##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n02_30484-1.34200000035803.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8394700 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.66857803137694 33.0 33.0 33.0 27.0 33.0 2 31.449901604583843 33.0 33.0 33.0 27.0 33.0 3 31.365771022192575 33.0 33.0 33.0 27.0 33.0 4 34.92204116883271 37.0 37.0 37.0 33.0 37.0 5 34.95088627348208 37.0 37.0 37.0 33.0 37.0 6 35.206502436060845 37.0 37.0 37.0 33.0 37.0 7 34.84065541353473 37.0 37.0 37.0 33.0 37.0 8 35.02604393248121 37.0 37.0 37.0 33.0 37.0 9 34.73069746387602 37.0 37.0 37.0 33.0 37.0 10-11 34.66775292744231 37.0 37.0 37.0 33.0 37.0 12-13 34.66737262796765 37.0 37.0 37.0 33.0 37.0 14-15 36.27766203676129 40.0 37.0 40.0 33.0 40.0 16-17 36.20392336831573 40.0 37.0 40.0 30.0 40.0 18-19 36.196491178958155 40.0 37.0 40.0 30.0 40.0 20-21 36.18212836670756 40.0 37.0 40.0 30.0 40.0 22-23 36.53854140112213 40.0 37.0 40.0 33.0 40.0 24-25 35.990004705349804 40.0 37.0 40.0 27.0 40.0 26-27 35.94122916840388 40.0 37.0 40.0 27.0 40.0 28-29 35.799820422409375 40.0 37.0 40.0 27.0 40.0 30-31 35.74678821160971 40.0 37.0 40.0 27.0 40.0 32-33 35.5974834717143 40.0 37.0 40.0 27.0 40.0 34-35 35.729571574922275 38.5 37.0 40.0 27.0 40.0 36-37 35.453204224093774 37.0 37.0 40.0 27.0 40.0 38-39 35.367331351924435 37.0 37.0 40.0 27.0 40.0 40-41 35.577566142923516 37.0 37.0 40.0 27.0 40.0 42-43 35.30640255160995 37.0 37.0 40.0 27.0 40.0 44-45 35.00786657057429 37.0 37.0 40.0 22.0 40.0 46-47 34.87530840887703 37.0 37.0 40.0 22.0 40.0 48-49 34.732297223248004 37.0 35.0 40.0 22.0 40.0 50-51 33.694665920163914 37.0 33.0 38.5 22.0 38.5 52-53 34.15834729055237 37.0 33.0 38.5 22.0 40.0 54-55 34.822352615340634 37.0 37.0 40.0 22.0 40.0 56-57 34.989588788163964 37.0 37.0 40.0 24.5 40.0 58-59 34.62963018332996 37.0 33.0 40.0 22.0 40.0 60-61 34.377126460743085 37.0 33.0 40.0 22.0 40.0 62-63 34.02273493990256 37.0 33.0 40.0 22.0 40.0 64-65 33.78751194205868 37.0 33.0 38.5 22.0 40.0 66-67 33.606540197982056 37.0 33.0 37.0 22.0 40.0 68-69 33.551583320428364 37.0 33.0 37.0 22.0 40.0 70-71 33.152700930348914 37.0 33.0 37.0 22.0 40.0 72-73 33.11530203580831 37.0 33.0 37.0 22.0 37.0 74-75 33.02996307193824 37.0 33.0 37.0 22.0 37.0 76-77 32.74262427484008 37.0 33.0 37.0 22.0 37.0 78-79 32.3592279652638 37.0 33.0 37.0 22.0 37.0 80-81 32.01515551478909 37.0 33.0 37.0 22.0 37.0 82-83 31.68857088401015 33.0 33.0 37.0 15.0 37.0 84-85 31.469547750366303 33.0 33.0 37.0 15.0 37.0 86-87 31.29228286895303 33.0 33.0 37.0 15.0 37.0 88-89 31.128803232992247 33.0 33.0 37.0 15.0 37.0 90-91 30.958536219281214 33.0 33.0 37.0 15.0 37.0 92-93 30.807032651553957 33.0 30.0 37.0 15.0 37.0 94-95 30.67078251754083 33.0 27.0 37.0 15.0 37.0 96-97 30.457802422957343 33.0 27.0 37.0 15.0 37.0 98-99 30.338747007040155 33.0 27.0 37.0 15.0 37.0 100-101 29.046440075285595 33.0 27.0 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.17516359286529948 2306 2 0.1678203125735891 2306 3 0.14862966838300906 2306 4 0.315906446318273 2306 5 0.2760011546263357 2306 6 0.1099190069133229 2306 7 0.47714883351459747 2306 8 0.1958536869657479 2306 9 0.31999238729412127 2306 10-11 0.3387398651926077 2306 12-13 0.33774029650808757 2306 14-15 0.6929008350982002 2306 16-17 0.6570346790313053 2306 18-19 0.6268455991243442 2306 20-21 0.50660841192596 2306 22-23 0.36699262499938357 2306 24-25 0.6238091898525937 2306 26-27 0.49757102833211064 2306 28-29 0.4609550562194471 2306 30-31 0.4782358633798509 2306 32-33 0.4931161310300425 2306 34-35 0.37484354653849294 2306 36-37 0.5398760325378262 2306 38-39 0.4424023798541512 2306 40-41 0.3430089399293408 2306 42-43 0.49553839354049245 2306 44-45 0.6249252820214295 2306 46-47 0.5742536989430107 2306 48-49 0.6084135380286 2306 50-51 0.3720929340476218 2306 52-53 0.4574660122339793 2306 54-55 0.5826042620286316 2306 56-57 0.5449789216345025 2306 58-59 0.6254096305830714 2306 60-61 0.5477256258297487 2306 62-63 0.5770495661275135 2306 64-65 0.6447877522537766 2306 66-67 0.5382578227699994 2306 68-69 0.45603553946420305 2306 70-71 0.45549499311687924 2306 72-73 0.37857942234219166 2306 74-75 0.30347740507990295 2306 76-77 0.3636347774780191 2306 78-79 0.5459882287701419 2306 80-81 0.5709991631344593 2306 82-83 0.5928518286123214 2306 84-85 0.6205494121228519 2306 86-87 0.5181200888634514 2306 88-89 0.6106687516594889 2306 90-91 0.7190551962957556 2306 92-93 0.5773686236007727 2306 94-95 0.5276500002562052 2306 96-97 0.5205313285397963 2306 98-99 0.5670465754414629 2306 100-101 0.4797750979975248 2307 1 0.113787260022292 2307 2 0.12291850332177745 2307 3 0.07682456840284146 2307 4 0.20550983843034487 2307 5 0.2028767266354734 2307 6 0.12174937137076824 2307 7 0.42208465747790314 2307 8 0.20388384458003372 2307 9 0.3213916574244564 2307 10-11 0.3922094758342354 2307 12-13 0.3237081744939019 2307 14-15 0.615972363545616 2307 16-17 0.5784942038832739 2307 18-19 0.5382289103923483 2307 20-21 0.4711865996274085 2307 22-23 0.41440267328982827 2307 24-25 0.5630661042641449 2307 26-27 0.4532937236482937 2307 28-29 0.4994429098288862 2307 30-31 0.520033547171721 2307 32-33 0.47278471465129 2307 34-35 0.31941832585012264 2307 36-37 0.3965788396257892 2307 38-39 0.33390898879057573 2307 40-41 0.3410289415608929 2307 42-43 0.4693623024988085 2307 44-45 0.49496661417288834 2307 46-47 0.386446089245716 2307 48-49 0.3549935751541966 2307 50-51 0.3118709188684292 2307 52-53 0.33531100981024764 2307 54-55 0.3586084240506082 2307 56-57 0.40263776351283553 2307 58-59 0.4550486809059109 2307 60-61 0.4276800324048011 2307 62-63 0.47673041403980676 2307 64-65 0.42535301788709035 2307 66-67 0.3979433326098345 2307 68-69 0.2590937878336135 2307 70-71 0.37800972564444635 2307 72-73 0.13748062514088843 2307 74-75 0.15204176159961946 2307 76-77 0.3097285828568843 2307 78-79 0.413528114761732 2307 80-81 0.47655959355893174 2307 82-83 0.5266130210594149 2307 84-85 0.438038329509677 2307 86-87 0.37563014782752546 2307 88-89 0.4584712050586077 2307 90-91 0.4829973912874834 2307 92-93 0.4457899485102672 2307 94-95 0.4484858382015169 2307 96-97 0.4394474549157721 2307 98-99 0.36923093253605543 2307 100-101 0.436026395641786 2201 1 0.17008724830150612 2201 2 0.13325526417044742 2201 3 0.19801376048078012 2201 4 0.3014487437134292 2201 5 0.25022464089055774 2201 6 0.2618379292730708 2201 7 0.5717688836253458 2201 8 0.27002857469032193 2201 9 0.3300135288291699 2201 10-11 0.3777993970852407 2201 12-13 0.4014479659964252 2201 14-15 0.6187863733454719 2201 16-17 0.5697200533655291 2201 18-19 0.6091008214230342 2201 20-21 0.4984166115066486 2201 22-23 0.4579359495264299 2201 24-25 0.5932794231063241 2201 26-27 0.4838122908239839 2201 28-29 0.6655900912008832 2201 30-31 0.674507063911328 2201 32-33 0.6371321095590972 2201 34-35 0.4573557221718332 2201 36-37 0.5754330801512069 2201 38-39 0.578821544169621 2201 40-41 0.48635193697399615 2201 42-43 0.6983268884473048 2201 44-45 0.724749446739402 2201 46-47 0.606220845346563 2201 48-49 0.6427762134968589 2201 50-51 0.5895357122521929 2201 52-53 0.53863382480165 2201 54-55 0.6677100139974783 2201 56-57 0.7401094504739021 2201 58-59 0.8250166588154073 2201 60-61 0.6826180825619232 2201 62-63 0.78167725340559 2201 64-65 0.6628848049269962 2201 66-67 0.6655128751530484 2201 68-69 0.6371260441189861 2201 70-71 0.6407710962552855 2201 72-73 0.610389849343612 2201 74-75 0.5858001818078193 2201 76-77 0.6127267657763227 2201 78-79 0.7318159874038912 2201 80-81 0.7393925697202519 2201 82-83 0.7988833272093068 2201 84-85 0.8435671861876664 2201 86-87 0.6908796015246601 2201 88-89 0.651431780433775 2201 90-91 0.5725923732897549 2201 92-93 0.6524293095568829 2201 94-95 0.7199776617254692 2201 96-97 0.7807223931221721 2201 98-99 0.7137949367184397 2201 100-101 0.687114087818081 1101 1 0.1399360876921243 1101 2 0.07618919955721282 1101 3 0.09568859238741823 1101 4 0.18426663461757187 1101 5 0.13873922104934167 1101 6 0.19442067048115064 1101 7 0.40201855807987386 1101 8 0.2720548004362229 1101 9 0.31075484002870013 1101 10-11 0.3352102601564795 1101 12-13 0.3159450791458269 1101 14-15 0.5241887278044288 1101 16-17 0.4934508099803878 1101 18-19 0.5179933963533401 1101 20-21 0.489014572994094 1101 22-23 0.45569766669515843 1101 24-25 0.5857398228443529 1101 26-27 0.5362437068672676 1101 28-29 0.5193271996549811 1101 30-31 0.5665188855727408 1101 32-33 0.5396930307184036 1101 34-35 0.41707090964981575 1101 36-37 0.5564190304093941 1101 38-39 0.5446079490395874 1101 40-41 0.5260287720686208 1101 42-43 0.5676390577594717 1101 44-45 0.5856299060026089 1101 46-47 0.4898665597200349 1101 48-49 0.4366864561941668 1101 50-51 0.5109889121420466 1101 52-53 0.5640475445549882 1101 54-55 0.7249612583107208 1101 56-57 0.6253308378516209 1101 58-59 0.686583752997052 1101 60-61 0.5765436837651521 1101 62-63 0.6322286786200877 1101 64-65 0.6580241669127176 1101 66-67 0.5270239107880812 1101 68-69 0.5857358250737761 1101 70-71 0.6460490468162519 1101 72-73 0.49418399270648194 1101 74-75 0.41491541119259523 1101 76-77 0.5830738789805778 1101 78-79 0.6763253763876662 1101 80-81 0.8349166622793831 1101 82-83 0.7560881668939281 1101 84-85 0.7372907133343709 1101 86-87 0.6526805838108096 1101 88-89 0.665703233249701 1101 90-91 0.707183019740242 1101 92-93 0.6519755754104217 1101 94-95 0.7154228773863203 1101 96-97 0.7536102420208017 1101 98-99 0.7208256219162372 1101 100-101 0.6263345558795201 2304 1 0.0401702071024701 2304 2 -0.01447252954726963 2304 3 0.05732115423752049 2304 4 0.030478272874688628 2304 5 0.05718092677120268 2304 6 0.14646653056567516 2304 7 0.3091661332267108 2304 8 0.2141571278074963 2304 9 0.2085274535194941 2304 10-11 0.222419344176366 2304 12-13 0.23401990983789034 2304 14-15 0.3630911843480291 2304 16-17 0.35703648431780266 2304 18-19 0.3836457953199215 2304 20-21 0.3810304398206412 2304 22-23 0.2756880830120423 2304 24-25 0.33885723990167804 2304 26-27 0.3914567103594564 2304 28-29 0.39353603816492466 2304 30-31 0.25635966026791124 2304 32-33 0.3308163749786175 2304 34-35 0.2714070702022511 2304 36-37 0.2779581790382437 2304 38-39 0.30162761687256534 2304 40-41 0.2960027106616039 2304 42-43 0.42797141589387167 2304 44-45 0.33007137522705676 2304 46-47 0.3358329160625715 2304 48-49 0.3145317410622539 2304 50-51 0.3031984505887806 2304 52-53 0.3029320629108483 2304 54-55 0.4818265187407391 2304 56-57 0.4326032419368815 2304 58-59 0.4189469582992871 2304 60-61 0.40750614680428754 2304 62-63 0.5074354754124499 2304 64-65 0.525108091896783 2304 66-67 0.4029022711986272 2304 68-69 0.3323836153811399 2304 70-71 0.3658430008868976 2304 72-73 0.2967399129979498 2304 74-75 0.41855989281642536 2304 76-77 0.5292562210933127 2304 78-79 0.5417735710209115 2304 80-81 0.5045659001290019 2304 82-83 0.510597248535813 2304 84-85 0.46877119040764015 2304 86-87 0.4656465599984152 2304 88-89 0.48588290179064586 2304 90-91 0.5649105184716987 2304 92-93 0.5697456957971632 2304 94-95 0.4547644612001669 2304 96-97 0.564088022176584 2304 98-99 0.45735830487082296 2304 100-101 0.41487058801971344 2202 1 0.04568777738579044 2202 2 -0.03872924010532586 2202 3 -0.017549216270460022 2202 4 -0.041749422746335085 2202 5 -0.00756651960216459 2202 6 0.13529426147464818 2202 7 0.17679087484364686 2202 8 0.12388467907496903 2202 9 0.0922587383139728 2202 10-11 0.1657767739494176 2202 12-13 0.15283226505911784 2202 14-15 0.1886762397465631 2202 16-17 0.2773319313900231 2202 18-19 0.28772981373546 2202 20-21 0.23337941870035905 2202 22-23 0.30838392294553074 2202 24-25 0.3629963403005192 2202 26-27 0.3323734915988936 2202 28-29 0.22857970705133823 2202 30-31 0.26705673412283915 2202 32-33 0.1817074956949014 2202 34-35 0.26371351100591056 2202 36-37 0.23705917509835928 2202 38-39 0.23788592019195676 2202 40-41 0.4133516414071252 2202 42-43 0.35321823400331454 2202 44-45 0.3675906923795509 2202 46-47 0.25405315211930457 2202 48-49 0.34074346440606007 2202 50-51 0.39612128940333946 2202 52-53 0.2854312359537019 2202 54-55 0.39846705145034633 2202 56-57 0.43632853417170736 2202 58-59 0.37180373426102165 2202 60-61 0.3713495566169982 2202 62-63 0.4366183076348733 2202 64-65 0.43364584636562853 2202 66-67 0.4106641005582432 2202 68-69 0.3808063461927773 2202 70-71 0.37588975340051434 2202 72-73 0.4425795200331635 2202 74-75 0.5138277784080998 2202 76-77 0.525667312332267 2202 78-79 0.6069821350952793 2202 80-81 0.5478289888496022 2202 82-83 0.5101816475447656 2202 84-85 0.520816033748698 2202 86-87 0.4114637155218652 2202 88-89 0.32875316061946336 2202 90-91 0.38949883340780644 2202 92-93 0.3002689015060085 2202 94-95 0.44216221452359505 2202 96-97 0.46678011940717923 2202 98-99 0.4051848618741438 2202 100-101 0.33574945873161255 1102 1 -0.032657210548777016 1102 2 -0.13792933036024024 1102 3 -0.04722065988320878 1102 4 -0.08787409879528241 1102 5 -0.13973049917467506 1102 6 0.013760455068435817 1102 7 -0.04534455632607148 1102 8 0.1469121441317327 1102 9 -0.0753981689506773 1102 10-11 0.020661420472990244 1102 12-13 0.05069215172311203 1102 14-15 0.06321155417016655 1102 16-17 0.08525633330681615 1102 18-19 0.15553863587344807 1102 20-21 0.08832025576990077 1102 22-23 0.14050410872140162 1102 24-25 -0.014683973244999038 1102 26-27 0.10920183885335177 1102 28-29 0.11862141564578366 1102 30-31 0.150650347852455 1102 32-33 0.10958973466230049 1102 34-35 0.15553254205861577 1102 36-37 0.07806865974048094 1102 38-39 0.08651110966550135 1102 40-41 0.19760322271462627 1102 42-43 0.21872385633080427 1102 44-45 0.16011210263150844 1102 46-47 0.106888764572453 1102 48-49 0.046247176552526525 1102 50-51 0.03424280576013672 1102 52-53 0.16362483407842632 1102 54-55 0.3163062782656354 1102 56-57 0.3346875987087685 1102 58-59 0.2860164524682318 1102 60-61 0.34571750529855194 1102 62-63 0.41905395331404804 1102 64-65 0.33072697634289483 1102 66-67 0.2835163501735636 1102 68-69 0.34514103140127617 1102 70-71 0.3321352227588079 1102 72-73 0.4721176237691225 1102 74-75 0.444224581499455 1102 76-77 0.4508706685521915 1102 78-79 0.4696228709290722 1102 80-81 0.459517394253794 1102 82-83 0.3586692791313659 1102 84-85 0.3602027746280676 1102 86-87 0.3493153877423616 1102 88-89 0.37835028289341466 1102 90-91 0.28237921032499 1102 92-93 0.37119241435027206 1102 94-95 0.41620010828665954 1102 96-97 0.5259918457694255 1102 98-99 0.4408506317206786 1102 100-101 0.4333888677299349 2305 1 0.007846933707110537 2305 2 -0.031246528439261567 2305 3 0.01900375815919375 2305 4 -0.06938309299744105 2305 5 -0.08473938213553822 2305 6 0.14460212133015915 2305 7 -0.09573653753903955 2305 8 0.19714331030444043 2305 9 2.857907078492872E-4 2305 10-11 0.06059944348959334 2305 12-13 0.06440860691509442 2305 14-15 0.002936148607922462 2305 16-17 0.009358825220843414 2305 18-19 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-0.2472088988902854 1213 54-55 -0.43711478813074933 1213 56-57 -1.1930217487719617 1213 58-59 -0.7612654582181904 1213 60-61 -0.5309524477346557 1213 62-63 -0.5138001477888707 1213 64-65 -0.36842469589420546 1213 66-67 -0.24005458489249776 1213 68-69 -0.21594895947428228 1213 70-71 -0.2668673643834154 1213 72-73 -0.07181667556273652 1213 74-75 -0.17133916693813944 1213 76-77 -0.17479937046226013 1213 78-79 -0.28007540996406277 1213 80-81 -0.43696558608968417 1213 82-83 -0.4210734082749994 1213 84-85 -0.3943250707952082 1213 86-87 -0.4088224055585137 1213 88-89 -0.3334912752642438 1213 90-91 -0.29983348261654186 1213 92-93 -0.31823794633590197 1213 94-95 -0.27461536967216205 1213 96-97 -0.279765912446706 1213 98-99 -0.3032218511502407 1213 100-101 -0.11812205365986017 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3088.0 3 655.0 4 924.0 5 1159.0 6 3186.0 7 7652.0 8 24542.0 9 64602.0 10 98580.0 11 87613.0 12 59082.0 13 42803.0 14 33456.0 15 28587.0 16 26408.0 17 26532.0 18 27683.0 19 29777.0 20 32624.0 21 35714.0 22 39991.0 23 44021.0 24 49865.0 25 57569.0 26 69252.0 27 83438.0 28 102173.0 29 123641.0 30 147781.0 31 189920.0 32 257337.0 33 365814.0 34 550140.0 35 898977.0 36 1621116.0 37 2473763.0 38 684082.0 39 1153.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.611760560681226 27.837640380147395 17.286457228284366 17.264141830887016 2 31.937955749912277 27.428561945574042 25.322123861897474 15.311358442616207 3 34.24526393127277 22.408950678994184 24.722848737655127 18.622936652077925 4 30.554858656547935 20.950658038433655 26.27077577948784 22.22370752553057 5 33.78096142722813 19.771616094654554 25.778537007139818 20.6688854709775 6 32.13109525699695 19.85260651432977 27.624894086799205 20.391404141874073 7 33.32537990157062 21.156319275791745 23.16606920534962 22.35223161728802 8 28.968732855964284 26.72321518105592 22.480299971514295 21.827751991465497 9 28.995415660675427 24.484032310948166 25.276020149631883 21.244531878744525 10-11 24.673360259892053 29.712014166393736 28.15922411457804 17.455401459136173 12-13 25.11669160845277 26.918617160843134 28.650060629973417 19.314630600730684 14-15 23.743554762969275 26.936954592227096 29.7239429626445 19.595547682159122 16-17 25.226776717433356 26.831700291379935 29.76063588632123 18.18088710486548 18-19 24.956015653026718 26.633870802560384 30.1785734085249 18.231540135887997 20-21 24.326231944115275 28.453263208793768 29.00193773959469 18.218567107496263 22-23 26.09626196095004 27.18554873305098 28.144351600344258 18.573837705654725 24-25 23.68241182344995 29.01542733757447 28.44798579379303 18.854175045182554 26-27 23.076344181532544 29.786017181412877 28.649011917529503 18.488626719525072 28-29 23.55278992534517 29.371173038908445 28.54377134111849 18.5322656946279 30-31 24.587380224412005 27.992176003942472 29.09554658153965 18.324897190105872 32-33 22.234206429129525 29.516091472552404 29.877889000781334 18.37181309753674 34-35 24.923807011027883 28.268284659002795 28.703154959558656 18.104753370410663 36-37 22.682678304626613 28.831275897741044 30.28321387996698 18.202831917665364 38-39 22.84074361394772 29.6320811200436 28.93172811937052 18.59544714663816 40-41 25.38122093626883 27.39350299358785 28.13791466109401 19.087361409049315 42-43 25.040790651115163 27.31160374755065 28.59917263670099 19.048432964633196 44-45 23.134025671293877 28.357736503145404 29.41943427521181 19.08880355034891 46-47 23.594007381099182 28.81582626467729 28.537667477721502 19.052498876502032 48-49 21.57557907724238 28.870574465426923 29.66918667660563 19.884659780725062 50-51 21.277093449699795 28.102904355157783 30.006748030409657 20.613254164732766 52-53 24.398722715602418 27.781509503686696 27.90712423969281 19.912643541018078 54-55 24.888928441030874 27.716636979649685 28.096010819171013 19.298423760148424 56-57 25.033742624096377 27.370284482851886 28.22123039704613 19.374742496005606 58-59 24.208685461820707 27.651604234464905 28.0811719527751 20.058538350939283 60-61 21.91187866730794 27.824923953748964 27.87525994830522 22.387937430637876 62-63 23.407014554896108 27.851003471346363 28.173492994633996 20.56848897912353 64-65 21.781167336562575 28.561063588580854 29.529370118609666 20.128398956246908 66-67 21.318422106338787 28.19721238794486 28.75565141888759 21.728714086828763 68-69 20.982694928533494 27.70237131798472 31.49124218017657 19.82369157330521 70-71 20.686509560495036 30.34496046456747 29.54166380394488 19.426866170992614 72-73 20.72932389128381 31.976645061592322 27.705595586414145 19.58843546070972 74-75 20.899048181196857 31.870605933155222 27.56383494797616 19.66651093767176 76-77 21.08213259875927 31.547351553264964 27.623423175191924 19.747092672783843 78-79 20.945647460510298 31.298806869827757 27.950709221256453 19.804836448405496 80-81 20.58014756139613 31.57875301842758 27.74986348249508 20.091235937681216 82-83 20.524939145810205 31.296921169775104 27.68636575078922 20.491773933625467 84-85 20.809990439042124 30.86480862935162 27.900481418342014 20.42471951326424 86-87 20.869166289540566 30.500536582905223 28.475733997362152 20.154563130192056 88-89 21.154070397260252 30.761448109028045 28.14014903049492 19.94433246321678 90-91 21.424773087424256 30.309381993121022 27.740033627035576 20.525811292419146 92-93 21.22075374688633 30.43060889464389 27.564062965063712 20.784574393406068 94-95 20.866649902711107 30.43928075580945 27.565558805787425 21.128510535692023 96-97 20.887080512101235 30.760244190614465 27.62263677467635 20.730038522607945 98-99 21.27636509300859 30.432704228269454 27.486264403648597 20.804666275073362 100-101 21.5461786988288 30.3444311161375 27.042821358007917 21.066568827025783 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1765.0 1 1292.0 2 665.0 3 458.5 4 460.0 5 649.5 6 957.5 7 1336.0 8 1805.0 9 2338.5 10 2755.0 11 3004.0 12 3035.0 13 2872.0 14 2608.0 15 2267.5 16 1935.0 17 1652.5 18 1572.0 19 1678.0 20 1994.0 21 2542.5 22 3429.5 23 4850.5 24 6797.0 25 9169.0 26 12619.0 27 17511.0 28 23295.0 29 29784.5 30 37413.0 31 46452.5 32 62855.0 33 91488.5 34 133237.5 35 193098.0 36 264726.5 37 345858.5 38 430204.0 39 480187.5 40 516624.0 41 525051.5 42 496042.5 43 490977.5 44 486497.0 45 482426.0 46 502360.5 47 481439.0 48 404416.5 49 331330.5 50 275298.0 51 230486.0 52 191959.5 53 157562.5 54 131451.5 55 108458.0 56 84423.5 57 62688.5 58 47118.0 59 36459.0 60 28328.0 61 21613.0 62 16128.0 63 12054.5 64 9100.0 65 7065.5 66 5568.5 67 4319.5 68 3335.5 69 2527.0 70 1893.5 71 1522.5 72 1242.0 73 951.0 74 722.5 75 603.5 76 471.5 77 376.5 78 317.0 79 251.0 80 213.0 81 185.0 82 161.5 83 142.5 84 128.0 85 111.5 86 89.5 87 76.5 88 67.0 89 58.0 90 47.0 91 46.5 92 44.5 93 31.5 94 24.0 95 24.5 96 20.0 97 14.5 98 10.0 99 5.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2301094738346814 2 0.2240342120623727 3 0.20530811107007993 4 0.19433690304596948 5 0.1979105864414452 6 0.12479302417001203 7 0.09986062634757643 8 0.09573897816479444 9 0.08874647098764697 10-11 0.0694783613470404 12-13 0.09734117955376607 14-15 0.09972363515074988 16-17 0.08982453214528213 18-19 0.08406494573957378 20-21 0.0783351400288277 22-23 0.1573194992078335 24-25 0.624572647027291 26-27 0.35692758526212964 28-29 0.18268669517671865 30-31 0.31379322667873777 32-33 0.3516980952267502 34-35 0.3329779503734499 36-37 0.34240056225952087 38-39 0.3504413498993413 40-41 0.35225201615304896 42-43 0.3724254589205094 44-45 0.34932755190775133 46-47 0.372991292124793 48-49 0.3611207071128212 50-51 0.3666360918198387 52-53 0.3601736810130201 54-55 0.3431867726065256 56-57 0.35187082325753155 58-59 0.3422576149237019 60-61 0.32080360227286264 62-63 0.30853991208738846 64-65 0.3080812893849691 66-67 0.32163746173180696 68-69 0.32001143578686553 70-71 0.3052521233635508 72-73 0.2966991077703789 74-75 0.30328659749603915 76-77 0.3047994568001239 78-79 0.3082063683038107 80-81 0.31836158528595426 82-83 0.3455334913695546 84-85 0.3318820207988374 86-87 0.343049781409699 88-89 0.3450093511382182 90-91 0.32550895207690567 92-93 0.3043706147926668 94-95 0.3044003954876291 96-97 0.31254839362931375 98-99 0.3338594589443339 100-101 0.3410425625692401 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8394700.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.482915020223274 #Duplication Level Percentage of deduplicated Percentage of total 1 89.89094790791985 45.37957084322835 2 4.792217534081663 4.83850221062937 3 1.5330754149024706 2.3218234767034494 4 0.8570483641875858 1.7306519895001304 5 0.46039784836263636 1.1621112727192309 6 0.3186062782049481 0.9650504200518008 7 0.312735520947915 1.1051460509473194 8 0.1988542470012916 0.8030993642221355 9 0.13840478317691784 0.6288369216761496 >10 1.1876854851864458 12.404417035826173 >50 0.18054711384215028 6.376844476708065 >100 0.10941548158405938 10.318643019875164 >500 0.011028987204153516 3.9931494223749384 >1k 0.008963677217884295 7.516325672185792 >5k 4.76249526375906E-5 0.13294903907747666 >10k+ 2.373122740471388E-5 0.3228787842745615 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT 26951 0.32104780397155347 Illumina Single End PCR Primer 1 (97% over 34bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0028589467163805736 0.0 0.0 2 0.003049543164139278 0.0 0.0 3 0.0032758764458527406 0.0 0.0 4 0.003454560615626526 0.0 0.0 5 0.0040144376809177216 0.0 1.1912277984919055E-5 6 0.004359893742480374 0.0 1.1912277984919055E-5 7 0.0050627181435905985 0.0 1.1912277984919055E-5 8 0.005837016212610338 0.0 1.1912277984919055E-5 9 0.006432630111856291 0.0 1.1912277984919055E-5 10-11 0.010208822233075631 0.0 1.7868416977378582E-5 12-13 0.013562128485830344 0.0 2.382455596983811E-5 14-15 0.017808855587453987 0.0 2.382455596983811E-5 16-17 0.02212110021799469 0.0 2.382455596983811E-5 18-19 0.027094476276698393 0.0 2.382455596983811E-5 20-21 0.033360334496765814 0.0 2.382455596983811E-5 22-23 0.04037071009089068 0.0 2.382455596983811E-5 24-25 0.048899901128092726 0.0 2.382455596983811E-5 26-27 0.058590539268824376 0.0 2.382455596983811E-5 28-29 0.06923415964834956 0.0 2.382455596983811E-5 30-31 0.10276126603690425 0.0 2.382455596983811E-5 32-33 0.11705599961880711 0.0 2.382455596983811E-5 34-35 0.13052878601975054 0.0 2.382455596983811E-5 36-37 0.14451380037404554 5.956138992459528E-6 2.382455596983811E-5 38-39 0.1600712354223498 1.1912277984919055E-5 2.382455596983811E-5 40-41 0.17617067911896792 1.1912277984919055E-5 2.382455596983811E-5 42-43 0.1936579032008291 1.1912277984919055E-5 3.573683395475717E-5 44-45 0.21318212681811144 1.1912277984919055E-5 3.573683395475717E-5 46-47 0.23298628896803936 1.1912277984919055E-5 4.1692972947216696E-5 48-49 0.25284405636889945 1.1912277984919055E-5 4.764911193967622E-5 50-51 0.2737620165104173 1.1912277984919055E-5 4.764911193967622E-5 52-53 0.29597245881329887 1.1912277984919055E-5 4.764911193967622E-5 54-55 0.3187368220424792 1.1912277984919055E-5 4.764911193967622E-5 56-57 0.3422159219507547 1.1912277984919055E-5 4.764911193967622E-5 58-59 0.36803578448306673 1.1912277984919055E-5 4.764911193967622E-5 60-61 0.39445721705361714 1.1912277984919055E-5 4.764911193967622E-5 62-63 0.42236172823329005 1.1912277984919055E-5 4.764911193967622E-5 64-65 0.45191013377488176 1.1912277984919055E-5 4.764911193967622E-5 66-67 0.4830786091224225 1.1912277984919055E-5 4.764911193967622E-5 68-69 0.516266215588407 1.1912277984919055E-5 7.147366790951434E-5 70-71 0.5517588478444733 1.1912277984919055E-5 7.147366790951434E-5 72-73 0.5894433392497647 1.1912277984919055E-5 7.147366790951434E-5 74-75 0.62987361073058 1.1912277984919055E-5 7.147366790951434E-5 76-77 0.674395749699215 1.1912277984919055E-5 7.147366790951434E-5 78-79 0.722432010673401 1.1912277984919055E-5 7.147366790951434E-5 80-81 0.7737620165104173 1.1912277984919055E-5 7.147366790951434E-5 82-83 0.830541889525534 1.1912277984919055E-5 9.529822387935244E-5 84-85 0.8913659809165306 1.1912277984919055E-5 9.529822387935244E-5 86-87 0.9554897733093499 1.1912277984919055E-5 1.072105018642715E-4 88-89 1.0293697213718178 1.1912277984919055E-5 1.3699119682656914E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 4720 0.0 84.51239 9 CGGGAGA 6575 0.0 82.38077 4 AGAGCGG 5415 0.0 76.11787 8 ATTCGGA 1005 0.0 73.6354 2 GATCGGG 9240 0.0 73.477684 1 ATCGGGA 9170 0.0 72.01086 2 CGGAAAG 2345 0.0 68.96944 5 AAGCGGC 1595 0.0 68.61224 9 GAGAGCG 7175 0.0 68.40749 7 TCGGGAA 2090 0.0 66.72446 3 TTCGGAG 970 0.0 64.54851 3 GATCCGG 1200 0.0 63.65498 1 GTTCGGA 1045 0.0 61.73774 2 TCGCGCG 40 7.6081033E-6 59.297733 2 AAAGCGG 2135 0.0 57.251606 8 TTCGGAA 1410 0.0 55.843548 3 GAACCGG 535 0.0 54.98265 1 AAGGGGC 4440 0.0 53.56376 9 AGAGCGT 20090 0.0 52.91865 8 GAGCGTC 20005 0.0 52.739006 9 >>END_MODULE