FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n02_30484-1.34200000035803.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n02_30484-1.34200000035803.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8394700
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT269510.32104780397155347Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC47200.084.512399
CGGGAGA65750.082.380774
AGAGCGG54150.076.117878
ATTCGGA10050.073.63542
GATCGGG92400.073.4776841
ATCGGGA91700.072.010862
CGGAAAG23450.068.969445
AAGCGGC15950.068.612249
GAGAGCG71750.068.407497
TCGGGAA20900.066.724463
TTCGGAG9700.064.548513
GATCCGG12000.063.654981
GTTCGGA10450.061.737742
TCGCGCG407.6081033E-659.2977332
AAAGCGG21350.057.2516068
TTCGGAA14100.055.8435483
GAACCGG5350.054.982651
AAGGGGC44400.053.563769
AGAGCGT200900.052.918658
GAGCGTC200050.052.7390069
Produced by FastQC (version 0.11.2)