FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n01_52260024-1.34100000035820.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n01_52260024-1.34100000035820.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6500620
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT1742382.6803289532383068TruSeq Adapter, Index 7 (97% over 38bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC66100.10168260873578212No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA276150.067.432914
TCGGAAG278850.066.936353
GAAGAGC278750.066.804446
GATCGGA285400.065.408431
ATCGGAA290050.064.37252
GGAAGAG308350.060.483985
TTGTGTT605900.045.370211
ATGCCGT206950.044.6295748-49
TATGCCG207000.044.53848348-49
GCCGTCT207900.044.51925350-51
TCGTATG208450.044.4867844-45
CGTATGC207750.044.4667546-47
TGCCGTC207850.044.43854550-51
CGTCTTC210650.043.97323652-53
CTCGTAT207500.043.9694544-45
TCTCGTA207500.043.9686142-43
CATCTCG206850.043.84239240-41
ATCTCGT207300.043.74755542-43
GGTACGC554.913803E-543.259021
CTTGAAA205850.042.8792262-63
Produced by FastQC (version 0.11.2)