCGG15650.071.458498
TCGGGAA16850.070.9516753
AAGCGTC6750.070.9029
TCGGAGA11100.068.8096854
GAGCGTC115950.068.656149
AGAGCGT117350.068.324038
CCGGAAA10050.067.974044
Produced by FastQC (version 0.11.2)