##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n01_52150223-1.3410000003573e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3118302 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.696967131470906 33.0 33.0 33.0 33.0 33.0 2 32.71928665023465 33.0 33.0 33.0 33.0 33.0 3 32.75164688987789 33.0 33.0 33.0 33.0 33.0 4 36.688762666348545 37.0 37.0 37.0 37.0 37.0 5 36.65437472060115 37.0 37.0 37.0 37.0 37.0 6 36.53356858957215 37.0 37.0 37.0 37.0 37.0 7 36.46302891766096 37.0 37.0 37.0 37.0 37.0 8 36.4023600023346 37.0 37.0 37.0 37.0 37.0 9 36.434502174580906 37.0 37.0 37.0 37.0 37.0 10-11 36.36553948270565 37.0 37.0 37.0 37.0 37.0 12-13 36.339208165212995 37.0 37.0 37.0 37.0 37.0 14-15 38.158380105583106 40.0 37.0 40.0 37.0 40.0 16-17 38.16234941323836 40.0 37.0 40.0 37.0 40.0 18-19 38.14461187530907 40.0 37.0 40.0 37.0 40.0 20-21 38.08917625682182 40.0 37.0 40.0 37.0 40.0 22-23 37.9380608420865 40.0 37.0 40.0 33.0 40.0 24-25 37.95384908196833 40.0 37.0 40.0 33.0 40.0 26-27 37.879541494056696 40.0 37.0 40.0 33.0 40.0 28-29 37.70505406468007 40.0 37.0 40.0 33.0 40.0 30-31 37.636408372248745 40.0 37.0 40.0 33.0 40.0 32-33 37.50992174587323 40.0 37.0 40.0 33.0 40.0 34-35 37.46007346305778 40.0 37.0 40.0 33.0 40.0 36-37 37.38363731287092 40.0 37.0 40.0 33.0 40.0 38-39 37.18853545936218 40.0 37.0 40.0 33.0 40.0 40-41 37.06797128693757 40.0 37.0 40.0 33.0 40.0 42-43 36.92073650980566 37.0 37.0 40.0 33.0 40.0 44-45 36.773044272171205 37.0 37.0 40.0 33.0 40.0 46-47 36.77093334770012 37.0 37.0 40.0 33.0 40.0 48-49 36.49167816330811 37.0 37.0 40.0 33.0 40.0 50-51 36.402764709768334 37.0 37.0 40.0 33.0 40.0 52-53 36.33715688858872 37.0 37.0 40.0 33.0 40.0 54-55 36.04942305139143 37.0 37.0 40.0 30.0 40.0 56-57 35.871526715500934 37.0 37.0 40.0 27.0 40.0 58-59 35.72232917145293 37.0 37.0 40.0 27.0 40.0 60-61 35.467065248972034 37.0 33.0 40.0 27.0 40.0 62-63 35.274998380528885 37.0 33.0 40.0 27.0 40.0 64-65 35.03011302304908 37.0 33.0 40.0 27.0 40.0 66-67 34.548772697448804 37.0 33.0 38.5 27.0 40.0 68-69 34.356533780243225 37.0 33.0 37.0 27.0 40.0 70-71 34.089981182066396 37.0 33.0 37.0 27.0 40.0 72-73 33.55130612750144 37.0 33.0 37.0 24.5 40.0 74-75 32.897511530313615 37.0 33.0 37.0 22.0 40.0 76-77 30.29485021014642 33.0 30.0 35.0 18.5 37.0 78-79 32.47305841448327 35.0 33.0 37.0 22.0 37.0 80-81 32.74756453993231 37.0 33.0 37.0 22.0 37.0 82-83 32.61057428048983 37.0 33.0 37.0 22.0 37.0 84-85 32.431256978958416 37.0 33.0 37.0 22.0 37.0 86-87 32.220877740513906 37.0 33.0 37.0 22.0 37.0 88-89 32.02973156544812 35.0 33.0 37.0 22.0 37.0 90-91 31.84635243796143 33.0 33.0 37.0 15.0 37.0 92-93 31.674925167607242 33.0 33.0 37.0 15.0 37.0 94-95 31.564949129365917 33.0 33.0 37.0 15.0 37.0 96-97 31.421772009253754 33.0 33.0 37.0 15.0 37.0 98-99 31.108712850775838 33.0 33.0 37.0 15.0 37.0 100-101 29.493843925315765 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.13653488692417426 2306 2 0.17561667595204256 2306 3 0.15209997822624644 2306 4 0.14282410470863738 2306 5 0.21443164571215334 2306 6 0.20805746151527416 2306 7 0.22189135472038402 2306 8 0.26081544045426597 2306 9 0.23926916165279977 2306 10-11 0.2167080277857778 2306 12-13 0.17712506197928946 2306 14-15 0.504717226250051 2306 16-17 0.4833567265685659 2306 18-19 0.6049462797630696 2306 20-21 0.49914001395869434 2306 22-23 0.506613227245829 2306 24-25 0.503898645916216 2306 26-27 0.4533246802026625 2306 28-29 0.535425459167655 2306 30-31 0.5345734905959247 2306 32-33 0.4926673348710082 2306 34-35 0.553027579161558 2306 36-37 0.5234835432648453 2306 38-39 0.6462806358890205 2306 40-41 0.658677915460018 2306 42-43 0.7779660563603343 2306 44-45 0.7478560656409243 2306 46-47 0.7016920104468696 2306 48-49 0.7507210670639068 2306 50-51 0.48388020916615915 2306 52-53 0.667514342357542 2306 54-55 0.6963650342306451 2306 56-57 0.6957042380297835 2306 58-59 0.5608850052542422 2306 60-61 0.7030508897591403 2306 62-63 0.6446700473068248 2306 64-65 0.6569741277064622 2306 66-67 0.7241324562338534 2306 68-69 0.8010252497222652 2306 70-71 0.44481255383577434 2306 72-73 0.34067875402077163 2306 74-75 0.5256535531805895 2306 76-77 0.2023908195550881 2306 78-79 0.6591433196522445 2306 80-81 0.6259429479417022 2306 82-83 0.6726892445136201 2306 84-85 0.5654032233451431 2306 86-87 0.4891423591747426 2306 88-89 0.5892330289013685 2306 90-91 0.9340604771777912 2306 92-93 0.7785334479232695 2306 94-95 0.8300525198217841 2306 96-97 0.8387422507673818 2306 98-99 0.9796534500393648 2306 100-101 0.5063017908767904 2307 1 0.11180093533885582 2307 2 0.03954412492043957 2307 3 0.10062211074384919 2307 4 0.09656878858052664 2307 5 0.1879177995781589 2307 6 0.19698797592932493 2307 7 0.1800864203209187 2307 8 0.23985908594676886 2307 9 0.18757122744049326 2307 10-11 0.19126482888029983 2307 12-13 0.23543758255004832 2307 14-15 0.3911744100412662 2307 16-17 0.4052101272771722 2307 18-19 0.3724875664758116 2307 20-21 0.4287128521741579 2307 22-23 0.4507771027435652 2307 24-25 0.4320826759124117 2307 26-27 0.4665469153320245 2307 28-29 0.49066640319585275 2307 30-31 0.48960098280323905 2307 32-33 0.5224762124016209 2307 34-35 0.518578262271923 2307 36-37 0.445703668076888 2307 38-39 0.5983783687499553 2307 40-41 0.5046078103313718 2307 42-43 0.46210576323920094 2307 44-45 0.4920431739899058 2307 46-47 0.38063465757212356 2307 48-49 0.391859509043087 2307 50-51 0.45534639568722923 2307 52-53 0.3671548785201253 2307 54-55 0.34905649551244977 2307 56-57 0.4264275863999316 2307 58-59 0.524719684156878 2307 60-61 0.46711999458447906 2307 62-63 0.3965659447725045 2307 64-65 0.4532636477544756 2307 66-67 0.45210077565975126 2307 68-69 0.5706053804715907 2307 70-71 0.4384935409738091 2307 72-73 0.4262356648259811 2307 74-75 0.27265570867290023 2307 76-77 0.029543730437744387 2307 78-79 0.24002042432269377 2307 80-81 0.39824003157351484 2307 82-83 0.3693514582171318 2307 84-85 0.5700678635909 2307 86-87 0.4013073382329111 2307 88-89 0.49755926330793443 2307 90-91 0.5646825045022048 2307 92-93 0.5416929220864013 2307 94-95 0.5479929598261748 2307 96-97 0.5436017888208582 2307 98-99 0.3446173423163792 2307 100-101 0.07905030171556149 2201 1 0.10333663951723793 2201 2 0.09520589726977846 2201 3 0.1013893087873825 2201 4 0.1488407144042867 2201 5 0.16197954248301016 2201 6 0.1688443664030359 2201 7 0.23107014975391849 2201 8 0.15868754344847247 2201 9 0.18710543751000586 2201 10-11 0.21266132329505183 2201 12-13 0.2666359334597104 2201 14-15 0.44424747962426636 2201 16-17 0.4057428138928074 2201 18-19 0.31095554783227897 2201 20-21 0.3713598868604606 2201 22-23 0.4515119715340532 2201 24-25 0.36116920540688824 2201 26-27 0.3940251888305468 2201 28-29 0.3440486399126286 2201 30-31 0.4797888058159998 2201 32-33 0.4971231147866675 2201 34-35 0.5363482848974215 2201 36-37 0.5447087726172555 2201 38-39 0.48931725004838 2201 40-41 0.5053186808686192 2201 42-43 0.694835351916602 2201 44-45 0.5114724333565661 2201 46-47 0.563371657109073 2201 48-49 0.6896155355888851 2201 50-51 0.623611329439612 2201 52-53 0.6726423215895565 2201 54-55 0.6530406283150612 2201 56-57 0.7902723154633762 2201 58-59 0.5898054337702945 2201 60-61 0.6617715673031626 2201 62-63 0.575546285105041 2201 64-65 0.48078566521923705 2201 66-67 0.567287417411336 2201 68-69 0.6357652386804631 2201 70-71 0.5924315175505157 2201 72-73 0.49532688454901574 2201 74-75 0.43983375780639733 2201 76-77 0.2752112374991995 2201 78-79 0.41881723397252557 2201 80-81 0.487259624592987 2201 82-83 0.4728390136928837 2201 84-85 0.31278196904295186 2201 86-87 0.525411755751783 2201 88-89 0.7101510846037584 2201 90-91 0.733119280668884 2201 92-93 0.6027361472924788 2201 94-95 0.6633438590519134 2201 96-97 0.6276082337817996 2201 98-99 0.6380387620878558 2201 100-101 0.5406189021523531 1101 1 0.09824953401373193 1101 2 0.10547864631141834 1101 3 0.09905342759246594 1101 4 0.13766586554891802 1101 5 0.14850187035401774 1101 6 0.10590865346446066 1101 7 0.10244347328289649 1101 8 0.15669792278811912 1101 9 0.14724161071656283 1101 10-11 0.14425346483381674 1101 12-13 0.1856543039161025 1101 14-15 0.34253789770100695 1101 16-17 0.3275413343389175 1101 18-19 0.23685876017220409 1101 20-21 0.286962060206875 1101 22-23 0.2750458209382671 1101 24-25 0.27002364247923083 1101 26-27 0.24752922253266973 1101 28-29 0.3293337326973216 1101 30-31 0.24338525336091976 1101 32-33 0.36259708273319546 1101 34-35 0.4251515033827431 1101 36-37 0.4195976789298328 1101 38-39 0.4937358862395982 1101 40-41 0.4306093680707548 1101 42-43 0.4377614865343915 1101 44-45 0.5091864569082176 1101 46-47 0.4997837919091026 1101 48-49 0.35931148747690145 1101 50-51 0.37304955668739836 1101 52-53 0.3571153762050514 1101 54-55 0.28800626714879485 1101 56-57 0.351432330636122 1101 58-59 0.4506998200690546 1101 60-61 0.24104753525392653 1101 62-63 0.39312133824140716 1101 64-65 0.19367708901966552 1101 66-67 0.19784289644392317 1101 68-69 0.4894260855989785 1101 70-71 0.37667072975482085 1101 72-73 0.18639351109410285 1101 74-75 0.1842288508241836 1101 76-77 0.08016653513704242 1101 78-79 0.5728844178402639 1101 80-81 0.3855995235967953 1101 82-83 0.4255556967119176 1101 84-85 0.2732175707289102 1101 86-87 0.3503428321049924 1101 88-89 0.5247735991672542 1101 90-91 0.4489543428326037 1101 92-93 0.5204826354181158 1101 94-95 0.4654927298907978 1101 96-97 0.33170603860867587 1101 98-99 0.3212231935022061 1101 100-101 0.37249277770623124 2304 1 0.07473238556824668 2304 2 0.0468245011471069 2304 3 0.047653332073060994 2304 4 0.102757301643436 2304 5 0.12879484173404165 2304 6 0.07894738144825908 2304 7 0.06085300739814414 2304 8 0.12775302469647443 2304 9 0.09698910430097385 2304 10-11 0.12304680095071063 2304 12-13 0.1628789324221529 2304 14-15 0.30245859528328367 2304 16-17 0.2831803213682349 2304 18-19 0.29611353444706623 2304 20-21 0.24256200634400216 2304 22-23 0.2564672969164903 2304 24-25 0.2842309428354426 2304 26-27 0.2660719025591405 2304 28-29 0.36597094593324186 2304 30-31 0.295788108867967 2304 32-33 0.3619042279914808 2304 34-35 0.383519768465554 2304 36-37 0.3331368278383877 2304 38-39 0.4108465502157017 2304 40-41 0.3149354670525 2304 42-43 0.3737645516803667 2304 44-45 0.35941329127310695 2304 46-47 0.14252833756709293 2304 48-49 0.27129616949706303 2304 50-51 0.2848742001122986 2304 52-53 0.3120065799917313 2304 54-55 0.35095452295091434 2304 56-57 0.4689648881245603 2304 58-59 0.3757976887075998 2304 60-61 0.35704645412153013 2304 62-63 0.3391994723386418 2304 64-65 0.293989416157153 2304 66-67 0.25932019217916746 2304 68-69 0.31550685471423634 2304 70-71 0.26799144535597463 2304 72-73 0.20519794916197753 2304 74-75 0.21677355167697954 2304 76-77 -0.04164724852603996 2304 78-79 0.23020991328662177 2304 80-81 0.12968182528504713 2304 82-83 0.2637658800878597 2304 84-85 0.20319206336683493 2304 86-87 0.23808902949303246 2304 88-89 0.419755827326874 2304 90-91 0.33085041268383364 2304 92-93 0.37682658664703084 2304 94-95 0.30946963085771984 2304 96-97 0.06884390527095263 2304 98-99 0.2596987193190827 2304 100-101 0.11819048107489039 2202 1 0.13049252216817564 2202 2 0.12557614970388187 2202 3 0.11146549533128791 2202 4 0.11496280332185194 2202 5 0.16107834578826186 2202 6 0.15571188134743608 2202 7 0.1881863660380816 2202 8 0.16291242668522443 2202 9 0.14480099452650563 2202 10-11 0.13068118161748288 2202 12-13 0.16139988924851423 2202 14-15 0.13106867778164144 2202 16-17 0.21544461910481516 2202 18-19 0.17931169802359648 2202 20-21 0.2689538897506907 2202 22-23 0.27286153453363937 2202 24-25 0.2572588649556522 2202 26-27 0.22726704397022246 2202 28-29 0.19233469025623862 2202 30-31 0.21658947375282622 2202 32-33 0.21291439671801982 2202 34-35 0.2369418710889306 2202 36-37 0.36080846493057095 2202 38-39 0.25858232909543943 2202 40-41 0.2742259094107382 2202 42-43 0.3042240998321546 2202 44-45 0.3051149591755049 2202 46-47 0.2059111882582556 2202 48-49 0.35974631195117723 2202 50-51 0.3628826703943986 2202 52-53 0.473702008539874 2202 54-55 0.3577558752985581 2202 56-57 0.46604223682387413 2202 58-59 0.4957758809177406 2202 60-61 0.3182531750441626 2202 62-63 0.345898437426321 2202 64-65 0.43768610469545877 2202 66-67 0.28786368813295127 2202 68-69 0.41727874634673157 2202 70-71 0.2676243835464476 2202 72-73 0.3424816778413131 2202 74-75 0.314118152020157 2202 76-77 0.20824958063802868 2202 78-79 0.5233996629985214 2202 80-81 0.4434809289437922 2202 82-83 0.5305948282398631 2202 84-85 0.5051021429746712 2202 86-87 0.4428050424077554 2202 88-89 0.45977514160387756 2202 90-91 0.35861320578042566 2202 92-93 0.3443223753615179 2202 94-95 0.5581419049101015 2202 96-97 0.4402486410648052 2202 98-99 0.26618638008710604 2202 100-101 0.4336886386265917 1102 1 0.09421097315427573 1102 2 0.06189418730880192 1102 3 0.07815369592834287 1102 4 0.07699914783272277 1102 5 0.12025768633458256 1102 6 0.12461575353076881 1102 7 0.10487201887637099 1102 8 0.1538790112183932 1102 9 0.0910465059319705 1102 10-11 0.1276330957457148 1102 12-13 0.07484242088904125 1102 14-15 0.19201165308361112 1102 16-17 0.16235371917199615 1102 18-19 0.097542259063502 1102 20-21 0.10569848659861236 1102 22-23 0.213850170358171 1102 24-25 0.22650785595532597 1102 26-27 0.2219283179784597 1102 28-29 0.18474134418215726 1102 30-31 0.23344436219906584 1102 32-33 0.1256703512988011 1102 34-35 0.09381054086015439 1102 36-37 0.12215843737378407 1102 38-39 0.1323870056091465 1102 40-41 0.13991216726852684 1102 42-43 0.18608467241604387 1102 44-45 0.23736156113886153 1102 46-47 0.19432298572841944 1102 48-49 0.19581282426857172 1102 50-51 0.245172847817976 1102 52-53 0.07482782506727403 1102 54-55 0.07462417608403626 1102 56-57 -0.03596712487176745 1102 58-59 0.17227992248310642 1102 60-61 0.018725476806565666 1102 62-63 0.22466611872571463 1102 64-65 0.16167758756720474 1102 66-67 0.21866743866812755 1102 68-69 0.2114558583362438 1102 70-71 0.12102883351462168 1102 72-73 -0.02901698414607523 1102 74-75 -0.04079342903693828 1102 76-77 -0.03171942455881194 1102 78-79 0.18652914244162844 1102 80-81 0.07763946279354883 1102 82-83 0.17754442888599442 1102 84-85 0.08619715797716765 1102 86-87 0.12604483101991804 1102 88-89 0.0394337477865534 1102 90-91 0.19551183953747042 1102 92-93 0.151858382221139 1102 94-95 0.1806713842360672 1102 96-97 0.21978887896104737 1102 98-99 0.10351212949424138 1102 100-101 -0.009253132493299177 2305 1 0.03857128758600936 2305 2 0.011463691352325611 2305 3 0.03449147625348559 2305 4 0.09930876806615885 2305 5 0.058596085065595105 2305 6 0.006549252275107165 2305 7 0.03279882576531179 2305 8 0.07498751631018763 2305 9 -4.979250215058073E-4 2305 10-11 0.06541618611593236 2305 12-13 0.06106931551116901 2305 14-15 0.15433540112596233 2305 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26.0 12 39.0 13 109.0 14 293.0 15 788.0 16 1418.0 17 2565.0 18 3824.0 19 5512.0 20 7579.0 21 9961.0 22 12713.0 23 16629.0 24 20953.0 25 26845.0 26 35397.0 27 48779.0 28 71128.0 29 87168.0 30 75225.0 31 77877.0 32 102330.0 33 142594.0 34 209621.0 35 334279.0 36 567590.0 37 876418.0 38 379704.0 39 898.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.023558632688285 26.285552934645978 19.538072450392693 16.15281598227304 2 29.57401151795218 27.674033678961695 28.400857063412875 14.351097739673241 3 34.84932837070166 20.994356428213624 27.532247378166772 16.624067822917944 4 29.420979751159443 21.646877050394732 28.257109157483782 20.675034040962036 5 34.38039062131706 19.942256313851654 28.7493069057998 16.92804615903149 6 31.084288821288 20.675322659575627 31.035351931916793 17.20503658721958 7 31.18780669736286 24.792210632581448 25.101096686594175 18.91888598346151 8 28.66213727855737 30.177160518769508 23.072941620150967 18.087760582522154 9 31.903933615153374 24.433650108296117 24.768191150183657 18.89422512636685 10-11 26.415621357183973 29.978802518684088 27.899124361229454 15.706451762902486 12-13 24.039284841558 29.341240841971043 27.65777657199335 18.961697744477604 14-15 23.344615650539243 29.672339252634806 27.85622236977406 19.12682272705189 16-17 24.965638350615173 27.551629059661316 28.11033055810502 19.372402031618492 18-19 22.093998928583396 27.667818522359045 30.531749415507125 19.706433133550426 20-21 24.022769443113592 27.707515179735637 30.491915151258603 17.777800225892168 22-23 21.619137594755095 32.496531766326676 27.935315437696538 17.94901520122169 24-25 21.336628075151157 27.906084785886677 30.425244251518933 20.33204288744323 26-27 21.31512598843858 27.877351199466887 28.122612883550087 22.684909928544446 28-29 23.357824226133324 30.32846080976121 28.02930889952288 18.284406064582583 30-31 20.912102163292715 28.10311509276523 30.400390982015214 20.58439176192684 32-33 20.883368576872925 30.441887924902723 28.037117636457275 20.637625861767077 34-35 20.738353757910556 28.188850855369363 30.40412698962448 20.668668397095598 36-37 23.042299944007986 28.07436547197802 28.134670727851248 20.748663856162743 38-39 22.98537535540221 30.415245752650964 28.060788288996115 18.538590602950713 40-41 22.79455375890209 30.36729176738268 28.077949586272638 18.760204887442587 42-43 20.563925501556714 30.29694543426612 30.281087264350244 18.85804179982692 44-45 20.355977667856262 28.074049024696425 30.513241972231214 21.0567313352161 46-47 22.682127647701556 28.03607547987912 28.075393930142084 21.206402942277244 48-49 20.33228095477017 30.41529587263317 30.223084051261402 19.029339121335255 50-51 22.576730383418198 28.074134285126462 30.169277571246976 19.17985776020836 52-53 20.35894691032613 28.175485227426154 27.847408918736814 23.618158943510903 54-55 22.362910882451054 28.10517756793513 30.386842139026456 19.14506941058736 56-57 20.169040582600843 28.116330941216333 30.278601369265278 21.436027106917543 58-59 20.083892846174265 28.371258320523275 30.259448770595327 21.285400062707136 60-61 22.25455772346668 28.20626593166654 30.279467248919502 19.25970909594728 62-63 20.03922562818011 28.315348907168097 30.14703276611555 21.498392698536247 64-65 22.180368956255382 28.31758583933082 30.19283630811537 19.309208896298426 66-67 20.088322562608596 32.457715729535295 27.88055201842221 19.573409689433902 68-69 20.03595116774731 32.67015453195795 27.78235797572502 19.51153632456972 70-71 19.931793366719646 32.61581790134596 27.892249495572152 19.560139236362243 72-73 19.855740891779202 32.58268553872212 27.814548292075774 19.747025277422907 74-75 19.820506935097853 32.27636709398604 28.04360996251953 19.859516008396575 76-77 19.94724146078213 31.907479756404783 27.99311117067272 20.152167612140367 78-79 19.874069784430066 31.746268994214915 28.124468267827673 20.255192953527352 80-81 19.89636765670323 31.400038804002904 28.214871553939968 20.488721985353894 82-83 19.930654567774386 31.375264486890618 28.015743824684076 20.67833712065092 84-85 19.942821306239225 31.12356662405017 28.251033996612378 20.682578073098224 86-87 20.085271041815624 30.87693518239624 28.209195851380812 20.828597924407315 88-89 20.131085646739177 31.00165120119073 28.130020666078043 20.737242485992056 90-91 20.192066807184837 30.670400630760007 28.14811440800973 20.989418154045424 92-93 20.133315625071177 30.7194238142121 28.05166770446065 21.09559285625607 94-95 20.066910499588026 30.67460495040818 28.164563002227734 21.093921547776063 96-97 20.147498336276897 30.590530676744336 28.07580257697837 21.1861684100004 98-99 20.17972948023202 30.549183392691898 28.02088240780976 21.250204719266325 100-101 20.291850617700167 30.499436879681507 27.909805158124378 21.298907344493948 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5.0 1 2.5 2 3.5 3 9.5 4 9.0 5 4.5 6 7.0 7 13.5 8 16.0 9 15.5 10 21.0 11 27.5 12 34.5 13 41.0 14 56.5 15 110.0 16 192.0 17 316.5 18 534.5 19 949.5 20 1642.5 21 2705.0 22 4190.5 23 6294.0 24 9199.5 25 12959.5 26 17904.0 27 23755.5 28 30350.5 29 37750.5 30 45848.0 31 54859.0 32 64389.5 33 75357.5 34 87313.5 35 99995.5 36 113969.5 37 127833.0 38 140230.5 39 149514.5 40 156293.5 41 159313.0 42 158937.0 43 157691.5 44 153227.0 45 145597.5 46 137274.5 47 127697.0 48 115759.0 49 103895.5 50 93628.5 51 84203.0 52 74228.0 53 63688.0 54 53734.5 55 44798.0 56 37235.0 57 30706.0 58 25128.5 59 20346.5 60 16227.0 61 12529.5 62 9630.5 63 7381.5 64 5475.5 65 4038.0 66 2952.5 67 2193.5 68 1597.5 69 1148.0 70 841.5 71 616.0 72 459.0 73 348.5 74 262.0 75 181.0 76 147.5 77 119.0 78 89.5 79 57.5 80 46.0 81 41.5 82 27.0 83 17.5 84 14.0 85 13.5 86 11.0 87 10.5 88 7.5 89 4.0 90 1.5 91 0.5 92 0.5 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17006050087515576 2 0.006830640521668523 3 0.0020523990299849085 4 0.0 5 0.0012506806588970536 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.2448114390459937E-4 12-13 0.0 14-15 1.6034367421757098E-5 16-17 0.0 18-19 1.6034367421757098E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 1.2827493937405678E-4 40-41 1.443093067958139E-4 42-43 0.0011384400869447538 44-45 5.291341249179842E-4 46-47 0.005547891127927956 48-49 0.004088763692548059 50-51 0.008257699222204904 52-53 0.004008591855439275 54-55 0.01994675307266583 56-57 0.011304229032338752 58-59 0.020347612258209757 60-61 0.00955648298336723 62-63 0.006012887783158912 64-65 0.012779390835140406 66-67 0.03137925704437864 68-69 0.03264597207069745 70-71 0.03708749184652417 72-73 0.03189235680187487 74-75 0.03458613052873006 76-77 0.0346823367332606 78-79 0.017429357387449967 80-81 0.002148605234515451 82-83 0.0 84-85 0.005852544108941341 86-87 0.026649118654960297 88-89 0.028044108620653162 90-91 0.025430506730906757 92-93 0.0356925018808313 94-95 0.032180975415466495 96-97 0.0335599310137376 98-99 0.03939644075525719 100-101 0.02729049335183058 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3118302.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.61956480505879 #Duplication Level Percentage of deduplicated Percentage of total 1 97.73800330407512 87.59237321026612 2 1.5839271277742868 2.8390171974811667 3 0.2632270102225382 0.707708703032419 4 0.11727092029985117 0.4203907536624559 5 0.07783816676631944 0.348791131541057 6 0.03807538822729068 0.204737983362807 7 0.01922341013802215 0.12059555554470901 8 0.023580231366237183 0.16906000584358186 9 0.008994976530796173 0.07255132939095034 >10 0.11313298939539848 2.088361975485067 >50 0.01298802770657794 0.7864618566299069 >100 0.003383993359933405 0.5047042112090967 >500 2.4517400208952584E-4 0.1722511842876979 >1k 7.345489361824855E-5 0.13618068068589276 >5k 0.0 0.0 >10k+ 3.582524198873369E-5 3.836814221577029 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 119503 3.8323100200044773 TruSeq Adapter, Index 6 (97% over 36bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.001827917886080309 0.0 0.0 2 0.0020203302951413943 0.0 0.0 3 0.00259756752232465 0.0 0.0 4 0.0027899799313857348 0.0 0.0 5 0.0035275608327865614 0.0 0.0 6 0.004136866794813331 0.0 6.413746968702839E-5 7 0.005323409984023356 0.0 6.413746968702839E-5 8 0.00634960949901581 0.0 6.413746968702839E-5 9 0.00718339660494718 0.0 6.413746968702839E-5 10-11 0.010807163642264283 0.0 6.413746968702839E-5 12-13 0.013773521615289348 0.0 6.413746968702839E-5 14-15 0.01667574211862738 0.0 6.413746968702839E-5 16-17 0.01927330964095203 0.0 6.413746968702839E-5 18-19 0.022335873818507636 0.0 6.413746968702839E-5 20-21 0.026889634166286654 0.0 1.1224057195229969E-4 22-23 0.03145942888148742 0.0 1.6034367421757098E-4 24-25 0.03695921690715011 0.0 1.6034367421757098E-4 26-27 0.04199400827758184 0.0 1.9241240906108518E-4 28-29 0.04805499916300602 0.0 2.5654987874811357E-4 30-31 0.08039631825269009 0.0 2.5654987874811357E-4 32-33 0.0895519420505134 0.0 2.5654987874811357E-4 34-35 0.09755309139397018 0.0 2.5654987874811357E-4 36-37 0.10795939585069053 3.2068734843514196E-5 2.5654987874811357E-4 38-39 0.12022568692833471 3.2068734843514196E-5 2.8861861359162774E-4 40-41 0.13181853457426512 3.2068734843514196E-5 2.8861861359162774E-4 42-43 0.14591274353798958 3.2068734843514196E-5 2.8861861359162774E-4 44-45 0.15952592147906136 3.2068734843514196E-5 3.3672171585689904E-4 46-47 0.17387668032153397 3.2068734843514196E-5 3.5275608327865613E-4 48-49 0.19111362529992285 3.2068734843514196E-5 3.687904507004132E-4 50-51 0.20878349819869918 3.2068734843514196E-5 3.8482481812217035E-4 52-53 0.2264533710974755 3.2068734843514196E-5 4.0085918554392744E-4 54-55 0.24564650890131873 3.2068734843514196E-5 4.4896228780919874E-4 56-57 0.26820686386373094 3.2068734843514196E-5 4.4896228780919874E-4 58-59 0.2911360092768436 3.2068734843514196E-5 4.4896228780919874E-4 60-61 0.3167108253145462 3.2068734843514196E-5 4.810310226527129E-4 62-63 0.3441616623405943 3.2068734843514196E-5 4.810310226527129E-4 64-65 0.3740497232147496 3.2068734843514196E-5 4.810310226527129E-4 66-67 0.40889240362222773 3.2068734843514196E-5 5.451684923397413E-4 68-69 0.4449697303211812 3.2068734843514196E-5 5.451684923397413E-4 70-71 0.48418979303479903 3.2068734843514196E-5 5.451684923397413E-4 72-73 0.5284767158536922 3.2068734843514196E-5 5.612028597614983E-4 74-75 0.5781672204937174 3.2068734843514196E-5 5.772372271832555E-4 76-77 0.6329566539738614 3.2068734843514196E-5 5.772372271832555E-4 78-79 0.6965329208011284 3.2068734843514196E-5 5.932715946050126E-4 80-81 0.7622417584954888 3.2068734843514196E-5 6.093059620267698E-4 82-83 0.8349255460183138 3.2068734843514196E-5 6.734434317137981E-4 84-85 0.9185447721227771 3.2068734843514196E-5 7.536152688225836E-4 86-87 1.007327064537046 3.2068734843514196E-5 7.696496362443407E-4 88-89 1.1063072146315527 3.2068734843514196E-5 7.696496362443407E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 18840 0.0 70.8872 4 TCGGAAG 18980 0.0 70.48944 3 ATCGGAA 19280 0.0 69.41947 2 GATCGGA 19385 0.0 69.35847 1 GAAGAGC 19880 0.0 67.104996 6 AGAGCAC 19820 0.0 67.06853 8 GAGCACA 19920 0.0 66.70799 9 AAGAGCA 21490 0.0 61.90079 7 GGAAGAG 23180 0.0 57.901806 5 GGCGTCG 25 0.0050241984 56.993927 2 GCCGTCT 14890 0.0 44.151398 50-51 CGTCTTC 15015 0.0 43.99053 52-53 TCTCGTA 14645 0.0 43.943058 42-43 ATGCCGT 14925 0.0 43.886967 48-49 TGCCGTC 14870 0.0 43.779537 50-51 TCGTATG 15145 0.0 43.73242 44-45 TATGCCG 14925 0.0 43.58463 48-49 CCGTCTT 14985 0.0 43.5397 52-53 CTCGTAT 14675 0.0 43.46625 44-45 CGTATGC 14995 0.0 43.458614 46-47 >>END_MODULE