FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n02_30749-1.3420000003587d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n02_30749-1.3420000003587d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4883015
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT497521.0188787050623438Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTTCAGAGCCGTGTAGATCT93730.1919510794048349Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC177550.090.79019
ATCGGGA281700.089.125342
GAGAGCG207850.087.9265447
TCGGGAG252550.087.4031753
AGAGCGG196200.084.405088
GATCGGG303000.083.8699341
GAGCGTC412800.083.414269
TCCGGAG36200.082.691773
CGGGAGA258800.081.6979144
GGAGAGC227100.081.1377646
AGAGCGT429350.080.74998
CGGAAGA435650.079.839414
AAGAGCG421500.079.6232767
ATTGGGA110600.079.485622
TCGGAAG449450.079.2895053
GAAGAGC429000.078.391726
CGGAGAG85300.078.30285
GATCGGA484350.077.261111
ATCCGGA49150.077.220882
ATCGGAA482550.077.011242
Produced by FastQC (version 0.11.2)