FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n01_53115004-1.3410000003588d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n01_53115004-1.3410000003588d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4610883
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT53026111.500205058336983TruSeq Adapter, Index 13 (97% over 38bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC86140.186818880461725No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT70730.15339795002388912TruSeq Adapter, Index 13 (97% over 38bp)
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG67620.14665303804065297No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA63400.13750077805053826No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC59770.12962809943344908No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG48620.10544618026525505No Hit
GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT47420.10284364187944045No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG46780.10145562140700598TruSeq Adapter, Index 13 (97% over 37bp)
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA46240.10028447913338942No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGC156.581623E-495.136171
GATCGGA662000.087.28961
TCGGAAG664550.086.663143
ATCGGAA668400.086.229772
CGGAAGA665950.086.2170944
GAAGAGC672900.085.298376
GGAAGAG692250.082.982685
AGAGCAC847200.067.749388
GAGCACA847300.067.7077569
AAGAGCA855900.066.983047
TCGGACG1800.055.405833
GTCGCGG451.5038842E-552.8534281
GGCGCCG902.237357E-1047.5680851
GCCGTCT623500.046.03877350-51
ATGCCGT621800.046.03327648-49
TCGTATG625650.045.96568744-45
TCTCGTA614750.045.92548442-43
CGTCTTC625400.045.9155952-53
AATCTCG610400.045.84229340-41
TTGTGTT401900.045.8163871
Produced by FastQC (version 0.11.2)