FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n02_30245-1.3420000003577e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n02_30245-1.3420000003577e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2174707
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT27000.1241546562364493Illumina Single End PCR Primer 1 (96% over 32bp)
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG26720.12286712646807132No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC25380.11670537686226236No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC25240.11606161197807337No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG24110.1108655096985479No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA22660.10419794482659044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA8800.077.652542
CGAACGG200.00208073271.1946261
CGGGAGA8000.068.207754
AGAGCGG6750.065.994818
GAGCGGC6400.065.15969
GATCGGG11850.060.079851
TCGGGAG10500.056.0361373
GAGAGCG9100.055.722187
AAGCGGC2250.050.5480549
TTGTGTT282050.049.9956781
GAGCGTC20200.047.8579989
AGAGCGT21050.046.376818
GGTCCGG857.2759576E-944.671141
GGTTCGG2100.042.942791
GGCGCCG4650.042.9010356-57
AAGAGCG22050.042.3392687
AGCGGCG5900.041.7668810-11
TCGGAGA3300.041.6974874
TCGGAAG23600.041.6192443
GATCCGG2400.041.53021
Produced by FastQC (version 0.11.2)