##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n02_30230-1.34200000035711.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2203502 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.851451462263253 33.0 33.0 33.0 27.0 33.0 2 31.695174771795077 33.0 33.0 33.0 27.0 33.0 3 31.638871668825352 33.0 33.0 33.0 27.0 33.0 4 35.29223663059984 37.0 37.0 37.0 33.0 37.0 5 35.313237746096895 37.0 37.0 37.0 33.0 37.0 6 35.48231950776537 37.0 37.0 37.0 33.0 37.0 7 35.25772111847414 37.0 37.0 37.0 33.0 37.0 8 35.332207549618744 37.0 37.0 37.0 33.0 37.0 9 35.133223387135565 37.0 37.0 37.0 33.0 37.0 10-11 35.05488740196287 37.0 37.0 37.0 33.0 37.0 12-13 35.01924323190993 37.0 37.0 37.0 33.0 37.0 14-15 36.665767038105706 40.0 37.0 40.0 33.0 40.0 16-17 36.53685837362526 40.0 37.0 40.0 33.0 40.0 18-19 36.61820479400518 40.0 37.0 40.0 33.0 40.0 20-21 36.598491401414655 40.0 37.0 40.0 33.0 40.0 22-23 36.78301290400462 40.0 37.0 40.0 33.0 40.0 24-25 36.341788208043376 40.0 37.0 40.0 33.0 40.0 26-27 36.231721142072935 40.0 37.0 40.0 30.0 40.0 28-29 36.18915866652265 40.0 37.0 40.0 33.0 40.0 30-31 36.18162656534916 40.0 37.0 40.0 33.0 40.0 32-33 36.18066650268527 40.0 37.0 40.0 33.0 40.0 34-35 36.094772094602135 40.0 37.0 40.0 33.0 40.0 36-37 35.89891794970006 37.0 37.0 40.0 27.0 40.0 38-39 35.85489960980294 37.0 37.0 40.0 27.0 40.0 40-41 35.641310060077096 37.0 37.0 40.0 27.0 40.0 42-43 35.54687674438235 37.0 37.0 40.0 27.0 40.0 44-45 35.3940699849603 37.0 37.0 40.0 27.0 40.0 46-47 35.26394121720788 37.0 37.0 40.0 27.0 40.0 48-49 35.114583286060096 37.0 37.0 40.0 27.0 40.0 50-51 33.97665579609186 37.0 33.0 38.5 24.5 38.5 52-53 34.44699914045914 37.0 33.0 38.5 27.0 40.0 54-55 35.16760252543452 37.0 37.0 40.0 27.0 40.0 56-57 35.23206309774169 37.0 37.0 40.0 27.0 40.0 58-59 34.95752193553716 37.0 35.0 40.0 27.0 40.0 60-61 34.746330386811536 37.0 33.0 40.0 27.0 40.0 62-63 34.4641434407593 37.0 33.0 40.0 27.0 40.0 64-65 34.21095419927007 37.0 33.0 37.0 27.0 40.0 66-67 33.94597327345289 37.0 33.0 37.0 27.0 40.0 68-69 33.811275415225396 37.0 33.0 37.0 27.0 40.0 70-71 33.45585935479069 37.0 33.0 37.0 27.0 37.0 72-73 33.294354395866215 37.0 33.0 37.0 27.0 37.0 74-75 33.17791451970545 37.0 33.0 37.0 27.0 37.0 76-77 32.871786592433324 37.0 33.0 37.0 22.0 37.0 78-79 32.655908866885525 37.0 33.0 37.0 22.0 37.0 80-81 32.48885229058108 37.0 33.0 37.0 22.0 37.0 82-83 32.10827650712366 35.0 33.0 37.0 22.0 37.0 84-85 31.955697793784623 33.0 33.0 37.0 22.0 37.0 86-87 31.83188896583711 33.0 33.0 37.0 22.0 37.0 88-89 31.759435888871444 33.0 33.0 37.0 22.0 37.0 90-91 31.655303920758865 33.0 33.0 37.0 15.0 37.0 92-93 31.47140256736776 33.0 33.0 37.0 15.0 37.0 94-95 31.26319445137785 33.0 33.0 37.0 15.0 37.0 96-97 31.05764596537693 33.0 33.0 37.0 15.0 37.0 98-99 30.953596593059594 33.0 33.0 37.0 15.0 37.0 100-101 29.58491460411654 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.10513823529188215 2306 2 0.1594482854731183 2306 3 0.08803108193365716 2306 4 0.2733563125519112 2306 5 0.16495101259119593 2306 6 0.16817842152996576 2306 7 0.2922585635886392 2306 8 0.17914836225638453 2306 9 0.2752085355130447 2306 10-11 0.34058605957770993 2306 12-13 0.3409717456484742 2306 14-15 0.5917410408951227 2306 16-17 0.7104807311018888 2306 18-19 0.6536759556478984 2306 20-21 0.7099071638670935 2306 22-23 0.5436783676660468 2306 24-25 0.6485943859604077 2306 26-27 0.5767530652452493 2306 28-29 0.5337066553932033 2306 30-31 0.7175163950529537 2306 32-33 0.4671954571732684 2306 34-35 0.683472972472174 2306 36-37 0.663691157081324 2306 38-39 0.5903758637348133 2306 40-41 0.676604587208999 2306 42-43 0.563044628323361 2306 44-45 0.6818830476542033 2306 46-47 0.7039845202196133 2306 48-49 0.6225949692584933 2306 50-51 0.5140084082281149 2306 52-53 0.6341747464584486 2306 54-55 0.7564388524132397 2306 56-57 0.6637045140760947 2306 58-59 0.6941382055289083 2306 60-61 0.6315647426823432 2306 62-63 0.6310733063348124 2306 64-65 0.765827563881551 2306 66-67 0.8025603381221913 2306 68-69 0.6236678385540273 2306 70-71 0.7320513407913509 2306 72-73 0.6012487525697949 2306 74-75 0.6642613986661772 2306 76-77 0.5934448582332621 2306 78-79 0.7124041084072772 2306 80-81 0.6578264274308978 2306 82-83 0.7299645285431708 2306 84-85 0.7304454528681248 2306 86-87 0.6497060463213842 2306 88-89 0.643704578473205 2306 90-91 0.8215522701771505 2306 92-93 0.7971316206922445 2306 94-95 0.6610414534444544 2306 96-97 0.825340671506698 2306 98-99 0.9106786981004298 2306 100-101 0.8284397694839676 2307 1 0.11116990560964624 2307 2 0.05471996827527548 2307 3 0.08365933553175608 2307 4 0.21971390212154773 2307 5 0.1921789175684694 2307 6 0.1962270871130798 2307 7 0.43978126516449834 2307 8 0.13396089022849367 2307 9 0.24620440152957457 2307 10-11 0.3035866867418662 2307 12-13 0.248109492097484 2307 14-15 0.4620178028436257 2307 16-17 0.45629301174165704 2307 18-19 0.4943015445344372 2307 20-21 0.5710709067121229 2307 22-23 0.5673357799808372 2307 24-25 0.6034632262072677 2307 26-27 0.49307817772257323 2307 28-29 0.5403202192167171 2307 30-31 0.5168153992458286 2307 32-33 0.46110305793175144 2307 34-35 0.3656262508035084 2307 36-37 0.34557281149914587 2307 38-39 0.21159594829892825 2307 40-41 0.44136134762639756 2307 42-43 0.4533652475964445 2307 44-45 0.44038546503342246 2307 46-47 0.3891672517109086 2307 48-49 0.5006038113898015 2307 50-51 0.36014303063407027 2307 52-53 0.2265044257508393 2307 54-55 0.3286834523237303 2307 56-57 0.3277175108018824 2307 58-59 0.42300969282074163 2307 60-61 0.5625361993520741 2307 62-63 0.5415268520206311 2307 64-65 0.6327531934012498 2307 66-67 0.3962823660118602 2307 68-69 0.45080166890759443 2307 70-71 0.4654395877939237 2307 72-73 0.4271304629318422 2307 74-75 0.4206452967416894 2307 76-77 0.3206788729083172 2307 78-79 0.426705070647877 2307 80-81 0.3259682548635894 2307 82-83 0.4817908129046984 2307 84-85 0.4930200206254156 2307 86-87 0.41832185380259546 2307 88-89 0.5051583479359039 2307 90-91 0.456168787266634 2307 92-93 0.4034514037464412 2307 94-95 0.35513182986073133 2307 96-97 0.30078042252547377 2307 98-99 0.340942386767896 2307 100-101 0.28478892325401617 2201 1 0.2719552869231201 2201 2 0.31072264975644615 2201 3 0.3356048595411636 2201 4 0.5191027907174757 2201 5 0.5198228922776451 2201 6 0.44029460665071696 2201 7 0.6516719994210121 2201 8 0.4291555151375306 2201 9 0.5927357461196792 2201 10-11 0.5783902227638791 2201 12-13 0.5415922668108806 2201 14-15 0.9231041916241267 2201 16-17 0.9344103983917265 2201 18-19 0.9073127595591615 2201 20-21 0.7459388431053853 2201 22-23 0.8520313975916025 2201 24-25 1.008842964281662 2201 26-27 0.8107084549935237 2201 28-29 0.9276299513850006 2201 30-31 0.946803395860357 2201 32-33 0.8408933711139923 2201 34-35 0.8954348228422759 2201 36-37 0.9057652413904123 2201 38-39 0.7953411495813967 2201 40-41 0.9317312839247407 2201 42-43 1.0449610980241104 2201 44-45 0.9063104507954733 2201 46-47 0.7348324541535121 2201 48-49 0.8970604396842958 2201 50-51 0.7552895694931294 2201 52-53 0.9255775415950467 2201 54-55 1.026166498612902 2201 56-57 0.9486613420768677 2201 58-59 1.0393242118415884 2201 60-61 0.9872368727545222 2201 62-63 1.083523046004835 2201 64-65 1.1032572301246262 2201 66-67 0.9695270768759414 2201 68-69 0.8598995891117838 2201 70-71 1.0352460966478176 2201 72-73 0.916143179533293 2201 74-75 0.7397473235406835 2201 76-77 0.7230175555812508 2201 78-79 0.8534550864678252 2201 80-81 0.81911953575203 2201 82-83 1.1411487735024792 2201 84-85 1.0613092976010634 2201 86-87 0.9854816524146557 2201 88-89 1.056524565228166 2201 90-91 1.0991850712663407 2201 92-93 1.1129447567316681 2201 94-95 1.0480498324341099 2201 96-97 1.2102827401478358 2201 98-99 1.0671460540954847 2201 100-101 0.9969283273165814 1101 1 0.1572159952348109 1101 2 0.025776090003169827 1101 3 0.05530413347637264 1101 4 0.08073994016025665 1101 5 0.08959877431761498 1101 6 0.1515028374386489 1101 7 0.264703721427054 1101 8 0.015513619969951264 1101 9 0.15749853016258442 1101 10-11 0.20401570109723366 1101 12-13 0.19490432991813122 1101 14-15 0.4293101509361392 1101 16-17 0.384860970088873 1101 18-19 0.42570199452786994 1101 20-21 0.4520152430538218 1101 22-23 0.4020696642595851 1101 24-25 0.39895108294631854 1101 26-27 0.36532092684681317 1101 28-29 0.40962069141959034 1101 30-31 0.47830647887674616 1101 32-33 0.4672846314197443 1101 34-35 0.4517091058768514 1101 36-37 0.33936442264222677 1101 38-39 0.40114811270931483 1101 40-41 0.575525578826614 1101 42-43 0.3959320904243171 1101 44-45 0.4593933026925541 1101 46-47 0.41795812464265225 1101 48-49 0.3542696616073471 1101 50-51 0.37788392098003243 1101 52-53 0.4829352244324099 1101 54-55 0.6301235322777003 1101 56-57 0.6446212253298853 1101 58-59 0.7137119526307458 1101 60-61 0.662017747854442 1101 62-63 0.7391524930656885 1101 64-65 0.736221714050977 1101 66-67 0.5459168567596109 1101 68-69 0.59967996625155 1101 70-71 0.6431892041764087 1101 72-73 0.6959072152057857 1101 74-75 0.5253279226540499 1101 76-77 0.6467264705036371 1101 78-79 0.6251025211056955 1101 80-81 0.6277301192446956 1101 82-83 0.5855022492494228 1101 84-85 0.5840659272751196 1101 86-87 0.5357859464462251 1101 88-89 0.5350011720169903 1101 90-91 0.632904151753749 1101 92-93 0.7081803098308193 1101 94-95 0.6594809041311009 1101 96-97 0.6886811387797529 1101 98-99 0.7063804994202094 1101 100-101 0.6611042959687197 2304 1 -0.016211110550337082 2304 2 -0.17554243663019165 2304 3 -0.1868173425641153 2304 4 -0.15436407052629164 2304 5 -0.20181594172571238 2304 6 -0.012023855309067244 2304 7 0.1539689191908593 2304 8 0.051391499223143455 2304 9 -0.07677791405277645 2304 10-11 0.009801650311281662 2304 12-13 -0.028477709955879504 2304 14-15 0.11548056499284343 2304 16-17 0.15782310837036562 2304 18-19 0.14661763219186952 2304 20-21 0.17209530375676252 2304 22-23 0.21987590597805706 2304 24-25 0.15694948803016473 2304 26-27 0.1797873467351465 2304 28-29 0.0897132153398843 2304 30-31 0.15856211294430977 2304 32-33 0.14779411514919616 2304 34-35 0.15430254340731864 2304 36-37 0.2293244613467067 2304 38-39 0.29999703293670876 2304 40-41 0.346530119117034 2304 42-43 0.23230485221747443 2304 44-45 0.21660571298541953 2304 46-47 0.2743055953857194 2304 48-49 0.27320626631425426 2304 50-51 0.13185815979704785 2304 52-53 0.17108492004760478 2304 54-55 0.17779119528356802 2304 56-57 0.27436381514336006 2304 58-59 0.3193819154758799 2304 60-61 0.24970775184035432 2304 62-63 0.4096780060616041 2304 64-65 0.3588946322144366 2304 66-67 0.26825145602656875 2304 68-69 0.34874916113930965 2304 70-71 0.44504448887612114 2304 72-73 0.33612310619461994 2304 74-75 0.4586878287158598 2304 76-77 0.5481141635737075 2304 78-79 0.6527771062797072 2304 80-81 0.5744718248658245 2304 82-83 0.5293231645280017 2304 84-85 0.33579791948671556 2304 86-87 0.4234060934359505 2304 88-89 0.3839921127053927 2304 90-91 0.4322587824936477 2304 92-93 0.520800311803054 2304 94-95 0.534995957913253 2304 96-97 0.5662503307671649 2304 98-99 0.5219338599173895 2304 100-101 0.37126391740953935 2202 1 0.12487650421779506 2202 2 0.03929204722190249 2202 3 0.07232678249213009 2202 4 0.03623795191634116 2202 5 0.0822945646096116 2202 6 0.12107235886190892 2202 7 0.1177395918453783 2202 8 0.16259706282914976 2202 9 0.04954984232900017 2202 10-11 0.12408885505050904 2202 12-13 0.18251345962098497 2202 14-15 0.18618138394565165 2202 16-17 0.15116168056214718 2202 18-19 0.2192918886932631 2202 20-21 0.2243142421272779 2202 22-23 0.27920122392647784 2202 24-25 0.3853652156040397 2202 26-27 0.37209195534590833 2202 28-29 0.41534120379554906 2202 30-31 0.40358187815676416 2202 32-33 0.3632276071301703 2202 34-35 0.3755173246973982 2202 36-37 0.25150510791058167 2202 38-39 0.28386595731495134 2202 40-41 0.3180885398838811 2202 42-43 0.2988949746736509 2202 44-45 0.4064759998437637 2202 46-47 0.35048460193677755 2202 48-49 0.4104084229490965 2202 50-51 0.30306424249202735 2202 52-53 0.2882849335130757 2202 54-55 0.3325692680397978 2202 56-57 0.3220111728133972 2202 58-59 0.385919229622111 2202 60-61 0.471535032433394 2202 62-63 0.44895184168976243 2202 64-65 0.5110617130815598 2202 66-67 0.43574129845736564 2202 68-69 0.5021525475010975 2202 70-71 0.4927127899878201 2202 72-73 0.44204771031860446 2202 74-75 0.4946317856078366 2202 76-77 0.5328149151991397 2202 78-79 0.7589179546509897 2202 80-81 0.6595532074824675 2202 82-83 0.650850504199532 2202 84-85 0.6797579722487654 2202 86-87 0.7218811025135778 2202 88-89 0.6880577358318902 2202 90-91 0.5307095331554166 2202 92-93 0.551651543213179 2202 94-95 0.5797087924911608 2202 96-97 0.6565112963292101 2202 98-99 0.6724871393905296 2202 100-101 0.5370967866893146 1102 1 0.021630245936783865 1102 2 -0.04249381777384187 1102 3 0.04943553747002838 1102 4 -0.06508851078703515 1102 5 -0.06949414302746248 1102 6 0.011826769185390162 1102 7 0.13232593987569885 1102 8 0.0798914496728429 1102 9 0.12613984504773867 1102 10-11 0.19093333969488668 1102 12-13 0.16445053502675222 1102 14-15 0.14623536315504992 1102 16-17 0.22155517789499868 1102 18-19 0.21565912173576862 1102 20-21 0.23215855020860943 1102 22-23 0.20205806178941543 1102 24-25 0.11752907088333586 1102 26-27 0.1251604234481647 1102 28-29 0.14646795962695336 1102 30-31 0.293163966990619 1102 32-33 0.2258118707157024 1102 34-35 0.0920180594236868 1102 36-37 -0.06240696464182349 1102 38-39 0.1609225535176293 1102 40-41 0.14568210460139142 1102 42-43 0.09480214301449763 1102 44-45 0.26056820006959924 1102 46-47 0.1985026518752946 1102 48-49 0.11862105919275479 1102 50-51 0.032986152060772156 1102 52-53 0.17683683292408858 1102 54-55 0.30206829837266724 1102 56-57 0.18145637881674048 1102 58-59 0.32474311000148504 1102 60-61 0.3444032003546269 1102 62-63 0.33365128300231817 1102 64-65 0.22211305909487322 1102 66-67 0.266988479345855 1102 68-69 0.3435930875695945 1102 70-71 0.3735880089939201 1102 72-73 0.3541140675273411 1102 74-75 0.3698636586463735 1102 76-77 0.41583462473504085 1102 78-79 0.3208002073477516 1102 80-81 0.33813553721407885 1102 82-83 0.329031695063712 1102 84-85 0.3341649836843814 1102 86-87 0.24634580789275162 1102 88-89 0.2367653109587522 1102 90-91 0.10610398240378771 1102 92-93 0.28712244116617214 1102 94-95 0.3122276800914996 1102 96-97 0.39103897586416636 1102 98-99 0.3576484086525227 1102 100-101 0.3665038755648631 2305 1 0.07388357349645602 2305 2 0.07580199145513333 2305 3 0.11059686607104524 2305 4 0.06812784132586103 2305 5 0.05921057341958402 2305 6 0.2307889929363327 2305 7 0.13569966203384354 2305 8 0.2156398333332561 2305 9 0.03509806478727029 2305 10-11 0.14922811366616173 2305 12-13 0.048410652376261964 2305 14-15 0.11577094785490516 2305 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60-61 -0.6980416079977729 1213 62-63 -0.7128709563645756 1213 64-65 -0.5838195041394627 1213 66-67 -0.4446546505660933 1213 68-69 -0.41621311238770176 1213 70-71 -0.34660477740821705 1213 72-73 -0.23049138987641982 1213 74-75 -0.2697752195378271 1213 76-77 -0.3171605502740249 1213 78-79 -0.2867173899779729 1213 80-81 -0.4685689998473066 1213 82-83 -0.4009686819611318 1213 84-85 -0.296241653065465 1213 86-87 -0.568137570719621 1213 88-89 -0.41801922706683214 1213 90-91 -0.32951917940692965 1213 92-93 -0.4249737366362716 1213 94-95 -0.41195276214628507 1213 96-97 -0.4324737615494634 1213 98-99 -0.474130598542164 1213 100-101 -0.2150049562421863 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 787.0 3 191.0 4 203.0 5 252.0 6 851.0 7 2118.0 8 8810.0 9 16376.0 10 17562.0 11 15906.0 12 11235.0 13 8179.0 14 6540.0 15 6301.0 16 5948.0 17 6051.0 18 6334.0 19 6564.0 20 7403.0 21 8096.0 22 8825.0 23 9975.0 24 11313.0 25 12891.0 26 15457.0 27 19484.0 28 23414.0 29 28815.0 30 35538.0 31 47235.0 32 65694.0 33 95216.0 34 148231.0 35 259194.0 36 483439.0 37 659365.0 38 143576.0 39 133.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.07960929493783 20.800805281086493 27.045190624279336 12.074394799696336 2 36.25229747326835 19.61556637349672 33.14333458715525 10.988801566079674 3 43.23379575629505 15.09747988807991 27.606409803421357 14.062314552203684 4 35.78909461933199 13.644229711210476 34.75554694682624 15.811128722631288 5 35.16928979723238 12.729257731274357 36.68050947223489 15.420942999258372 6 27.44818373277051 13.41277396225472 43.81551296003053 15.323529344944237 7 37.42763669532732 14.290458037459935 31.271531830768893 17.010373436443853 8 33.6751774138527 26.881554364746435 23.0933944313195 16.34987379008136 9 35.006456576964986 27.58641207359453 20.33565116043515 17.071480189005335 10-11 31.15953661858907 26.60544438417179 26.054975169508328 16.180043827730813 12-13 31.56055978210507 22.684639243254836 26.20156098677767 19.553239987862426 14-15 29.084152175384453 23.400606255800334 26.435155931662894 21.080085637152315 16-17 27.74584524415954 26.136586356262605 27.741780025331536 18.375788374246316 18-19 27.009470250079826 26.33588843717483 27.249508002091076 19.40513331065427 20-21 25.75822500898682 28.043907892343505 27.206129973774168 18.991737124895515 22-23 27.634916803823202 25.249279445246703 27.229719809767182 19.886083941162916 24-25 27.321815167252257 25.61955069516135 27.607474454501507 19.45115968308488 26-27 23.893355292508005 26.1088650679009 29.393541005729894 20.6042386338612 28-29 23.398583247346572 27.231467267175923 27.172793947986936 22.197155537490566 30-31 22.907316913277082 27.239889064009446 27.93608433466176 21.916709688051707 32-33 22.20631820939133 24.86175956775899 30.478929890172747 22.45299233267693 34-35 22.238533313177197 27.021844213701197 28.280525164512326 22.45909730860928 36-37 21.164584238390454 29.66592804898374 28.370956386948233 20.798531325677576 38-39 20.470266289772347 27.82321215424978 28.09777666051413 23.608744895463744 40-41 22.841899366285862 27.67433970083592 26.080627201661365 23.403133731216855 42-43 25.240813511702576 26.947563760293818 26.73702986875567 21.074592859247936 44-45 21.654489969993545 29.32995303436784 26.42047925620064 22.59507773943797 46-47 22.425895925531403 29.436650288804728 27.01497694094518 21.12247684471869 48-49 22.054062055223454 26.953213777398233 28.8068317677199 22.18589239965841 50-51 20.705664473231728 27.132245133320975 27.229558217273492 24.9325321761738 52-53 22.969205344538647 28.247316424097107 26.37117594561365 22.41230228575059 54-55 23.36259276580417 27.94294774969068 27.492444172028343 21.202015312476803 56-57 24.856654333432687 27.431832677278756 25.087387289125722 22.624125700162832 58-59 22.876328166498975 26.074423999174872 25.975381433412316 25.07386640091383 60-61 20.49677889321405 25.97020191672927 28.464089781237906 25.068929408818775 62-63 22.384860192497925 26.05962400856922 28.472380603805984 23.083135195126868 64-65 22.8034899431514 28.357062737400263 27.37379774863523 21.465649570813106 66-67 21.93756935947415 27.879213499132117 26.813760919671058 23.369456221722675 68-69 21.187490709497336 24.652560809683386 30.96768965202472 23.19225882879456 70-71 20.990050378579067 29.287903541886784 28.242776771501028 21.47926930803312 72-73 23.453875895864734 30.086626145697515 26.39431271121424 20.065185247223503 74-75 24.720750083359032 28.28824358818248 26.802393001710424 20.188613326748065 76-77 23.330284185300496 27.44504079477838 26.60165054578536 22.623024474135768 78-79 23.640317074603033 28.90360434960147 26.324448367488245 21.131630208307254 80-81 22.655333407694567 29.786864012274155 26.22194096476871 21.335861615262566 82-83 22.442646933257436 29.387038565532947 26.895187135335135 21.275127365874486 84-85 22.887692134317298 28.580343249245576 26.75739083219217 21.774573784244957 86-87 20.506029604762848 29.265691498808287 27.942215794367755 22.286063102061114 88-89 19.99678955900836 29.544686606313412 28.381322974917577 22.07720085976065 90-91 21.520552373494088 28.637437737326643 27.04699636668275 22.795013522496514 92-93 21.997050241488715 28.79212396269102 25.94544767592715 23.265378119893118 94-95 22.595242766575275 30.028550866017138 25.77414407717474 21.602062290232844 96-97 21.26426878731725 31.347685525248657 24.625384937732786 22.762660749701304 98-99 21.547585087082027 29.200612974412575 26.478973798326315 22.77282814017908 100-101 23.123629908989983 29.863248182395257 26.17917762208211 20.83394428653265 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 528.0 1 387.0 2 213.0 3 204.0 4 199.0 5 195.5 6 266.5 7 393.5 8 535.5 9 658.0 10 780.0 11 883.5 12 927.5 13 886.5 14 805.0 15 696.0 16 588.5 17 555.0 18 552.5 19 527.5 20 512.5 21 572.0 22 665.0 23 793.0 24 942.0 25 1096.0 26 1338.5 27 1701.0 28 2151.0 29 2821.5 30 3642.0 31 4679.5 32 6331.5 33 9074.0 34 13787.0 35 22994.5 36 34952.5 37 47857.5 38 64373.5 39 78849.0 40 88858.0 41 96371.0 42 130551.5 43 167333.0 44 158144.5 45 136485.0 46 133418.5 47 129426.5 48 114291.0 49 103549.0 50 93710.5 51 88198.5 52 86586.5 53 87355.5 54 74962.5 55 47327.0 56 31217.5 57 25365.5 58 21668.5 59 17861.5 60 13903.5 61 10810.5 62 8323.5 63 6426.0 64 5097.5 65 3903.5 66 3027.5 67 2375.5 68 1855.0 69 1364.5 70 981.5 71 820.0 72 592.0 73 375.0 74 279.5 75 215.0 76 156.5 77 124.5 78 106.0 79 82.0 80 65.0 81 56.5 82 49.5 83 39.5 84 25.5 85 17.0 86 15.5 87 14.0 88 14.0 89 12.5 90 9.5 91 6.5 92 4.5 93 6.0 94 7.0 95 4.0 96 1.0 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22759225995710466 2 0.22196485412765682 3 0.20117975840276073 4 0.1898795644387888 5 0.19446317725148424 6 0.12239607679049078 7 0.09575666371076585 8 0.09126381550822282 9 0.0849102927975559 10-11 0.06678006191961704 12-13 0.09412290072802294 14-15 0.09625586906660398 16-17 0.08615830618715117 18-19 0.0800997684594795 20-21 0.07558422910439835 22-23 0.15706815786870174 24-25 0.6276599703562783 26-27 0.35908748891537196 28-29 0.18314029213497424 30-31 0.3140455511272511 32-33 0.3489899260359192 34-35 0.33192617932727086 36-37 0.3407303465120522 38-39 0.34767383918870964 40-41 0.3502152482729764 42-43 0.36900352257452 44-45 0.34676619308718576 46-47 0.37034230057426765 48-49 0.35747641708516714 50-51 0.36355764596537693 52-53 0.35749910823770525 54-55 0.34104802264758555 56-57 0.348241118002162 58-59 0.3393008039021521 60-61 0.32003601539730847 62-63 0.3087358214333366 64-65 0.3081458514673461 66-67 0.3206032942107609 68-69 0.3194233542787799 70-71 0.3056498246881555 72-73 0.2964599079102266 74-75 0.3031311067564268 76-77 0.3046741051290173 78-79 0.30837276299272703 80-81 0.3184476347196417 82-83 0.343294446748857 84-85 0.3305874013275232 86-87 0.3405488172917474 88-89 0.3422733448846427 90-91 0.32502806895568964 92-93 0.30460603167140304 94-95 0.3050144724170888 96-97 0.3122529500767415 98-99 0.33244807583564706 100-101 0.3394596419699188 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2203502.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.77992840107238 #Duplication Level Percentage of deduplicated Percentage of total 1 87.79741198201735 41.071566463182265 2 5.6403617570504885 5.277114383019374 3 1.8917710148097073 2.654907378720665 4 1.0253759803247948 1.9186805977509325 5 0.6267890497345385 1.4660573434578958 6 0.4248407426624314 1.1924411714164176 7 0.2639324206098367 0.8642717817194917 8 0.26858967258953886 1.0051684522404882 9 0.22653949496175813 0.9537751218893529 >10 1.4945763154907477 13.86114064662384 >50 0.18415806169570098 6.006285944643823 >100 0.14196190980218032 12.782901614923842 >500 0.008462140574302656 2.702232191984746 >1k 0.004344082473610394 3.210919473957272 >5k 3.935000902705804E-4 1.0299683631860785 >10k+ 4.918751128167206E-4 4.002569071283401 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 31538 1.4312671374929544 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 21683 0.9840245209670787 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 12003 0.5447238078295368 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 11433 0.5188558939361072 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 10318 0.4682546237761527 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT 5937 0.269434745237354 Illumina Single End PCR Primer 1 (96% over 32bp) TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 5679 0.2577261105276964 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 5574 0.2529609684946961 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 5191 0.23557954565051448 No Hit GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC 2989 0.13564770987273894 No Hit TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 2921 0.13256171312755785 No Hit GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC 2821 0.12802348261993862 No Hit AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC 2592 0.11763093475749059 No Hit TGTGTTTGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAA 2485 0.112775028114338 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 2342 0.10628535848844249 No Hit GTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCCT 2290 0.10392547862448048 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0029044675248763104 0.0 0.0 2 0.0031313790502572724 0.0 0.0 3 0.0035398197959430034 0.0 0.0 4 0.0037667313213239654 0.0 0.0 5 0.004175172067009696 0.0 0.0 6 0.00462899511777162 0.0 0.0 7 0.005264347388838313 0.0 0.0 8 0.005672788134524044 0.0 0.0 9 0.006308140405590737 0.0 0.0 10-11 0.010369856709909952 0.0 0.0 12-13 0.014340808404076782 0.0 2.2691152538096175E-5 14-15 0.01797139281017217 0.0 4.538230507619235E-5 16-17 0.02309959328378191 0.0 4.538230507619235E-5 18-19 0.028091646842163066 0.0 4.538230507619235E-5 20-21 0.03199452507871561 0.0 4.538230507619235E-5 22-23 0.035352815654353845 0.0 4.538230507619235E-5 24-25 0.03907416467060161 0.0 4.538230507619235E-5 26-27 0.04277282253431129 0.0 4.538230507619235E-5 28-29 0.04815062568584008 0.0 4.538230507619235E-5 30-31 0.1014748341503661 0.0 4.538230507619235E-5 32-33 0.10937135523362357 0.0 4.538230507619235E-5 34-35 0.11620139214759051 0.0 4.538230507619235E-5 36-37 0.12316757597678604 0.0 4.538230507619235E-5 38-39 0.13106409706004352 0.0 4.538230507619235E-5 40-41 0.1407078368887344 0.0 4.538230507619235E-5 42-43 0.15114576705625862 0.0 4.538230507619235E-5 44-45 0.16353513634205913 0.0 4.538230507619235E-5 46-47 0.17714982786491684 0.0 4.538230507619235E-5 48-49 0.19260250274336033 0.0 4.538230507619235E-5 50-51 0.2088266768080991 0.0 4.538230507619235E-5 52-53 0.2295436990753809 0.0 4.538230507619235E-5 54-55 0.2540047615114486 0.0 4.538230507619235E-5 56-57 0.2811887622520878 0.0 4.538230507619235E-5 58-59 0.30991576136531757 0.0 4.538230507619235E-5 60-61 0.34454246013845236 0.0 4.538230507619235E-5 62-63 0.38479656474103496 0.0 4.538230507619235E-5 64-65 0.4271609465296605 0.0 4.538230507619235E-5 66-67 0.4756973218086482 0.0 4.538230507619235E-5 68-69 0.5282954133919552 0.0 4.538230507619235E-5 70-71 0.5867932046351672 0.0 4.538230507619235E-5 72-73 0.6512133866908221 0.0 4.538230507619235E-5 74-75 0.7199675788812536 0.0 4.538230507619235E-5 76-77 0.8003850234762664 0.0 4.538230507619235E-5 78-79 0.8881770926461605 0.0 4.538230507619235E-5 80-81 0.9894023241186076 0.0 4.538230507619235E-5 82-83 1.1013604707415743 0.0 4.538230507619235E-5 84-85 1.227795572683846 0.0 4.538230507619235E-5 86-87 1.368889159165728 0.0 4.538230507619235E-5 88-89 1.525321964763363 0.0 4.538230507619235E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 1090 0.0 75.65587 9 ATTCGGA 190 0.0 69.91948 2 AGAGCGG 1250 0.0 66.731735 8 CGGGAGA 1425 0.0 65.58516 4 AAGCGGC 445 0.0 62.836487 9 GTTCGGA 205 0.0 62.48901 2 TCGGGAG 1455 0.0 62.279396 3 ATCGGGA 1820 0.0 59.43704 2 GGTTCGG 275 0.0 55.214172 1 TTGTGTT 28930 0.0 51.697678 1 TCGGGAA 445 0.0 49.042408 3 TCGGAAA 500 0.0 48.389866 4 GATCCGG 345 0.0 48.137356 1 TCGGAGA 355 0.0 48.109226 4 CGGAGAG 810 0.0 45.679813 5 ATCCGGA 340 0.0 44.654457 2 GGAGAGC 2180 0.0 43.48829 6 TGTGTTT 53045 0.0 42.0781 1 CGGAAAG 680 0.0 41.855938 5 ACCGGAA 205 0.0 41.657448 3 >>END_MODULE