FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n01_52150223-1.3410000003573e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n01_52150223-1.3410000003573e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3118302
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT1195033.8323100200044773TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA188400.070.88724
TCGGAAG189800.070.489443
ATCGGAA192800.069.419472
GATCGGA193850.069.358471
GAAGAGC198800.067.1049966
AGAGCAC198200.067.068538
GAGCACA199200.066.707999
AAGAGCA214900.061.900797
GGAAGAG231800.057.9018065
GGCGTCG250.005024198456.9939272
GCCGTCT148900.044.15139850-51
CGTCTTC150150.043.9905352-53
TCTCGTA146450.043.94305842-43
ATGCCGT149250.043.88696748-49
TGCCGTC148700.043.77953750-51
TCGTATG151450.043.7324244-45
TATGCCG149250.043.5846348-49
CCGTCTT149850.043.539752-53
CTCGTAT146750.043.4662544-45
CGTATGC149950.043.45861446-47
Produced by FastQC (version 0.11.2)