FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n02_30230-1.34200000035711.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n02_30230-1.34200000035711.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2203502
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA315381.4312671374929544No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC216830.9840245209670787No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT120030.5447238078295368No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT114330.5188558939361072No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC103180.4682546237761527No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT59370.269434745237354Illumina Single End PCR Primer 1 (96% over 32bp)
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT56790.2577261105276964No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT55740.2529609684946961No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC51910.23557954565051448No Hit
GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC29890.13564770987273894No Hit
TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT29210.13256171312755785No Hit
GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC28210.12802348261993862No Hit
AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC25920.11763093475749059No Hit
TGTGTTTGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAA24850.112775028114338No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG23420.10628535848844249No Hit
GTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCCT22900.10392547862448048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC10900.075.655879
ATTCGGA1900.069.919482
AGAGCGG12500.066.7317358
CGGGAGA14250.065.585164
AAGCGGC4450.062.8364879
GTTCGGA2050.062.489012
TCGGGAG14550.062.2793963
ATCGGGA18200.059.437042
GGTTCGG2750.055.2141721
TTGTGTT289300.051.6976781
TCGGGAA4450.049.0424083
TCGGAAA5000.048.3898664
GATCCGG3450.048.1373561
TCGGAGA3550.048.1092264
CGGAGAG8100.045.6798135
ATCCGGA3400.044.6544572
GGAGAGC21800.043.488296
TGTGTTT530450.042.07811
CGGAAAG6800.041.8559385
ACCGGAA2050.041.6574483
Produced by FastQC (version 0.11.2)