##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n02_30423-1.34200000035748.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2610328 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.981005835282 33.0 33.0 33.0 27.0 33.0 2 30.43833533563598 33.0 33.0 33.0 22.0 33.0 3 30.28386241116059 33.0 33.0 33.0 22.0 33.0 4 33.54842226723998 37.0 37.0 37.0 15.0 37.0 5 33.662857311418335 37.0 37.0 37.0 22.0 37.0 6 34.369087333086114 37.0 37.0 37.0 27.0 37.0 7 33.57054094351361 37.0 37.0 37.0 22.0 37.0 8 34.01885778338967 37.0 37.0 37.0 27.0 37.0 9 33.3403219825248 37.0 37.0 37.0 15.0 37.0 10-11 33.34629843452623 37.0 37.0 37.0 15.0 37.0 12-13 33.33241761188633 37.0 37.0 37.0 15.0 37.0 14-15 34.73300290231725 40.0 37.0 40.0 15.0 40.0 16-17 34.583468820776545 38.5 37.0 40.0 15.0 40.0 18-19 34.70515372014552 38.5 37.0 40.0 15.0 40.0 20-21 34.697937385646554 40.0 37.0 40.0 15.0 40.0 22-23 35.61087476363124 37.0 37.0 40.0 24.5 40.0 24-25 34.680288454171276 37.0 37.0 40.0 15.0 40.0 26-27 34.605396524881165 37.0 37.0 40.0 18.5 40.0 28-29 34.304261571725846 37.0 37.0 40.0 15.0 40.0 30-31 34.272829314936665 37.0 37.0 40.0 15.0 40.0 32-33 34.075271958160045 37.0 37.0 40.0 15.0 40.0 34-35 34.43171126387182 37.0 37.0 40.0 18.5 40.0 36-37 33.88274825998879 37.0 33.0 40.0 15.0 40.0 38-39 33.8181105975954 37.0 33.0 40.0 15.0 40.0 40-41 34.47102777888449 37.0 33.0 40.0 18.5 40.0 42-43 33.96899374331502 37.0 33.0 40.0 15.0 40.0 44-45 33.40973528997122 37.0 33.0 40.0 15.0 40.0 46-47 33.25111441933734 37.0 33.0 40.0 10.5 40.0 48-49 33.13094216512255 37.0 33.0 40.0 10.5 40.0 50-51 32.21687944963239 37.0 33.0 38.5 15.0 38.5 52-53 32.74348491837041 37.0 33.0 38.5 15.0 40.0 54-55 33.34262322589345 37.0 33.0 40.0 15.0 40.0 56-57 33.68501161539852 37.0 33.0 40.0 18.5 40.0 58-59 33.07209113184244 37.0 33.0 40.0 15.0 40.0 60-61 32.88079352479841 37.0 33.0 40.0 10.5 40.0 62-63 32.4370090655274 37.0 33.0 38.5 6.0 40.0 64-65 32.20590956385558 37.0 33.0 37.0 6.0 40.0 66-67 32.08383812302515 37.0 33.0 37.0 10.5 40.0 68-69 32.287517890472 37.0 33.0 37.0 15.0 38.5 70-71 31.81045638709005 37.0 33.0 37.0 15.0 37.0 72-73 32.15664506529448 37.0 33.0 37.0 18.5 37.0 74-75 32.2989723896767 37.0 33.0 37.0 22.0 37.0 76-77 31.928301922210544 35.0 33.0 37.0 22.0 37.0 78-79 31.43985564266253 33.0 33.0 37.0 18.5 37.0 80-81 30.982312567616024 33.0 33.0 37.0 15.0 37.0 82-83 30.449391417477038 33.0 27.0 37.0 15.0 37.0 84-85 30.225932909580713 33.0 27.0 37.0 15.0 37.0 86-87 30.072268695734792 33.0 27.0 37.0 6.0 37.0 88-89 29.992479680714453 33.0 27.0 37.0 6.0 37.0 90-91 29.877878948545927 33.0 27.0 37.0 6.0 37.0 92-93 29.681224926522646 33.0 27.0 37.0 6.0 37.0 94-95 29.49500120291396 33.0 27.0 37.0 6.0 37.0 96-97 29.296845453904645 33.0 27.0 37.0 6.0 37.0 98-99 29.198054612293934 33.0 27.0 37.0 6.0 37.0 100-101 27.95643382747302 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.3321190998087289 2306 2 -0.472194852275976 2306 3 -0.5944218262126704 2306 4 -0.2976152345595793 2306 5 -0.39351756418244577 2306 6 -0.6034539093899696 2306 7 0.19013424232309717 2306 8 -0.6306619244545857 2306 9 -0.24949011119716147 2306 10-11 -0.3092561132710685 2306 12-13 -0.2699458858186361 2306 14-15 0.12868194377502817 2306 16-17 0.06358635654751765 2306 18-19 0.06823016981527275 2306 20-21 0.1180199925159684 2306 22-23 0.023710985477130464 2306 24-25 0.30510544610403656 2306 26-27 -0.15042077568589463 2306 28-29 0.04438980718193619 2306 30-31 0.2669648078207345 2306 32-33 0.09776310038539293 2306 34-35 -0.13122586275223114 2306 36-37 0.19392908129103148 2306 38-39 -0.0029667500249033196 2306 40-41 -0.11651005836797168 2306 42-43 0.49520759523223745 2306 44-45 0.5566372119402558 2306 46-47 0.2698084729974042 2306 48-49 0.1983009048110631 2306 50-51 0.08936314166571435 2306 52-53 0.04954760638263167 2306 54-55 0.36477625196337016 2306 56-57 0.2821654762874246 2306 58-59 0.5309741036488305 2306 60-61 0.32713169712828005 2306 62-63 0.37032713571368703 2306 64-65 0.28517766762276153 2306 66-67 0.18982155230584397 2306 68-69 0.007605552780134417 2306 70-71 0.26778227369822005 2306 72-73 -0.021242495789003613 2306 74-75 0.1737426678166898 2306 76-77 0.35867457288281557 2306 78-79 0.40926408153237404 2306 80-81 0.4976027224473434 2306 82-83 0.6224912210080866 2306 84-85 0.6470237385433748 2306 86-87 0.4875883119255384 2306 88-89 0.607194350025086 2306 90-91 0.6466549135007504 2306 92-93 0.5478339058684334 2306 94-95 0.5195033356131873 2306 96-97 0.570497011601443 2306 98-99 0.6628038338420517 2306 100-101 0.5774915505055311 2307 1 -0.13897846946680303 2307 2 -0.19475872131624783 2307 3 -0.23540220835586467 2307 4 -3.1937503529633204E-4 2307 5 0.006643305364271157 2307 6 -0.20692253486324574 2307 7 0.31063957697856637 2307 8 -0.2652983153023314 2307 9 0.1420878573353903 2307 10-11 0.11148123385676456 2307 12-13 0.09078864538282261 2307 14-15 0.535300824809589 2307 16-17 0.480194683177956 2307 18-19 0.515379062099349 2307 20-21 0.39418558742828935 2307 22-23 0.19226882687450342 2307 24-25 0.6379506027816078 2307 26-27 0.274965864481274 2307 28-29 0.47816340386467715 2307 30-31 0.4673562712370938 2307 32-33 0.4510325895039955 2307 34-35 0.03837234640371179 2307 36-37 0.47537755267568826 2307 38-39 0.2595476310600091 2307 40-41 0.08421725923873424 2307 42-43 0.6046916281494958 2307 44-45 0.593455626538244 2307 46-47 0.3311862695953067 2307 48-49 0.3286431852716518 2307 50-51 0.3366310901817826 2307 52-53 0.2613855976979451 2307 54-55 0.38471063449525644 2307 56-57 0.2964293491579184 2307 58-59 0.5613199660812498 2307 60-61 0.41617313309468784 2307 62-63 0.5373871099802301 2307 64-65 0.4726502708580256 2307 66-67 0.32977770329623723 2307 68-69 0.08346657369175858 2307 70-71 0.24690806687445743 2307 72-73 -0.12519397815790256 2307 74-75 -0.06926823130789472 2307 76-77 0.030515947565579182 2307 78-79 0.24256957478762686 2307 80-81 0.4837764885112428 2307 82-83 0.4468628864051034 2307 84-85 0.370806187306993 2307 86-87 0.26763272128320637 2307 88-89 0.38107298005208534 2307 90-91 0.4177447479356253 2307 92-93 0.5253357535984549 2307 94-95 0.44152097168201365 2307 96-97 0.27808965699757593 2307 98-99 0.2538724530734804 2307 100-101 0.24528707027446828 2201 1 -0.13605493885870956 2201 2 -0.33670017674916153 2201 3 -0.23156926328588767 2201 4 -0.17747492997455794 2201 5 -0.18878836028552115 2201 6 -0.2589405798648201 2201 7 0.3098353171749224 2201 8 -0.3139514112403532 2201 9 -0.050979561822629194 2201 10-11 -0.09571562028563818 2201 12-13 -0.06337443909219331 2201 14-15 0.23365948770454992 2201 16-17 0.21757207977972826 2201 18-19 0.2628888611268465 2201 20-21 0.1756031415540562 2201 22-23 0.22099915948678017 2201 24-25 0.29588538840476986 2201 26-27 -0.008083320165681585 2201 28-29 0.22062115711407415 2201 30-31 0.42674404001702726 2201 32-33 0.2183906191599192 2201 34-35 0.08278170077722535 2201 36-37 0.42783840278754326 2201 38-39 0.27409075140316475 2201 40-41 0.1258307708034252 2201 42-43 0.7106881014240329 2201 44-45 0.6153659284592194 2201 46-47 0.4908006408860004 2201 48-49 0.3973057104625539 2201 50-51 0.29046992853480447 2201 52-53 0.27610089434963214 2201 54-55 0.4888281904698957 2201 56-57 0.6582214618652245 2201 58-59 0.7676380277226826 2201 60-61 0.37959606744119867 2201 62-63 0.5433838143639065 2201 64-65 0.47302813430025736 2201 66-67 0.3978890331906726 2201 68-69 0.34582571267154805 2201 70-71 0.47752609893391806 2201 72-73 0.21829197965081448 2201 74-75 0.28210663695990235 2201 76-77 0.5829915886623276 2201 78-79 0.6972733390932824 2201 80-81 0.8030011866827103 2201 82-83 0.753978621239721 2201 84-85 0.8765012942643047 2201 86-87 0.6478292102731658 2201 88-89 0.7028941893999878 2201 90-91 0.7475161116644351 2201 92-93 0.6364299167060423 2201 94-95 0.606687162263853 2201 96-97 0.7570754807629925 2201 98-99 0.786783550009897 2201 100-101 0.7347993375475674 1101 1 -0.18664286444663247 1101 2 -0.42988478697922616 1101 3 -0.31364152149450675 1101 4 -0.35631559176976424 1101 5 -0.3335979425950981 1101 6 -0.2503091621424929 1101 7 0.1765668657246522 1101 8 -0.35636661274646286 1101 9 -0.1754311237741959 1101 10-11 -0.21301854156583033 1101 12-13 -0.1570087685219761 1101 14-15 0.008183642910530864 1101 16-17 0.08751946961348267 1101 18-19 0.0689531899184459 1101 20-21 -0.14030216014670316 1101 22-23 0.10115248282192368 1101 24-25 0.18291413054714667 1101 26-27 0.0023958351998345506 1101 28-29 0.05445440003822455 1101 30-31 0.10285148601083449 1101 32-33 0.08808473033131037 1101 34-35 -0.2105798056524648 1101 36-37 -0.007731095281954481 1101 38-39 -0.09871649356316681 1101 40-41 -0.0376975006793856 1101 42-43 0.1372342978565939 1101 44-45 0.2391394215508953 1101 46-47 0.01588121142112442 1101 48-49 0.04318424838654522 1101 50-51 0.1722236938112971 1101 52-53 0.008172953126241111 1101 54-55 0.23630437828699513 1101 56-57 0.38982385824034793 1101 58-59 0.2601832589042772 1101 60-61 0.04991599628385757 1101 62-63 0.06370693411884787 1101 64-65 0.10814332623363043 1101 66-67 0.01032282176082333 1101 68-69 0.033264885906170605 1101 70-71 0.14153699589936153 1101 72-73 0.09360109927811067 1101 74-75 0.07994240070475911 1101 76-77 0.39287105195088756 1101 78-79 0.51201495497126 1101 80-81 0.6726115364066949 1101 82-83 0.4881586764425023 1101 84-85 0.41441631229295695 1101 86-87 0.24390957339898378 1101 88-89 0.3092303440315938 1101 90-91 0.2853234364035764 1101 92-93 0.38590341617953783 1101 94-95 0.3706543836174383 1101 96-97 0.5092343323536603 1101 98-99 0.5217403929212878 1101 100-101 0.44714058966154724 2304 1 0.007855205051434666 2304 2 -0.2532438603383014 2304 3 -0.19105464890763457 2304 4 -0.17870941416358477 2304 5 -0.12027747927463395 2304 6 0.12565608882275825 2304 7 0.22820779236557343 2304 8 0.03407740269754811 2304 9 -0.06635776470083243 2304 10-11 7.845472372594031E-4 2304 12-13 0.020838721825519713 2304 14-15 0.19206395179083557 2304 16-17 0.2902221723161773 2304 18-19 0.23956129802655113 2304 20-21 0.12441791707337302 2304 22-23 0.12552506969451116 2304 24-25 0.31008594521895816 2304 26-27 0.28738618269018446 2304 28-29 0.23374976683358994 2304 30-31 0.2570503277096776 2304 32-33 0.19981252955911089 2304 34-35 0.0908198082471543 2304 36-37 0.32890017884931666 2304 38-39 0.36460396975729026 2304 40-41 0.3877368622549753 2304 42-43 0.5995895102117998 2304 44-45 0.4138498462613214 2304 46-47 0.2830144160543284 2304 48-49 0.2743618545641553 2304 50-51 0.2914321880197903 2304 52-53 0.2960590535123444 2304 54-55 0.4539684709510823 2304 56-57 0.5321983756148612 2304 58-59 0.5883685870896045 2304 60-61 0.3655530619209202 2304 62-63 0.48190191481382527 2304 64-65 0.4032688088591101 2304 66-67 0.2608054472434489 2304 68-69 0.253583418724709 2304 70-71 0.4470389264012944 2304 72-73 0.31078256645957936 2304 74-75 0.6228960686584273 2304 76-77 0.6498691214489547 2304 78-79 0.6389454569017623 2304 80-81 0.7758933896885445 2304 82-83 0.6370486378324642 2304 84-85 0.5300051153817584 2304 86-87 0.589952044441457 2304 88-89 0.46367049847174613 2304 90-91 0.6670801931603343 2304 92-93 0.5857103659864897 2304 94-95 0.32432233728539117 2304 96-97 0.3646880003073285 2304 98-99 0.5272905859714818 2304 100-101 0.20920622672718636 2202 1 -0.0550058471368402 2202 2 -0.2204192866107313 2202 3 -0.17360418867666638 2202 4 -0.34164869612387605 2202 5 -0.23085718216547235 2202 6 0.013125388060046816 2202 7 -0.053860157505233985 2202 8 -0.027121536518819767 2202 9 -0.14878963619171515 2202 10-11 -0.07991369123367065 2202 12-13 -0.10498942207929218 2202 14-15 -0.08785656279105325 2202 16-17 0.052687480238574835 2202 18-19 0.13483609897860305 2202 20-21 0.045232926315968314 2202 22-23 0.12151088564009882 2202 24-25 0.25008806816699547 2202 26-27 0.1853777746077938 2202 28-29 0.20865971413960693 2202 30-31 0.18111006021822362 2202 32-33 0.1524174989834748 2202 34-35 0.20558154616490043 2202 36-37 0.2546712145890666 2202 38-39 0.11086518643089249 2202 40-41 0.300297940160263 2202 42-43 0.49462903933157065 2202 44-45 0.27375512480764286 2202 46-47 0.11520421969026273 2202 48-49 0.11841611142015296 2202 50-51 0.2706696915364688 2202 52-53 0.2541500313284004 2202 54-55 0.3019279597520992 2202 56-57 0.40215842363146237 2202 58-59 0.3387800161443053 2202 60-61 0.1907788840454927 2202 62-63 0.29317496303927015 2202 64-65 0.25898773744348347 2202 66-67 0.29931559510763606 2202 68-69 0.3665929694018999 2202 70-71 0.3297967480840107 2202 72-73 0.3045396105910143 2202 74-75 0.4535196587534003 2202 76-77 0.5348659629165091 2202 78-79 0.5883877946990417 2202 80-81 0.6168804828548993 2202 82-83 0.5162703702616191 2202 84-85 0.44908746246477804 2202 86-87 0.49271282616960477 2202 88-89 0.31706982585967936 2202 90-91 0.39442495042797887 2202 92-93 0.38957434715266004 2202 94-95 0.43379925065702807 2202 96-97 0.36087920716013855 2202 98-99 0.40939393988417905 2202 100-101 0.2409025528189055 1102 1 0.10266605260328276 1102 2 0.08755642336641145 1102 3 0.11815073828699596 1102 4 0.08971541766629088 1102 5 -0.05759407360405788 1102 6 0.20825972650500546 1102 7 0.2104668630090103 1102 8 0.4030642526008279 1102 9 0.19646984954382418 1102 10-11 0.2386261590186649 1102 12-13 0.238408066541119 1102 14-15 0.21484988492534285 1102 16-17 0.34154810446027284 1102 18-19 0.40657088104772043 1102 20-21 0.38839478699252794 1102 22-23 0.3469101908520784 1102 24-25 0.2776847426688107 1102 26-27 0.3980818383020619 1102 28-29 0.35463936320932277 1102 30-31 0.4053858788172562 1102 32-33 0.4719559937278035 1102 34-35 0.44312003684030543 1102 36-37 0.36396790216694086 1102 38-39 0.3966642194685548 1102 40-41 0.42488584556655695 1102 42-43 0.45104619252802536 1102 44-45 0.4006662033714363 1102 46-47 0.3363298554372065 1102 48-49 0.4108547108284242 1102 50-51 0.3983546987745328 1102 52-53 0.5441027929193396 1102 54-55 0.7313921614126357 1102 56-57 0.6897684072281365 1102 58-59 0.5747498424509985 1102 60-61 0.5314685008245732 1102 62-63 0.6029501565954192 1102 64-65 0.6200888560473885 1102 66-67 0.6721111837974263 1102 68-69 0.6968058353904425 1102 70-71 0.5866474825903261 1102 72-73 0.7359261959629535 1102 74-75 0.7349777000563549 1102 76-77 0.5812067108854215 1102 78-79 0.6066273210461866 1102 80-81 0.7085720270113711 1102 82-83 0.5143321732148856 1102 84-85 0.6374525836846239 1102 86-87 0.618535373914316 1102 88-89 0.452471370831784 1102 90-91 0.4151004702197092 1102 92-93 0.6267870461301968 1102 94-95 0.6560896171546595 1102 96-97 0.7711473144000749 1102 98-99 0.5914986448785449 1102 100-101 0.6455588680084539 2305 1 0.0965015174238637 2305 2 0.043389442664427236 2305 3 0.11219554168332024 2305 4 -0.08011087249179383 2305 5 -0.05564562722240396 2305 6 0.32511874529178186 2305 7 -0.09608732850598045 2305 8 0.37587461392151056 2305 9 0.04839797147209879 2305 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scores pass #Quality Count 2 911.0 3 264.0 4 371.0 5 383.0 6 1005.0 7 3068.0 8 13183.0 9 41436.0 10 66886.0 11 56073.0 12 34469.0 13 22490.0 14 16510.0 15 13035.0 16 11425.0 17 10716.0 18 10304.0 19 10343.0 20 10747.0 21 11141.0 22 11890.0 23 12803.0 24 13973.0 25 16133.0 26 19095.0 27 23474.0 28 28974.0 29 34665.0 30 41638.0 31 54444.0 32 76084.0 33 109267.0 34 168332.0 35 288482.0 36 529877.0 37 712205.0 38 134178.0 39 54.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.436714771058725 20.338811889095723 24.46850176014155 11.755971579704001 2 33.7831393763942 24.671860182067398 31.126614014812652 10.418386426725743 3 39.36755907252848 16.211864946806507 30.57886740130011 13.841708579364903 4 31.50509944601028 13.876112499102785 34.860166822567834 19.758621232319097 5 36.44292576422566 12.577528053659123 34.87374797807143 16.10579820404378 6 31.926600155952805 13.070387032213498 40.58773419290203 14.415278618931657 7 39.04219294569134 16.256629495604237 28.66120137906546 16.03997617963896 8 30.58968742007584 32.46910110313078 21.527710941313476 15.413500535479905 9 36.80745240640399 27.65842421602654 19.375880901368188 16.158242476201284 10-11 31.52252416644177 28.394419024019236 24.601027533796216 15.482029275742782 12-13 33.334656236818894 21.584627478047473 24.597492235131714 20.483224050001915 14-15 28.607802490495942 22.461111405097103 26.40833350589033 22.522752598516625 16-17 30.07083339084664 24.842701101034777 27.645803905690443 17.440661602428133 18-19 29.56984908246548 25.009120230809412 27.592613715331858 17.828416971393242 20-21 27.525322957443095 28.5829821061516 26.39585608474615 17.49583885165916 22-23 32.15955627781825 24.022513774713765 25.612056560305152 18.205873387162836 24-25 27.9780042433548 28.178493025098778 25.890477197219635 17.953025534326787 26-27 24.12476784458668 29.610171127036445 27.280735915840854 18.984325112536027 28-29 25.408298520155313 28.98487766202806 25.28731904886535 20.319504768951276 30-31 26.987045018048374 26.31479147979349 26.842650368027687 19.85551313413045 32-33 23.145089902596393 27.209896492570675 29.379039985793625 20.26597361903931 34-35 29.633227873336555 24.190166183561885 26.057816930028427 20.118789013073133 36-37 23.506994815118155 28.400012068143504 29.279487634284518 18.81350548245382 38-39 23.138797345405543 30.78766857389731 24.721891025714942 21.35164305498221 40-41 30.022307336167348 24.922793016095223 23.70445153108046 21.35044811665697 42-43 30.820268348006614 25.438295336588368 24.75915958632886 18.982276729076162 44-45 24.549959622725197 29.388640782930924 25.71050358783124 20.350896006512638 46-47 25.683369984775556 29.648588278714993 25.51974564411706 19.148296092392393 48-49 22.073780442332072 27.289580125117535 29.15052468022914 21.486114752321257 50-51 19.674608219523545 26.13957954947143 29.323434439853248 24.862377791151776 52-53 27.004920018466887 26.749520356821467 25.065550914060935 21.18000871065071 54-55 28.169213293435934 26.415512403623513 25.998788165531284 19.41648613740927 56-57 29.32068021345894 25.770782832034367 24.158943767634415 20.74959318687228 58-59 26.259507511847158 24.626579140547832 24.8867938306385 24.227119516966514 60-61 21.0036054805764 24.666955115359624 26.576614829157553 27.752824574906427 62-63 25.345042613079695 24.67516699824261 27.064358006732952 22.915432381944747 64-65 22.75639332402133 28.367203800464473 28.32175160393809 20.554651271576105 66-67 20.67613852618563 28.024152992004915 26.57926180362399 24.720446678185475 68-69 19.7095324076191 23.835168969040435 34.879146991364706 21.57615163197576 70-71 19.3952775726579 31.487055932540702 29.49342519288739 19.624241301914005 72-73 21.27265270920111 35.112191477039715 25.061279606150915 18.553876207608255 74-75 22.376485788659704 33.99971992438343 24.958175950878807 18.66561833607806 76-77 21.455971597020238 32.81545382124157 24.98296562716171 20.74560895457648 78-79 21.725946170968637 33.86566966626139 24.905287330796135 19.50309683197384 80-81 20.97190327038532 34.40405088739003 24.656353344477782 19.96769249774687 82-83 20.64725502465243 33.22420258193262 25.571629643181648 20.556912750233302 84-85 21.251182196855137 31.880616275713862 25.855284117307747 21.012917410123254 86-87 19.585004653400905 32.15773866431348 26.98042420029821 21.27683248198741 88-89 19.475134909825986 32.25302340025608 26.992938240980724 21.2789034489372 90-91 21.025834542034858 30.882978799032628 25.922098842547804 22.169087816384707 92-93 21.205727832484165 31.289638277039895 24.900603553549026 22.604030336926918 94-95 21.545955729451315 32.351705533559254 24.742084260767054 21.36025447622238 96-97 20.898283659221633 33.37162793020906 23.727402665765016 22.002685744804293 98-99 21.285401626759874 30.991754752402382 25.272635039708234 22.45020858112951 100-101 22.69648925515143 31.594493711898007 24.83610041592714 20.872916617023417 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 629.0 1 442.5 2 211.5 3 211.5 4 232.0 5 238.5 6 332.5 7 466.5 8 607.5 9 733.5 10 838.5 11 953.0 12 1021.0 13 957.0 14 844.5 15 789.5 16 723.0 17 607.0 18 543.5 19 514.0 20 504.5 21 558.0 22 613.5 23 666.0 24 798.5 25 999.0 26 1194.5 27 1478.5 28 1901.5 29 2311.5 30 2917.0 31 4182.0 32 6541.5 33 10602.0 34 17121.0 35 28128.5 36 44147.5 37 64920.0 38 89392.0 39 107267.0 40 115921.5 41 119092.5 42 147831.5 43 184555.5 44 178346.5 45 159647.5 46 159980.0 47 154152.5 48 132790.0 49 116194.5 50 102941.5 51 96437.0 52 95697.5 53 97159.0 54 84079.5 55 56638.5 56 40671.0 57 33882.0 58 29207.0 59 23881.5 60 18613.5 61 14697.0 62 11450.5 63 8903.5 64 7003.5 65 5349.0 66 4091.0 67 3211.0 68 2546.5 69 1947.5 70 1450.0 71 1149.0 72 804.5 73 507.0 74 372.5 75 289.0 76 215.0 77 170.0 78 126.5 79 91.0 80 71.0 81 61.0 82 56.5 83 40.0 84 29.5 85 31.0 86 31.0 87 26.0 88 15.5 89 10.5 90 13.0 91 9.0 92 3.0 93 2.5 94 2.0 95 1.5 96 1.5 97 2.0 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22954969643661638 2 0.22211768023022394 3 0.20342271162857697 4 0.19327073072809242 5 0.19698673883128864 6 0.11986999334949477 7 0.0948156706743367 8 0.09144444682813807 9 0.08443383360252045 10-11 0.0671180020288638 12-13 0.09301513066557153 14-15 0.09554354855022051 16-17 0.08565973318295632 18-19 0.0810434550753775 20-21 0.07560352568719333 22-23 0.15371631457809135 24-25 0.6098275772240117 26-27 0.35095206426165604 28-29 0.1729284595652347 30-31 0.29645699697509276 32-33 0.3324869518313407 34-35 0.3154967498337374 36-37 0.32319693157335017 38-39 0.33137598033657073 40-41 0.3339618622640526 42-43 0.3535187915081936 44-45 0.33011177139424625 46-47 0.35428497874596604 48-49 0.34225583911293905 50-51 0.3484044916960627 52-53 0.34152796123705526 54-55 0.32515070902966986 56-57 0.3334255311976119 58-59 0.32325439561618313 60-61 0.3026822682819937 62-63 0.2913810065248505 64-65 0.2909021395012428 66-67 0.3033143727531559 68-69 0.30145636870155784 70-71 0.2878565452310974 72-73 0.2796774964678768 74-75 0.28576868500816754 76-77 0.28741598756937825 78-79 0.290672283329911 80-81 0.3002113144401776 82-83 0.3277749003190404 84-85 0.31340888961080754 86-87 0.3243845217918974 88-89 0.325533802648556 90-91 0.307145308942018 92-93 0.2870712033123807 94-95 0.28699458458860344 96-97 0.29490546781860366 98-99 0.3150178828101296 100-101 0.3229479207210741 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2610328.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.995672196307005 #Duplication Level Percentage of deduplicated Percentage of total 1 86.67450007605791 36.39953892972914 2 5.991378737178102 5.032239549009107 3 1.9267842800011985 2.427498030477832 4 1.1693857063527342 1.9643655518014538 5 0.7438651019525323 1.5619557489935516 6 0.5423871871651696 1.3666748709399286 7 0.32355540587031056 0.9511548733590841 8 0.27429216967535697 0.9215267234960087 9 0.23316985519411765 0.8812912324313282 >10 1.7140332240834295 14.335582896213783 >50 0.2105324728672911 6.298669854644292 >100 0.17372812125969436 14.483647796948858 >500 0.01502081998540476 4.26225905182896 >1k 0.00671805459653904 4.895598577580819 >5k 9.268396579453498E-5 0.2171139133422378 >10k+ 5.561037943740658E-4 4.000882399203671 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 30245 1.158666650321339 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 23075 0.8839885255799271 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT 15595 0.5974344986530428 Illumina Single End PCR Primer 1 (97% over 34bp) GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 11526 0.44155370512824443 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 11299 0.4328574799795275 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 11049 0.42328013950737226 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 5578 0.21368962061472735 No Hit TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 4967 0.19028260050077997 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 4881 0.18698799537835858 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGGGTAGATCT 4863 0.1862984268643634 Illumina Single End PCR Primer 1 (97% over 34bp) TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 3407 0.13051999595453137 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3297 0.12630596614678308 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004558814064745886 0.0 0.0 2 0.004865288959854853 0.0 0.0 3 0.005248382578741062 0.0 0.0 4 0.005478238750072788 0.0 0.0 5 0.006627519606731414 0.0 0.0 6 0.007432016206392453 0.0 0.0 7 0.008389750253607976 0.0 0.0 8 0.009347484300823498 0.0 0.0 9 0.010611693243147988 0.0 0.0 10-11 0.01620486007888664 0.0 0.0 12-13 0.020916911591187005 0.0 0.0 14-15 0.027123028217143595 0.0 0.0 16-17 0.03288858718138103 0.0 0.0 18-19 0.039209631893003485 0.0 0.0 20-21 0.0436343631911392 0.0 0.0 22-23 0.04826979597966233 0.0 0.0 24-25 0.05286691940629683 0.0 0.0 26-27 0.05836431283731393 0.0 0.0 28-29 0.06522168861537707 0.0 0.0 30-31 0.11801198929789666 0.0 0.0 32-33 0.12801073275082672 0.0 0.0 34-35 0.13594077066177124 0.0 0.0 36-37 0.1437558804870499 0.0 0.0 38-39 0.15323744755448357 0.0 0.0 40-41 0.16402153292613036 0.0 0.0 42-43 0.17532279468327353 0.0 0.0 44-45 0.18802234814935134 0.0 0.0 46-47 0.20160301693886745 0.0 0.0 48-49 0.21790365042247564 0.0 0.0 50-51 0.23498962582480057 0.0 0.0 52-53 0.251673352927295 0.0 0.0 54-55 0.2702725481242204 0.0 0.0 56-57 0.2915917080152379 0.0 0.0 58-59 0.31507534685296257 0.0 0.0 60-61 0.34242823124143784 0.0 0.0 62-63 0.37338219564744357 0.0 0.0 64-65 0.4062899375097689 0.0 0.0 66-67 0.441649478532966 0.0 0.0 68-69 0.4791543438219258 0.0 0.0 70-71 0.5197048033810311 0.0 0.0 72-73 0.5650439331762138 0.0 0.0 74-75 0.6153058159740845 0.0 0.0 76-77 0.67265493072135 0.0 0.0 78-79 0.7396580046645479 0.0 0.0 80-81 0.8142271775807485 0.0 0.0 82-83 0.8943703626517434 0.0 0.0 84-85 0.9860446656512132 0.0 0.0 86-87 1.0894799427504895 0.0 0.0 88-89 1.2045038018210739 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 3155 0.0 89.976974 9 CGGGAGA 4375 0.0 88.057236 4 ATCGGGA 5225 0.0 87.26682 2 AGAGCGG 3455 0.0 83.673065 8 TCGGGAG 4805 0.0 83.63289 3 AAGCGGC 870 0.0 81.1654 9 ATTCGGA 600 0.0 78.288155 2 ATCCGGA 750 0.0 75.28315 2 GTCCGGA 310 0.0 73.46688 2 GTTCGGA 440 0.0 73.32774 2 CGGAGAG 1460 0.0 72.787575 5 TTGGGAG 2375 0.0 70.5177 3 TCGGAGA 810 0.0 69.11687 4 TCGGGAA 1210 0.0 68.61827 3 GAGAGCG 5200 0.0 67.44228 7 AAGGGGC 2225 0.0 67.094124 9 AGAGCGT 10765 0.0 66.60837 8 CCGGAGA 850 0.0 66.422485 4 GGAGAGC 5385 0.0 66.36633 6 GAGCGTC 10750 0.0 66.17229 9 >>END_MODULE