FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n01_52370180-2.341000000358c0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n01_52370180-2.341000000358c0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5192526
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT4133267.96001791806146TruSeq Adapter, Index 7 (97% over 35bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC82570.159017017921528No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC73600.1417421886765709No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG64760.12471771927574364No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA63480.1222526377335424No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG60090.11572402333661883No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA54590.1051318760849729No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG502500.088.60333
GATCGGA503850.088.5142061
CGGAAGA503300.088.320924
ATCGGAA504650.088.2326052
GAAGAGC516850.086.058936
GGAAGAG539500.082.429885
AGAGCAC699350.063.594498
GAGCACA700000.063.5286529
AAGAGCA716250.062.0541957
TCGGACG1600.053.4277883
GCCGTCT472600.046.44305450-51
CGAGGCG2050.046.4108051
ATGCCGT472050.046.40022348-49
TCGTATG474750.046.35655644-45
TCTCGTA470650.046.33496542-43
TGCCGTC472650.046.3024650-51
TATGCCG471800.046.29392248-49
CGTATGC473400.046.2246546-47
CGTATCT468150.046.19198238-39
CGTCTTC474800.046.18261352-53
Produced by FastQC (version 0.11.2)