##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n02_30749-1.3420000003587d.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4883015 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.742868289366303 33.0 27.0 33.0 15.0 33.0 2 27.10337875267637 33.0 22.0 33.0 6.0 33.0 3 26.600361866592667 33.0 22.0 33.0 6.0 33.0 4 28.95292416672896 37.0 15.0 37.0 6.0 37.0 5 29.22970009307774 37.0 22.0 37.0 6.0 37.0 6 31.43210741724119 37.0 27.0 37.0 15.0 37.0 7 29.031602606176715 37.0 15.0 37.0 6.0 37.0 8 30.54397621141856 37.0 27.0 37.0 15.0 37.0 9 28.591537605352432 37.0 15.0 37.0 6.0 37.0 10-11 28.867665673769174 37.0 18.5 37.0 6.0 37.0 12-13 28.86429787743843 37.0 18.5 37.0 6.0 37.0 14-15 29.770299394943493 37.0 15.0 40.0 6.0 40.0 16-17 29.563005233446958 37.0 18.5 40.0 6.0 40.0 18-19 29.743120080524022 37.0 18.5 40.0 6.0 40.0 20-21 29.79772087531986 37.0 18.5 40.0 6.0 40.0 22-23 32.73154598132506 37.0 27.0 40.0 18.5 40.0 24-25 30.464465499286813 37.0 22.0 40.0 6.0 40.0 26-27 30.461942160734708 37.0 21.0 40.0 10.5 40.0 28-29 29.40932569324485 37.0 15.0 40.0 6.0 40.0 30-31 29.206393488449248 37.0 15.0 40.0 6.0 40.0 32-33 29.00316914856907 37.0 15.0 40.0 6.0 40.0 34-35 29.313733113660312 37.0 15.0 40.0 6.0 40.0 36-37 28.828618077151106 37.0 15.0 40.0 6.0 40.0 38-39 28.82882962677772 37.0 15.0 40.0 6.0 40.0 40-41 29.234656559523163 37.0 15.0 40.0 6.0 40.0 42-43 28.159336700788344 37.0 10.5 40.0 6.0 40.0 44-45 27.790575187665816 37.0 6.0 40.0 6.0 40.0 46-47 27.876312687960205 37.0 10.5 40.0 6.0 40.0 48-49 27.79311306641491 37.0 10.5 40.0 6.0 40.0 50-51 27.422349101938046 35.0 15.0 38.5 6.0 38.5 52-53 27.93831321427438 37.0 15.0 37.0 6.0 40.0 54-55 28.1677481023507 37.0 15.0 40.0 6.0 40.0 56-57 28.949287786336924 37.0 18.5 40.0 6.0 40.0 58-59 27.62685983966873 37.0 10.5 40.0 6.0 40.0 60-61 27.382359873971307 37.0 10.5 37.0 6.0 40.0 62-63 26.88819325764922 37.0 6.0 37.0 6.0 40.0 64-65 26.724137648563442 37.0 6.0 37.0 6.0 40.0 66-67 26.97667987913205 33.0 10.5 37.0 6.0 40.0 68-69 27.793202355511912 33.0 15.0 37.0 6.0 40.0 70-71 27.111780938620914 33.0 15.0 37.0 6.0 38.5 72-73 28.383994417383523 33.0 18.5 37.0 10.5 37.0 74-75 29.320486011204146 33.0 22.0 37.0 15.0 37.0 76-77 29.010021369993744 33.0 22.0 37.0 15.0 37.0 78-79 28.213014193075384 33.0 22.0 37.0 6.0 37.0 80-81 27.395194567290908 33.0 18.5 37.0 6.0 37.0 82-83 26.659566681650578 33.0 15.0 37.0 6.0 37.0 84-85 26.210284322288587 33.0 15.0 37.0 6.0 37.0 86-87 25.89953880952649 33.0 15.0 37.0 6.0 37.0 88-89 25.660971960970834 33.0 15.0 37.0 6.0 37.0 90-91 25.46244441190535 33.0 15.0 37.0 6.0 37.0 92-93 25.301943369004597 33.0 15.0 37.0 6.0 37.0 94-95 25.161277919482124 33.0 15.0 37.0 6.0 37.0 96-97 24.983026470326223 33.0 15.0 37.0 6.0 37.0 98-99 24.875247157749875 33.0 15.0 37.0 6.0 37.0 100-101 23.911285035986985 30.0 10.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -1.226517886653081 2306 2 -1.5886114708300028 2306 3 -1.5541282633355138 2306 4 -1.2858289244022743 2306 5 -1.5437259099386793 2306 6 -1.9544857550786006 2306 7 0.01916616869996446 2306 8 -2.1874891407676387 2306 9 -1.3032868675346094 2306 10-11 -1.3432338091060778 2306 12-13 -1.4524889619791104 2306 14-15 -0.694020290543353 2306 16-17 -0.903584300054213 2306 18-19 -0.828273194857335 2306 20-21 -1.0779292790443336 2306 22-23 -1.2319655526929623 2306 24-25 -0.40588006887810835 2306 26-27 -1.8341349863878378 2306 28-29 -1.189224461030836 2306 30-31 -0.6379856622244446 2306 32-33 -0.7879396055768204 2306 34-35 -1.1625430146955118 2306 36-37 -0.8719332999800358 2306 38-39 -0.8403126270789762 2306 40-41 -1.2223537084289013 2306 42-43 -0.46032967156445537 2306 44-45 -0.28356716482077715 2306 46-47 -0.48468510325791314 2306 48-49 -0.4682393642406133 2306 50-51 -0.6945884979596428 2306 52-53 -0.7107430298776087 2306 54-55 -0.3905368150582653 2306 56-57 -0.3751117661991401 2306 58-59 0.01253667444996509 2306 60-61 -0.2719457380367949 2306 62-63 -0.1588946796931765 2306 64-65 -0.20626646165121443 2306 66-67 -0.5439593239525671 2306 68-69 -0.8339699944042351 2306 70-71 -0.48062555613042335 2306 72-73 -1.2679276971059394 2306 74-75 -1.368537032789252 2306 76-77 -1.1103440396369813 2306 78-79 -1.0784928746596698 2306 80-81 -0.6987649643617928 2306 82-83 -0.5106716474241928 2306 84-85 -0.367582721876488 2306 86-87 -0.2297672619968978 2306 88-89 -0.19609705189190763 2306 90-91 -0.13519706612971305 2306 92-93 -0.15558513084863534 2306 94-95 -0.15637036799506987 2306 96-97 -0.07127760848883824 2306 98-99 -0.17920872330390125 2306 100-101 -0.19246623676098196 2307 1 -1.09209139704285 2307 2 -1.4603699618457995 2307 3 -1.396293399414482 2307 4 -1.19574710801567 2307 5 -1.3085804013849156 2307 6 -1.633003504325643 2307 7 -0.004839561360334699 2307 8 -1.8545805515700877 2307 9 -1.1261490148135955 2307 10-11 -1.1535149041298602 2307 12-13 -1.141850212655111 2307 14-15 -0.5564322754639868 2307 16-17 -0.8110221050721691 2307 18-19 -0.7176573338902479 2307 20-21 -1.0031797675333038 2307 22-23 -0.9658185618049373 2307 24-25 -0.3912569045134191 2307 26-27 -1.5141635379998952 2307 28-29 -0.8881406517819421 2307 30-31 -0.42749837271168545 2307 32-33 -0.6027226468226452 2307 34-35 -0.9744350723692357 2307 36-37 -0.622780961548159 2307 38-39 -0.7958883380533095 2307 40-41 -1.0856023235267322 2307 42-43 -0.5692451325831236 2307 44-45 -0.39688536071341574 2307 46-47 -0.5422771721955151 2307 48-49 -0.4045093819449299 2307 50-51 -0.4970144834946204 2307 52-53 -0.6383618275195744 2307 54-55 -0.3171212715295333 2307 56-57 -0.3779614710180965 2307 58-59 -0.04796763424526418 2307 60-61 -0.3055742097104108 2307 62-63 -0.21489875025270422 2307 64-65 -0.1271946376645765 2307 66-67 -0.45355719393094773 2307 68-69 -0.6988307108762335 2307 70-71 -0.4835442012118101 2307 72-73 -1.0672711561648676 2307 74-75 -1.1213632276976604 2307 76-77 -0.9647047223742717 2307 78-79 -0.9278559340093118 2307 80-81 -0.6911548703686456 2307 82-83 -0.44527348158628044 2307 84-85 -0.48985185953676336 2307 86-87 -0.46475534192660817 2307 88-89 -0.291683793308227 2307 90-91 -0.15374323034190596 2307 92-93 -0.14368636419135328 2307 94-95 -0.13718314949125698 2307 96-97 -0.058788887789518185 2307 98-99 -0.10066298990903633 2307 100-101 -0.051472297525773314 2201 1 -0.8517612901878238 2201 2 -1.4614920747080724 2201 3 -1.2534943955330142 2201 4 -1.1393406738138303 2201 5 -1.248894683901252 2201 6 -1.3622107306878775 2201 7 -0.02099625404781591 2201 8 -1.512176941733955 2201 9 -1.1463972210669091 2201 10-11 -0.9445067385137911 2201 12-13 -1.0447844481615824 2201 14-15 -0.6478076249181335 2201 16-17 -0.7687971944753969 2201 18-19 -0.49366939469516424 2201 20-21 -0.9553604600389569 2201 22-23 -0.8016960256737704 2201 24-25 -0.5016579550093567 2201 26-27 -1.302355450776485 2201 28-29 -0.7968999164176331 2201 30-31 -0.2799182314419397 2201 32-33 -0.5873619741210021 2201 34-35 -0.9025989644875558 2201 36-37 -0.6920041237484895 2201 38-39 -0.7397759192066538 2201 40-41 -1.0235236572654642 2201 42-43 -0.4496979573991169 2201 44-45 -0.36481740268351004 2201 46-47 -0.4870385227277758 2201 48-49 -0.44401336397465485 2201 50-51 -0.48995871535163005 2201 52-53 -0.610649765299474 2201 54-55 -0.22287669929914955 2201 56-57 -0.06689785674771542 2201 58-59 -0.010284223083917965 2201 60-61 -0.282381475697818 2201 62-63 -0.18458201903788662 2201 64-65 -0.1965813180313738 2201 66-67 -0.346095096224019 2201 68-69 -0.5817125931234237 2201 70-71 -0.2294047214594599 2201 72-73 -0.7865859408573712 2201 74-75 -0.7606424526567146 2201 76-77 -0.6536463399990673 2201 78-79 -0.6546013821532988 2201 80-81 -0.6281532463369253 2201 82-83 -0.3297757075003709 2201 84-85 -0.1676935827000392 2201 86-87 -0.29462388841606924 2201 88-89 -0.17024395860180874 2201 90-91 -0.04172410905156809 2201 92-93 0.06216177962229352 2201 94-95 -0.05611905062730571 2201 96-97 -0.036962064377558335 2201 98-99 -0.08312614900453852 2201 100-101 -0.10237928086219128 1101 1 -0.8864320860374519 1101 2 -1.6241429946657462 1101 3 -1.3215565348270495 1101 4 -1.516733784693244 1101 5 -1.553753192201608 1101 6 -1.3465472255076634 1101 7 -0.3969901455443612 1101 8 -1.4765197155991707 1101 9 -1.3947462584737416 1101 10-11 -1.2044303434610555 1101 12-13 -1.305259213763268 1101 14-15 -1.008252351876557 1101 16-17 -1.1068916204659907 1101 18-19 -0.9761322533662558 1101 20-21 -1.2542236427398024 1101 22-23 -0.8431739656817534 1101 24-25 -0.7692150575012064 1101 26-27 -1.2770169830961287 1101 28-29 -1.005202209819597 1101 30-31 -0.6631241104889192 1101 32-33 -0.9149670996825279 1101 34-35 -1.0583169655805769 1101 36-37 -0.8535728773579727 1101 38-39 -0.8759408953113947 1101 40-41 -1.1759583377512008 1101 42-43 -0.862296164382812 1101 44-45 -0.6242086453531108 1101 46-47 -0.7474249165789644 1101 48-49 -0.6441542549014336 1101 50-51 -0.5654126864602667 1101 52-53 -0.6738130863718723 1101 54-55 -0.4383062906573194 1101 56-57 -0.33434798291001755 1101 58-59 -0.23441261793510293 1101 60-61 -0.5522639567734728 1101 62-63 -0.3844287250584095 1101 64-65 -0.4331501685397079 1101 66-67 -0.5708659304455921 1101 68-69 -0.7382747769909308 1101 70-71 -0.44399613777180136 1101 72-73 -0.7883727556371589 1101 74-75 -0.8068240566039862 1101 76-77 -0.6409897999081373 1101 78-79 -0.7196232231539135 1101 80-81 -0.5236761319071341 1101 82-83 -0.6001862873043251 1101 84-85 -0.352508564037322 1101 86-87 -0.2744569123190779 1101 88-89 -0.3070734670145647 1101 90-91 -0.37483250785622246 1101 92-93 -0.24004732034243403 1101 94-95 -0.2636426648768655 1101 96-97 -0.21336583800633946 1101 98-99 -0.13940170607602553 1101 100-101 -0.2334564859893007 2304 1 -0.6649229204937193 2304 2 -1.2070902798596066 2304 3 -1.0783276449085086 2304 4 -1.0916863522782343 2304 5 -1.1917980273465112 2304 6 -0.8114473800969542 2304 7 -0.21279183483263964 2304 8 -0.8698131476089301 2304 9 -1.0426899091897965 2304 10-11 -0.7895126895341917 2304 12-13 -0.9132551357799841 2304 14-15 -0.7507204811194406 2304 16-17 -0.7389907191438354 2304 18-19 -0.6216379700526886 2304 20-21 -0.8272047363704687 2304 22-23 -0.5517642275483539 2304 24-25 -0.3247357088449725 2304 26-27 -0.7324857740548474 2304 28-29 -0.6554993443207806 2304 30-31 -0.3374124117212389 2304 32-33 -0.5102309429338092 2304 34-35 -0.6544803598002673 2304 36-37 -0.45474526212802147 2304 38-39 -0.4227085480338566 2304 40-41 -0.6903087155810255 2304 42-43 -0.3288022197203908 2304 44-45 -0.2284632515046603 2304 46-47 -0.30201944913411083 2304 48-49 -0.2777780630690181 2304 50-51 -0.26856022925571565 2304 52-53 -0.35848062021219107 2304 54-55 -0.07186166012005657 2304 56-57 -0.02919616375823253 2304 58-59 -0.019326630299406844 2304 60-61 -0.13507911134150774 2304 62-63 -0.11049211091809141 2304 64-65 -0.09346160007053683 2304 66-67 -0.2393916360514723 2304 68-69 -0.4402930103088174 2304 70-71 -0.20709031821476742 2304 72-73 -0.4920882121573271 2304 74-75 -0.41782717247793855 2304 76-77 -0.29407244637475927 2304 78-79 -0.30938235575354867 2304 80-81 -0.32863021562906525 2304 82-83 -0.12564768178190278 2304 84-85 -0.20691605913821576 2304 86-87 -0.0669927717916039 2304 88-89 -0.08745812654263929 2304 90-91 -0.0074758571869288915 2304 92-93 0.037631814070898884 2304 94-95 0.007601176702412005 2304 96-97 0.006736437474323509 2304 98-99 0.1263577580900126 2304 100-101 0.16819482801966146 2202 1 -0.21920213202092498 2202 2 -0.7737178478023843 2202 3 -0.5764065217257652 2202 4 -0.7282718754898987 2202 5 -0.6934299909808139 2202 6 -0.1625690781786311 2202 7 -0.2919107843439832 2202 8 -0.277750421751616 2202 9 -0.5159088379469488 2202 10-11 -0.38516989399875357 2202 12-13 -0.3604710812189964 2202 14-15 -0.47215023517058796 2202 16-17 -0.19925907008714105 2202 18-19 -0.17261438878058755 2202 20-21 -0.3511680566372064 2202 22-23 -0.033703026774468015 2202 24-25 -0.15301827213554375 2202 26-27 -0.05831106416660248 2202 28-29 -0.16001649062696188 2202 30-31 0.14378522183432452 2202 32-33 -0.11581576423398587 2202 34-35 -0.18283354176012878 2202 36-37 -0.11220954276202733 2202 38-39 -0.1505687984677877 2202 40-41 -0.16182300216145862 2202 42-43 -0.06107020999157342 2202 44-45 0.03869937819933256 2202 46-47 -0.08839894616750144 2202 48-49 0.11225016885248706 2202 50-51 0.12343051763873802 2202 52-53 -0.08971478736662064 2202 54-55 0.04014277521430998 2202 56-57 0.2207647763834082 2202 58-59 0.19283622093810848 2202 60-61 0.02746489415563147 2202 62-63 0.1516279105045868 2202 64-65 0.18314185569699148 2202 66-67 0.18516473334829797 2202 68-69 0.10100511396293754 2202 70-71 0.18494473538468625 2202 72-73 0.04616596374961901 2202 74-75 0.2453346006297572 2202 76-77 0.08342359517093811 2202 78-79 0.20924861370047765 2202 80-81 0.20475508091881878 2202 82-83 0.19070180328868247 2202 84-85 0.2633450084046345 2202 86-87 0.23403047990079529 2202 88-89 -0.01611403212116258 2202 90-91 0.12459232539089982 2202 92-93 0.2187731703380038 2202 94-95 0.16670072454082785 2202 96-97 0.19983652321168321 2202 98-99 0.2447454681873502 2202 100-101 0.17452796624803213 1102 1 -0.23727514383659454 1102 2 -0.5411780655880278 1102 3 -0.6395987989789589 1102 4 -0.8904365889433876 1102 5 -0.965384715695194 1102 6 -0.18638182675700676 1102 7 -0.8806760649111887 1102 8 -0.13538889655086805 1102 9 -0.818293712819468 1102 10-11 -0.5150371988647642 1102 12-13 -0.566622169496398 1102 14-15 -0.8560604913778853 1102 16-17 -0.713175003060261 1102 18-19 -0.5839324538301547 1102 20-21 -0.8088237634285598 1102 22-23 -0.312226693587597 1102 24-25 -0.536415995308019 1102 26-27 -0.2068980642673317 1102 28-29 -0.5407911574995445 1102 30-31 -0.31577916700806696 1102 32-33 -0.4228202377505994 1102 34-35 -0.571720407839468 1102 36-37 -0.6435436542614035 1102 38-39 -0.5331026060649435 1102 40-41 -0.4835111967986556 1102 42-43 -0.569878490212961 1102 44-45 -0.4096263394026174 1102 46-47 -0.49033897297178086 1102 48-49 -0.4154427388723363 1102 50-51 -0.38642452255185233 1102 52-53 -0.3241498541832435 1102 54-55 -0.2513834820849574 1102 56-57 -0.04164683123656587 1102 58-59 -0.3498959533312167 1102 60-61 -0.37788482733574114 1102 62-63 -0.19246328103342947 1102 64-65 -0.3300342566694354 1102 66-67 -0.298785562761708 1102 68-69 -0.28825922596850617 1102 70-71 -0.29798794094010717 1102 72-73 -0.06599557184074811 1102 74-75 0.04397103350536824 1102 76-77 -0.03750374164022219 1102 78-79 -0.1320139571814103 1102 80-81 0.07054908409175553 1102 82-83 -0.065732724581121 1102 84-85 -0.10044345238739538 1102 86-87 -0.10208386477399145 1102 88-89 -0.1824192608317361 1102 90-91 -0.20903443259183874 1102 92-93 -0.11473195522687263 1102 94-95 -0.17043355588952736 1102 96-97 -0.06783529804278032 1102 98-99 -0.10075529913173753 1102 100-101 -0.05460584442144878 2305 1 0.4227884094118437 2305 2 0.20542427535285057 2305 3 0.14323811312487678 2305 4 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-0.2781036515428177 1213 60-61 0.251203799335169 1213 62-63 0.10855864114869362 1213 64-65 0.1917420970128454 1213 66-67 0.4701349568633191 1213 68-69 0.9762540133969857 1213 70-71 0.4065010266975513 1213 72-73 1.3899914810985727 1213 74-75 1.3622596576339632 1213 76-77 1.1167376462282057 1213 78-79 1.114137023406517 1213 80-81 0.6915612553753441 1213 82-83 0.41999484659359254 1213 84-85 0.20589561588777627 1213 86-87 0.03661387378517844 1213 88-89 0.149641396767084 1213 90-91 0.21111121875547312 1213 92-93 0.2302972369961509 1213 94-95 0.3004414351475653 1213 96-97 0.21834198874409338 1213 98-99 0.1844946588151224 1213 100-101 0.4021863271657331 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1633.0 3 997.0 4 1433.0 5 1127.0 6 2311.0 7 19650.0 8 147958.0 9 292534.0 10 295644.0 11 224228.0 12 149630.0 13 102545.0 14 71118.0 15 50736.0 16 41208.0 17 35192.0 18 31928.0 19 29479.0 20 27453.0 21 24641.0 22 22186.0 23 21258.0 24 22236.0 25 25793.0 26 30927.0 27 36828.0 28 41773.0 29 49142.0 30 59744.0 31 77166.0 32 105376.0 33 150995.0 34 230244.0 35 387024.0 36 720987.0 37 1036752.0 38 312521.0 39 618.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.29993643409866 19.021947169080168 15.122547545631676 12.555568851189495 2 28.151188499835918 39.26165426403395 21.246685727432418 11.340471508697714 3 27.0374609563684 22.24182982127035 37.66925062902774 13.051458593333518 4 21.609862338027387 17.516257531194253 29.82226740625311 31.051612724525253 5 43.845591961770396 15.53879898475699 21.645645719585286 18.96996333388733 6 47.60277413166915 15.310006712667093 23.258448344653015 13.828770811010743 7 41.216558039312474 24.48992016319931 19.391479100337566 14.902042697150652 8 22.756294432235887 44.332894617902504 18.55143147563551 14.3593794742261 9 41.39227518549992 25.27412503192267 18.642982198321043 14.690617584256374 10-11 32.51719619710246 33.643581432082655 20.737888274617827 13.101334096197062 12-13 37.44444206442512 20.17977162814947 20.47718408138764 21.89860222603777 14-15 28.15090523091316 19.90127455709044 25.79551946416086 26.152300747835543 16-17 37.75407565851199 19.756840400944018 26.321766295127286 16.16731764541671 18-19 38.17039578452893 19.86885064467932 27.307482625118617 14.653270945673135 20-21 33.614734353474795 28.78648922604865 22.773777557898896 14.824998862577656 22-23 44.98248204749562 20.193951140474343 20.173995082769956 14.649571729260083 24-25 29.384078922656578 35.37886577785031 20.333005824789417 14.9040494747037 26-27 24.700920611809458 39.96426264999951 20.45083725377269 14.883979484418342 28-29 30.645930604572268 34.17242609505729 20.260742140018888 14.920901160351555 30-31 40.54680028198732 21.731972777377912 22.849908304822378 14.871318635812388 32-33 26.873463622463582 21.060025755353728 36.97116916853606 15.095341453646629 34-35 17.800751588133895 30.64917364442997 26.06047569490777 25.489599072528367 36-37 29.18350496696685 34.09172931728335 20.689173391970357 16.03559232377944 38-39 34.14417490136699 23.48311770423668 20.433022582364895 21.939684812031434 40-41 27.485631986099364 21.458854023362395 20.383366906131855 30.672147084406394 42-43 36.7382920280048 20.932671132718607 25.362211610296377 16.966825228980216 44-45 28.648296171731374 28.102127162931396 26.78775034586971 16.46182631946752 46-47 32.04369978915272 30.866148868990035 21.19123061110361 15.898920730753636 48-49 20.660052930575702 29.275949303617793 29.061089019315027 21.002908746491478 50-51 17.290064244348216 23.287494717034992 34.29695231618647 25.125488722430322 52-53 37.33205758853408 21.573126090563495 21.84299913463896 19.251817186263466 54-55 39.83722648276491 21.180990997127257 23.075546443750717 15.906236076357116 56-57 41.75445200884165 20.830775888516435 21.860065661004363 15.554706441637551 58-59 36.577155249064845 21.260190193313306 21.563539559467436 20.599114998154405 60-61 25.432733950057635 21.198342697659207 20.544680123783092 32.82424322850006 62-63 35.04780447166449 21.10923504321386 21.858510841181445 21.984449643940206 64-65 25.793513659289086 25.324954276306567 29.619977544066916 19.26155452033743 66-67 19.12241285878565 27.056442683654826 25.044268529820556 28.77687592773897 68-69 17.221808530852417 23.035376261145274 41.59225528671893 18.150559921283378 70-71 16.52046606461143 34.55687035341802 31.770014872348582 17.152648709621964 72-73 16.002118634495297 45.88083112248607 21.427371459717808 16.689678783300824 74-75 15.725037536901487 47.285928752953325 20.641963426668024 16.347070283477162 76-77 15.79532405285996 47.23322370306045 20.383169202855168 16.588283041224418 78-79 15.798127342862545 46.38336119701514 20.744936448930794 17.073575011191515 80-81 15.949626433951225 45.166678871854465 21.0588378723451 17.82485682184921 82-83 16.331192750751182 43.8691453960249 21.174846868741746 18.62481498448217 84-85 16.634180780949393 42.86545748293502 21.291757973833995 19.20860376228159 86-87 17.07463554425272 41.530485559174586 21.486608585237263 19.908270311335432 88-89 17.778576825814778 40.57353727550531 21.53469275375868 20.113193144921233 90-91 18.322034981169185 39.53717260235025 21.62715992521497 20.513632491265597 92-93 18.62183176292455 38.96021726932079 21.596766428496903 20.821184539257757 94-95 19.035634073728716 38.27019827287861 21.783993780802028 20.91017387259065 96-97 19.378555124488734 37.86963409530314 21.801963165610967 20.94984761459716 98-99 19.769013057847097 37.24119235290661 21.87830385637724 21.111490732869047 100-101 20.120129930501214 37.82023801415371 21.432892749396018 20.62673930594906 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 931.0 1 677.0 2 348.0 3 271.5 4 290.5 5 363.0 6 484.5 7 673.0 8 897.0 9 1155.5 10 1427.0 11 1578.0 12 1641.0 13 1635.5 14 1618.5 15 1749.0 16 1730.0 17 1549.0 18 1555.0 19 1660.5 20 1942.0 21 2470.5 22 3282.5 23 4419.0 24 5943.0 25 8006.5 26 10724.0 27 14106.5 28 18051.5 29 22530.0 30 27937.0 31 34652.0 32 43039.5 33 54016.5 34 68107.5 35 84676.5 36 105144.0 37 128345.5 38 148254.5 39 162318.0 40 173908.5 41 183535.0 42 192809.5 43 202878.5 44 211145.0 45 220695.5 46 231276.5 47 239120.0 48 241098.5 49 235314.5 50 225294.5 51 215857.5 52 204329.0 53 189121.0 54 170568.0 55 148895.5 56 126911.5 57 104068.5 58 82936.0 59 66164.5 60 52525.0 61 42059.0 62 33556.0 63 26256.5 64 20551.5 65 15901.5 66 12322.5 67 9689.5 68 7687.0 69 6057.5 70 4740.0 71 3661.5 72 2836.5 73 2213.0 74 1696.5 75 1285.0 76 976.0 77 740.5 78 581.0 79 468.0 80 345.0 81 249.5 82 193.5 83 157.0 84 130.0 85 107.0 86 86.0 87 64.5 88 49.5 89 39.0 90 32.5 91 26.0 92 19.5 93 15.0 94 12.0 95 11.0 96 5.5 97 2.0 98 2.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22334561741055475 2 0.21560449845024027 3 0.19702990877562324 4 0.18676985428060328 5 0.19055849715800588 6 0.11609630525402852 7 0.09344636459236763 8 0.08965772171496503 9 0.08267433133013108 10-11 0.06465267872410796 12-13 0.0908557520302518 14-15 0.09289342752377373 16-17 0.08354469523439924 18-19 0.07874233439790784 20-21 0.07292625560232767 22-23 0.14954899790395892 24-25 0.6083638899327567 26-27 0.3472751978029967 28-29 0.1749738634839336 30-31 0.3012687857809161 32-33 0.3369639454312551 34-35 0.3192392405102175 36-37 0.3278814421008332 38-39 0.3351105822939311 40-41 0.3374144867464057 42-43 0.3567468049965032 44-45 0.3337691979238237 46-47 0.3565010551882392 48-49 0.3446538665148479 50-51 0.3510024032283333 52-53 0.34482793929570155 54-55 0.3290692328407756 56-57 0.3379879029656882 58-59 0.3280657544570312 60-61 0.3068493543435767 62-63 0.2945004264783131 64-65 0.2942546766700492 66-67 0.30712582287787366 68-69 0.3055489282748466 70-71 0.29178693901206526 72-73 0.2834519246817796 74-75 0.2895649511623454 76-77 0.2913363976969147 78-79 0.2943161141221151 80-81 0.30445329371300317 82-83 0.33162912667685845 84-85 0.31776474166063384 86-87 0.3276152131418806 88-89 0.3299703154710768 90-91 0.31120117386491747 92-93 0.2911930436420941 94-95 0.29121352279278273 96-97 0.2990467979311962 98-99 0.3194133132910712 100-101 0.3266731722102021 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4883015.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.81060452651597 #Duplication Level Percentage of deduplicated Percentage of total 1 94.3645816062249 75.31294303884557 2 3.0589357400532697 4.882710212428339 3 0.8922871472848315 2.136419299081284 4 0.4234173062473241 1.3517276471435153 5 0.3081946353059956 1.2298600077800317 6 0.17992890524712404 0.8616140819740308 7 0.12348184349090692 0.6898612403940538 8 0.08303294784379142 0.5301527810425332 9 0.06699661441661472 0.48123362680379245 >10 0.4419161364018672 6.490643169754957 >50 0.03886351078109704 2.1711648351757313 >100 0.017990988201830163 2.406488799134471 >500 2.4019866824492124E-4 0.11783130265824013 >1k 8.158459038536285E-5 0.13794282715619333 >5k 2.5417623213143337E-5 0.19014029129515606 >10k+ 2.5417618679129107E-5 1.009266839332113 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT 49752 1.0188787050623438 Illumina Single End PCR Primer 1 (96% over 32bp) GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTTCAGAGCCGTGTAGATCT 9373 0.1919510794048349 Illumina Single End PCR Primer 1 (96% over 32bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0073724942479185505 0.0 0.0 2 0.007782077261691803 0.0 0.0 3 0.008744597344058948 0.0 0.0 4 0.009256576111275514 0.0 0.0 5 0.010690116659481897 0.0 0.0 6 0.011447845234962416 0.0 0.0 7 0.013065698139366764 0.0 2.047915068866264E-5 8 0.014601634441016462 0.0 2.047915068866264E-5 9 0.015543675372694944 0.0 4.095830137732528E-5 10-11 0.025097199168956065 0.0 4.095830137732528E-5 12-13 0.03243897469084162 0.0 4.095830137732528E-5 14-15 0.038889907157770354 0.0 4.095830137732528E-5 16-17 0.04176722782952745 0.0 4.095830137732528E-5 18-19 0.04496197533695882 0.0 6.143745206598792E-5 20-21 0.048443430954031474 0.0 6.143745206598792E-5 22-23 0.05213991765333508 0.0 6.143745206598792E-5 24-25 0.056286945667789266 0.0 6.143745206598792E-5 26-27 0.06001415109312587 0.0 6.143745206598792E-5 28-29 0.06498034513512656 0.0 6.143745206598792E-5 30-31 0.1134135365138137 0.0 6.143745206598792E-5 32-33 0.12236292536475928 0.0 6.143745206598792E-5 34-35 0.12882409740703232 0.0 6.143745206598792E-5 36-37 0.1353774256274044 0.0 6.143745206598792E-5 38-39 0.14195123299846507 0.0 6.143745206598792E-5 40-41 0.14962067493136924 1.023957534433132E-5 7.167702741031924E-5 42-43 0.15834479312473954 2.047915068866264E-5 8.191660275465056E-5 44-45 0.16832837908546255 2.047915068866264E-5 8.191660275465056E-5 46-47 0.17868058975858153 2.047915068866264E-5 8.191660275465056E-5 48-49 0.18981100815786967 2.047915068866264E-5 8.191660275465056E-5 50-51 0.201688915557294 2.047915068866264E-5 8.191660275465056E-5 52-53 0.21438598898426484 2.047915068866264E-5 8.191660275465056E-5 54-55 0.22869067574029572 2.047915068866264E-5 1.023957534433132E-4 56-57 0.24445962177056593 2.047915068866264E-5 1.023957534433132E-4 58-59 0.26207169136281583 2.047915068866264E-5 1.023957534433132E-4 60-61 0.2816292802704886 2.047915068866264E-5 1.023957534433132E-4 62-63 0.3018422020001986 2.047915068866264E-5 1.2287490413197583E-4 64-65 0.3231712374424408 2.047915068866264E-5 1.2287490413197583E-4 66-67 0.34658914625492654 2.047915068866264E-5 1.2287490413197583E-4 68-69 0.3708262210949588 4.095830137732528E-5 1.2287490413197583E-4 70-71 0.39779726255192743 4.095830137732528E-5 1.2287490413197583E-4 72-73 0.42819856174924714 4.095830137732528E-5 1.2287490413197583E-4 74-75 0.45981837041254225 4.095830137732528E-5 1.2287490413197583E-4 76-77 0.4941823852681182 4.095830137732528E-5 1.4335405482063848E-4 78-79 0.5321507306448987 4.095830137732528E-5 1.6383320550930112E-4 80-81 0.5727916051865497 4.095830137732528E-5 1.6383320550930112E-4 82-83 0.6164736336054671 4.095830137732528E-5 1.6383320550930112E-4 84-85 0.6653778454499935 4.095830137732528E-5 1.6383320550930112E-4 86-87 0.7176303984321162 4.095830137732528E-5 1.6383320550930112E-4 88-89 0.7748389058808953 4.095830137732528E-5 1.6383320550930112E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 17755 0.0 90.7901 9 ATCGGGA 28170 0.0 89.12534 2 GAGAGCG 20785 0.0 87.926544 7 TCGGGAG 25255 0.0 87.403175 3 AGAGCGG 19620 0.0 84.40508 8 GATCGGG 30300 0.0 83.869934 1 GAGCGTC 41280 0.0 83.41426 9 TCCGGAG 3620 0.0 82.69177 3 CGGGAGA 25880 0.0 81.697914 4 GGAGAGC 22710 0.0 81.137764 6 AGAGCGT 42935 0.0 80.7499 8 CGGAAGA 43565 0.0 79.83941 4 AAGAGCG 42150 0.0 79.623276 7 ATTGGGA 11060 0.0 79.48562 2 TCGGAAG 44945 0.0 79.289505 3 GAAGAGC 42900 0.0 78.39172 6 CGGAGAG 8530 0.0 78.3028 5 GATCGGA 48435 0.0 77.26111 1 ATCCGGA 4915 0.0 77.22088 2 ATCGGAA 48255 0.0 77.01124 2 >>END_MODULE