##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n01_52370180-2.341000000358c0.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5192526 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.704972685741005 33.0 33.0 33.0 33.0 33.0 2 32.72268256336126 33.0 33.0 33.0 33.0 33.0 3 32.75983230512471 33.0 33.0 33.0 33.0 33.0 4 36.69514413601396 37.0 37.0 37.0 37.0 37.0 5 36.67351073446719 37.0 37.0 37.0 37.0 37.0 6 36.565526489419604 37.0 37.0 37.0 37.0 37.0 7 36.458635353968376 37.0 37.0 37.0 37.0 37.0 8 36.400595971979726 37.0 37.0 37.0 37.0 37.0 9 36.4836480356574 37.0 37.0 37.0 37.0 37.0 10-11 36.4013113270882 37.0 37.0 37.0 37.0 37.0 12-13 36.386466432715025 37.0 37.0 37.0 37.0 37.0 14-15 38.273247067034426 40.0 37.0 40.0 37.0 40.0 16-17 38.31239275450908 40.0 37.0 40.0 37.0 40.0 18-19 38.272060265081 40.0 37.0 40.0 37.0 40.0 20-21 38.28647974030366 40.0 37.0 40.0 37.0 40.0 22-23 38.09569773940468 40.0 37.0 40.0 37.0 40.0 24-25 38.162983199313786 40.0 37.0 40.0 37.0 40.0 26-27 38.120671422733366 40.0 37.0 40.0 37.0 40.0 28-29 37.93639454092286 40.0 37.0 40.0 35.0 40.0 30-31 37.94469108098833 40.0 37.0 40.0 37.0 40.0 32-33 37.82051933875728 40.0 37.0 40.0 33.0 40.0 34-35 37.65027734093195 40.0 37.0 40.0 33.0 40.0 36-37 37.32684997629285 40.0 37.0 40.0 33.0 40.0 38-39 37.55908993041152 40.0 37.0 40.0 33.0 40.0 40-41 37.32792324968618 40.0 37.0 40.0 33.0 40.0 42-43 37.15172750218294 40.0 37.0 40.0 33.0 40.0 44-45 37.24296363658073 40.0 37.0 40.0 33.0 40.0 46-47 37.2634474049817 40.0 37.0 40.0 33.0 40.0 48-49 36.945112166987705 37.0 37.0 40.0 33.0 40.0 50-51 36.89570076298125 37.0 37.0 40.0 33.0 40.0 52-53 36.86105346415213 37.0 37.0 40.0 33.0 40.0 54-55 36.5722248670493 37.0 37.0 40.0 33.0 40.0 56-57 36.4827823683502 37.0 37.0 40.0 33.0 40.0 58-59 36.22665346307366 37.0 37.0 40.0 33.0 40.0 60-61 35.97168073881575 37.0 37.0 40.0 33.0 40.0 62-63 35.802717020579195 37.0 37.0 40.0 33.0 40.0 64-65 35.52328558393353 37.0 35.0 40.0 30.0 40.0 66-67 34.91107593876275 37.0 33.0 37.0 27.0 40.0 68-69 34.7508977326257 37.0 33.0 37.0 27.0 40.0 70-71 34.35027759129179 37.0 33.0 37.0 27.0 40.0 72-73 33.51609582696361 37.0 33.0 37.0 24.5 40.0 74-75 32.56436443457385 37.0 33.0 37.0 22.0 38.5 76-77 30.0204695171483 33.0 30.0 35.0 18.5 37.0 78-79 32.04332140079799 35.0 33.0 37.0 18.5 37.0 80-81 32.26283460111706 37.0 33.0 37.0 15.0 37.0 82-83 32.1564699916765 37.0 33.0 37.0 15.0 37.0 84-85 31.99635610876094 37.0 33.0 37.0 15.0 37.0 86-87 31.81888371863713 37.0 33.0 37.0 15.0 37.0 88-89 31.67890993709035 37.0 33.0 37.0 15.0 37.0 90-91 31.52139806329328 35.0 33.0 37.0 15.0 37.0 92-93 31.36021832148746 33.0 33.0 37.0 15.0 37.0 94-95 31.280418528477277 33.0 33.0 37.0 15.0 37.0 96-97 31.157498874343624 33.0 33.0 37.0 15.0 37.0 98-99 30.883794804301413 33.0 33.0 37.0 15.0 37.0 100-101 29.47948946235416 33.0 30.0 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1499525938516726 2306 2 0.1290535574649283 2306 3 0.13563103485296324 2306 4 0.14348734882207026 2306 5 0.15832129270342676 2306 6 0.15448458417630917 2306 7 0.2226222221979839 2306 8 0.2580572022165697 2306 9 0.2291208258433528 2306 10-11 0.2603182822959482 2306 12-13 0.24335208822584065 2306 14-15 0.5727948961929314 2306 16-17 0.539735730895444 2306 18-19 0.5445882598597152 2306 20-21 0.5466928612608655 2306 22-23 0.512550407566394 2306 24-25 0.5046257904507812 2306 26-27 0.5325876463199677 2306 28-29 0.5034194431857557 2306 30-31 0.5511706198861503 2306 32-33 0.5507359277911092 2306 34-35 0.6991482065259547 2306 36-37 0.6639893273389177 2306 38-39 0.6637038241551565 2306 40-41 0.7570937318996229 2306 42-43 0.7981461524222624 2306 44-45 0.7886064198442426 2306 46-47 0.762554215201277 2306 48-49 0.7938316357789148 2306 50-51 0.6779910516855665 2306 52-53 0.7251948352086259 2306 54-55 0.6805944965570134 2306 56-57 0.7971684934220704 2306 58-59 0.7188715246610045 2306 60-61 0.6855247710569046 2306 62-63 0.7277287699206596 2306 64-65 0.6968110641587799 2306 66-67 0.5877283497426973 2306 68-69 0.6952503038364739 2306 70-71 0.5779944663563512 2306 72-73 0.28725235207691213 2306 74-75 0.2603319618237592 2306 76-77 -0.009454141792229365 2306 78-79 0.40580117677967564 2306 80-81 0.5876579894787639 2306 82-83 0.5955974975659046 2306 84-85 0.5871278218080889 2306 86-87 0.4926074345229843 2306 88-89 0.5312618986391726 2306 90-91 0.7115691865743798 2306 92-93 0.6764464201135034 2306 94-95 0.7550351026891029 2306 96-97 0.6591230696232593 2306 98-99 0.6692470077211574 2306 100-101 0.6450885144772442 2307 1 0.07773731310726362 2307 2 0.04355216582991517 2307 3 0.09595777426672214 2307 4 0.12202342250706977 2307 5 0.14486295770674218 2307 6 0.1594816834390329 2307 7 0.17345193400709746 2307 8 0.17261110277902247 2307 9 0.20341576620196378 2307 10-11 0.24327578163862995 2307 12-13 0.21763884564513347 2307 14-15 0.49560259115375516 2307 16-17 0.44848249767846937 2307 18-19 0.36139213224073785 2307 20-21 0.36806009888256597 2307 22-23 0.416596343375744 2307 24-25 0.41240199944947875 2307 26-27 0.4399412868026005 2307 28-29 0.4822797371628198 2307 30-31 0.47656630324035376 2307 32-33 0.48628350332384684 2307 34-35 0.47511408779869413 2307 36-37 0.5253038775380361 2307 38-39 0.48276484945757403 2307 40-41 0.4722738338055166 2307 42-43 0.4512423319379053 2307 44-45 0.46122292463401493 2307 46-47 0.5627164362376078 2307 48-49 0.5720225882623424 2307 50-51 0.5119698812744033 2307 52-53 0.4891900322056273 2307 54-55 0.6433593312700552 2307 56-57 0.5202234890453497 2307 58-59 0.4405678082146878 2307 60-61 0.522264233763579 2307 62-63 0.3507460704898193 2307 64-65 0.4211464603575905 2307 66-67 0.4690431125452861 2307 68-69 0.46785693556581265 2307 70-71 0.2103101861804646 2307 72-73 0.05436486657485773 2307 74-75 0.04143627013366569 2307 76-77 0.034486215745626936 2307 78-79 0.23153837166501745 2307 80-81 0.28932331704947245 2307 82-83 0.31234057912013924 2307 84-85 0.28867885589454545 2307 86-87 0.2462127075784899 2307 88-89 0.3656404686376433 2307 90-91 0.39963756627439295 2307 92-93 0.3675860022452042 2307 94-95 0.4852379340472872 2307 96-97 0.31166449173857913 2307 98-99 0.2433949151906205 2307 100-101 0.07374326372521622 2201 1 0.11061959646288244 2201 2 0.08656508101704219 2201 3 0.0864952194566655 2201 4 0.10272724158159008 2201 5 0.16140871027158 2201 6 0.10115273423444648 2201 7 0.17772476163329287 2201 8 0.1995400265066678 2201 9 0.15030060198213135 2201 10-11 0.19173160533279798 2201 12-13 0.1928087961002518 2201 14-15 0.37738806517521795 2201 16-17 0.34207687166711764 2201 18-19 0.3752691583586554 2201 20-21 0.3462307193744749 2201 22-23 0.3904025003481735 2201 24-25 0.3744016892660298 2201 26-27 0.39635111955020363 2201 28-29 0.3737770277881509 2201 30-31 0.5372629522792707 2201 32-33 0.5178332142754485 2201 34-35 0.46361858123500355 2201 36-37 0.5186824070995684 2201 38-39 0.5276804258361878 2201 40-41 0.6060087837419772 2201 42-43 0.623368497533292 2201 44-45 0.652301940656443 2201 46-47 0.6343278932922374 2201 48-49 0.7702836687887356 2201 50-51 0.657501161652867 2201 52-53 0.5172917399378036 2201 54-55 0.5269300833365023 2201 56-57 0.4706216760602828 2201 58-59 0.5202049408897338 2201 60-61 0.5276730491229955 2201 62-63 0.5349196191404673 2201 64-65 0.4887179638924266 2201 66-67 0.44023528132562717 2201 68-69 0.6378093504520521 2201 70-71 0.49004953072027035 2201 72-73 0.26351274399766567 2201 74-75 0.15777158451877682 2201 76-77 0.19028855523160715 2201 78-79 0.47535151283329213 2201 80-81 0.44417520445490766 2201 82-83 0.37435304119615864 2201 84-85 0.5125846736587292 2201 86-87 0.5380013949382452 2201 88-89 0.5709774146957898 2201 90-91 0.5336855717238933 2201 92-93 0.44790084181988377 2201 94-95 0.5271935899788396 2201 96-97 0.5876295998179089 2201 98-99 0.5109913888306039 2201 100-101 0.38497156651156317 1101 1 0.13601069486888662 1101 2 0.11511919645467117 1101 3 0.11850099226341371 1101 4 0.15028073718260515 1101 5 0.16255679571652593 1101 6 0.148198333856989 1101 7 0.09681387933748908 1101 8 0.21180169007415373 1101 9 0.12477350957096434 1101 10-11 0.19594001845852915 1101 12-13 0.1782338467510769 1101 14-15 0.331608042417038 1101 16-17 0.35675312267319725 1101 18-19 0.36719694276652604 1101 20-21 0.4223512822231328 1101 22-23 0.47458834031930053 1101 24-25 0.3469517156322226 1101 26-27 0.3821443716571835 1101 28-29 0.3507154049263548 1101 30-31 0.39162817481912526 1101 32-33 0.46468222392454805 1101 34-35 0.5519952568754221 1101 36-37 0.463151984222975 1101 38-39 0.4799039740770539 1101 40-41 0.4797170719752586 1101 42-43 0.5833654446066419 1101 44-45 0.4948028426574922 1101 46-47 0.4637192507344423 1101 48-49 0.57316544651718 1101 50-51 0.5387575797372932 1101 52-53 0.48732217669134315 1101 54-55 0.5044938478726024 1101 56-57 0.5404929905764888 1101 58-59 0.530284224469618 1101 60-61 0.4635819423908316 1101 62-63 0.6215172711623325 1101 64-65 0.45732046359176337 1101 66-67 0.5728460886859565 1101 68-69 0.5596997596367359 1101 70-71 0.6263118233756728 1101 72-73 0.5113784707329714 1101 74-75 0.4990277250644155 1101 76-77 0.3666385501433993 1101 78-79 0.9223767596115309 1101 80-81 0.8177738648676964 1101 82-83 0.7938126731797226 1101 84-85 0.7504627046135539 1101 86-87 0.7456360243777134 1101 88-89 0.7243657007087663 1101 90-91 0.701423075540049 1101 92-93 0.7430115335390504 1101 94-95 0.8308256475588855 1101 96-97 0.8093724508813445 1101 98-99 0.7014389663795626 1101 100-101 0.7471939120843594 2304 1 0.07622120420469258 2304 2 0.10580388949549757 2304 3 0.09029697564850636 2304 4 0.12303180922089041 2304 5 0.1572130713412605 2304 6 0.13102690182892474 2304 7 0.14427291336042458 2304 8 0.16064664443690901 2304 9 0.17797147706703242 2304 10-11 0.17191930358711005 2304 12-13 0.16115550394079037 2304 14-15 0.33590039633081403 2304 16-17 0.321657815445171 2304 18-19 0.3589770304115376 2304 20-21 0.34635956279471003 2304 22-23 0.32475502714969906 2304 24-25 0.35016144530381865 2304 26-27 0.320843273406048 2304 28-29 0.28969794313662334 2304 30-31 0.3052591920120662 2304 32-33 0.398946449435698 2304 34-35 0.3255068375774002 2304 36-37 0.3689456958278754 2304 38-39 0.4543607295985552 2304 40-41 0.41815787798673654 2304 42-43 0.43479337271622853 2304 44-45 0.41776849038370045 2304 46-47 0.3961485768626005 2304 48-49 0.4479718747141348 2304 50-51 0.5454016966331494 2304 52-53 0.3886178643417395 2304 54-55 0.290573481161573 2304 56-57 0.4482303176725111 2304 58-59 0.3570065430296623 2304 60-61 0.47668685427532864 2304 62-63 0.4734319853078688 2304 64-65 0.38358103274663335 2304 66-67 0.2626153484082394 2304 68-69 0.4732561710305916 2304 70-71 0.4460287582199385 2304 72-73 0.19234391010063945 2304 74-75 0.21519601155068102 2304 76-77 0.18053925721817876 2304 78-79 0.35879329521394965 2304 80-81 0.3051861526444455 2304 82-83 0.36048320456121274 2304 84-85 0.22413777955542002 2304 86-87 0.3035225630719047 2304 88-89 0.388557101550262 2304 90-91 0.4323707036133442 2304 92-93 0.29272487251822454 2304 94-95 0.3118804794605943 2304 96-97 0.26003163680437424 2304 98-99 0.3440541684873466 2304 100-101 0.251174064253302 2202 1 0.05572183996839897 2202 2 0.05507204962744083 2202 3 0.061057181923025894 2202 4 0.07782483123128259 2202 5 0.11117443634444868 2202 6 0.1508844029258043 2202 7 0.16971906618687882 2202 8 0.051552224478278674 2202 9 0.048724699496503376 2202 10-11 0.12134574387553698 2202 12-13 0.15855496198628316 2202 14-15 0.128503045637693 2202 16-17 0.1426228811746455 2202 18-19 0.17085885338418194 2202 20-21 0.16174370273672878 2202 22-23 0.2424933633152051 2202 24-25 0.2096104453432659 2202 26-27 0.20465303306012572 2202 28-29 0.18847031126393432 2202 30-31 0.19268301523925402 2202 32-33 0.2723457053868117 2202 34-35 0.25071016200963214 2202 36-37 0.22935123866272988 2202 38-39 0.21504608877557274 2202 40-41 0.27433824539188123 2202 42-43 0.3456505883598737 2202 44-45 0.3379391005338803 2202 46-47 0.2768241454411111 2202 48-49 0.27412552052523154 2202 50-51 0.278907191404528 2202 52-53 0.3652203823075979 2202 54-55 0.38979318133809215 2202 56-57 0.3937198567468698 2202 58-59 0.44004435907660167 2202 60-61 0.4398330175114751 2202 62-63 0.3154754288903945 2202 64-65 0.3181892685196175 2202 66-67 0.410520733674538 2202 68-69 0.43059153628670543 2202 70-71 0.2674901394882738 2202 72-73 0.1027423979304487 2202 74-75 0.05786477357418107 2202 76-77 -0.014135042475995618 2202 78-79 0.22637204492880159 2202 80-81 0.2655304419736595 2202 82-83 0.42007159177892817 2202 84-85 0.3109693127442341 2202 86-87 0.37926335385055054 2202 88-89 0.31980778011163125 2202 90-91 0.3190964473064284 2202 92-93 0.2512670096032785 2202 94-95 0.2984004386188239 2202 96-97 0.3381773303845641 2202 98-99 0.33329866588838186 2202 100-101 0.28667916500161894 1102 1 0.06370003288621007 1102 2 0.020821003740302046 1102 3 0.06457962570245002 1102 4 0.09126255338430411 1102 5 0.09954739770910237 1102 6 0.08758742011743692 1102 7 0.08358374240556543 1102 8 0.1324000032470849 1102 9 0.08359708687996914 1102 10-11 0.10641528272181944 1102 12-13 0.055966696418074946 1102 14-15 0.16265128892554515 1102 16-17 0.160452020021836 1102 18-19 0.17839350804341336 1102 20-21 0.18488398593526512 1102 22-23 0.20379982271286678 1102 24-25 0.19916256545290167 1102 26-27 0.14087291036296534 1102 28-29 0.2110143070587327 1102 30-31 0.14812568549474037 1102 32-33 0.17535614049719328 1102 34-35 0.1772606143249007 1102 36-37 0.20715088803474657 1102 38-39 0.2318712858866263 1102 40-41 0.21998104773901872 1102 42-43 0.15975762655556736 1102 44-45 0.2379755340336942 1102 46-47 0.18162182636964985 1102 48-49 0.18499679841649908 1102 50-51 0.23584955115090622 1102 52-53 0.29795418467509194 1102 54-55 0.22639766320813948 1102 56-57 0.17710572824297088 1102 58-59 0.167926686954182 1102 60-61 0.01875583377251644 1102 62-63 0.15106885689479554 1102 64-65 0.2720572486457371 1102 66-67 0.21877271125064368 1102 68-69 0.17776874824787825 1102 70-71 0.2431052336253856 1102 72-73 -0.02241992834366613 1102 74-75 -0.07334787360245087 1102 76-77 -0.094523765612756 1102 78-79 0.23166896502112166 1102 80-81 0.2208924542149262 1102 82-83 0.208577549965419 1102 84-85 0.14260742690490247 1102 86-87 0.19180119765331582 1102 88-89 0.2398244483569698 1102 90-91 0.2660147454255579 1102 92-93 0.25137676269277165 1102 94-95 0.25822628196382524 1102 96-97 0.3245967190840986 1102 98-99 0.19934396950680622 1102 100-101 0.23000777738959144 2305 1 0.06671009623912738 2305 2 0.012231241321707387 2305 3 0.05458903544735705 2305 4 0.042389947665341765 2305 5 0.04300402454581587 2305 6 0.043859193444909295 2305 7 0.051397692373321036 2305 8 0.012530545664709791 2305 9 -0.010454770376590261 2305 10-11 0.042437264917495554 2305 12-13 -0.005594286314980934 2305 14-15 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5938.0 20 8271.0 21 11635.0 22 15767.0 23 21152.0 24 28167.0 25 38268.0 26 53088.0 27 81792.0 28 144378.0 29 215868.0 30 152752.0 31 101718.0 32 128179.0 33 183353.0 34 285167.0 35 500116.0 36 994338.0 37 1643488.0 38 569750.0 39 357.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.94106743792673 24.28203671922265 23.006637147654736 12.770258695195883 2 26.814374864940966 29.833822980508746 32.01115756136061 11.340644593189673 3 34.45553924546195 18.1083124599657 32.754348548711945 14.68179974586041 4 25.382713538651515 18.398232382466645 33.57625941593744 22.642794662944393 5 36.86598811736271 17.443538214137835 31.683328679139905 14.007144989359551 6 30.539567832688753 17.97171550031719 37.31083869392277 14.177877973071295 7 29.904539717278254 26.57053618990064 28.34818737547005 15.176736717351055 8 25.319950251573125 34.04013769021089 26.216642920998375 14.42326913721761 9 36.499730574290815 23.20042306961968 24.420484365412904 15.8793619906766 10-11 26.338886487461906 30.91080438966516 28.02240610016592 14.727903022707014 12-13 22.389064590143605 29.206093527504724 27.427643116279054 20.977198766072622 14-15 21.32775480137313 30.04734690733314 28.007674493623046 20.61722379767069 16-17 24.686250969181472 26.51834579162435 28.6805304393276 20.11487279986658 18-19 19.06995518942233 26.863852723646538 33.34045254292518 20.725739544005958 20-21 23.61770552521066 26.975319911734672 33.00990693161671 16.397067631437956 22-23 18.92549021420403 36.05513963724014 28.58287084166743 16.4364993068884 24-25 18.96638553182016 26.963321897665992 33.03070605712903 21.039586513384815 26-27 19.018104098082514 27.082185048279005 28.203922329902632 25.695788523735846 28-29 23.010929555287735 31.643596969952583 28.188900739254848 17.156572735504838 30-31 18.0794087501921 27.761035765636993 32.39863411372423 21.760921370446678 32-33 17.894700960572944 32.29291485492802 28.34577044005172 21.466613744447308 34-35 22.647907781299505 32.28110942535483 28.57065135542894 16.500331437916728 36-37 18.049112531786083 32.317847736476516 33.238161248749165 16.394878482988233 38-39 18.430998632068903 27.755038841501218 32.61283120523723 21.201131321192648 40-41 22.836859559520235 27.63485744879172 32.32947586143145 17.1988071302566 42-43 18.313153887656384 32.1492046101135 32.615978169087114 16.92166333314299 44-45 17.91017210449332 27.822680277362384 32.88392744514428 21.383220173000016 46-47 22.55028866243059 27.71921049631141 28.315390476219825 21.41511036503818 48-49 18.51747209590521 32.337434314913054 32.08566696008228 17.059426629099452 50-51 22.981402228250396 27.688215973477405 31.77975850043735 17.550623297834854 52-53 18.258586829724035 27.55362140314771 27.603840160484317 26.583951606643936 54-55 22.80286836043951 27.549474209363726 31.97791375945241 17.66974367074435 56-57 18.4291271977609 27.436420958132214 31.895845136566965 22.238606707539923 58-59 18.457394385154338 27.507895404181802 32.32240890560948 21.71230130505438 60-61 22.61714233216002 27.91650973472394 32.08339588943119 17.382952043684845 62-63 18.133527959129353 28.04991962922746 32.01150729191905 21.805045119724138 64-65 22.731265009141275 27.799057982571096 32.03512206471853 17.434554943569093 66-67 18.498883821069235 36.263283762197254 27.479125103738756 17.758707312994755 68-69 18.394196497674617 36.49622591839453 27.44690649372441 17.66267109020644 70-71 18.03350791634349 37.04027469370912 27.474803753053084 17.451413636894312 72-73 17.887149762511754 36.90760261947449 27.434879404556657 17.7703682134571 74-75 18.411365607747246 35.90075417846851 27.403663923163286 18.284216290620954 76-77 18.92268157621377 34.87847077734932 27.75229021986727 18.446557426569633 78-79 18.931975111682974 34.16959247090367 28.22910311238796 18.669329305025386 80-81 18.537099157958718 34.083778889919884 28.095053484123472 19.284068467997926 82-83 18.089018716516776 33.84710062116203 28.208014750431676 19.855865911889513 84-85 18.172402835021764 33.12102769538924 28.84469974192057 19.861869727668427 86-87 18.117021970285894 32.90582538177084 29.419453666567108 19.557698981376166 88-89 18.39665966750785 33.25893355936122 28.942651846429467 19.40175492670147 90-91 18.78525499853454 32.62657269860619 28.726152053770264 19.86202024908901 92-93 18.39676661208525 33.13096563836611 28.632318871585454 19.839948877963188 94-95 18.161488818994933 33.33816552667038 28.488707934753638 20.011637719581046 96-97 18.583719512253857 33.46621869590806 28.16476796032993 19.785293831508156 98-99 18.911353643896803 32.54952652932732 28.061337431588974 20.4777823951869 100-101 18.794419690586405 32.71104831144013 27.779819730269633 20.714712267703824 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 402.0 1 285.0 2 248.0 3 565.0 4 649.0 5 434.0 6 359.0 7 344.5 8 331.5 9 329.0 10 326.0 11 339.0 12 347.5 13 364.5 14 396.5 15 442.5 16 568.0 17 735.0 18 1047.5 19 1529.0 20 2353.5 21 3733.0 22 5816.5 23 8913.5 24 13098.0 25 18691.5 26 26350.0 27 35991.5 28 47126.0 29 59062.5 30 71324.5 31 84709.0 32 101420.0 33 122792.5 34 149956.0 35 188924.0 36 245602.0 37 317155.0 38 381919.0 39 402661.0 40 401424.0 41 374257.5 42 322847.5 43 298070.5 44 270930.0 45 241105.5 46 233860.0 47 205328.0 48 145245.5 49 98496.0 50 72514.0 51 55925.5 52 43932.5 53 32922.0 54 24594.0 55 18720.0 56 13932.5 57 10441.5 58 8072.5 59 6130.0 60 4585.5 61 3408.0 62 2519.5 63 1906.5 64 1354.0 65 878.0 66 547.0 67 354.5 68 239.5 69 164.5 70 121.5 71 81.5 72 54.0 73 33.0 74 23.5 75 20.5 76 15.0 77 14.0 78 7.5 79 2.5 80 3.5 81 2.0 82 0.0 83 0.0 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1728253262477646 2 0.006778974241053391 3 0.002002878753038502 4 0.0 5 0.0014636421656819823 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.2147216980714204E-4 12-13 0.0 14-15 1.9258449548447134E-5 16-17 0.0 18-19 3.851689909689427E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 3.851689909689427E-5 38-39 2.5035984412981273E-4 40-41 1.1555069729068281E-4 42-43 0.001396237592262417 44-45 7.895964314863324E-4 46-47 0.005816051763631034 48-49 0.004477589520013959 50-51 0.008194470282864255 52-53 0.004679803240272653 54-55 0.02038506884703129 56-57 0.01198838484390834 58-59 0.020346551947934395 60-61 0.010004764540418286 62-63 0.006547872846472025 64-65 0.01328833018842852 66-67 0.030852036176612307 68-69 0.03197865547519647 70-71 0.036051817554693034 72-73 0.03153571113558218 74-75 0.03425115252191323 76-77 0.03385635430617006 78-79 0.017977762653475397 80-81 0.0027732167349763873 82-83 0.0 84-85 0.006278254552793765 86-87 0.02610482836292009 88-89 0.02746254905608561 90-91 0.025604108674660463 92-93 0.03484816445791509 94-95 0.031709037181518206 96-97 0.03295120717739305 98-99 0.03899836033560545 100-101 0.027269964560601143 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5192526.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.089830995801364 #Duplication Level Percentage of deduplicated Percentage of total 1 92.99440520401426 47.510684454281595 2 3.5026457939421447 3.578991633013174 3 1.0188493248736505 1.561585194539434 4 0.5099182030456931 1.0420653926114873 5 0.3242617805459017 0.8283239783243875 6 0.2537834913721245 0.7779453410235753 7 0.16903441653224616 0.6045157841174444 8 0.11291813572677825 0.46151747765136497 9 0.09600348267780384 0.44143215345156084 >10 0.8021537464666172 8.238659683735978 >50 0.09326823832366164 3.3742704709301194 >100 0.09725988367287304 10.244258997048403 >500 0.0170843220917682 6.1126370024607235 >1k 0.008104968822407865 6.178923179189989 >5k 2.703823660447756E-4 0.8877313596355296 >10k+ 3.862552608201141E-5 8.1564578979854 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 413326 7.96001791806146 TruSeq Adapter, Index 7 (97% over 35bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 8257 0.159017017921528 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 7360 0.1417421886765709 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 6476 0.12471771927574364 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 6348 0.1222526377335424 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 6009 0.11572402333661883 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 5459 0.1051318760849729 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0011169900738099336 0.0 0.0 2 0.0012903161197459578 0.0 0.0 3 0.0013866083674881936 0.0 0.0 4 0.0014829006152304292 0.0 0.0 5 0.0016754851107149006 0.0 0.0 6 0.001868069606199372 0.0 0.0 7 0.0022532385971683147 0.0 1.9258449548447134E-5 8 0.00275395828542794 0.0 3.851689909689427E-5 9 0.0030235765791062 0.0 5.77753486453414E-5 10-11 0.0058834563370506 0.0 5.77753486453414E-5 12-13 0.00826187485628382 0.0 9.629224774223567E-5 14-15 0.010736585623259277 0.0 9.629224774223567E-5 16-17 0.012315778486231941 0.0 9.629224774223567E-5 18-19 0.013894971349204605 0.0 1.155506972906828E-4 20-21 0.015628231808564846 0.0 1.3480914683912994E-4 22-23 0.017303716919279746 0.0 1.3480914683912994E-4 24-25 0.019133269626382225 0.0 1.3480914683912994E-4 26-27 0.021116889929872283 0.0 1.4443837161335352E-4 28-29 0.023581971472073512 0.0 1.733260459360242E-4 30-31 0.04025978878102873 0.0 1.8295527071024778E-4 32-33 0.04313892698852158 0.0 2.1184294503291847E-4 34-35 0.04525735643885076 0.0 2.311013945813656E-4 36-37 0.04742393201305106 0.0 2.311013945813656E-4 38-39 0.049686799834993606 0.0 2.4073061935558915E-4 40-41 0.05246964579474421 0.0 2.696182936782599E-4 42-43 0.05569543609410911 0.0 2.696182936782599E-4 44-45 0.05904640631553891 0.0 2.696182936782599E-4 46-47 0.06286920855090566 0.0 2.696182936782599E-4 48-49 0.06653794318988485 0.0 2.696182936782599E-4 50-51 0.07123700487970594 0.0 2.696182936782599E-4 52-53 0.0765715954046258 0.0 2.696182936782599E-4 54-55 0.08220469189754659 0.0 2.88876743226707E-4 56-57 0.08929180133137513 0.0 2.88876743226707E-4 58-59 0.0972840578939807 0.0 2.88876743226707E-4 60-61 0.10593110174123346 0.0 2.88876743226707E-4 62-63 0.1161573384514589 0.0 2.88876743226707E-4 64-65 0.12722131771704176 0.0 2.88876743226707E-4 66-67 0.13995115286856533 0.0 2.88876743226707E-4 68-69 0.15389427034164105 0.0 2.88876743226707E-4 70-71 0.16941658067768944 0.0 2.88876743226707E-4 72-73 0.18760618627619774 0.0 3.0813519277515415E-4 74-75 0.20736535551290453 0.0 3.1776441754937767E-4 76-77 0.23026365202600815 0.0 3.56281316646272E-4 78-79 0.2563203342650571 0.0 3.8516899096894267E-4 80-81 0.28511171633998555 0.0 3.8516899096894267E-4 82-83 0.3171000010399563 0.0 3.8516899096894267E-4 84-85 0.35345995378742445 0.0 4.0442744051738983E-4 86-87 0.39358493342161405 0.0 4.429443396142841E-4 88-89 0.4392756049753049 0.0 4.429443396142841E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 50250 0.0 88.6033 3 GATCGGA 50385 0.0 88.514206 1 CGGAAGA 50330 0.0 88.32092 4 ATCGGAA 50465 0.0 88.232605 2 GAAGAGC 51685 0.0 86.05893 6 GGAAGAG 53950 0.0 82.42988 5 AGAGCAC 69935 0.0 63.59449 8 GAGCACA 70000 0.0 63.528652 9 AAGAGCA 71625 0.0 62.054195 7 TCGGACG 160 0.0 53.427788 3 GCCGTCT 47260 0.0 46.443054 50-51 CGAGGCG 205 0.0 46.410805 1 ATGCCGT 47205 0.0 46.400223 48-49 TCGTATG 47475 0.0 46.356556 44-45 TCTCGTA 47065 0.0 46.334965 42-43 TGCCGTC 47265 0.0 46.30246 50-51 TATGCCG 47180 0.0 46.293922 48-49 CGTATGC 47340 0.0 46.22465 46-47 CGTATCT 46815 0.0 46.191982 38-39 CGTCTTC 47480 0.0 46.182613 52-53 >>END_MODULE