##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n02_30521-1.342000000356dc.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3070365 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.83232579839856 33.0 33.0 33.0 27.0 33.0 2 31.696799891869535 33.0 33.0 33.0 27.0 33.0 3 31.645014517817913 33.0 33.0 33.0 27.0 33.0 4 35.2867593917987 37.0 37.0 37.0 33.0 37.0 5 35.30893916521325 37.0 37.0 37.0 33.0 37.0 6 35.4152757082627 37.0 37.0 37.0 33.0 37.0 7 35.1998052348825 37.0 37.0 37.0 33.0 37.0 8 35.283117805212086 37.0 37.0 37.0 33.0 37.0 9 35.09932760437277 37.0 37.0 37.0 33.0 37.0 10-11 35.02049968000547 37.0 37.0 37.0 33.0 37.0 12-13 35.03015130122966 37.0 37.0 37.0 33.0 37.0 14-15 36.71052269029904 40.0 37.0 40.0 33.0 40.0 16-17 36.6331237491308 40.0 37.0 40.0 33.0 40.0 18-19 36.61235423150016 40.0 37.0 40.0 33.0 40.0 20-21 36.601653549333705 40.0 37.0 40.0 33.0 40.0 22-23 36.76024023202453 40.0 37.0 40.0 33.0 40.0 24-25 36.35235729302542 40.0 37.0 40.0 33.0 40.0 26-27 36.289633805752736 40.0 37.0 40.0 33.0 40.0 28-29 36.209514829670084 40.0 37.0 40.0 33.0 40.0 30-31 36.1746956795039 40.0 37.0 40.0 33.0 40.0 32-33 36.075755325506904 40.0 37.0 40.0 33.0 40.0 34-35 36.027110457551466 38.5 37.0 40.0 27.0 40.0 36-37 35.92131668384704 37.0 37.0 40.0 27.0 40.0 38-39 35.78584712241053 37.0 37.0 40.0 27.0 40.0 40-41 35.689348986195455 37.0 37.0 40.0 27.0 40.0 42-43 35.607423384516174 37.0 37.0 40.0 27.0 40.0 44-45 35.46650544804933 37.0 37.0 40.0 27.0 40.0 46-47 35.32684224839718 37.0 37.0 40.0 27.0 40.0 48-49 35.18023345758566 37.0 37.0 40.0 27.0 40.0 50-51 34.028876859917304 37.0 33.0 38.5 24.5 38.5 52-53 34.4946631752251 37.0 33.0 38.5 27.0 40.0 54-55 35.17588723164836 37.0 37.0 40.0 27.0 40.0 56-57 35.25134259281877 37.0 37.0 40.0 27.0 40.0 58-59 34.94451197170369 37.0 33.0 40.0 27.0 40.0 60-61 34.68297156852687 37.0 33.0 40.0 27.0 40.0 62-63 34.35779475730084 37.0 33.0 38.5 27.0 40.0 64-65 34.11786839675413 37.0 33.0 37.0 27.0 40.0 66-67 33.91692600065464 37.0 33.0 37.0 27.0 40.0 68-69 33.80813812038634 37.0 33.0 37.0 27.0 40.0 70-71 33.44614793355187 37.0 33.0 37.0 27.0 38.5 72-73 33.295727869487834 37.0 33.0 37.0 27.0 37.0 74-75 33.17364678140873 37.0 33.0 37.0 27.0 37.0 76-77 32.9152797794399 37.0 33.0 37.0 24.5 37.0 78-79 32.65800222449123 37.0 33.0 37.0 22.0 37.0 80-81 32.43156969936799 37.0 33.0 37.0 22.0 37.0 82-83 32.18596486085531 35.0 33.0 37.0 22.0 37.0 84-85 32.02912227047924 33.0 33.0 37.0 22.0 37.0 86-87 31.887529495678855 33.0 33.0 37.0 22.0 37.0 88-89 31.751150107560502 33.0 33.0 37.0 22.0 37.0 90-91 31.605697368228206 33.0 33.0 37.0 18.5 37.0 92-93 31.46295098465492 33.0 33.0 37.0 15.0 37.0 94-95 31.33524059843048 33.0 33.0 37.0 15.0 37.0 96-97 31.117695941687714 33.0 33.0 37.0 15.0 37.0 98-99 30.98395549063385 33.0 33.0 37.0 15.0 37.0 100-101 29.619076070760315 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.02322113239090484 2306 2 -0.13252903649290815 2306 3 -0.062003015126453676 2306 4 -0.003554605023879276 2306 5 -0.05441931389790966 2306 6 0.00937552116631224 2306 7 0.1797720711778723 2306 8 -0.06785784919597404 2306 9 0.013993755425666166 2306 10-11 -0.003767329635003591 2306 12-13 0.07345193865069177 2306 14-15 0.3444160578159057 2306 16-17 0.33865640739702485 2306 18-19 0.26959232529614496 2306 20-21 0.19260195590290152 2306 22-23 0.24437142925360433 2306 24-25 0.34655268832670316 2306 26-27 0.2433036261245718 2306 28-29 0.1083208171638006 2306 30-31 0.248645066150452 2306 32-33 0.23028672416150897 2306 34-35 0.2457971782368915 2306 36-37 0.28470815633108515 2306 38-39 0.2232793265474342 2306 40-41 0.3594766631719395 2306 42-43 0.21244602590556383 2306 44-45 0.2818578452671616 2306 46-47 0.3369729047829324 2306 48-49 0.20442239761826642 2306 50-51 0.10645591678965616 2306 52-53 0.3000209686207782 2306 54-55 0.30279151444895547 2306 56-57 0.273350083150099 2306 58-59 0.18755914924812345 2306 60-61 0.1575825889928879 2306 62-63 0.150269123275784 2306 64-65 0.18895281324837754 2306 66-67 0.21819843709739928 2306 68-69 0.20325119258174595 2306 70-71 0.2501912316860526 2306 72-73 0.13185310600936617 2306 74-75 0.22670536736545444 2306 76-77 0.3504658163008614 2306 78-79 0.45471365492300464 2306 80-81 0.2423271847374977 2306 82-83 0.24407927399672502 2306 84-85 0.38583306166682263 2306 86-87 0.1823607047225515 2306 88-89 0.2658718148175794 2306 90-91 0.25466577010727676 2306 92-93 0.2666970665267989 2306 94-95 0.11214602240183424 2306 96-97 0.2932203650835632 2306 98-99 0.270001977966313 2306 100-101 0.12703724012043338 2307 1 0.2529393611893589 2307 2 0.24258418787471925 2307 3 0.20442050391166333 2307 4 0.3058241796330492 2307 5 0.26781783264804204 2307 6 0.21610188798188545 2307 7 0.3775883659830299 2307 8 0.2988825054272368 2307 9 0.34442030099676657 2307 10-11 0.34730838671266184 2307 12-13 0.29800890731810625 2307 14-15 0.5615499846525509 2307 16-17 0.48845190175979525 2307 18-19 0.5109423688979646 2307 20-21 0.5549300231984873 2307 22-23 0.42060762285264275 2307 24-25 0.5378787064378301 2307 26-27 0.44116643075706463 2307 28-29 0.5550443144973229 2307 30-31 0.5282770092099369 2307 32-33 0.5891975575754103 2307 34-35 0.5044193388211013 2307 36-37 0.47070379963538755 2307 38-39 0.5421345573251628 2307 40-41 0.4589491665069829 2307 42-43 0.418345123247974 2307 44-45 0.4454207071975631 2307 46-47 0.3711697369408 2307 48-49 0.44662154370884366 2307 50-51 0.40486067557978345 2307 52-53 0.39007325961048167 2307 54-55 0.4087133805076206 2307 56-57 0.4547879468464018 2307 58-59 0.4923591468997017 2307 60-61 0.3670984446241832 2307 62-63 0.5205790842130327 2307 64-65 0.4138072796870844 2307 66-67 0.44354579762342183 2307 68-69 0.3962313826977706 2307 70-71 0.3413125148215599 2307 72-73 0.3869512527532848 2307 74-75 0.4281784170762961 2307 76-77 0.29744066851978346 2307 78-79 0.4837212929925201 2307 80-81 0.5563346604354251 2307 82-83 0.5517153620267123 2307 84-85 0.4031949875663017 2307 86-87 0.24259955424372137 2307 88-89 0.46014639085802855 2307 90-91 0.3616243374651553 2307 92-93 0.5317392345195593 2307 94-95 0.5487284509340284 2307 96-97 0.4791961018148214 2307 98-99 0.24838903550144664 2307 100-101 0.35036862438316163 2201 1 0.20943341869708476 2201 2 0.22408489023284872 2201 3 0.14668246899267245 2201 4 0.3343458565169186 2201 5 0.30143787549580736 2201 6 0.36804496507136264 2201 7 0.4406351160455486 2201 8 0.32114369257454456 2201 9 0.40375804739787924 2201 10-11 0.4160527334718367 2201 12-13 0.520887255205281 2201 14-15 0.7222210173015426 2201 16-17 0.6592090325628703 2201 18-19 0.7120729488236535 2201 20-21 0.5552284870182618 2201 22-23 0.6563723743352341 2201 24-25 0.7781185386225147 2201 26-27 0.6363337905694806 2201 28-29 0.733369494880705 2201 30-31 0.8333857646832001 2201 32-33 0.7985975216130399 2201 34-35 0.7400662060513596 2201 36-37 0.6358518748806858 2201 38-39 0.7526693492159566 2201 40-41 0.7880586268973602 2201 42-43 0.8340862023443876 2201 44-45 0.8540466783778484 2201 46-47 0.6568950628796699 2201 48-49 0.6631389187558057 2201 50-51 0.6308486257961192 2201 52-53 0.6582591659766024 2201 54-55 0.7982743461144253 2201 56-57 0.7723993423468727 2201 58-59 0.8887219144466272 2201 60-61 0.7701676489924907 2201 62-63 0.8265582383193149 2201 64-65 0.8293304179262506 2201 66-67 0.7564740663353717 2201 68-69 0.7274493675696903 2201 70-71 0.848721650970532 2201 72-73 0.7773712721265582 2201 74-75 0.6942784033942218 2201 76-77 0.7394920285219158 2201 78-79 0.8213260112286846 2201 80-81 0.9169639985673754 2201 82-83 0.8983966558942384 2201 84-85 0.7897602260080987 2201 86-87 0.6516476738790082 2201 88-89 0.7760955501607611 2201 90-91 0.6816253201182612 2201 92-93 0.7351002322673104 2201 94-95 0.7352312329000199 2201 96-97 0.7515663873403753 2201 98-99 0.569179382026558 2201 100-101 0.5093488454186961 1101 1 0.18221961346696602 1101 2 0.1840900823118936 1101 3 0.2247290824622148 1101 4 0.30619586615138417 1101 5 0.2904425018984611 1101 6 0.4156438763456691 1101 7 0.46683250055122016 1101 8 0.3204615351727682 1101 9 0.4109423854108982 1101 10-11 0.4089717103968198 1101 12-13 0.46174758518111503 1101 14-15 0.6290998714753755 1101 16-17 0.6138949768366189 1101 18-19 0.6334739799284179 1101 20-21 0.6780529098641566 1101 22-23 0.6390946160742246 1101 24-25 0.6534806055904241 1101 26-27 0.6467489276484244 1101 28-29 0.6860613214454361 1101 30-31 0.6348252964791072 1101 32-33 0.7383085309891371 1101 34-35 0.7120113211909782 1101 36-37 0.6005266853047431 1101 38-39 0.6365611718153801 1101 40-41 0.7072248335259985 1101 42-43 0.6102014925215045 1101 44-45 0.7339541379332175 1101 46-47 0.6555976626986251 1101 48-49 0.7041218016236144 1101 50-51 0.5596082517433771 1101 52-53 0.6496933198195123 1101 54-55 0.750853873833492 1101 56-57 0.695997713347559 1101 58-59 0.6520864064500174 1101 60-61 0.6425958505318974 1101 62-63 0.7969082451656746 1101 64-65 0.7733101774920996 1101 66-67 0.6191200316004881 1101 68-69 0.6801102151425198 1101 70-71 0.700937299450672 1101 72-73 0.6819785917188526 1101 74-75 0.6587507661061665 1101 76-77 0.6798441481355155 1101 78-79 0.6100399927292628 1101 80-81 0.8038569110077347 1101 82-83 0.7356398477960866 1101 84-85 0.8329210869018837 1101 86-87 0.6239578764382436 1101 88-89 0.6783912173769977 1101 90-91 0.7044669053256634 1101 92-93 0.6880733480785501 1101 94-95 0.8048473784071035 1101 96-97 0.8348252161981762 1101 98-99 0.7523401933539624 1101 100-101 0.6675628941436891 2304 1 0.1467556869637221 2304 2 0.004611225295700905 2304 3 0.08712849347485374 2304 4 0.13164898663650604 2304 5 0.03322166726220388 2304 6 0.15741368836845737 2304 7 0.27626856890225326 2304 8 0.2627720439264323 2304 9 0.2451689634655807 2304 10-11 0.291590843777918 2304 12-13 0.31894783692571593 2304 14-15 0.392317452431449 2304 16-17 0.5239408133530361 2304 18-19 0.42330484397446355 2304 20-21 0.4323001103929798 2304 22-23 0.487635209890648 2304 24-25 0.5339395249094494 2304 26-27 0.4093064706844842 2304 28-29 0.47152821663497946 2304 30-31 0.40138561820553775 2304 32-33 0.4858870725329396 2304 34-35 0.3937592441001314 2304 36-37 0.4190251633582278 2304 38-39 0.46398648956377286 2304 40-41 0.49506172994259856 2304 42-43 0.490849418201428 2304 44-45 0.6215606642931775 2304 46-47 0.5791807087290977 2304 48-49 0.6533196625861848 2304 50-51 0.4990025209913469 2304 52-53 0.4693387003457232 2304 54-55 0.674480460596321 2304 56-57 0.6746340571926837 2304 58-59 0.5699210862475113 2304 60-61 0.5028023919331943 2304 62-63 0.6643801514976886 2304 64-65 0.7303394329059856 2304 66-67 0.6392803802430009 2304 68-69 0.5180664527482577 2304 70-71 0.5389212447951834 2304 72-73 0.45646332673391754 2304 74-75 0.5510933366718405 2304 76-77 0.5655922768735877 2304 78-79 0.6775469295595755 2304 80-81 0.5332156351851154 2304 82-83 0.5590891050307718 2304 84-85 0.40516901574842024 2304 86-87 0.5557097575957961 2304 88-89 0.4262220972560371 2304 90-91 0.514406660785081 2304 92-93 0.66128194381076 2304 94-95 0.496397903468754 2304 96-97 0.5550581986987595 2304 98-99 0.5504932746524283 2304 100-101 0.5744668709958525 2202 1 0.09681475269328388 2202 2 0.160908414533683 2202 3 0.2152983696114319 2202 4 0.13041266306396437 2202 5 0.18705329881808552 2202 6 0.19462522927899073 2202 7 0.2542248027589551 2202 8 0.35823126914265657 2202 9 0.3316636940252877 2202 10-11 0.3362454674600315 2202 12-13 0.3530513703928335 2202 14-15 0.4478847945838993 2202 16-17 0.47425247402298965 2202 18-19 0.4687276240994933 2202 20-21 0.5529184612315916 2202 22-23 0.4098618316643865 2202 24-25 0.43235355952545973 2202 26-27 0.5746405590018568 2202 28-29 0.4352151995144595 2202 30-31 0.39765255644240227 2202 32-33 0.39393785356856625 2202 34-35 0.3377911503595783 2202 36-37 0.3515535625653641 2202 38-39 0.38057646312007165 2202 40-41 0.6026254911285207 2202 42-43 0.5080340139011739 2202 44-45 0.6087002471544238 2202 46-47 0.506601478072362 2202 48-49 0.6515586531096673 2202 50-51 0.5282749792941459 2202 52-53 0.46297794225631606 2202 54-55 0.4462159007429989 2202 56-57 0.5578148707952053 2202 58-59 0.5039371567472344 2202 60-61 0.402789827829352 2202 62-63 0.5753746351077709 2202 64-65 0.5633805447018361 2202 66-67 0.5049611170430097 2202 68-69 0.4684732303798995 2202 70-71 0.426575640826762 2202 72-73 0.5226651259345871 2202 74-75 0.5831900926123552 2202 76-77 0.5848766664800138 2202 78-79 0.685833724069397 2202 80-81 0.5372442225255938 2202 82-83 0.6276204492533495 2202 84-85 0.5677476661194731 2202 86-87 0.5487763284409262 2202 88-89 0.49294444684061034 2202 90-91 0.5266446493311356 2202 92-93 0.5777105664410094 2202 94-95 0.54921794742868 2202 96-97 0.6183560750895083 2202 98-99 0.6961252858359224 2202 100-101 0.6099494362996545 1102 1 -0.11998466382969042 1102 2 -0.2093825590984295 1102 3 -0.1295257109816319 1102 4 -0.1535567152330728 1102 5 -0.24214500447312304 1102 6 -0.12940146081990633 1102 7 -0.1644736845250634 1102 8 0.04920767168880502 1102 9 -0.2032736791597003 1102 10-11 -0.08984320683415348 1102 12-13 -0.1315230461150847 1102 14-15 -0.06988975370020967 1102 16-17 -0.025149654752709694 1102 18-19 -2.2227986890754892E-4 1102 20-21 -0.12603541168147814 1102 22-23 0.01419743695254283 1102 24-25 -0.07346579266327069 1102 26-27 0.066824387991538 1102 28-29 -0.005326757169243024 1102 30-31 0.022526155492258226 1102 32-33 0.013064011202523318 1102 34-35 -0.07612189783208123 1102 36-37 -0.05328686357522372 1102 38-39 -0.01770574872039532 1102 40-41 -0.004494217422056579 1102 42-43 0.006128800008781354 1102 44-45 -0.0529292124126286 1102 46-47 -0.21519627920287832 1102 48-49 -0.07179554478708638 1102 50-51 -0.026202791303255424 1102 52-53 0.018597251202230325 1102 54-55 0.07510993835221313 1102 56-57 0.17295040212487578 1102 58-59 0.1140053274371482 1102 60-61 0.0401863205831674 1102 62-63 0.140154557427266 1102 64-65 0.0716286621633273 1102 66-67 0.03614750627302499 1102 68-69 0.15731971292527192 1102 70-71 0.14836460499243032 1102 72-73 0.20400982125033096 1102 74-75 0.06907636879670065 1102 76-77 0.2732138713913912 1102 78-79 0.22057246934084418 1102 80-81 0.16141539666470095 1102 82-83 0.17492105495404786 1102 84-85 0.19685894171660578 1102 86-87 0.13531732992792556 1102 88-89 0.06563539734563761 1102 90-91 0.14747365888370112 1102 92-93 0.29275985302471597 1102 94-95 0.18226161003585872 1102 96-97 0.2149195351419877 1102 98-99 0.24790712517376434 1102 100-101 0.30975966172246316 2305 1 0.09446396326167772 2305 2 0.11502833557085523 2305 3 0.10810061289257078 2305 4 0.1759489977900941 2305 5 0.10570982925281669 2305 6 0.2457365609911335 2305 7 0.15910691965883927 2305 8 0.21380442650115583 2305 9 0.19744851962050092 2305 10-11 0.2756699463537231 2305 12-13 0.26414824656646374 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-0.32905815696269514 1213 50-51 -0.21835353105586108 1213 52-53 -0.24832231586059805 1213 54-55 -0.39019141988207195 1213 56-57 -1.1269298428423795 1213 58-59 -0.5926759422693735 1213 60-61 -0.38831992486917244 1213 62-63 -0.30794906857321536 1213 64-65 -0.23023436233339822 1213 66-67 -0.17118104696491088 1213 68-69 -0.18647197395321768 1213 70-71 -0.3060443508649584 1213 72-73 -0.2123291612866538 1213 74-75 -0.28406227418171426 1213 76-77 -0.25978621956179637 1213 78-79 -0.38570178484300754 1213 80-81 -0.3893569475021721 1213 82-83 -0.30607351817231176 1213 84-85 -0.3007323146501193 1213 86-87 -0.3860104350490339 1213 88-89 -0.18680821167691875 1213 90-91 -0.1627403842887425 1213 92-93 -0.22689664709655588 1213 94-95 -0.23360290002962358 1213 96-97 -0.3356739344190096 1213 98-99 -0.2526204033249009 1213 100-101 -0.10725768001898572 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1054.0 3 238.0 4 277.0 5 366.0 6 1175.0 7 2640.0 8 8417.0 9 20337.0 10 26516.0 11 21750.0 12 14661.0 13 10479.0 14 8386.0 15 7682.0 16 7494.0 17 7865.0 18 8444.0 19 9480.0 20 10668.0 21 11706.0 22 12877.0 23 14711.0 24 17016.0 25 19724.0 26 23466.0 27 28175.0 28 34816.0 29 43010.0 30 52861.0 31 69625.0 32 95343.0 33 137770.0 34 212417.0 35 361178.0 36 669426.0 37 899009.0 38 199266.0 39 40.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.53796736980238 24.985441108303792 19.067218990539985 16.409372531353846 2 33.60101995752135 23.331267175143957 28.607608488809287 14.460104378525404 3 39.59219904446127 19.51798814714252 23.321247682949117 17.568565125447094 4 33.41422601788058 18.39768527492092 28.982324997373187 19.205763709825312 5 36.08738727096868 16.805942250886627 27.97647717616469 19.13019330198001 6 32.05298687001952 17.346143536975312 31.513224870072996 19.087644722932172 7 36.180105088903076 18.1576646504741 24.621336346429565 21.040893914193262 8 31.55901431623103 25.729348029118178 22.494741066169173 20.21689658848162 9 31.39935968102701 23.610441984376006 24.062420343133258 20.927777991463717 10-11 25.347213395775693 28.556395290099495 28.44066835482641 17.6557229592984 12-13 26.063582259505445 25.922835652134367 28.02577089125446 19.98781119710572 14-15 25.35396007246275 26.016322049142836 28.288378804025548 20.34133907436886 16-17 25.662072995127627 26.074892247965366 28.800079535629276 19.462955221277735 18-19 25.11881638960498 26.09482038443022 28.715886633795844 20.070476592168955 20-21 24.256999210762057 27.262019011785444 28.103193798085783 20.377787979366715 22-23 25.240699676348804 26.495394445412156 27.65939054933283 20.60451532890621 24-25 23.87292692834643 27.112595797298823 28.25479766899316 20.759679605361583 26-27 23.217557982325072 27.495026607831953 28.672690573703854 20.61472483613913 28-29 23.41145057134742 27.95013991865418 27.799429427504062 20.838980082494334 30-31 23.693536314716322 27.86214825412248 27.893022141968267 20.55129328919293 32-33 23.4689743899388 27.557161857266653 28.34645720759623 20.627406545198316 34-35 22.671762023525453 27.406244970881144 28.11439392020887 21.80759908538453 36-37 21.28761113857997 27.928115760918764 28.87681530023715 21.907457800264115 38-39 21.329050028645785 28.259189283437046 28.994207766749774 21.41755292116739 40-41 22.214558181102436 27.383537214802484 29.243840210788914 21.15806439330617 42-43 23.090117994751175 27.11757780685982 28.672007018916783 21.12029717947222 44-45 22.053514017424042 27.78841463763368 28.69184053806515 21.466230806877128 46-47 22.661816531929198 28.159405902079627 28.31722705488665 20.861550511104525 48-49 21.549768690264596 28.15253891605715 28.848004303923407 21.449688089754844 50-51 21.452714104419496 27.74115680084855 27.99853236438875 22.807596730343203 52-53 23.3434900806248 27.002305083194628 27.49845769424677 22.1557471419338 54-55 23.94193043652171 26.926240517324203 27.93125546150493 21.20057358464916 56-57 24.45082432931274 27.06234299374418 27.011293747071903 21.47553892987118 58-59 23.667671159953642 27.53916682338897 26.91117742375331 21.881984592904082 60-61 22.36399611461811 27.292014285606935 26.98442155282626 23.35956804694869 62-63 23.2486770533584 27.113149690246647 27.56755043874443 22.070622817650527 64-65 22.573960756590306 27.87255284054584 28.29317662017171 21.26030978269214 66-67 22.393339243797158 27.752829565126763 27.700782197120994 22.15304899395509 68-69 22.639472042860742 26.904717641665982 29.319865403471034 21.13594491200224 70-71 22.211187753481983 28.40618532789523 28.275955003323954 21.10667191529884 72-73 22.168829679822224 29.709187941567777 26.96117421056362 21.160808168046376 74-75 22.49278716114684 29.221519739919664 26.851719974598264 21.43397312433523 76-77 22.15226479612629 28.786051635865306 26.68977891460637 22.371904653402034 78-79 21.88075425789562 28.853087430663056 27.112295019173988 22.15386329226734 80-81 21.358705942065754 28.93722850622001 27.601059682359935 22.103005869354305 82-83 20.90268249493418 28.910738787016925 27.702817855804724 22.48376086224417 84-85 20.914549378892794 29.465362406614993 27.421891682458543 22.19819653203367 86-87 20.71990564786051 29.391890787856052 27.684797449433436 22.203406114850004 88-89 21.093667406270747 29.124398421488053 27.699825864334315 22.08210830790689 90-91 21.616803484911657 29.02147290580518 26.90888244696632 22.452841162316844 92-93 21.620745775911804 29.008710497482305 26.801227910963714 22.569315815642177 94-95 21.905092003516344 29.244351682857296 26.74116087082789 22.10939544279847 96-97 21.908831522560323 29.94849379764438 26.529312437691804 21.61336224210349 98-99 22.177242464746932 29.456839610811546 26.779359503284926 21.586558421156596 100-101 22.288370454501262 29.558864546666204 26.43755576916887 21.715209229663667 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 614.0 1 442.5 2 218.5 3 176.5 4 168.5 5 182.0 6 275.0 7 394.5 8 591.5 9 788.5 10 929.5 11 1059.5 12 1116.0 13 1075.5 14 965.5 15 842.5 16 712.5 17 595.5 18 542.0 19 554.5 20 619.0 21 730.5 22 899.0 23 1179.0 24 1503.5 25 1890.5 26 2524.5 27 3343.0 28 4251.0 29 5278.5 30 6814.5 31 9163.5 32 13663.0 33 21975.5 34 35598.5 35 55069.5 36 83294.0 37 115300.5 38 135597.0 39 139634.0 40 141694.0 41 145489.5 42 154853.0 43 170491.5 44 170936.5 45 163443.0 46 162054.0 47 158988.0 48 150920.0 49 135595.0 50 121750.0 51 118828.5 52 114717.0 53 104067.0 54 90100.0 55 74106.0 56 59623.0 57 45580.0 58 32821.5 59 24091.5 60 18211.0 61 14382.0 62 11471.0 63 8925.5 64 6804.0 65 4957.5 66 3579.5 67 2707.0 68 2170.0 69 1592.5 70 1113.5 71 876.5 72 678.0 73 510.0 74 395.5 75 304.0 76 249.0 77 214.5 78 169.5 79 125.0 80 91.5 81 75.5 82 66.0 83 59.5 84 45.0 85 31.0 86 27.5 87 23.0 88 17.0 89 15.0 90 16.5 91 13.0 92 7.5 93 7.0 94 5.0 95 4.5 96 3.5 97 2.0 98 2.5 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22619460552735587 2 0.21847565354607676 3 0.199487683060483 4 0.18926088592072932 5 0.1933646325436878 6 0.11865038847172893 7 0.09337652038112733 8 0.08884937132881596 9 0.08204236304152764 10-11 0.06302182313829138 12-13 0.09104780701968658 14-15 0.09308339562234458 16-17 0.08329628562076496 18-19 0.07803632467149671 20-21 0.07215754478702044 22-23 0.1536950818550889 24-25 0.6209522320636146 26-27 0.35284078603032537 28-29 0.18071141378956573 30-31 0.3102562724627202 32-33 0.3455778058960417 34-35 0.3277297650279364 36-37 0.33657236191788276 38-39 0.34451929982265955 40-41 0.34593606949010947 42-43 0.3648914705580607 44-45 0.34238600296707394 46-47 0.364972894102167 48-49 0.35275936248621903 50-51 0.3589964059647632 52-53 0.3524825224362576 54-55 0.3358232653121046 56-57 0.34440530686091064 58-59 0.33486246749165 60-61 0.31468571326210404 62-63 0.30291186878433024 64-65 0.3034166947577894 66-67 0.3156465110825586 68-69 0.31468571326210404 70-71 0.30126711319338256 72-73 0.2922128150887598 74-75 0.2982381573526275 76-77 0.3002086071200004 78-79 0.30343297946661063 80-81 0.31385519311221954 82-83 0.3386079505205407 84-85 0.3251567810341767 86-87 0.3367352090060954 88-89 0.33854281168525563 90-91 0.32103674970239693 92-93 0.3006482942581745 94-95 0.3006808636758171 96-97 0.30848123920120246 98-99 0.32893483348070995 100-101 0.3360675359444235 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3070365.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.4796243772336 #Duplication Level Percentage of deduplicated Percentage of total 1 86.33631686786161 43.58224845603472 2 6.546328248422283 6.609123820608609 3 2.1813128110904776 3.303355540592845 4 1.2141906238501412 2.4516754645725625 5 0.6888148286470129 1.7385556907784867 6 0.5010247116149241 1.5174923547619894 7 0.2700332781877507 0.9541824916589478 8 0.28042768847513133 1.1324707503360407 9 0.1950147449914477 0.8859843967671249 >10 1.450690862923503 14.954016839769679 >50 0.1932277666969921 6.866640135820686 >100 0.1351043669376154 12.67951953484093 >500 0.005824668827379787 1.9728140453469163 >1k 0.001688531473688737 1.3519204781104248 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT 4754 0.15483501147257736 Illumina Single End PCR Primer 1 (96% over 32bp) TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 4595 0.14965647406741542 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 3996 0.13014739289954125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0013027767057011137 0.0 0.0 2 0.0013679155409861694 0.0 0.0 3 0.0015307626291988087 0.0 0.0 4 0.0015959014644838644 0.0 0.0 5 0.0019215956409091427 0.0 0.0 6 0.0021821509820493653 0.0 0.0 7 0.002475275740832116 0.0 0.0 8 0.002898678170184978 0.0 0.0 9 0.0033872194348228955 0.0 0.0 10-11 0.005325099784553302 0.0 0.0 12-13 0.007507250766602667 0.0 0.0 14-15 0.00934742286340549 0.0 0.0 16-17 0.011057317289638203 0.0 0.0 18-19 0.013223183562866305 0.0 0.0 20-21 0.015893875809553586 0.0 0.0 22-23 0.018466859803313286 0.0 0.0 24-25 0.021740086276387335 0.0 0.0 26-27 0.024899319787712537 0.0 0.0 28-29 0.029426468840023905 0.0 0.0 30-31 0.06289154546772126 0.0 0.0 32-33 0.06981254671675843 0.0 0.0 34-35 0.07613101373940884 0.0 0.0 36-37 0.08347541741779886 0.0 0.0 38-39 0.09251343081360033 0.0 0.0 40-41 0.10239824906810754 0.0 0.0 42-43 0.1136509828636009 0.0 0.0 44-45 0.12778611012045799 0.0 0.0 46-47 0.14302859757716102 0.0 0.0 48-49 0.15928073698078243 0.0 0.0 50-51 0.1782035686310911 0.0 0.0 52-53 0.19813605222831815 0.0 0.0 54-55 0.2220257200691123 0.0 0.0 56-57 0.24881406608009146 0.0 0.0 58-59 0.2761398074821723 0.0 0.0 60-61 0.30603853287801286 0.0 0.0 62-63 0.33891735998814476 0.0 0.0 64-65 0.3744505946361426 0.0 0.0 66-67 0.41524379023340874 0.0 0.0 68-69 0.46131323148876435 0.0 0.0 70-71 0.5125123560228182 0.0 0.0 72-73 0.5662681798418103 0.0 0.0 74-75 0.6284269134125747 0.0 0.0 76-77 0.6988419943557199 0.0 0.0 78-79 0.7764549165978638 0.0 0.0 80-81 0.8650925867120033 0.0 0.0 82-83 0.9656669483921293 0.0 0.0 84-85 1.0807835550496439 0.0 0.0 86-87 1.2097747336228752 0.0 0.0 88-89 1.3556043011172938 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 1140 0.0 64.832436 9 AGAGCGG 1235 0.0 58.694458 8 GATCGGG 2560 0.0 50.587215 1 ATCGGGA 2480 0.0 48.003098 2 CGGGAGA 2200 0.0 45.70242 4 TCGGGAG 2305 0.0 45.678844 3 TTGTGTT 26260 0.0 43.968735 1 GGATCGG 655 0.0 43.45383 1 GAGAGCG 2160 0.0 41.23467 7 GGCGCCG 1460 0.0 38.06468 56-57 GGTCCGG 265 0.0 37.591663 1 GAATCGG 415 0.0 36.578003 1 CGCCGGA 975 0.0 36.04738 58-59 GTCGCCG 1540 0.0 35.778854 56-57 GCCGTAT 1525 0.0 35.19291 58-59 GCCGGAT 1180 0.0 35.01739 58-59 AAGGGGC 1545 0.0 34.95819 9 GATCTCG 2425 0.0 34.68137 46-47 TCGGAAA 865 0.0 34.542233 4 ATCTCGG 2630 0.0 33.423405 46-47 >>END_MODULE