FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n02_30423-1.34200000035748.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n02_30423-1.34200000035748.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2610328
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA302451.158666650321339No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC230750.8839885255799271No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT155950.5974344986530428Illumina Single End PCR Primer 1 (97% over 34bp)
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC115260.44155370512824443No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT112990.4328574799795275No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT110490.42328013950737226No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT55780.21368962061472735No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT49670.19028260050077997No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC48810.18698799537835858No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGGGTAGATCT48630.1862984268643634Illumina Single End PCR Primer 1 (97% over 34bp)
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG34070.13051999595453137No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC32970.12630596614678308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC31550.089.9769749
CGGGAGA43750.088.0572364
ATCGGGA52250.087.266822
AGAGCGG34550.083.6730658
TCGGGAG48050.083.632893
AAGCGGC8700.081.16549
ATTCGGA6000.078.2881552
ATCCGGA7500.075.283152
GTCCGGA3100.073.466882
GTTCGGA4400.073.327742
CGGAGAG14600.072.7875755
TTGGGAG23750.070.51773
TCGGAGA8100.069.116874
TCGGGAA12100.068.618273
GAGAGCG52000.067.442287
AAGGGGC22250.067.0941249
AGAGCGT107650.066.608378
CCGGAGA8500.066.4224854
GGAGAGC53850.066.366336
GAGCGTC107500.066.172299
Produced by FastQC (version 0.11.2)