##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n01_53115004-1.3410000003588d.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4610883 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.66908529233989 33.0 33.0 33.0 33.0 33.0 2 32.67636350781401 33.0 33.0 33.0 33.0 33.0 3 32.717827800011406 33.0 33.0 33.0 33.0 33.0 4 36.64371423000757 37.0 37.0 37.0 37.0 37.0 5 36.6229592032589 37.0 37.0 37.0 37.0 37.0 6 36.502037679116995 37.0 37.0 37.0 37.0 37.0 7 36.35793708059823 37.0 37.0 37.0 37.0 37.0 8 36.31225580870302 37.0 37.0 37.0 37.0 37.0 9 36.3662714929006 37.0 37.0 37.0 37.0 37.0 10-11 36.28802498350099 37.0 37.0 37.0 35.0 37.0 12-13 36.296657386448544 37.0 37.0 37.0 35.0 37.0 14-15 38.12399696544024 40.0 37.0 40.0 35.0 40.0 16-17 38.162401540008716 40.0 37.0 40.0 37.0 40.0 18-19 38.11338413922019 40.0 37.0 40.0 37.0 40.0 20-21 38.12556250071841 40.0 37.0 40.0 37.0 40.0 22-23 37.84255434371247 40.0 37.0 40.0 33.0 40.0 24-25 38.00373258224076 40.0 37.0 40.0 35.0 40.0 26-27 37.98670807738995 40.0 37.0 40.0 35.0 40.0 28-29 37.760068841477874 40.0 37.0 40.0 33.0 40.0 30-31 37.690610562011656 40.0 37.0 40.0 33.0 40.0 32-33 37.52117652952808 40.0 37.0 40.0 33.0 40.0 34-35 37.463164864517275 40.0 37.0 40.0 33.0 40.0 36-37 37.52365230260668 40.0 37.0 40.0 33.0 40.0 38-39 37.32574574110859 40.0 37.0 40.0 33.0 40.0 40-41 37.02478418992631 38.5 37.0 40.0 33.0 40.0 42-43 36.83891458534082 37.0 37.0 40.0 33.0 40.0 44-45 36.7649069603371 37.0 37.0 40.0 33.0 40.0 46-47 36.96977010694047 37.0 37.0 40.0 33.0 40.0 48-49 36.63718869032244 37.0 37.0 40.0 33.0 40.0 50-51 36.615752123833985 37.0 37.0 40.0 33.0 40.0 52-53 36.55222101710236 37.0 37.0 40.0 33.0 40.0 54-55 36.22930412678006 37.0 37.0 40.0 33.0 40.0 56-57 36.13793160659249 37.0 37.0 40.0 33.0 40.0 58-59 36.0324554971358 37.0 37.0 40.0 33.0 40.0 60-61 35.75517704526443 37.0 35.0 40.0 27.0 40.0 62-63 35.558917131490865 37.0 33.0 40.0 27.0 40.0 64-65 35.17542084672285 37.0 33.0 38.5 27.0 40.0 66-67 34.32652270725586 37.0 33.0 37.0 27.0 40.0 68-69 34.133266556535915 37.0 33.0 37.0 27.0 40.0 70-71 33.65278570286863 37.0 33.0 37.0 27.0 38.5 72-73 32.43188755819655 37.0 33.0 37.0 22.0 37.0 74-75 31.32142151514146 35.0 30.0 37.0 15.0 37.0 76-77 28.745204985682786 33.0 27.0 33.0 15.0 37.0 78-79 30.61014332829525 33.0 27.0 37.0 15.0 37.0 80-81 30.80335209112875 33.0 33.0 37.0 15.0 37.0 82-83 30.70639235478324 33.0 33.0 37.0 15.0 37.0 84-85 30.535586567692132 33.0 33.0 37.0 15.0 37.0 86-87 30.361965050945773 33.0 27.0 37.0 10.5 37.0 88-89 30.231133602826183 33.0 27.0 37.0 6.0 37.0 90-91 30.085072316951006 33.0 27.0 37.0 6.0 37.0 92-93 29.92428933894007 33.0 27.0 37.0 6.0 37.0 94-95 29.840157514298237 33.0 27.0 37.0 6.0 37.0 96-97 29.704842109418088 33.0 27.0 37.0 6.0 37.0 98-99 29.42124339307677 33.0 27.0 37.0 6.0 37.0 100-101 28.021237580741044 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.12380060954138372 2306 2 0.14738642703633076 2306 3 0.14172663586935386 2306 4 0.16771504946004967 2306 5 0.19711262721640566 2306 6 0.21959365819206056 2306 7 0.2507063210429834 2306 8 0.27857020247664366 2306 9 0.24596467187161863 2306 10-11 0.2507999721724019 2306 12-13 0.25129590904651167 2306 14-15 0.46128765185726905 2306 16-17 0.49249687297058387 2306 18-19 0.5305532854877484 2306 20-21 0.5884167599778749 2306 22-23 0.5492588148533315 2306 24-25 0.5084087569686417 2306 26-27 0.5166664797148428 2306 28-29 0.5869230032788479 2306 30-31 0.6572239317810897 2306 32-33 0.5396363600809124 2306 34-35 0.6255519890787724 2306 36-37 0.6693582971563501 2306 38-39 0.656086942640421 2306 40-41 0.6813263134017404 2306 42-43 0.7856818004409263 2306 44-45 0.7960570991540763 2306 46-47 0.7461720216545302 2306 48-49 0.7818681177857485 2306 50-51 0.7421843064978688 2306 52-53 0.8135890856998955 2306 54-55 0.7308576014991175 2306 56-57 0.824892494395435 2306 58-59 0.6263866824394597 2306 60-61 0.6905551644282184 2306 62-63 0.7934589556286795 2306 64-65 0.5879676064357824 2306 66-67 0.7329842297641278 2306 68-69 0.8696511818458887 2306 70-71 0.8164345161086644 2306 72-73 0.21449537422726195 2306 74-75 0.22894729729674523 2306 76-77 0.16428861555056784 2306 78-79 0.5077235499316792 2306 80-81 0.6993049796841042 2306 82-83 0.7752261156742257 2306 84-85 0.7249853560696238 2306 86-87 0.5873081976462124 2306 88-89 0.7012312040293338 2306 90-91 0.7221497689999978 2306 92-93 0.5894526251323455 2306 94-95 0.6882935115435522 2306 96-97 0.6844913054852491 2306 98-99 0.68275183458546 2306 100-101 0.4033154324815129 2307 1 0.028760722468973654 2307 2 0.041562182208863874 2307 3 0.11403598032833884 2307 4 0.1496134682393233 2307 5 0.2021751747738918 2307 6 0.2042069950253662 2307 7 0.2643115477041178 2307 8 0.21769963925560631 2307 9 0.23969279920017073 2307 10-11 0.2670516491956789 2307 12-13 0.2987316025076012 2307 14-15 0.5851534816398569 2307 16-17 0.5215472504538639 2307 18-19 0.5168617199066716 2307 20-21 0.5156956577826435 2307 22-23 0.5467081032129073 2307 24-25 0.5046216232548417 2307 26-27 0.5157754133212578 2307 28-29 0.5073637773908288 2307 30-31 0.6321662071342402 2307 32-33 0.6622788230703591 2307 34-35 0.5683725307324821 2307 36-37 0.5899227192780074 2307 38-39 0.6530721012818077 2307 40-41 0.6659325713719895 2307 42-43 0.7757715662268723 2307 44-45 0.6978595169455559 2307 46-47 0.6179379531290508 2307 48-49 0.6618213926476741 2307 50-51 0.551055932244644 2307 52-53 0.6740593566219104 2307 54-55 0.7239069703024299 2307 56-57 0.7016280198321638 2307 58-59 0.5988878524826831 2307 60-61 0.7157736721040848 2307 62-63 0.5822669946176688 2307 64-65 0.4142238174128323 2307 66-67 0.586413817067573 2307 68-69 0.5691098112874826 2307 70-71 0.5537274056924488 2307 72-73 0.23829143659228436 2307 74-75 0.07494988073361952 2307 76-77 0.12398330998912144 2307 78-79 0.6414927671591251 2307 80-81 0.7177451278389064 2307 82-83 0.6341335244935387 2307 84-85 0.5959857839346796 2307 86-87 0.65513310556587 2307 88-89 0.7276790549155194 2307 90-91 0.7885289641982567 2307 92-93 0.6741251110886353 2307 94-95 0.7635037624799601 2307 96-97 0.7296214364569842 2307 98-99 0.8221569872782766 2307 100-101 0.46276482690798915 2201 1 0.12049263389734222 2201 2 0.10855125469338134 2201 3 0.1104054229694853 2201 4 0.11930391161613585 2201 5 0.18178325328265998 2201 6 0.12695428040063206 2201 7 0.2522698054350698 2201 8 0.21032760510515658 2201 9 0.1678145419046757 2201 10-11 0.3104392231586601 2201 12-13 0.2598962442270434 2201 14-15 0.4376736173682687 2201 16-17 0.4758623090917311 2201 18-19 0.48420816864129534 2201 20-21 0.4653065000339467 2201 22-23 0.43784206646591173 2201 24-25 0.4446518508543278 2201 26-27 0.4881905206712389 2201 28-29 0.49144673608864053 2201 30-31 0.5401589998359881 2201 32-33 0.6177784601686724 2201 34-35 0.5278496051749286 2201 36-37 0.5645354178892674 2201 38-39 0.6347657732357348 2201 40-41 0.6538044043782634 2201 42-43 0.6826532645923606 2201 44-45 0.5464856103107607 2201 46-47 0.6004260341684287 2201 48-49 0.7144910744742532 2201 50-51 0.6732434442159771 2201 52-53 0.6454480997578713 2201 54-55 0.6877672623471298 2201 56-57 0.5943285660179427 2201 58-59 0.6626504532640354 2201 60-61 0.6897638867506473 2201 62-63 0.6186183738237858 2201 64-65 0.6626963387152642 2201 66-67 0.5801093824573016 2201 68-69 0.8112400005067926 2201 70-71 0.6519809176019606 2201 72-73 0.104197288383169 2201 74-75 0.011105374167446058 2201 76-77 0.10336893341333209 2201 78-79 0.29372409127936905 2201 80-81 0.32026457032086597 2201 82-83 0.3355771819919937 2201 84-85 0.25538296557459716 2201 86-87 0.3766842994543751 2201 88-89 0.36618533167162326 2201 90-91 0.33018593825245546 2201 92-93 0.4156221352126863 2201 94-95 0.4317017335375901 2201 96-97 0.44725789329181254 2201 98-99 0.44752254640413724 2201 100-101 0.3122144457097349 1101 1 0.05008611025370158 1101 2 0.0585729143639 1101 3 0.12239560760952628 1101 4 0.11996906418187336 1101 5 0.14851123134896227 1101 6 0.09630162539558995 1101 7 0.05088303291974228 1101 8 0.17293872336696126 1101 9 0.1541051468668897 1101 10-11 0.16758103241239297 1101 12-13 0.17093547937079734 1101 14-15 0.377798952816363 1101 16-17 0.3895268623835122 1101 18-19 0.36483210555283563 1101 20-21 0.3140792340379761 1101 22-23 0.37572822102323045 1101 24-25 0.34366574764357694 1101 26-27 0.43168479988861463 1101 28-29 0.43616931930537106 1101 30-31 0.43152627172782587 1101 32-33 0.40376181613047635 1101 34-35 0.43369480980691577 1101 36-37 0.38695864369928756 1101 38-39 0.535614167998105 1101 40-41 0.5173797585796152 1101 42-43 0.45764153515416695 1101 44-45 0.5431752099320946 1101 46-47 0.47540517030049756 1101 48-49 0.5083470632693547 1101 50-51 0.5167925300118057 1101 52-53 0.5550643014682279 1101 54-55 0.5637030223939732 1101 56-57 0.6146003568228835 1101 58-59 0.536304128280392 1101 60-61 0.4738688717964763 1101 62-63 0.6547515482734028 1101 64-65 0.4363219184401572 1101 66-67 0.3606756063809229 1101 68-69 0.6118868842130425 1101 70-71 0.5834686809261171 1101 72-73 0.1296672617134007 1101 74-75 -0.12498641854743298 1101 76-77 -0.08444117009636187 1101 78-79 0.3970848694220628 1101 80-81 0.23696853590897504 1101 82-83 0.24810615958634585 1101 84-85 0.2372746506139194 1101 86-87 0.23765481877438432 1101 88-89 0.2538986932663292 1101 90-91 0.30612778791911666 1101 92-93 0.3277370186587518 1101 94-95 0.2944792091410484 1101 96-97 0.286125030243209 1101 98-99 0.35999353420967495 1101 100-101 0.28861190965987404 2304 1 0.05381612473261299 2304 2 0.0821503354743669 2304 3 0.09438579070103259 2304 4 0.12096184526275522 2304 5 0.11866454352870193 2304 6 0.13052772235295862 2304 7 0.013515935993176242 2304 8 0.12724163044519798 2304 9 0.1892915757097029 2304 10-11 0.18173463211665108 2304 12-13 0.16396444746583683 2304 14-15 0.3035675161179654 2304 16-17 0.3284810946417096 2304 18-19 0.36578771382122 2304 20-21 0.36736142908227976 2304 22-23 0.49619010862168267 2304 24-25 0.3785985387878199 2304 26-27 0.3554411023233328 2304 28-29 0.3567285544901537 2304 30-31 0.37322821149144403 2304 32-33 0.4482958723825945 2304 34-35 0.4552482636951751 2304 36-37 0.38944265014805524 2304 38-39 0.47254237928754605 2304 40-41 0.3338873625762133 2304 42-43 0.45684509224319214 2304 44-45 0.4782683986816707 2304 46-47 0.342102027041399 2304 48-49 0.5034008200852185 2304 50-51 0.4879433468894874 2304 52-53 0.5460243252916896 2304 54-55 0.4774037709845871 2304 56-57 0.5446832254960654 2304 58-59 0.6155074799947613 2304 60-61 0.5629850419699167 2304 62-63 0.5079437892189418 2304 64-65 0.511079730378583 2304 66-67 0.3282081325918682 2304 68-69 0.5126681293995787 2304 70-71 0.617892348301325 2304 72-73 0.24214890635364128 2304 74-75 0.11911409180858712 2304 76-77 0.2042819129879554 2304 78-79 0.41668110285089455 2304 80-81 0.2419653012581655 2304 82-83 0.2860051768686205 2304 84-85 0.23411886366958612 2304 86-87 0.3716543874876095 2304 88-89 0.353987620491818 2304 90-91 0.358049786625255 2304 92-93 0.28584510733952584 2304 94-95 0.3091727388125527 2304 96-97 0.29225669593434844 2304 98-99 0.43741957366214734 2304 100-101 0.3500236550363809 2202 1 0.04662759669166405 2202 2 0.06730079503078912 2202 3 0.05526541187900591 2202 4 0.1119582649325892 2202 5 0.1262589416718498 2202 6 0.1200144423458056 2202 7 0.14964985002799125 2202 8 0.1312449686094297 2202 9 0.14358025985454503 2202 10-11 0.12688576228642745 2202 12-13 0.11020796952715983 2202 14-15 0.13430697910569478 2202 16-17 0.16515634267550894 2202 18-19 0.17882939244095297 2202 20-21 0.15708647760861538 2202 22-23 0.2862390261399739 2202 24-25 0.2804849368862605 2202 26-27 0.22955945067715788 2202 28-29 0.13617596908940754 2202 30-31 0.22735185023947935 2202 32-33 0.2119891636411566 2202 34-35 0.2690644634783439 2202 36-37 0.2262151743739622 2202 38-39 0.277030235597465 2202 40-41 0.21679391260583003 2202 42-43 0.25069098529526457 2202 44-45 0.2371077905098744 2202 46-47 0.2454629921285303 2202 48-49 0.2625334934853427 2202 50-51 0.17273771075684152 2202 52-53 0.18727237399311747 2202 54-55 0.29670089368075026 2202 56-57 0.2547514790326204 2202 58-59 0.2794934194681815 2202 60-61 0.292044022414089 2202 62-63 0.4125254196290271 2202 64-65 0.24742686220253063 2202 66-67 0.3616136562104941 2202 68-69 0.391652373711473 2202 70-71 0.3969751355194333 2202 72-73 0.12059856005635794 2202 74-75 0.05233989104930359 2202 76-77 0.07384579077239906 2202 78-79 0.27347747110703935 2202 80-81 0.23532846446682854 2202 82-83 0.33200984880829765 2202 84-85 0.2971004106255606 2202 86-87 0.17062030170125908 2202 88-89 0.2530971204750543 2202 90-91 0.19101885272165475 2202 92-93 0.1517545521834407 2202 94-95 0.2577454500650731 2202 96-97 0.3133216299348156 2202 98-99 0.21896127649846875 2202 100-101 0.18461679764230965 1102 1 0.07604003010101934 1102 2 0.04914257458040083 1102 3 0.09232568958427834 1102 4 0.1275420082991232 1102 5 0.17521369440278534 1102 6 0.12870036079512914 1102 7 0.13618141127157912 1102 8 0.09632133536717902 1102 9 0.105018214930233 1102 10-11 0.14639849832281726 1102 12-13 0.1387231651644143 1102 14-15 0.20785784021862241 1102 16-17 0.22341660086247117 1102 18-19 0.23137831488474347 1102 20-21 0.24337689851427058 1102 22-23 0.31318627442244207 1102 24-25 0.21533386734746074 1102 26-27 0.21965456791937754 1102 28-29 0.2549035314971775 1102 30-31 0.2661609986942537 1102 32-33 0.31884126410093216 1102 34-35 0.17760218628867364 1102 36-37 0.3490704692967128 1102 38-39 0.2580998914035888 1102 40-41 0.3190868299418881 1102 42-43 0.2491709695104234 1102 44-45 0.27990476580843193 1102 46-47 0.09003932558015038 1102 48-49 0.21653999436240667 1102 50-51 0.18861130383896096 1102 52-53 0.2462394732582709 1102 54-55 0.21537152322236608 1102 56-57 0.2924682729916199 1102 58-59 0.2551277947935091 1102 60-61 0.12374095688105768 1102 62-63 0.2849922760453225 1102 64-65 0.2419121722067814 1102 66-67 0.21275817700714583 1102 68-69 0.3788378917560138 1102 70-71 0.23775294191285212 1102 72-73 0.07771167151460645 1102 74-75 0.0070691215554781195 1102 76-77 -0.02118512808177897 1102 78-79 0.32110905323841266 1102 80-81 0.2571696377252941 1102 82-83 0.3272975306341479 1102 84-85 0.2355046204134652 1102 86-87 0.26618308813560176 1102 88-89 0.2603526704655543 1102 90-91 0.38231494497576435 1102 92-93 0.404080363473458 1102 94-95 0.42140221136146394 1102 96-97 0.2705629467087789 1102 98-99 0.18774416297350882 1102 100-101 0.3312744381938266 2305 1 0.05666952016786553 2305 2 -0.03531229544009307 2305 3 0.01558376017776908 2305 4 0.02993487410482487 2305 5 0.04674575137605075 2305 6 0.031870981525969455 2305 7 0.02623437751423552 2305 8 0.0026864942810789216 2305 9 -0.037178100544814185 2305 10-11 -0.016453204368666263 2305 12-13 -0.03028208710342284 2305 14-15 -0.08005569883359698 2305 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-0.9406315360574524 1213 58-59 -1.2529827052084244 1213 60-61 -0.5489890292028505 1213 62-63 -0.3051552431196569 1213 64-65 -0.5964503982553921 1213 66-67 -0.524359267301783 1213 68-69 -0.055435772694515606 1213 70-71 0.19763355558136908 1213 72-73 0.6581205607553855 1213 74-75 0.7453742530156013 1213 76-77 -1.8921226255097956 1213 78-79 -1.786153190138755 1213 80-81 -1.1764516184261318 1213 82-83 -0.7839243784493206 1213 84-85 -0.5496626968249743 1213 86-87 -0.3096119585947612 1213 88-89 -0.41867368658486015 1213 90-91 -1.484736075645479 1213 92-93 -0.6885498136293933 1213 94-95 -0.37768301922063685 1213 96-97 -0.41975240698235794 1213 98-99 -2.0646682649097485 1213 100-101 -0.6988972605157713 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 12.0 8 80.0 9 269.0 10 206.0 11 107.0 12 89.0 13 144.0 14 327.0 15 764.0 16 1492.0 17 2616.0 18 4033.0 19 6137.0 20 8652.0 21 12456.0 22 16844.0 23 23100.0 24 31707.0 25 43132.0 26 60149.0 27 90101.0 28 151531.0 29 245624.0 30 223852.0 31 119842.0 32 127655.0 33 180492.0 34 278107.0 35 480647.0 36 912313.0 37 1292917.0 38 295433.0 39 53.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.58296408867086 22.935280526367816 19.773380275111265 13.70837510985006 2 26.482228869671438 32.672410502352825 28.23750104107383 12.607859586901904 3 32.59140263485272 17.183972960921384 34.71330838325951 15.51131602096639 4 25.860144358466698 17.071198727011723 28.612719082223514 28.455937832298066 5 40.93959222092335 16.00991578522739 27.337334059160717 15.71315793468855 6 35.89201894734696 17.003792982819128 31.35863564527662 15.74555242455729 7 28.500679804714196 30.158258190459396 24.287603914477987 17.05345809034842 8 25.08346015286009 36.086341813487785 22.161199926348164 16.66899810730396 9 38.30049472085933 21.71950578663566 22.541756101813903 17.43824339069111 10-11 27.218987719250688 31.735158728007157 25.67531108520617 15.370542467535989 12-13 20.582868834451016 30.350770123640093 25.575480878608285 23.49088016330061 14-15 20.14410473483113 30.82332937349846 25.992356127053768 23.04020976461664 16-17 26.077434625862335 24.76788068576019 26.487974212314647 22.66671047606283 18-19 18.784399600770524 24.895052616831226 33.1770147229966 23.14353305940165 20-21 25.307386893139554 25.082538420515117 32.849152754472414 16.760921931872918 22-23 18.538932781421693 38.03826729066862 26.420514248572346 17.00228567933734 24-25 18.73681245002313 24.48060382360602 33.025789203499635 23.756794522871218 26-27 18.675002163360034 24.64202626698617 26.30854003452267 30.374431535131123 28-29 24.63130164005463 31.66220006016201 26.030545559277908 17.67595274050545 30-31 17.527955057632127 26.111690537365618 32.2644491304594 24.095905274542858 32-33 17.34148318228851 32.40784899551778 26.39521540668024 23.85545241551347 34-35 17.77901326058371 32.33225609931981 33.227952216527726 16.66077842356876 36-37 17.68589852376416 26.397777431977502 33.03185453110115 22.88446951315719 38-39 24.96230921871562 32.44990024128823 25.64651767327051 16.941272866725633 40-41 18.246583025311672 38.522541373365634 25.556971049108444 17.673904552214253 42-43 18.267603432920325 32.03928840089733 32.48914267322789 17.203965492954463 44-45 17.71118936112163 25.583448387741814 32.93565281285799 23.769709438278568 46-47 24.47924975136269 25.499923383597235 26.373057573566662 23.647769291473413 48-49 18.36727412678399 32.403915630607536 32.03026308041479 17.198547162193684 50-51 24.916804377121103 25.661438549542904 31.53908486224653 17.882672211089467 52-53 18.16651771882834 25.625274566660472 25.332041982143828 30.876165732367355 54-55 24.957672865535617 25.694268043723902 31.757845623151947 17.590213467588534 56-57 18.36450615141682 25.650594752641897 31.76764302694824 24.21725606899304 58-59 18.2784857465111 25.711714562972222 32.14969213817572 23.860107552340956 60-61 24.484699848418646 26.011781418752467 31.94548059922408 17.558038133604807 62-63 17.857377179494158 26.09107419609269 32.01726384834639 24.034284776066766 64-65 24.700565795268854 26.032666653869057 31.79647074504746 17.470296805814627 66-67 18.743945139260035 38.32871253877796 25.132627958441162 17.79471436352085 68-69 18.79904012767057 38.43992109104881 25.120651161559632 17.64038761972099 70-71 18.269975417490976 39.25412625674701 25.19933354616985 17.276564779592167 72-73 18.1251298970933 39.21176165841327 25.0079077228405 17.65520072165293 74-75 18.85425564019393 37.879025870184144 24.898861710161864 18.36785677946006 76-77 19.22748822157753 36.24178721343655 25.493841115177307 19.036883449808602 78-79 18.903573742619642 35.21720305741315 26.408919139764702 19.4703040602025 80-81 18.512814868272727 34.969861242973636 26.28848947271679 20.228834416036847 82-83 17.920114216734625 34.54550896216625 26.56709137924341 20.967285441855715 84-85 18.328106608273675 33.74919397674792 26.86367866734886 21.059020747629535 86-87 18.435528042151738 33.517154948594595 27.50270520510097 20.544611804152694 88-89 18.74546528003079 33.789655377010476 27.160029013530213 20.304850329428525 90-91 19.08923080260539 33.00182767765623 26.80149902108066 21.107442498657715 92-93 18.673312039069494 33.51515358609831 26.51911466630218 21.292419708530016 94-95 18.450515648323645 33.783125169558716 26.372103562910638 21.394255619207 96-97 18.93089088268188 34.04966692375532 26.09550680634065 20.92393538722215 98-99 19.434708363622793 32.878715704475255 26.00498177316714 21.681594158734814 100-101 19.458244777031194 33.202034627367155 25.503781138066262 21.835939457535385 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 89.0 1 64.5 2 113.0 3 300.5 4 307.0 5 178.0 6 150.5 7 148.0 8 158.5 9 158.5 10 153.5 11 146.0 12 151.5 13 162.5 14 166.5 15 179.5 16 194.0 17 233.0 18 281.5 19 365.0 20 516.5 21 741.0 22 1099.0 23 1681.5 24 2480.0 25 3534.5 26 5064.5 27 7159.5 28 9755.0 29 12857.0 30 16662.0 31 21571.5 32 29458.0 33 46680.0 34 76519.5 35 126742.0 36 193605.0 37 272161.5 38 353139.0 39 393468.5 40 394715.5 41 361841.0 42 317865.0 43 305578.0 44 285419.0 45 253743.5 46 240761.0 47 212600.5 48 161162.0 49 117847.0 50 89333.5 51 71802.5 52 58426.0 53 45403.5 54 34262.0 55 24561.5 56 16695.0 57 11573.0 58 8356.0 59 5985.0 60 4300.0 61 3238.0 62 2319.5 63 1503.0 64 969.0 65 648.0 66 432.5 67 286.5 68 185.5 69 133.5 70 97.0 71 68.5 72 50.0 73 36.0 74 33.0 75 30.5 76 18.0 77 12.5 78 10.0 79 7.0 80 5.5 81 4.5 82 2.5 83 1.0 84 2.5 85 2.5 86 1.5 87 1.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17389293981217913 2 0.005617145349383188 3 0.0016265864911341275 4 0.0 5 0.0011494544537347835 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.7350255905430694E-4 12-13 0.0 14-15 1.0843909940894184E-5 16-17 0.0 18-19 2.1687819881788367E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 1.0843909940894184E-5 38-39 1.6265864911341277E-4 40-41 1.301269192907302E-4 42-43 0.001225361823321043 44-45 4.554442175175557E-4 46-47 0.005454486700269775 48-49 0.003958027128426377 50-51 0.007894366436970966 52-53 0.004521910445352875 54-55 0.02073355580698968 56-57 0.012210242593446852 58-59 0.02041908241870375 60-61 0.01057281219237183 62-63 0.007016009731758538 64-65 0.01402117555357618 66-67 0.031805187856642644 68-69 0.03296548622031832 70-71 0.037118703727680794 72-73 0.03177265612681996 74-75 0.034700511810861395 76-77 0.03485232655003391 78-79 0.01826114434046581 80-81 0.0027218213951644404 82-83 0.0 84-85 0.006159340846427897 86-87 0.02713146267211725 88-89 0.02840020013520187 90-91 0.02619888641720035 92-93 0.03601262491370959 94-95 0.03234738335368735 96-97 0.03422337977346204 98-99 0.04080563310758482 100-101 0.02802066328727057 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4610883.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.93545027725076 #Duplication Level Percentage of deduplicated Percentage of total 1 87.82633510428104 29.804262279644927 2 5.425863891353896 3.682582685923409 3 1.7974828389768012 1.8299516851892634 4 0.9956136802194778 1.3514639416175491 5 0.6797168234347412 1.1533248232140247 6 0.5626561955354136 1.1456394808067467 7 0.2797550421409011 0.6645529325667918 8 0.30887305165074913 0.8385396869021363 9 0.20317835587550012 0.6205454093903936 >10 1.4589848167849702 10.194196419616656 >50 0.2152892519387279 5.155979162941814 >100 0.1925821968535118 13.63431719259361 >500 0.03669256193364591 8.664978172749114 >1k 0.0165861344290873 8.794533257646393 >5k 3.2504550083165646E-4 0.7669772632569686 >10k+ 6.500909068345205E-5 11.698155605940372 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 530261 11.500205058336983 TruSeq Adapter, Index 13 (97% over 38bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 8614 0.186818880461725 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT 7073 0.15339795002388912 TruSeq Adapter, Index 13 (97% over 38bp) TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 6762 0.14665303804065297 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 6340 0.13750077805053826 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5977 0.12962809943344908 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 4862 0.10544618026525505 No Hit GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT 4742 0.10284364187944045 No Hit ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG 4678 0.10145562140700598 TruSeq Adapter, Index 13 (97% over 37bp) TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 4624 0.10028447913338942 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.005660520989146764 0.0 0.0 2 0.00611596520666432 0.0 0.0 3 0.006614785063945453 0.0 0.0 4 0.006983478001935855 0.0 0.0 5 0.007807615157443813 0.0 0.0 6 0.008349810654488522 0.0 0.0 7 0.009607704207632248 0.0 2.168781988178837E-5 8 0.010561968282430936 0.0 2.168781988178837E-5 9 0.01158129581687499 0.0 2.168781988178837E-5 10-11 0.017534602374425896 0.0 3.253172982268256E-5 12-13 0.022446893577650964 0.0 4.337563976357674E-5 14-15 0.02710977485223546 0.0 4.337563976357674E-5 16-17 0.030981050701134685 0.0 6.50634596453651E-5 18-19 0.03578490280495081 0.0 1.1928300934983603E-4 20-21 0.04056706708888515 0.0 1.5181473917251858E-4 22-23 0.046346871087381745 0.0 1.5181473917251858E-4 24-25 0.0529508122413863 0.0 1.5181473917251858E-4 26-27 0.05913184090769599 0.0 1.5181473917251858E-4 28-29 0.06613700672951363 0.0 1.5181473917251858E-4 30-31 0.1021930072829868 0.0 1.6265864911341277E-4 32-33 0.1107705400462341 0.0 1.7350255905430696E-4 34-35 0.11833958918497824 0.0 1.7350255905430696E-4 36-37 0.12632070690147634 0.0 1.8434646899520113E-4 38-39 0.13509343004365976 0.0 1.9519037893609532E-4 40-41 0.14454931951211947 0.0 1.9519037893609532E-4 42-43 0.15453656056768303 0.0 1.9519037893609532E-4 44-45 0.16516359230975933 0.0 1.9519037893609532E-4 46-47 0.17637619518864392 0.0 1.9519037893609532E-4 48-49 0.18855390605226807 0.0 1.9519037893609532E-4 50-51 0.2014039393322277 0.0 1.9519037893609532E-4 52-53 0.21525161232674955 0.0 1.9519037893609532E-4 54-55 0.23034633496447426 0.0 1.9519037893609532E-4 56-57 0.24691582935416057 0.0 1.9519037893609532E-4 58-59 0.26465646601746345 0.0 1.9519037893609532E-4 60-61 0.28304773727722 0.0 2.060342888769895E-4 62-63 0.3042909568514317 0.0 2.168781988178837E-4 64-65 0.3263691574910923 0.0 2.168781988178837E-4 66-67 0.35108242824639013 0.0 2.168781988178837E-4 68-69 0.3767282752566049 0.0 2.168781988178837E-4 70-71 0.40435855778600327 0.0 2.494099286405662E-4 72-73 0.43377808545564916 0.0 2.710977485223546E-4 74-75 0.4665158495672087 0.0 2.819416584632488E-4 76-77 0.5041767487919342 0.0 2.819416584632488E-4 78-79 0.5455787969462682 0.0 2.92785568404143E-4 80-81 0.5913726286266643 0.0 3.0362947834503717E-4 82-83 0.6418835611313494 0.0 3.0362947834503717E-4 84-85 0.6971983457398507 0.0 3.0362947834503717E-4 86-87 0.7603532772356184 0.0 3.0362947834503717E-4 88-89 0.8293964518292918 0.0 3.1447338828593136E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGCGC 15 6.581623E-4 95.13617 1 GATCGGA 66200 0.0 87.2896 1 TCGGAAG 66455 0.0 86.66314 3 ATCGGAA 66840 0.0 86.22977 2 CGGAAGA 66595 0.0 86.217094 4 GAAGAGC 67290 0.0 85.29837 6 GGAAGAG 69225 0.0 82.98268 5 AGAGCAC 84720 0.0 67.74938 8 GAGCACA 84730 0.0 67.707756 9 AAGAGCA 85590 0.0 66.98304 7 TCGGACG 180 0.0 55.40583 3 GTCGCGG 45 1.5038842E-5 52.853428 1 GGCGCCG 90 2.237357E-10 47.568085 1 GCCGTCT 62350 0.0 46.038773 50-51 ATGCCGT 62180 0.0 46.033276 48-49 TCGTATG 62565 0.0 45.965687 44-45 TCTCGTA 61475 0.0 45.925484 42-43 CGTCTTC 62540 0.0 45.91559 52-53 AATCTCG 61040 0.0 45.842293 40-41 TTGTGTT 40190 0.0 45.816387 1 >>END_MODULE