##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n01_52260024-1.34100000035820.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6500620 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.680911666887155 33.0 33.0 33.0 33.0 33.0 2 32.7063896982134 33.0 33.0 33.0 33.0 33.0 3 32.73606102187176 33.0 33.0 33.0 33.0 33.0 4 36.67913291347595 37.0 37.0 37.0 37.0 37.0 5 36.64578224846245 37.0 37.0 37.0 37.0 37.0 6 36.521441339441466 37.0 37.0 37.0 37.0 37.0 7 36.45403130778295 37.0 37.0 37.0 37.0 37.0 8 36.386227467533864 37.0 37.0 37.0 37.0 37.0 9 36.419492909907056 37.0 37.0 37.0 37.0 37.0 10-11 36.3603034787451 37.0 37.0 37.0 37.0 37.0 12-13 36.33578035633524 37.0 37.0 37.0 37.0 37.0 14-15 38.16935907651886 40.0 37.0 40.0 37.0 40.0 16-17 38.15807238386492 40.0 37.0 40.0 37.0 40.0 18-19 38.15622617534943 40.0 37.0 40.0 37.0 40.0 20-21 38.09067242816839 40.0 37.0 40.0 37.0 40.0 22-23 37.96353886244697 40.0 37.0 40.0 33.0 40.0 24-25 37.97680805830828 40.0 37.0 40.0 33.0 40.0 26-27 37.89081079958527 40.0 37.0 40.0 33.0 40.0 28-29 37.75543355864517 40.0 37.0 40.0 33.0 40.0 30-31 37.69888825988906 40.0 37.0 40.0 33.0 40.0 32-33 37.57059303574121 40.0 37.0 40.0 33.0 40.0 34-35 37.46034085979491 40.0 37.0 40.0 33.0 40.0 36-37 37.320539656217406 40.0 37.0 40.0 33.0 40.0 38-39 37.27493154499109 40.0 37.0 40.0 33.0 40.0 40-41 37.218706677209255 40.0 37.0 40.0 33.0 40.0 42-43 37.02770143463239 38.5 37.0 40.0 33.0 40.0 44-45 36.92725740006338 37.0 37.0 40.0 33.0 40.0 46-47 36.86908841002858 37.0 37.0 40.0 33.0 40.0 48-49 36.61662341438201 37.0 37.0 40.0 33.0 40.0 50-51 36.470632878094705 37.0 37.0 40.0 33.0 40.0 52-53 36.34693621531484 37.0 37.0 40.0 33.0 40.0 54-55 36.13934324725949 37.0 37.0 40.0 33.0 40.0 56-57 35.954118914811204 37.0 37.0 40.0 33.0 40.0 58-59 35.73407651885512 37.0 35.0 40.0 27.0 40.0 60-61 35.413492328424056 37.0 33.0 40.0 27.0 40.0 62-63 35.17142626395636 37.0 33.0 37.0 27.0 40.0 64-65 34.867510945109856 37.0 33.0 37.0 27.0 40.0 66-67 34.429346739234106 37.0 33.0 37.0 27.0 40.0 68-69 34.15704717396187 37.0 33.0 37.0 27.0 40.0 70-71 33.82451096972289 37.0 33.0 37.0 27.0 37.0 72-73 33.30382371219976 37.0 33.0 37.0 27.0 37.0 74-75 32.73725352351006 37.0 33.0 37.0 24.5 37.0 76-77 30.07127443228492 33.0 30.0 33.0 22.0 37.0 78-79 32.47951441554805 33.0 33.0 37.0 24.5 37.0 80-81 32.904089302251165 37.0 33.0 37.0 27.0 37.0 82-83 32.85016936846024 37.0 33.0 37.0 27.0 37.0 84-85 32.72517429106762 37.0 33.0 37.0 27.0 37.0 86-87 32.56523946946599 35.0 33.0 37.0 24.5 37.0 88-89 32.44080603080937 33.0 33.0 37.0 22.0 37.0 90-91 32.30296502487455 33.0 33.0 37.0 22.0 37.0 92-93 32.14059358953453 33.0 33.0 37.0 22.0 37.0 94-95 32.04685283865231 33.0 33.0 37.0 22.0 37.0 96-97 31.910422236648195 33.0 33.0 37.0 22.0 37.0 98-99 31.61035601219576 33.0 33.0 37.0 18.5 37.0 100-101 29.962454196676624 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.09979811814955042 2306 2 0.13363390288940025 2306 3 0.11791335656209156 2306 4 0.17009599318523527 2306 5 0.19889772915970383 2306 6 0.25356928682477786 2306 7 0.27945125275964244 2306 8 0.2938407029127177 2306 9 0.2759980511162681 2306 10-11 0.2806858903877085 2306 12-13 0.2833633963681521 2306 14-15 0.5793027644472204 2306 16-17 0.5823622737105225 2306 18-19 0.5757813290402538 2306 20-21 0.5970820917007913 2306 22-23 0.6322079206375051 2306 24-25 0.5311487848436514 2306 26-27 0.649834279591559 2306 28-29 0.5794254233082583 2306 30-31 0.6147676789242595 2306 32-33 0.6893762975812621 2306 34-35 0.6951223174157519 2306 36-37 0.6370355443109617 2306 38-39 0.6964322329120307 2306 40-41 0.6393913183331179 2306 42-43 0.7702359026617174 2306 44-45 0.7976536688809119 2306 46-47 0.7507142496487873 2306 48-49 0.7507723234140897 2306 50-51 0.7361817106419934 2306 52-53 0.7853889976788864 2306 54-55 0.7103484192702467 2306 56-57 0.7307903582805508 2306 58-59 0.8261924408124983 2306 60-61 0.8207096521147292 2306 62-63 0.782416828846614 2306 64-65 0.7497801636103958 2306 66-67 0.7415672529306718 2306 68-69 0.8776245595138406 2306 70-71 0.8017902157378813 2306 72-73 0.7453040090408862 2306 74-75 0.6965465156507804 2306 76-77 0.24030543456724374 2306 78-79 0.5782717358140914 2306 80-81 0.8037690048797046 2306 82-83 0.859999752736357 2306 84-85 0.7950833907310226 2306 86-87 0.7801298922731092 2306 88-89 0.7716346472556808 2306 90-91 0.8747037487645102 2306 92-93 0.8593194807395008 2306 94-95 0.7553091549701989 2306 96-97 0.8715478341785676 2306 98-99 0.8929207802494012 2306 100-101 0.6072310621230947 2307 1 0.09129326892335143 2307 2 0.03638111513041764 2307 3 0.12781244933448477 2307 4 0.13428352936330157 2307 5 0.15215998965635436 2307 6 0.1967213020568792 2307 7 0.1995262118943586 2307 8 0.23398215687965518 2307 9 0.22031832211957436 2307 10-11 0.24337469894157238 2307 12-13 0.2235025067244507 2307 14-15 0.4852667529357717 2307 16-17 0.45268242457533603 2307 18-19 0.4154909670593554 2307 20-21 0.44238060398586043 2307 22-23 0.4536434253829782 2307 24-25 0.4579785304049011 2307 26-27 0.4338752923225968 2307 28-29 0.5309931395484284 2307 30-31 0.5476484223630607 2307 32-33 0.6203588756803882 2307 34-35 0.5793703870087015 2307 36-37 0.5329418936299959 2307 38-39 0.6238698610142421 2307 40-41 0.6690442500762614 2307 42-43 0.6009498756778768 2307 44-45 0.585027978539685 2307 46-47 0.5406850978629123 2307 48-49 0.7161310278115209 2307 50-51 0.587030916817838 2307 52-53 0.6611156762501835 2307 54-55 0.7383258969445095 2307 56-57 0.6510176521065247 2307 58-59 0.7308598665359725 2307 60-61 0.6439496005279892 2307 62-63 0.5739421735166559 2307 64-65 0.550565386940562 2307 66-67 0.5751897377586417 2307 68-69 0.6065195493748803 2307 70-71 0.4735939176415229 2307 72-73 0.4118086252712274 2307 74-75 0.4417572221742958 2307 76-77 0.129835820503029 2307 78-79 0.4570908882552729 2307 80-81 0.5907350712179991 2307 82-83 0.5948272857471366 2307 84-85 0.532498679445645 2307 86-87 0.6880884981933946 2307 88-89 0.6356806794091057 2307 90-91 0.6457921033405114 2307 92-93 0.6361793519364838 2307 94-95 0.6637231267121706 2307 96-97 0.5552792400172955 2307 98-99 0.6044902985668763 2307 100-101 0.4437792149028432 2201 1 0.1029115010491779 2201 2 0.10263458768689304 2201 3 0.10848842341136589 2201 4 0.1400089260750761 2201 5 0.16159664652747807 2201 6 0.19445332778218472 2201 7 0.18452201516752353 2201 8 0.1945858278067334 2201 9 0.20213611933514386 2201 10-11 0.21254853957573516 2201 12-13 0.17843733819297825 2201 14-15 0.38502204638813 2201 16-17 0.37784587118003543 2201 18-19 0.35350502955548535 2201 20-21 0.3069280937877963 2201 22-23 0.4546778384618051 2201 24-25 0.38911037096509205 2201 26-27 0.4282770174771571 2201 28-29 0.3618375409629877 2201 30-31 0.46573740109213446 2201 32-33 0.4515371597739204 2201 34-35 0.48650609749793006 2201 36-37 0.5041884987230105 2201 38-39 0.5118858297293514 2201 40-41 0.5100522294398857 2201 42-43 0.55342860793791 2201 44-45 0.515971186653033 2201 46-47 0.5853804923931989 2201 48-49 0.5868693037832244 2201 50-51 0.5220153700693686 2201 52-53 0.480143367339096 2201 54-55 0.5842193186376292 2201 56-57 0.5254232579088054 2201 58-59 0.4596390918266522 2201 60-61 0.6314173577170195 2201 62-63 0.5997222322249485 2201 64-65 0.5576268015807813 2201 66-67 0.4882688116602125 2201 68-69 0.6286802889862173 2201 70-71 0.6424439527756363 2201 72-73 0.47390327206834826 2201 74-75 0.44735115270810155 2201 76-77 0.27125991183848797 2201 78-79 0.5228108834042615 2201 80-81 0.6468329845314358 2201 82-83 0.49967031622686875 2201 84-85 0.3724496106488431 2201 86-87 0.5665126125496371 2201 88-89 0.727510454338443 2201 90-91 0.7457816526319903 2201 92-93 0.7262209351189384 2201 94-95 0.7264905936971289 2201 96-97 0.6509586637618234 2201 98-99 0.7722366349418941 2201 100-101 0.6264865078936559 1101 1 0.10599428816126277 1101 2 0.09669668772693996 1101 3 0.09293693922362678 1101 4 0.14682839600826014 1101 5 0.18146193962039803 1101 6 0.08827976556510464 1101 7 0.10608087619170448 1101 8 0.15143218723414265 1101 9 0.1867528111227017 1101 10-11 0.1739684000094357 1101 12-13 0.14190559005604086 1101 14-15 0.2943664868493556 1101 16-17 0.3479709606607386 1101 18-19 0.31427959335636757 1101 20-21 0.3613549951816779 1101 22-23 0.35445803875587956 1101 24-25 0.3226202280861301 1101 26-27 0.42210480183882026 1101 28-29 0.372353003620546 1101 30-31 0.3330730124610213 1101 32-33 0.37843751078821697 1101 34-35 0.4201578759680089 1101 36-37 0.38853103366554365 1101 38-39 0.44274858016693486 1101 40-41 0.42597161503992 1101 42-43 0.42365594226417613 1101 44-45 0.38631508420152016 1101 46-47 0.3517450375311597 1101 48-49 0.5029128088149832 1101 50-51 0.42120532611419037 1101 52-53 0.42374705794550493 1101 54-55 0.4094580610106533 1101 56-57 0.405853908426721 1101 58-59 0.5712590710593304 1101 60-61 0.37353192251394063 1101 62-63 0.5356030924999544 1101 64-65 0.4684940103649211 1101 66-67 0.40431968994241174 1101 68-69 0.4636428301295936 1101 70-71 0.4339609238575264 1101 72-73 0.42839269789411816 1101 74-75 0.3064245338213212 1101 76-77 0.04230253738601064 1101 78-79 0.4821840173361167 1101 80-81 0.3965923293213507 1101 82-83 0.4005925470233649 1101 84-85 0.44095349250967786 1101 86-87 0.4111763021307411 1101 88-89 0.48665752038670007 1101 90-91 0.49377022808231175 1101 92-93 0.5248167755006179 1101 94-95 0.5647870881611325 1101 96-97 0.6169469389397229 1101 98-99 0.567836538881064 1101 100-101 0.5793726999984088 2304 1 0.08879519417293835 2304 2 0.0894824409010937 2304 3 0.11003791796559881 2304 4 0.12361295224955882 2304 5 0.13957550693748288 2304 6 0.13661607045050772 2304 7 0.12149803638537549 2304 8 0.1328851473571575 2304 9 0.1324857704145117 2304 10-11 0.1853665921420955 2304 12-13 0.17667363028628102 2304 14-15 0.3140480860846466 2304 16-17 0.29375174739472953 2304 18-19 0.2807129079876276 2304 20-21 0.34747770573587644 2304 22-23 0.40363686800592546 2304 24-25 0.31401077314774994 2304 26-27 0.37074597549798227 2304 28-29 0.28444413675854463 2304 30-31 0.358494287111391 2304 32-33 0.42280495255202766 2304 34-35 0.47309015367306273 2304 36-37 0.43310893612382984 2304 38-39 0.49698194051437383 2304 40-41 0.39538810195885077 2304 42-43 0.4534958441821786 2304 44-45 0.4213396337931954 2304 46-47 0.40591015608153924 2304 48-49 0.5028109199053148 2304 50-51 0.4361893129811705 2304 52-53 0.52396238949175 2304 54-55 0.43838993973808016 2304 56-57 0.5142719372279174 2304 58-59 0.5093555987072378 2304 60-61 0.6012731024071272 2304 62-63 0.4937212148115293 2304 64-65 0.5256322103940221 2304 66-67 0.4743356739833118 2304 68-69 0.6302847934319686 2304 70-71 0.5737448941004573 2304 72-73 0.549502254654918 2304 74-75 0.4807599659431716 2304 76-77 0.2946106977251368 2304 78-79 0.5449644258725712 2304 80-81 0.4638251452305795 2304 82-83 0.5036023843153004 2304 84-85 0.45418836149124786 2304 86-87 0.619555330869602 2304 88-89 0.6562162262030498 2304 90-91 0.5518271405773731 2304 92-93 0.6292516444821885 2304 94-95 0.649347751461427 2304 96-97 0.5258223955820789 2304 98-99 0.5692131779102176 2304 100-101 0.6522336937020405 2202 1 0.05233176024053421 2202 2 0.07294540009630168 2202 3 0.07947325197712729 2202 4 0.0971582444390009 2202 5 0.12058728494132964 2202 6 0.1137007319176675 2202 7 0.1066478046684054 2202 8 0.1177216795149505 2202 9 0.07886298302896222 2202 10-11 0.13752406925298288 2202 12-13 0.13718639167432656 2202 14-15 0.16144535069412314 2202 16-17 0.19509986540084157 2202 18-19 0.17644190086656408 2202 20-21 0.1566938468006498 2202 22-23 0.2459778649540496 2202 24-25 0.2392768746510754 2202 26-27 0.23409187806382903 2202 28-29 0.2363755937496208 2202 30-31 0.22956674105058283 2202 32-33 0.23325803269219136 2202 34-35 0.2855813936414151 2202 36-37 0.2781452648228253 2202 38-39 0.2372658785724724 2202 40-41 0.16199097298858334 2202 42-43 0.22051309296707444 2202 44-45 0.2505857511211005 2202 46-47 0.227348835859992 2202 48-49 0.2706920999663538 2202 50-51 0.22350234564761706 2202 52-53 0.32891055271092284 2202 54-55 0.27681051168337234 2202 56-57 0.29529248656668017 2202 58-59 0.3442353545566803 2202 60-61 0.23092799106994732 2202 62-63 0.31217859019301386 2202 64-65 0.24433206345155156 2202 66-67 0.19021576893074865 2202 68-69 0.27184048462672195 2202 70-71 0.27844298384839306 2202 72-73 0.15431486910049586 2202 74-75 0.1938134303944068 2202 76-77 0.06151378351693282 2202 78-79 0.31644714317787503 2202 80-81 0.3851061218328198 2202 82-83 0.47637919001215323 2202 84-85 0.37636283871516696 2202 86-87 0.302494632327722 2202 88-89 0.36483718245940366 2202 90-91 0.4200967008803076 2202 92-93 0.31593992299468 2202 94-95 0.4391828141945595 2202 96-97 0.3680242267112739 2202 98-99 0.29177766304642105 2202 100-101 0.26168272727274555 1102 1 0.11400560835703288 1102 2 0.06716558104481152 1102 3 0.03740994699820277 1102 4 0.10100827958859782 1102 5 0.0952997982746453 1102 6 0.09521542768879954 1102 7 0.1019905796273477 1102 8 0.09915702833791329 1102 9 0.088501543353523 1102 10-11 0.049916001740193394 1102 12-13 0.03471507036139343 1102 14-15 0.15863980240284548 1102 16-17 0.10590822014957979 1102 18-19 0.16387629223635258 1102 20-21 0.1851236379209098 1102 22-23 0.18022536329354466 1102 24-25 0.1585676936691769 1102 26-27 0.2489665655387867 1102 28-29 0.186998731782559 1102 30-31 0.14959066525749876 1102 32-33 0.11880609363461048 1102 34-35 0.10776642565515004 1102 36-37 0.24367339645676367 1102 38-39 0.22207124105963771 1102 40-41 0.2858805152040773 1102 42-43 0.1829892324030311 1102 44-45 0.18344332786750783 1102 46-47 0.16160625256801353 1102 48-49 0.1918511538994636 1102 50-51 0.1694496892495465 1102 52-53 0.22647463446340765 1102 54-55 0.22637315571324024 1102 56-57 0.24847109224904784 1102 58-59 0.1588916836384442 1102 60-61 0.02279006337199263 1102 62-63 0.13754909911441615 1102 64-65 0.16104890053232168 1102 66-67 0.2007951691625962 1102 68-69 0.31961172803637794 1102 70-71 0.2920948742398366 1102 72-73 0.06706000571114856 1102 74-75 0.04088331841492021 1102 76-77 -0.08781733824062954 1102 78-79 0.11234695575377174 1102 80-81 0.030689450289621334 1102 82-83 0.145569365140787 1102 84-85 0.1373394971572992 1102 86-87 0.03770309384219672 1102 88-89 0.06678938187415184 1102 90-91 0.13304713118871803 1102 92-93 0.19764086919601453 1102 94-95 0.21504793562349533 1102 96-97 0.20233856934440198 1102 98-99 0.12882867641164353 1102 100-101 0.10133441951722943 2305 1 0.0214655038268603 2305 2 -0.011875197343400146 2305 3 0.036006038390382855 2305 4 0.07286391282968907 2305 5 0.06016564337766539 2305 6 0.03664933848501306 2305 7 0.010013003564118605 2305 8 0.01575791087229561 2305 9 -0.021791330317519453 2305 10-11 0.0500137104017071 2305 12-13 0.048995739094749524 2305 14-15 0.038903693191912225 2305 16-17 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-2.1436546846449716 1213 78-79 -1.7860224003582097 1213 80-81 -1.213475672049043 1213 82-83 -0.8116906104307517 1213 84-85 -0.5280725452999491 1213 86-87 -0.38067832353786457 1213 88-89 -0.406502162588545 1213 90-91 -1.6353073505687625 1213 92-93 -0.7870288770200489 1213 94-95 -0.3410524528584773 1213 96-97 -0.43248998036655095 1213 98-99 -2.182869231118776 1213 100-101 -0.8364857166890864 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 9.0 8 107.0 9 313.0 10 210.0 11 107.0 12 103.0 13 195.0 14 485.0 15 1030.0 16 2092.0 17 3736.0 18 5684.0 19 8075.0 20 11378.0 21 15335.0 22 20276.0 23 26317.0 24 34323.0 25 44545.0 26 58729.0 27 80213.0 28 117239.0 29 161951.0 30 159785.0 31 162503.0 32 215033.0 33 306062.0 34 471727.0 35 812443.0 36 1486163.0 37 1923372.0 38 370690.0 39 390.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.423704716428986 25.803749387065487 21.897287739737912 15.875258156767613 2 29.568970583393817 25.00042306351522 31.120244496866718 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30.522534773606207 19.376190271081835 22-23 21.95418283179143 29.862182376450246 28.680448941793244 19.503185849965078 24-25 21.82958702400694 26.206069574902084 30.728792022914735 21.235551378176236 26-27 21.68656989640988 26.35927803809483 29.158695632108937 22.795456433386356 28-29 22.833475883838773 28.420819860259485 28.655774372290644 20.089929883611102 30-31 20.863417643240183 27.503907319609517 30.16550729007387 21.46716774707643 32-33 20.691072543849664 28.96475259282961 28.941054853229385 21.403120010091346 34-35 22.527328162544496 29.123122102199485 29.09684768529771 19.252702049958312 36-37 22.224814283070582 29.45618717232928 29.423836406963705 18.895162137636433 38-39 21.06081422317721 27.570096980263827 31.971789992956033 19.39729880360293 40-41 21.035807249503165 27.256099465111816 28.680919378193835 23.027173907191187 42-43 21.30703252171033 28.926790558530225 30.184794804181102 19.581382115578343 44-45 20.88953635273174 27.51439391346384 30.45353278227973 21.14253695152469 46-47 22.488955983852595 27.431750783951887 29.007800404892777 21.071492827302748 48-49 21.071694930794475 29.293047079940948 30.02671549277054 19.60854249649403 50-51 22.451629637509875 27.821993896844816 29.43888925683305 20.28748720881226 52-53 20.798576262592388 27.497932420932024 28.32823309984869 23.375258216626904 54-55 22.542098445844985 27.53938316157561 29.790426520807294 20.128091871772114 56-57 21.219903118897047 27.275610522335175 29.47331246540345 22.031173893364326 58-59 21.18134893602126 27.405350493110227 29.60508484942434 21.808215721444167 60-61 22.37325068955499 27.65556073587711 29.485638171727174 20.485550402840726 62-63 20.818790586522777 27.720042672313234 29.524193246288768 21.93697349487522 64-65 22.686402176070303 27.65890128758006 29.404026899330592 20.25066963701904 66-67 21.688694946739428 30.445628730794372 27.71430101424673 20.151375308219475 68-69 21.74655517199115 30.364051407495506 27.720798797084857 20.168594623428486 70-71 21.428340048325165 30.91618762253741 27.775051838793107 19.880420490344317 72-73 21.384310691043567 31.238310435141685 27.420909891992633 19.956468981822116 74-75 21.932649135935723 30.484993499186032 27.246397084089864 20.33596028078838 76-77 22.05161613576688 29.593949802975576 27.74508870061444 20.609345360643097 78-79 21.78924275100481 29.259909282071035 28.475639692794214 20.475208274129944 80-81 21.3715672954957 29.529048560115324 28.287291908267843 20.812092236121135 82-83 20.506513224892394 29.59421562866311 28.56272940119558 21.336541745248915 84-85 20.63663743217708 29.564125652153113 28.51005792284205 21.289178992827757 86-87 20.483748217780253 29.646742734713687 29.037672117112418 20.831836930393642 88-89 20.619568302394054 29.938566648481345 28.847993673939694 20.593871375184907 90-91 20.9967790864115 29.39067300668225 28.390954976067224 21.221592930839027 92-93 20.64719991788701 29.907316658056804 28.0309056296178 21.414577794438383 94-95 20.386769704719654 30.08935812032493 28.02316509632119 21.500707078634225 96-97 20.708228475511667 30.54472567593478 27.660644439732234 21.08640140882132 98-99 20.93262753646551 29.940424824669705 27.62783078264891 21.499116856215885 100-101 21.032935404415316 29.902148185277184 27.2420113843087 21.8229050259988 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 26.0 1 16.5 2 15.5 3 60.5 4 83.5 5 56.0 6 41.5 7 48.5 8 50.0 9 46.5 10 54.0 11 54.5 12 48.0 13 47.0 14 69.0 15 89.0 16 87.0 17 106.0 18 151.5 19 200.0 20 293.0 21 439.0 22 663.0 23 1015.0 24 1484.5 25 2180.0 26 3493.5 27 5541.5 28 7765.0 29 10020.5 30 12839.5 31 17146.0 32 28465.5 33 51020.0 34 87272.0 35 140159.5 36 214548.0 37 308427.5 38 395834.0 39 431345.5 40 440789.0 41 430836.0 42 408909.5 43 418051.5 44 416306.0 45 402159.0 46 400007.0 47 362694.0 48 291070.5 49 238675.0 50 199014.5 51 168832.5 52 147135.0 53 119732.5 54 87425.5 55 59509.5 56 42535.0 57 31976.0 58 25513.5 59 20339.0 60 15725.0 61 12321.0 62 9462.0 63 7207.0 64 5808.0 65 4557.0 66 3312.0 67 2459.5 68 1954.5 69 1437.5 70 969.5 71 723.5 72 548.5 73 384.0 74 276.5 75 201.0 76 136.5 77 97.0 78 68.5 79 47.5 80 35.5 81 22.0 82 14.0 83 13.0 84 11.0 85 6.0 86 6.0 87 5.5 88 4.5 89 4.5 90 1.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17453719799034553 2 0.006368623300546717 3 0.0020921081373776657 4 0.0 5 0.0010306709206198794 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.2305564699982465E-4 12-13 0.0 14-15 7.69157403447671E-6 16-17 0.0 18-19 6.153259227581369E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 1.538314806895342E-5 38-39 2.92279813310115E-4 40-41 1.3844833262058082E-4 42-43 0.0013152591598955177 44-45 6.537837929305205E-4 46-47 0.005853287840236777 48-49 0.0041842162747553315 50-51 0.008299208383200371 52-53 0.004061151090203704 54-55 0.020005784063673926 56-57 0.010675904759853675 58-59 0.020190381840501367 60-61 0.00914528152699281 62-63 0.0059455867286504985 64-65 0.01276801289723134 66-67 0.03082782873018266 68-69 0.031896957520974926 70-71 0.03614270638800607 72-73 0.03131239789435469 74-75 0.033889075195904395 76-77 0.033642944826801136 78-79 0.017429106762124228 80-81 0.0023305469324464436 82-83 0.0 84-85 0.00647630533702939 86-87 0.026182118013358728 88-89 0.027512760321323198 90-91 0.0259590623663589 92-93 0.03471976519162787 94-95 0.031535453541354515 96-97 0.03288917057142242 98-99 0.038880906744279776 100-101 0.027112798471530405 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6500620.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.34392590104191 #Duplication Level Percentage of deduplicated Percentage of total 1 85.27626399703372 27.58169163837747 2 6.1576744232609215 3.983267305373844 3 2.4563078169494967 2.3833991406469357 4 1.207592396630012 1.5623311598105085 5 0.7927653681466443 1.282057216212364 6 0.6073753150377905 1.1786941310222572 7 0.40111736106799084 0.9081597142803198 8 0.27049253631438325 0.6999032441069841 9 0.30166671336437406 0.8781377241481348 >10 1.9624821731162034 12.778167939099674 >50 0.2533487985882234 5.635499032484894 >100 0.24605593257785172 17.533511153200504 >500 0.04742682900034238 10.615014021927527 >1k 0.019145629197273827 9.867731601233157 >5k 2.3726878232842736E-4 0.4388818202250193 >10k+ 4.74409320468628E-5 2.6735531578502694 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 174238 2.6803289532383068 TruSeq Adapter, Index 7 (97% over 38bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 6610 0.10168260873578212 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0016152305472401094 0.0 0.0 2 0.0017229125837227833 0.0 0.0 3 0.001861360916343364 0.0 0.0 4 0.0020151923970328986 0.0 0.0 5 0.0023536216545498737 0.0 0.0 6 0.0026766677639978955 0.0 0.0 7 0.0032150779464112655 0.0 0.0 8 0.003815020721100449 0.0 0.0 9 0.004430346643858586 0.0 0.0 10-11 0.007068556537684098 0.0 0.0 12-13 0.0092145056933031 0.0 0.0 14-15 0.011875790309232043 0.0 0.0 16-17 0.013944823724506276 0.0 0.0 18-19 0.016967612320055624 0.0 0.0 20-21 0.020359596469259854 0.0 0.0 22-23 0.02388233737705019 0.0 0.0 24-25 0.028005021059529706 0.0 0.0 26-27 0.0324661339995262 0.0 0.0 28-29 0.0377502453612117 0.0 0.0 30-31 0.0662552187329824 0.0 0.0 32-33 0.07317763536401142 0.0 0.0 34-35 0.07850789616990379 0.0 0.0 36-37 0.08426888512172685 0.0 0.0 38-39 0.09134513323344542 0.0 0.0 40-41 0.09940590282157702 0.0 0.0 42-43 0.10845888546015611 0.0 0.0 44-45 0.11848100642707926 0.0 0.0 46-47 0.12914152803886397 0.0 0.0 48-49 0.14020970307447597 0.0 1.538314806895342E-5 50-51 0.15353920087622414 0.0 1.538314806895342E-5 52-53 0.16745325830459248 0.0 1.538314806895342E-5 54-55 0.18305946202054574 0.0 1.538314806895342E-5 56-57 0.20045780248653206 0.0 1.538314806895342E-5 58-59 0.2207635579375506 0.0 1.538314806895342E-5 60-61 0.2428076091203608 0.0 1.538314806895342E-5 62-63 0.2674283376047208 0.0 1.538314806895342E-5 64-65 0.2948334158895613 0.0 1.538314806895342E-5 66-67 0.32526897434398566 0.0 1.538314806895342E-5 68-69 0.3577735662136842 0.0 1.538314806895342E-5 70-71 0.3942700850072762 0.0 1.538314806895342E-5 72-73 0.4338739997107968 0.0 1.538314806895342E-5 74-75 0.4788543246644166 0.0 1.538314806895342E-5 76-77 0.5295110312554803 0.0 1.538314806895342E-5 78-79 0.5856056806889189 0.0 1.538314806895342E-5 80-81 0.64732287074156 0.0 1.538314806895342E-5 82-83 0.7165162707557124 0.0 1.538314806895342E-5 84-85 0.7958471653473054 0.0 1.538314806895342E-5 86-87 0.8823312237909615 0.0 1.538314806895342E-5 88-89 0.976806827656439 0.0 1.538314806895342E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 27615 0.0 67.43291 4 TCGGAAG 27885 0.0 66.93635 3 GAAGAGC 27875 0.0 66.80444 6 GATCGGA 28540 0.0 65.40843 1 ATCGGAA 29005 0.0 64.3725 2 GGAAGAG 30835 0.0 60.48398 5 TTGTGTT 60590 0.0 45.37021 1 ATGCCGT 20695 0.0 44.62957 48-49 TATGCCG 20700 0.0 44.538483 48-49 GCCGTCT 20790 0.0 44.519253 50-51 TCGTATG 20845 0.0 44.48678 44-45 CGTATGC 20775 0.0 44.46675 46-47 TGCCGTC 20785 0.0 44.438545 50-51 CGTCTTC 21065 0.0 43.973236 52-53 CTCGTAT 20750 0.0 43.96945 44-45 TCTCGTA 20750 0.0 43.96861 42-43 CATCTCG 20685 0.0 43.842392 40-41 ATCTCGT 20730 0.0 43.747555 42-43 GGTACGC 55 4.913803E-5 43.25902 1 CTTGAAA 20585 0.0 42.87922 62-63 >>END_MODULE