FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n02_30521-1.342000000356dc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n02_30521-1.342000000356dc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3070365
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT47540.15483501147257736Illumina Single End PCR Primer 1 (96% over 32bp)
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA45950.14965647406741542No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC39960.13014739289954125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC11400.064.8324369
AGAGCGG12350.058.6944588
GATCGGG25600.050.5872151
ATCGGGA24800.048.0030982
CGGGAGA22000.045.702424
TCGGGAG23050.045.6788443
TTGTGTT262600.043.9687351
GGATCGG6550.043.453831
GAGAGCG21600.041.234677
GGCGCCG14600.038.0646856-57
GGTCCGG2650.037.5916631
GAATCGG4150.036.5780031
CGCCGGA9750.036.0473858-59
GTCGCCG15400.035.77885456-57
GCCGTAT15250.035.1929158-59
GCCGGAT11800.035.0173958-59
AAGGGGC15450.034.958199
GATCTCG24250.034.6813746-47
TCGGAAA8650.034.5422334
ATCTCGG26300.033.42340546-47
Produced by FastQC (version 0.11.2)