##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n02_30245-1.3420000003577e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2174707 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.959034481426695 33.0 33.0 33.0 33.0 33.0 2 31.883254617748506 33.0 33.0 33.0 33.0 33.0 3 31.83333662879643 33.0 33.0 33.0 33.0 33.0 4 35.53122834478392 37.0 37.0 37.0 33.0 37.0 5 35.53625844768973 37.0 37.0 37.0 33.0 37.0 6 35.603544753385165 37.0 37.0 37.0 33.0 37.0 7 35.4712929144018 37.0 37.0 37.0 33.0 37.0 8 35.53890202220345 37.0 37.0 37.0 33.0 37.0 9 35.429334618410664 37.0 37.0 37.0 33.0 37.0 10-11 35.34935510852726 37.0 37.0 37.0 33.0 37.0 12-13 35.33853549006832 37.0 37.0 37.0 33.0 37.0 14-15 37.10791752636102 40.0 37.0 40.0 33.0 40.0 16-17 37.060957866967826 40.0 37.0 40.0 33.0 40.0 18-19 37.07778082288786 40.0 37.0 40.0 33.0 40.0 20-21 37.0936395109778 40.0 37.0 40.0 33.0 40.0 22-23 37.19050681310172 40.0 37.0 40.0 33.0 40.0 24-25 36.83931168658582 40.0 37.0 40.0 33.0 40.0 26-27 36.79242583023829 40.0 37.0 40.0 33.0 40.0 28-29 36.76639335781786 40.0 37.0 40.0 33.0 40.0 30-31 36.74875810856359 40.0 37.0 40.0 33.0 40.0 32-33 36.70822046372224 40.0 37.0 40.0 33.0 40.0 34-35 36.65064144273229 40.0 37.0 40.0 33.0 40.0 36-37 36.539398640828395 40.0 37.0 40.0 33.0 40.0 38-39 36.497490466531815 40.0 37.0 40.0 33.0 40.0 40-41 36.331409702548434 40.0 37.0 40.0 33.0 40.0 42-43 36.276263882904686 40.0 37.0 40.0 33.0 40.0 44-45 36.1911068939402 40.0 37.0 40.0 33.0 40.0 46-47 36.10602485760151 40.0 37.0 40.0 33.0 40.0 48-49 35.99101580120909 38.5 37.0 40.0 27.0 40.0 50-51 34.910253427243305 37.0 35.0 38.5 27.0 38.5 52-53 35.341014904536564 37.0 37.0 38.5 30.0 40.0 54-55 36.10546409240417 37.0 37.0 40.0 33.0 40.0 56-57 36.20220723987185 40.0 37.0 40.0 33.0 40.0 58-59 36.01453598116896 37.0 37.0 40.0 27.0 40.0 60-61 35.81011648925579 37.0 37.0 40.0 27.0 40.0 62-63 35.57298845315714 37.0 37.0 40.0 27.0 40.0 64-65 35.3608444723818 37.0 37.0 40.0 27.0 40.0 66-67 35.16096329298614 37.0 37.0 40.0 27.0 40.0 68-69 35.0094008066374 37.0 35.0 40.0 27.0 40.0 70-71 34.680450515862596 37.0 33.0 37.0 27.0 40.0 72-73 34.458903429289556 37.0 33.0 37.0 27.0 40.0 74-75 34.28340530471461 37.0 33.0 37.0 27.0 40.0 76-77 34.00775391811403 37.0 33.0 37.0 27.0 38.5 78-79 33.72139557190923 37.0 33.0 37.0 27.0 37.0 80-81 33.47735304112232 37.0 33.0 37.0 27.0 37.0 82-83 33.21907985765439 37.0 33.0 37.0 27.0 37.0 84-85 33.022034232657546 37.0 33.0 37.0 27.0 37.0 86-87 32.84912933098574 37.0 33.0 37.0 27.0 37.0 88-89 32.686164389041835 37.0 33.0 37.0 24.5 37.0 90-91 32.523510063654555 37.0 33.0 37.0 22.0 37.0 92-93 32.376559922785006 35.0 33.0 37.0 22.0 37.0 94-95 32.25848263697132 33.0 33.0 37.0 22.0 37.0 96-97 32.05957607162712 33.0 33.0 37.0 22.0 37.0 98-99 31.95101064189337 33.0 33.0 37.0 22.0 37.0 100-101 30.73625872358897 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.07321099203045733 2306 2 0.09183056520356558 2306 3 0.09753032438102238 2306 4 0.09464204577566449 2306 5 0.06280758624168215 2306 6 0.1443630165956904 2306 7 0.3199427040598479 2306 8 0.24716684236822317 2306 9 0.2353951446306013 2306 10-11 0.3131033119726041 2306 12-13 0.37872502364573535 2306 14-15 0.6464779746638101 2306 16-17 0.6470917797198368 2306 18-19 0.6774234788275706 2306 20-21 0.6665760723285601 2306 22-23 0.4624653813969388 2306 24-25 0.6330280944937456 2306 26-27 0.6060038411531465 2306 28-29 0.5222559153050383 2306 30-31 0.5334343434173903 2306 32-33 0.5079474026021273 2306 34-35 0.407890769279895 2306 36-37 0.44021215917408796 2306 38-39 0.5022921460771883 2306 40-41 0.506029365736353 2306 42-43 0.6598899561392031 2306 44-45 0.5765791475557052 2306 46-47 0.6802124675828196 2306 48-49 0.6193120441645803 2306 50-51 0.4725535107985124 2306 52-53 0.4946911549509494 2306 54-55 0.7471442573546909 2306 56-57 0.5232086204198154 2306 58-59 0.6281812603667518 2306 60-61 0.5142717894501274 2306 62-63 0.4623362822483372 2306 64-65 0.6008971454524925 2306 66-67 0.6589911098636279 2306 68-69 0.5292216391857778 2306 70-71 0.6712051392728355 2306 72-73 0.48965499067151796 2306 74-75 0.5872507083311334 2306 76-77 0.59704117849477 2306 78-79 0.5675017410947589 2306 80-81 0.594469166689251 2306 82-83 0.6305909398158818 2306 84-85 0.6578449210412742 2306 86-87 0.5968429469435463 2306 88-89 0.596553357882577 2306 90-91 0.6628095134900889 2306 92-93 0.5356695153137281 2306 94-95 0.5286155148384566 2306 96-97 0.8434263788316585 2306 98-99 0.6040105897556103 2306 100-101 0.4836370581847653 2307 1 0.02930374137774905 2307 2 -0.053239857331647045 2307 3 -0.05924440546103682 2307 4 0.022791741957739475 2307 5 0.030752746814428633 2307 6 0.010765671929185316 2307 7 0.1108638753101232 2307 8 0.033263221139606 2307 9 0.12845826148635098 2307 10-11 0.11913664413516045 2307 12-13 0.12841798607534116 2307 14-15 0.4484762067830417 2307 16-17 0.3401736290870261 2307 18-19 0.3432577195169344 2307 20-21 0.3433146835469856 2307 22-23 0.28307904676403695 2307 24-25 0.31341661848892244 2307 26-27 0.2633815237073165 2307 28-29 0.30182137957861954 2307 30-31 0.2367473636836266 2307 32-33 0.3184566342179238 2307 34-35 0.21247906380180126 2307 36-37 0.12181858910864207 2307 38-39 0.12906588075458103 2307 40-41 0.2662842449701941 2307 42-43 0.23854250317536696 2307 44-45 0.16629909650960428 2307 46-47 -0.008751448917003302 2307 48-49 -0.00745788547143178 2307 50-51 0.15730466109179275 2307 52-53 0.23805135773039154 2307 54-55 0.1680716922884855 2307 56-57 0.1189168264306133 2307 58-59 0.22819970018270652 2307 60-61 0.1695180634365201 2307 62-63 0.21450661931645953 2307 64-65 0.32137529280711874 2307 66-67 0.06366018834115295 2307 68-69 -0.0389892888930774 2307 70-71 0.008723140045546529 2307 72-73 0.038263223610300656 2307 74-75 0.003727708319424039 2307 76-77 0.12597529859358048 2307 78-79 0.22047494189557426 2307 80-81 0.1693585277935341 2307 82-83 0.26751945186908443 2307 84-85 0.14045822346596282 2307 86-87 0.19265096211688615 2307 88-89 0.3337160263288368 2307 90-91 0.27588831882417253 2307 92-93 0.13924859578157367 2307 94-95 0.26103915058477156 2307 96-97 0.15638547170978967 2307 98-99 0.1647574901103539 2307 100-101 0.31369190931983937 2201 1 -0.018314663817147192 2201 2 -0.07228182304675457 2201 3 -0.043152451438050576 2201 4 -0.0960370674247315 2201 5 -0.07133024924622333 2201 6 -0.0060718795611123255 2201 7 0.11222285935487974 2201 8 0.09241005617279541 2201 9 0.09254186913479145 2201 10-11 0.08320631117957333 2201 12-13 0.08918148147943583 2201 14-15 0.2453017963356814 2201 16-17 0.25471862575926707 2201 18-19 0.2166923245931045 2201 20-21 0.15879460008324742 2201 22-23 0.33686144742598856 2201 24-25 0.41474637223490163 2201 26-27 0.42495582262984755 2201 28-29 0.27371937033285576 2201 30-31 0.3061027681410238 2201 32-33 0.24356366208114366 2201 34-35 0.24442013551946928 2201 36-37 0.30816758988211745 2201 38-39 0.35376818140394306 2201 40-41 0.3519398131172551 2201 42-43 0.22434647130084784 2201 44-45 0.18461525142119228 2201 46-47 0.3366198208471687 2201 48-49 0.37465946743271417 2201 50-51 0.22933891000802475 2201 52-53 0.2847546297250645 2201 54-55 0.3842918099291879 2201 56-57 0.40736636144401217 2201 58-59 0.5091365987130914 2201 60-61 0.4202276851514881 2201 62-63 0.4298552243566647 2201 64-65 0.4510912642423648 2201 66-67 0.4690349657411801 2201 68-69 0.5183173864077588 2201 70-71 0.4269920767821844 2201 72-73 0.32811041660222173 2201 74-75 0.3500698736999581 2201 76-77 0.4042369502403744 2201 78-79 0.44961080432430833 2201 80-81 0.2956464597274078 2201 82-83 0.33300476476818375 2201 84-85 0.5119357476146931 2201 86-87 0.27598879241926255 2201 88-89 0.2747962882323165 2201 90-91 0.39040543428834695 2201 92-93 0.4509380171115964 2201 94-95 0.4121283164869496 2201 96-97 0.493176524540182 2201 98-99 0.4675055878924468 2201 100-101 0.22485473557494018 1101 1 0.25002436591332255 1101 2 0.2501083092908942 1101 3 0.18745056118541825 1101 4 0.2480229960682152 1101 5 0.22302206923775003 1101 6 0.31900070980958617 1101 7 0.3654197734643603 1101 8 0.3335941942432328 1101 9 0.31100508884223643 1101 10-11 0.3558300306519584 1101 12-13 0.3207148540021265 1101 14-15 0.4790647410206006 1101 16-17 0.48614686083652714 1101 18-19 0.5989850489269841 1101 20-21 0.5248132870540232 1101 22-23 0.5775107979747531 1101 24-25 0.5766698492134807 1101 26-27 0.5448256768005351 1101 28-29 0.48486808308651064 1101 30-31 0.5418215016588448 1101 32-33 0.5117528273866441 1101 34-35 0.41173853817380746 1101 36-37 0.4335469947151154 1101 38-39 0.4677015455621003 1101 40-41 0.4147357179072628 1101 42-43 0.40732725535124814 1101 44-45 0.37550612765951286 1101 46-47 0.42592449638488716 1101 48-49 0.38434508517232047 1101 50-51 0.39591747807861566 1101 52-53 0.424178887005489 1101 54-55 0.551654358693213 1101 56-57 0.6414898326913416 1101 58-59 0.5437932310464859 1101 60-61 0.4649668629650492 1101 62-63 0.5073014416159367 1101 64-65 0.45693675130949174 1101 66-67 0.41949257502022874 1101 68-69 0.5289118718616237 1101 70-71 0.5969048378735096 1101 72-73 0.5896445558731287 1101 74-75 0.519941973900778 1101 76-77 0.6328164058801207 1101 78-79 0.5811826691566608 1101 80-81 0.6338476761463596 1101 82-83 0.5566795488975842 1101 84-85 0.5086724207173958 1101 86-87 0.7104060676439232 1101 88-89 0.7060942922860107 1101 90-91 0.5978017277190162 1101 92-93 0.6728583806277086 1101 94-95 0.565115885097363 1101 96-97 0.5119246802783621 1101 98-99 0.5744233994536145 1101 100-101 0.4095978787934378 2304 1 -0.004998374237828784 2304 2 -0.23732864767657702 2304 3 -0.08882103038649802 2304 4 -0.12228581941526784 2304 5 -0.08801987700240943 2304 6 0.0880503099901091 2304 7 0.02306414910555077 2304 8 -0.026418722639945713 2304 9 -0.0421414848749464 2304 10-11 -0.022789960213650318 2304 12-13 0.017169907262577055 2304 14-15 0.15455408730409204 2304 16-17 0.2513555686672575 2304 18-19 0.23561580362083845 2304 20-21 0.20558781548110971 2304 22-23 0.09281705141687979 2304 24-25 0.3139123477841963 2304 26-27 0.42771001102958195 2304 28-29 0.4528290055371116 2304 30-31 0.25683752300569296 2304 32-33 0.13015529967518802 2304 34-35 0.16633529600719754 2304 36-37 0.24686205203288125 2304 38-39 0.2509184931492783 2304 40-41 0.14162879887680901 2304 42-43 0.20771910122520865 2304 44-45 0.23622401273324556 2304 46-47 0.2918988152199873 2304 48-49 0.2876843453733855 2304 50-51 0.16911859778290506 2304 52-53 0.12632539671683674 2304 54-55 0.24194333598489237 2304 56-57 0.42169362436791147 2304 58-59 0.3974268999945778 2304 60-61 0.35876907337210895 2304 62-63 0.4483885933310958 2304 64-65 0.41743213475324126 2304 66-67 0.3686607342522734 2304 68-69 0.3881811277630902 2304 70-71 0.370455819965251 2304 72-73 0.36413248209502314 2304 74-75 0.4830003027410541 2304 76-77 0.38887643828804386 2304 78-79 0.42876019305668933 2304 80-81 0.5341533491966146 2304 82-83 0.4602594637951398 2304 84-85 0.3702863874408706 2304 86-87 0.3809798021817201 2304 88-89 0.5141713582008052 2304 90-91 0.47557337009781975 2304 92-93 0.4679080900159107 2304 94-95 0.3803963192377111 2304 96-97 0.4769803888572781 2304 98-99 0.34162912540812584 2304 100-101 0.4916083345258109 2202 1 6.483838180280088E-4 2202 2 -0.0683904292715738 2202 3 -0.049852140267667266 2202 4 0.04288745435366792 2202 5 -0.0013147965674065176 2202 6 0.14386426347847703 2202 7 0.05022657082020032 2202 8 0.1512657112182012 2202 9 0.11000833541055499 2202 10-11 0.2923974798677875 2202 12-13 0.2738872102602343 2202 14-15 0.29126081498473155 2202 16-17 0.33951220196072995 2202 18-19 0.3424912421999906 2202 20-21 0.39106388594368724 2202 22-23 0.28743862967587575 2202 24-25 0.18781355257966226 2202 26-27 0.17441568447302558 2202 28-29 0.21804774328572307 2202 30-31 0.3198440098590183 2202 32-33 0.2070431255415599 2202 34-35 0.2745909617085829 2202 36-37 0.20507670131780742 2202 38-39 0.2694226623280045 2202 40-41 0.31052204747582834 2202 42-43 0.22388994695405984 2202 44-45 0.30351020848532784 2202 46-47 0.2432581223225796 2202 48-49 0.28250787594792115 2202 50-51 0.27169653721038145 2202 52-53 0.3492469845298558 2202 54-55 0.39856542818451857 2202 56-57 0.4295755300334463 2202 58-59 0.39365569582506765 2202 60-61 0.36819131843613917 2202 62-63 0.3920628406221951 2202 64-65 0.42396264009526163 2202 66-67 0.47092421448697763 2202 68-69 0.5373199477421977 2202 70-71 0.4197825906719572 2202 72-73 0.5336740865781664 2202 74-75 0.3668512006546436 2202 76-77 0.31458318903013094 2202 78-79 0.43310430925998844 2202 80-81 0.40466122797251103 2202 82-83 0.5093702805983682 2202 84-85 0.646698982939931 2202 86-87 0.42990876306622994 2202 88-89 0.41201148971689605 2202 90-91 0.32826167498322434 2202 92-93 0.29480069289463273 2202 94-95 0.4113426289133031 2202 96-97 0.6075274061895968 2202 98-99 0.6036902579883403 2202 100-101 0.4127367307345118 1102 1 0.0582454221529396 1102 2 0.026868928374920387 1102 3 0.012878356687298975 1102 4 0.016082689690172458 1102 5 0.008610172699668794 1102 6 0.17097423790303168 1102 7 0.1384943641253713 1102 8 0.22805987832054342 1102 9 0.0834310650597061 1102 10-11 0.06049952731660113 1102 12-13 0.1339824470097497 1102 14-15 0.2279771253378513 1102 16-17 0.14988509481232626 1102 18-19 0.3035833562014929 1102 20-21 0.14586952667223585 1102 22-23 0.2453147371210136 1102 24-25 0.18507019725050355 1102 26-27 0.3133256899837562 1102 28-29 0.31692440180404446 1102 30-31 0.32145342614536077 1102 32-33 0.21291558742566252 1102 34-35 0.29017078960418274 1102 36-37 0.17131428450866792 1102 38-39 0.20714574099016403 1102 40-41 0.24474863746325326 1102 42-43 0.32210037212821874 1102 44-45 0.3985187571846538 1102 46-47 0.36525795276160267 1102 48-49 0.41213795041344525 1102 50-51 0.2087131117960226 1102 52-53 0.40099487561519 1102 54-55 0.42211603507151807 1102 56-57 0.3682333135888527 1102 58-59 0.40019368454926507 1102 60-61 0.34867767584241705 1102 62-63 0.3671875385917005 1102 64-65 0.3927740176369099 1102 66-67 0.34486763764052597 1102 68-69 0.1890932729742829 1102 70-71 0.22754000543223896 1102 72-73 0.42888045641891637 1102 74-75 0.37072637551440835 1102 76-77 0.47091063953951817 1102 78-79 0.464313420336552 1102 80-81 0.5043383556095336 1102 82-83 0.4820335183652489 1102 84-85 0.4063258074867093 1102 86-87 0.41800487588079704 1102 88-89 0.5301615794429182 1102 90-91 0.36720347317739765 1102 92-93 0.35549080607658823 1102 94-95 0.4468465782710922 1102 96-97 0.5593473606907438 1102 98-99 0.5037771579897523 1102 100-101 0.49106754420330745 2305 1 0.15017486326236806 2305 2 0.14425390842809804 2305 3 0.16004342836685836 2305 4 0.1404572801920949 2305 5 0.1734200264566752 2305 6 0.19747455032318584 2305 7 0.0816548924316507 2305 8 0.17982777164739616 2305 9 0.1685259674252464 2305 10-11 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-0.17352498177029219 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 822.0 3 190.0 4 157.0 5 240.0 6 770.0 7 1980.0 8 6102.0 9 11001.0 10 12193.0 11 12267.0 12 9383.0 13 7066.0 14 5622.0 15 4921.0 16 4398.0 17 4538.0 18 4874.0 19 5268.0 20 5973.0 21 6422.0 22 7513.0 23 8211.0 24 9321.0 25 10641.0 26 12872.0 27 15732.0 28 19147.0 29 23417.0 30 28820.0 31 36836.0 32 50656.0 33 73378.0 34 113500.0 35 193486.0 36 385121.0 37 747110.0 38 333713.0 39 1046.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.42574668141593 24.877719395280238 26.16620575221239 14.530328171091444 2 29.531664722509376 23.64941751326997 34.58644294800919 12.232474816211461 3 37.943345747244656 20.022708149589935 26.736956868339735 15.296989234825675 4 28.342680373611305 19.516914979100473 36.2027180443645 15.937686602923723 5 31.090986918404408 18.293867993425796 35.1008915353216 15.514253552848201 6 23.835439581816782 18.939522651207184 41.68267820694692 15.542359560029118 7 31.784924359419914 19.031552007070374 32.17218387577131 17.0113397577384 8 27.834616402573044 28.23617679888609 27.80101494804433 16.128191850496542 9 30.48155539640663 26.775604924452335 25.618870439478098 17.12396923966294 10-11 24.32412222171092 29.20390225944503 29.840527357231604 16.631448161612443 12-13 24.963768341090603 26.117818887505678 29.849961691220418 19.0684510801833 14-15 23.154307425395515 26.872736110785056 31.08004288299286 18.89291358082657 16-17 22.442601122041456 28.064223042215325 32.04418389181492 17.448991943928306 18-19 21.876515197713527 28.226383051186787 32.26032117876773 17.636780572331958 20-21 21.6374659242857 29.151047312635058 31.600863078849944 17.6106236842293 22-23 22.23921015112041 28.7939867798141 31.20273385135386 17.76406921771163 24-25 21.551344163706414 29.39006441721979 31.153306782237127 17.905284636836676 26-27 21.247824600121003 29.786491222483587 31.025851896053382 17.939832281342028 28-29 21.20424051643146 29.460312356549057 30.93024043213154 18.405206694887948 30-31 21.473657361668057 29.179800395003507 30.87985140783421 18.46669083549423 32-33 20.344961394112268 29.20883538213231 32.38824963571951 18.057953588035915 34-35 20.136872169448566 30.298324032139977 31.65837271174266 17.906431086668793 36-37 20.4607472748324 30.29799502684163 31.333043128619053 17.908214569706917 38-39 20.672284882241485 29.297873778633328 31.798524935801325 18.231316403323866 40-41 21.100279917139176 29.668125274974233 30.53193946148769 18.699655346398902 42-43 20.949508677889526 29.146355955571607 31.38764316527506 18.516492201263812 44-45 20.328038436640494 29.60489676820127 31.62826032491013 18.4388044702481 46-47 20.912944284314268 29.683957571798402 30.85897794157181 18.54412020231552 48-49 20.45029120895728 29.938791091172412 30.689960922187463 18.92095677768285 50-51 20.412677807053836 29.454610817517302 30.75295101966131 19.37976035576755 52-53 21.321609280798135 29.13973110973187 30.26637533426701 19.272284275202985 54-55 21.567051072360393 29.243315193444726 30.066600074282036 19.123033659912846 56-57 21.779731109303896 29.295128014350063 29.89118636051656 19.033954515829482 58-59 21.404576419375154 29.169056225965033 30.456027110636402 18.970340244023408 60-61 20.33797957223235 29.453948726464652 30.287227282843975 19.920844418459026 62-63 20.960537160636694 29.409503348721323 29.978377790005357 19.651581700636626 64-65 20.288702682820244 29.565631489555024 30.441402164751246 19.70426366287349 66-67 20.127138238732694 29.1197169211438 30.36000897652611 20.393135863597394 68-69 19.868287404135426 29.23457794678911 31.387535045403343 19.50959960367212 70-71 19.487794545923716 30.794843631780093 30.389674718774263 19.327687103521924 72-73 19.61379803313465 31.206774502230882 29.72011944153014 19.459308023104327 74-75 19.855982522540348 30.701821744762032 29.89184738030654 19.55034835239108 76-77 19.955470950617954 30.527741874486193 30.03448637667056 19.482300798225292 78-79 19.89419214450122 30.657089336172277 30.090253302834576 19.35846521649193 80-81 19.436945720668934 31.136415476012836 29.997811307996013 19.428827495322217 82-83 19.2749243319061 30.811817141591614 29.991441200354345 19.921817326147938 84-85 19.4247770565911 30.3680743147003 30.396307700540653 19.81084092816795 86-87 19.566247961589646 30.43789287483163 30.582372309407912 19.413486854170813 88-89 19.880475155778853 30.937722831639803 29.85129062701637 19.330511385564975 90-91 20.08015001314691 30.569053560967056 29.5941987535808 19.75659767230523 92-93 19.61320208240325 30.823898750336674 29.90509919456302 19.657799972697052 94-95 19.248520989780964 31.137191961748083 29.91155340175233 19.70273364671862 96-97 19.56331178827093 31.448050047703248 29.56752517133156 19.42111299269427 98-99 20.083233992611266 30.77242639867431 29.231684092813143 19.91265551590128 100-101 20.039165017874318 30.606384188571557 28.967418848159088 20.387031945395044 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 539.0 1 392.5 2 220.5 3 194.0 4 228.0 5 298.5 6 412.5 7 593.5 8 753.5 9 882.5 10 980.0 11 1008.0 12 972.5 13 883.0 14 835.0 15 829.5 16 825.0 17 826.0 18 859.5 19 1016.5 20 1298.5 21 1755.0 22 2450.5 23 3523.0 24 5086.0 25 7274.0 26 10891.5 27 15750.0 28 21194.0 29 26560.0 30 32023.0 31 37742.0 32 44499.0 33 54163.5 34 65950.0 35 81750.5 36 101095.0 37 120354.0 38 140027.0 39 151742.5 40 152821.5 41 142664.5 42 125982.0 43 115335.5 44 105273.0 45 95656.5 46 91935.0 47 83394.0 48 66980.5 49 53760.0 50 44068.5 51 35638.5 52 28403.0 53 22550.0 54 18400.5 55 14557.5 56 10866.5 57 7715.0 58 5536.0 59 4047.5 60 2933.0 61 2062.5 62 1465.0 63 1040.0 64 748.0 65 550.5 66 421.0 67 316.5 68 218.5 69 171.5 70 140.5 71 115.0 72 87.5 73 62.0 74 50.5 75 51.5 76 44.5 77 32.0 78 28.0 79 25.0 80 19.0 81 16.5 82 13.0 83 14.0 84 15.5 85 14.0 86 13.0 87 9.5 88 7.0 89 7.5 90 5.5 91 2.0 92 2.0 93 2.0 94 1.5 95 1.0 96 1.5 97 1.5 98 1.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2348362331109432 2 0.22798473541493178 3 0.20972940262757236 4 0.1987853995963594 5 0.2026020056954799 6 0.1304083722542853 7 0.10401401200253643 8 0.10033535552145645 9 0.09375975706152599 10-11 0.0738950120636941 12-13 0.1024735746010842 14-15 0.10502564253483343 16-17 0.09472540438780949 18-19 0.08872459600304777 20-21 0.0822409639551443 22-23 0.16358525539302535 24-25 0.6307286452841694 26-27 0.36161193209016207 28-29 0.17871373017146677 30-31 0.30463873983943585 32-33 0.34124137182618164 34-35 0.32482536727936223 36-37 0.3343668825271634 38-39 0.34080453136905337 40-41 0.3429657420516879 42-43 0.3609451756029663 44-45 0.3402757244998981 46-47 0.3629684366675603 48-49 0.35041502142587483 50-51 0.3580482336241158 52-53 0.35186349241530007 54-55 0.3353095382504402 56-57 0.3434715573178364 58-59 0.3353095382504402 60-61 0.31381238943912904 62-63 0.30192573068463935 64-65 0.3016268398455516 66-67 0.31408828867521005 68-69 0.31291571692186576 70-71 0.29776425054041766 72-73 0.28886650017680543 74-75 0.29560303985778313 76-77 0.29702851924420165 78-79 0.3003393100771736 80-81 0.3101797161640626 82-83 0.3378386145811827 84-85 0.324664426058315 86-87 0.33432089932114994 88-89 0.33528654664743346 90-91 0.3171461718751078 92-93 0.2962697963449789 94-95 0.29656868718406665 96-97 0.3042018993823076 98-99 0.3256990481936187 100-101 0.33160789016635345 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2174707.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.1195055881347 #Duplication Level Percentage of deduplicated Percentage of total 1 92.89740623779527 64.21022789576509 2 3.708427737484298 5.126493834484794 3 1.0297471141925367 2.135268342413899 4 0.5373734621014847 1.4857195206655547 5 0.29290138320526105 1.0122599396614211 6 0.2531918243073388 1.0500296229048676 7 0.20756049825014591 1.0042535313073888 8 0.12564526153438868 0.694763068539906 9 0.12154867949290384 0.7561246168296145 >10 0.7065484228733168 9.102551525365168 >50 0.06012505596878211 2.882727911675842 >100 0.05564354083843262 8.055596362813795 >500 0.003078898827827698 1.3523764415499624 >1k 8.018831280854785E-4 1.131607386022645 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT 2700 0.1241546562364493 Illumina Single End PCR Primer 1 (96% over 32bp) TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 2672 0.12286712646807132 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 2538 0.11670537686226236 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 2524 0.11606161197807337 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 2411 0.1108655096985479 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 2266 0.10419794482659044 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 6.437648841889964E-4 0.0 0.0 2 7.35731296215996E-4 0.0 0.0 3 8.736809142564952E-4 0.0 0.0 4 9.656473262834947E-4 0.0 0.0 5 0.0011955633563509934 0.0 0.0 6 0.0012875297683779929 0.0 0.0 7 0.0013794961804049924 0.0 0.0 8 0.0015634290044589914 0.0 0.0 9 0.0017933450345264902 0.0 0.0 10-11 0.0026210427427694858 0.0 0.0 12-13 0.00370164808408673 0.0 0.0 14-15 0.004713278616383725 0.0 4.598320601349975E-5 16-17 0.005862858766721218 0.0 4.598320601349975E-5 18-19 0.007104405329085711 0.0 4.598320601349975E-5 20-21 0.008782792348578452 0.0 9.19664120269995E-5 22-23 0.010392204559050943 0.0 9.19664120269995E-5 24-25 0.012783331271752931 0.0 9.19664120269995E-5 26-27 0.014852575542360419 0.0 9.19664120269995E-5 28-29 0.018071399963305403 0.0 9.19664120269995E-5 30-31 0.029061386200531843 0.0 1.3794961804049926E-4 32-33 0.03223422741546332 0.0 1.6094122104724914E-4 34-35 0.03561399305745555 0.0 1.83932824053999E-4 36-37 0.03841896862427904 0.0 1.83932824053999E-4 38-39 0.042672415180527765 0.0 1.83932824053999E-4 40-41 0.046420046470627994 0.0 1.83932824053999E-4 42-43 0.05131725791106572 0.0 1.83932824053999E-4 44-45 0.056651309808631686 0.0 1.83932824053999E-4 46-47 0.06168647086710992 0.0 1.83932824053999E-4 48-49 0.06771027085487838 0.0 1.83932824053999E-4 50-51 0.07465373496291684 0.0 1.83932824053999E-4 52-53 0.08205703113109031 0.0 1.83932824053999E-4 54-55 0.08957528531429751 0.0 1.83932824053999E-4 56-57 0.09739243033659246 0.0 1.83932824053999E-4 58-59 0.10684197917236668 0.0 1.83932824053999E-4 60-61 0.11626853640513413 0.0 2.2991603006749874E-4 62-63 0.12758040508445506 0.0 2.2991603006749874E-4 64-65 0.14001886231110675 0.0 2.2991603006749874E-4 66-67 0.15268723556782593 0.0 2.2991603006749874E-4 68-69 0.16726391187410533 0.0 2.2991603006749874E-4 70-71 0.1837029080239315 0.0 2.2991603006749874E-4 72-73 0.2018662743992639 0.0 2.758992360809985E-4 74-75 0.22225982626625104 0.0 2.758992360809985E-4 76-77 0.24644699262935188 0.0 2.758992360809985E-4 78-79 0.27320921852920876 0.0 2.758992360809985E-4 80-81 0.3027764199958891 0.0 2.758992360809985E-4 82-83 0.33606826114966293 0.0 3.218824420944982E-4 84-85 0.37457919618596897 0.0 3.448740451012481E-4 86-87 0.4166998128943348 0.0 3.67865648107998E-4 88-89 0.461809337993578 0.0 3.67865648107998E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 880 0.0 77.65254 2 CGAACGG 20 0.002080732 71.194626 1 CGGGAGA 800 0.0 68.20775 4 AGAGCGG 675 0.0 65.99481 8 GAGCGGC 640 0.0 65.1596 9 GATCGGG 1185 0.0 60.07985 1 TCGGGAG 1050 0.0 56.036137 3 GAGAGCG 910 0.0 55.72218 7 AAGCGGC 225 0.0 50.548054 9 TTGTGTT 28205 0.0 49.995678 1 GAGCGTC 2020 0.0 47.857998 9 AGAGCGT 2105 0.0 46.37681 8 GGTCCGG 85 7.2759576E-9 44.67114 1 GGTTCGG 210 0.0 42.94279 1 GGCGCCG 465 0.0 42.90103 56-57 AAGAGCG 2205 0.0 42.339268 7 AGCGGCG 590 0.0 41.76688 10-11 TCGGAGA 330 0.0 41.697487 4 TCGGAAG 2360 0.0 41.619244 3 GATCCGG 240 0.0 41.5302 1 >>END_MODULE