##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n01_50331580-1.341000000357a8.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3064911 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.69561661007449 33.0 33.0 33.0 33.0 33.0 2 32.72172014130263 33.0 33.0 33.0 33.0 33.0 3 32.75205022266552 33.0 33.0 33.0 33.0 33.0 4 36.693219150572396 37.0 37.0 37.0 37.0 37.0 5 36.655063067084164 37.0 37.0 37.0 37.0 37.0 6 36.53699895364009 37.0 37.0 37.0 37.0 37.0 7 36.48038523794003 37.0 37.0 37.0 37.0 37.0 8 36.41616444979969 37.0 37.0 37.0 37.0 37.0 9 36.44430556058561 37.0 37.0 37.0 37.0 37.0 10-11 36.3816025979221 37.0 37.0 37.0 37.0 37.0 12-13 36.35183745955429 37.0 37.0 37.0 37.0 37.0 14-15 38.17409820383039 40.0 37.0 40.0 37.0 40.0 16-17 38.160789334502695 40.0 37.0 40.0 37.0 40.0 18-19 38.15684892644518 40.0 37.0 40.0 37.0 40.0 20-21 38.09754149467962 40.0 37.0 40.0 37.0 40.0 22-23 37.99918056348129 40.0 37.0 40.0 33.0 40.0 24-25 37.970030614265795 40.0 37.0 40.0 33.0 40.0 26-27 37.86984825986791 40.0 37.0 40.0 33.0 40.0 28-29 37.73451512947685 40.0 37.0 40.0 33.0 40.0 30-31 37.67309474891766 40.0 37.0 40.0 33.0 40.0 32-33 37.55222207104872 40.0 37.0 40.0 33.0 40.0 34-35 37.47029685364436 40.0 37.0 40.0 33.0 40.0 36-37 37.4073805079495 40.0 37.0 40.0 33.0 40.0 38-39 37.288069049965884 40.0 37.0 40.0 33.0 40.0 40-41 37.20042588512358 40.0 37.0 40.0 33.0 40.0 42-43 37.06426255118012 40.0 37.0 40.0 33.0 40.0 44-45 36.97241339144921 38.5 37.0 40.0 33.0 40.0 46-47 36.884434817193714 37.0 37.0 40.0 33.0 40.0 48-49 36.64904837367219 37.0 37.0 40.0 33.0 40.0 50-51 36.51729968667932 37.0 37.0 40.0 33.0 40.0 52-53 36.411612278464204 37.0 37.0 40.0 33.0 40.0 54-55 36.21044395742649 37.0 37.0 40.0 33.0 40.0 56-57 36.030080481945475 37.0 37.0 40.0 30.0 40.0 58-59 35.80062357438764 37.0 37.0 40.0 27.0 40.0 60-61 35.569648351942355 37.0 35.0 40.0 27.0 40.0 62-63 35.35670138545622 37.0 33.0 40.0 27.0 40.0 64-65 35.085953066826406 37.0 33.0 40.0 27.0 40.0 66-67 34.70614105923467 37.0 33.0 37.0 27.0 40.0 68-69 34.42731338691401 37.0 33.0 37.0 27.0 40.0 70-71 34.12191789582144 37.0 33.0 37.0 27.0 40.0 72-73 33.6468179663292 37.0 33.0 37.0 27.0 40.0 74-75 33.11192723051338 37.0 33.0 37.0 22.0 38.5 76-77 30.506759576379217 33.0 30.0 35.0 22.0 37.0 78-79 32.801787882258246 35.0 33.0 37.0 24.5 37.0 80-81 33.131155684455436 37.0 33.0 37.0 27.0 37.0 82-83 33.02016143372516 37.0 33.0 37.0 27.0 37.0 84-85 32.855925995893514 37.0 33.0 37.0 27.0 37.0 86-87 32.659633183475805 37.0 33.0 37.0 22.0 37.0 88-89 32.48370621528651 37.0 33.0 37.0 22.0 37.0 90-91 32.29938177650183 33.0 33.0 37.0 22.0 37.0 92-93 32.117865086457655 33.0 33.0 37.0 22.0 37.0 94-95 32.00694636810009 33.0 33.0 37.0 22.0 37.0 96-97 31.867899589906525 33.0 33.0 37.0 22.0 37.0 98-99 31.563500049430473 33.0 33.0 37.0 18.5 37.0 100-101 29.915512065440073 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.13525681443950077 2306 2 0.1294113162711028 2306 3 0.12562835873349343 2306 4 0.14738813720445165 2306 5 0.20364264844079827 2306 6 0.21377691517690778 2306 7 0.2950665434996438 2306 8 0.2819280037615428 2306 9 0.2495151860491518 2306 10-11 0.25071340561820676 2306 12-13 0.2949457934188331 2306 14-15 0.5073058057057764 2306 16-17 0.6221778690051423 2306 18-19 0.4637075186844939 2306 20-21 0.5547170180744772 2306 22-23 0.6039628979014466 2306 24-25 0.6356007507079156 2306 26-27 0.6635230113684543 2306 28-29 0.6313003801010737 2306 30-31 0.6625571700156243 2306 32-33 0.6597761471264363 2306 34-35 0.8023901122257442 2306 36-37 0.6666289392163023 2306 38-39 0.8392277408093349 2306 40-41 0.753937716655031 2306 42-43 0.7996942039661477 2306 44-45 0.8484844122711905 2306 46-47 0.7672692888967845 2306 48-49 0.7283842770783266 2306 50-51 0.6861520505045604 2306 52-53 0.7832587352415956 2306 54-55 0.7552887164466071 2306 56-57 0.820757130349044 2306 58-59 0.7115833037286023 2306 60-61 0.6481234082521539 2306 62-63 0.6733809059193518 2306 64-65 0.5892814979035492 2306 66-67 0.6154993684327579 2306 68-69 0.6586626551899926 2306 70-71 0.5148647426350834 2306 72-73 0.4413838487847883 2306 74-75 0.3321219173500154 2306 76-77 -0.01613919686046117 2306 78-79 0.31599013628733985 2306 80-81 0.38317988702328876 2306 82-83 0.5733216399554522 2306 84-85 0.37996822552157283 2306 86-87 0.38531446831540705 2306 88-89 0.3321515335879681 2306 90-91 0.40317526019212835 2306 92-93 0.4895635570367318 2306 94-95 0.5319287945539557 2306 96-97 0.6940179069532313 2306 98-99 0.5197692542886969 2306 100-101 0.3350607906918981 2307 1 0.08688158005525537 2307 2 0.041148516243694644 2307 3 0.09783727841679024 2307 4 0.12673429081100807 2307 5 0.19842779361886898 2307 6 0.25491057182033927 2307 7 0.2784145550491033 2307 8 0.26938182772885 2307 9 0.23600480306062366 2307 10-11 0.28652825296476436 2307 12-13 0.2733070075759585 2307 14-15 0.5133705954296275 2307 16-17 0.49840614758132773 2307 18-19 0.5160076185119351 2307 20-21 0.4988124817659454 2307 22-23 0.4496032535737058 2307 24-25 0.5387114813015188 2307 26-27 0.562446654275881 2307 28-29 0.6267539291925104 2307 30-31 0.6384208487114265 2307 32-33 0.48378219439574366 2307 34-35 0.593631422098845 2307 36-37 0.6513146346133354 2307 38-39 0.6057203907996751 2307 40-41 0.5951527069681788 2307 42-43 0.678870779402061 2307 44-45 0.640806829216622 2307 46-47 0.6841975937925326 2307 48-49 0.6857966204847301 2307 50-51 0.6177513686877916 2307 52-53 0.6429515361411333 2307 54-55 0.683031693484736 2307 56-57 0.7706698592046308 2307 58-59 0.8313012873076815 2307 60-61 0.758264439823165 2307 62-63 0.6511803960362883 2307 64-65 0.5280460826774487 2307 66-67 0.7216473996050823 2307 68-69 0.7766713274071222 2307 70-71 0.5998112197074761 2307 72-73 0.45418757494923767 2307 74-75 0.4538073464367045 2307 76-77 0.20934896089493193 2307 78-79 0.5542259548723791 2307 80-81 0.6648596239211315 2307 82-83 0.6537096938342444 2307 84-85 0.6004077777464332 2307 86-87 0.6413547294552728 2307 88-89 0.7241976340881919 2307 90-91 0.9097522301099588 2307 92-93 0.890682605545706 2307 94-95 0.9752143282959409 2307 96-97 0.8787359567205648 2307 98-99 0.7900544905729348 2307 100-101 0.3733127280606894 2201 1 0.13100213979993924 2201 2 0.09991342010605564 2201 3 0.088943774309449 2201 4 0.12594204025755573 2201 5 0.18562957590167173 2201 6 0.13758093378331893 2201 7 0.17130867160545193 2201 8 0.2207117972749657 2201 9 0.21057306864997827 2201 10-11 0.2350881549729209 2201 12-13 0.2724057172189376 2201 14-15 0.44464075286835225 2201 16-17 0.403055316842007 2201 18-19 0.38051995598819843 2201 20-21 0.3487589627126013 2201 22-23 0.4542916139521864 2201 24-25 0.45232210701089315 2201 26-27 0.48371236584048916 2201 28-29 0.3581741436620334 2201 30-31 0.44727389747135504 2201 32-33 0.5016573475079937 2201 34-35 0.5841191478340662 2201 36-37 0.5423526162848162 2201 38-39 0.4387765886150845 2201 40-41 0.47826660281813815 2201 42-43 0.5238662211519767 2201 44-45 0.3804547202006958 2201 46-47 0.46905380874527225 2201 48-49 0.6433757472111026 2201 50-51 0.5777486857116259 2201 52-53 0.5590484800767612 2201 54-55 0.5678493143169945 2201 56-57 0.4953605092434046 2201 58-59 0.47951570345836103 2201 60-61 0.5676208977889345 2201 62-63 0.671308687247361 2201 64-65 0.583634960828185 2201 66-67 0.5528938176212037 2201 68-69 0.5824925077480785 2201 70-71 0.6613649789644498 2201 72-73 0.5492917053278319 2201 74-75 0.5577524802764842 2201 76-77 0.3637654767905083 2201 78-79 0.5516535987978912 2201 80-81 0.5998808559550213 2201 82-83 0.55445063773616 2201 84-85 0.3822804108276685 2201 86-87 0.5091963804829547 2201 88-89 0.6181296480340492 2201 90-91 0.6510553839414186 2201 92-93 0.8066907022339862 2201 94-95 0.7949437901836305 2201 96-97 0.7810203142671703 2201 98-99 0.8246000913348439 2201 100-101 0.6038200634018054 1101 1 0.08208447726455859 1101 2 0.09613318375814117 1101 3 0.095617388038022 1101 4 0.14051608928608061 1101 5 0.18877422998972548 1101 6 0.10305625323731249 1101 7 0.13800711738511495 1101 8 0.20178474881551267 1101 9 0.20107259196328187 1101 10-11 0.17494987887894098 1101 12-13 0.22046903894919723 1101 14-15 0.3230764075550283 1101 16-17 0.3721649104388831 1101 18-19 0.3545608549675734 1101 20-21 0.2944480890826142 1101 22-23 0.306721977047026 1101 24-25 0.266427229360481 1101 26-27 0.3200499339253895 1101 28-29 0.3187865197789179 1101 30-31 0.2506147691059155 1101 32-33 0.2678166569404823 1101 34-35 0.2537604872867334 1101 36-37 0.2748297611901833 1101 38-39 0.3089570644149262 1101 40-41 0.3744948655353255 1101 42-43 0.4275995278795435 1101 44-45 0.33408677719616975 1101 46-47 0.3273799363495016 1101 48-49 0.3801504628619483 1101 50-51 0.3796798211128163 1101 52-53 0.4272150469718028 1101 54-55 0.4375216938335029 1101 56-57 0.46800091010365463 1101 58-59 0.46469776821384556 1101 60-61 0.4259230062961308 1101 62-63 0.3380474112051175 1101 64-65 0.2599573045008228 1101 66-67 0.3990515011455784 1101 68-69 0.44135444597834805 1101 70-71 0.30792766141720307 1101 72-73 0.2732660898846646 1101 74-75 0.24269952740564804 1101 76-77 0.11293774175441129 1101 78-79 0.3800944268285562 1101 80-81 0.511494274131195 1101 82-83 0.4245498181306644 1101 84-85 0.2921644540177013 1101 86-87 0.4158766456537819 1101 88-89 0.5304488581383282 1101 90-91 0.30071468228816656 1101 92-93 0.49113889119406906 1101 94-95 0.4955291171020164 1101 96-97 0.5102437953364252 1101 98-99 0.33480081708110276 1101 100-101 0.3407481317490735 2304 1 0.07169079615476193 2304 2 0.08677058488147793 2304 3 0.12206750528098098 2304 4 0.11948724025211277 2304 5 0.14855847813714718 2304 6 0.1284810484830743 2304 7 0.14861525650987772 2304 8 0.1332219897156648 2304 9 0.07798565493772713 2304 10-11 0.11866063173291508 2304 12-13 0.21726213836377894 2304 14-15 0.2816031518874027 2304 16-17 0.2545907407547361 2304 18-19 0.3100151780689515 2304 20-21 0.32419239664022115 2304 22-23 0.36539729115712305 2304 24-25 0.35486922732932413 2304 26-27 0.3062969275253238 2304 28-29 0.3189728412568016 2304 30-31 0.40390993422972343 2304 32-33 0.417820733415148 2304 34-35 0.45601300539602363 2304 36-37 0.46583785565096036 2304 38-39 0.552464835416302 2304 40-41 0.40504095362616965 2304 42-43 0.5354209534758994 2304 44-45 0.5624887725419043 2304 46-47 0.5397653741185735 2304 48-49 0.6814699556776063 2304 50-51 0.5173780396933907 2304 52-53 0.48931393515764654 2304 54-55 0.46444924601681237 2304 56-57 0.6447991671448747 2304 58-59 0.5311562688155647 2304 60-61 0.7260197782599107 2304 62-63 0.6681080443348009 2304 64-65 0.49304148547502535 2304 66-67 0.5751722658213296 2304 68-69 0.574707895827963 2304 70-71 0.5373186449641949 2304 72-73 0.45737785264092423 2304 74-75 0.526535153312885 2304 76-77 0.44300435666756854 2304 78-79 0.5811654319276869 2304 80-81 0.6041981820524924 2304 82-83 0.514066764744797 2304 84-85 0.5435987527665773 2304 86-87 0.63560306103237 2304 88-89 0.6431503113129295 2304 90-91 0.6683209763650524 2304 92-93 0.722666479179459 2304 94-95 0.6890194301575505 2304 96-97 0.49864578182024744 2304 98-99 0.5527907428715366 2304 100-101 0.6632444673528255 2202 1 0.07457972095544818 2202 2 0.0855418626233444 2202 3 0.11125948976707889 2202 4 0.13998282310063814 2202 5 0.13834462336446762 2202 6 0.2014125287674986 2202 7 0.11109801884558124 2202 8 0.13455306060556183 2202 9 0.1668829830997609 2202 10-11 0.21239420208413406 2202 12-13 0.206375745345035 2202 14-15 0.24532998877599965 2202 16-17 0.32244357419580894 2202 18-19 0.27748878289550305 2202 20-21 0.2503168848970603 2202 22-23 0.26018666844650085 2202 24-25 0.3025055126126759 2202 26-27 0.15386911024457106 2202 28-29 0.18907168848800637 2202 30-31 0.3117207508640618 2202 32-33 0.3067371846110092 2202 34-35 0.36200470977008337 2202 36-37 0.4005502021280094 2202 38-39 0.41468687807952165 2202 40-41 0.3794408674378502 2202 42-43 0.3661322764666366 2202 44-45 0.39310501904049744 2202 46-47 0.4114016055342091 2202 48-49 0.4458846831070389 2202 50-51 0.43670684813984906 2202 52-53 0.45495496079866626 2202 54-55 0.42221589113640334 2202 56-57 0.44244945803045965 2202 58-59 0.3832826963096494 2202 60-61 0.4559882656548666 2202 62-63 0.42713196159401434 2202 64-65 0.45515713942891267 2202 66-67 0.39281178316672793 2202 68-69 0.45782225695703005 2202 70-71 0.33546802009886534 2202 72-73 0.3759096884521753 2202 74-75 0.2521794110159661 2202 76-77 0.10648447948592832 2202 78-79 0.26874650281758505 2202 80-81 0.309344702199013 2202 82-83 0.4865142437363943 2202 84-85 0.3689033746153214 2202 86-87 0.37002155920087176 2202 88-89 0.3191083483739092 2202 90-91 0.36555702463050466 2202 92-93 0.3189482611230048 2202 94-95 0.4701274887656055 2202 96-97 0.4782210349984446 2202 98-99 0.3528419171874191 2202 100-101 0.21764491984151135 1102 1 0.023660226085681302 1102 2 0.0319151854331281 1102 3 0.036413396633697914 1102 4 0.08330168655762549 1102 5 0.09924044025476064 1102 6 0.05637503863331972 1102 7 0.1310052800926229 1102 8 0.11230167939009306 1102 9 0.04805306991902114 1102 10-11 0.024333823236467822 1102 12-13 0.015820299409746497 1102 14-15 0.08550362729217653 1102 16-17 0.0913484676449059 1102 18-19 0.06270287129328267 1102 20-21 0.1334265928686449 1102 22-23 0.13075888628390686 1102 24-25 0.11965922216278813 1102 26-27 0.07694823415933172 1102 28-29 0.1418775290562806 1102 30-31 0.10839596791220885 1102 32-33 0.03956768974124003 1102 34-35 -0.05188341177687761 1102 36-37 0.13545549021009862 1102 38-39 0.04716418668251521 1102 40-41 0.23683135678275846 1102 42-43 0.08731913518802514 1102 44-45 0.11020841288422645 1102 46-47 0.07543870103539518 1102 48-49 0.06264375966583913 1102 50-51 0.13270822935531612 1102 52-53 0.08556960168264283 1102 54-55 0.03014604109694119 1102 56-57 0.08610375953006866 1102 58-59 -0.007921920896350798 1102 60-61 -0.05274200269555962 1102 62-63 0.06251771534302719 1102 64-65 0.170857612988506 1102 66-67 0.12149272239324915 1102 68-69 0.2191936066018556 1102 70-71 0.03995894354006424 1102 72-73 -0.16315897768831888 1102 74-75 0.0073944859962367104 1102 76-77 -0.1695889222504583 1102 78-79 0.08804208292807658 1102 80-81 0.04393823021796095 1102 82-83 0.13170366757222496 1102 84-85 0.07076604233433414 1102 86-87 0.047001630234809966 1102 88-89 0.14624528602189457 1102 90-91 0.1630846489399005 1102 92-93 0.08468425165180804 1102 94-95 -0.04151517106074465 1102 96-97 0.04856041937097544 1102 98-99 0.09449542942103761 1102 100-101 0.022907350780741353 2305 1 0.04931330723060512 2305 2 0.012027345962174252 2305 3 0.03242742114286301 2305 4 0.010431519900372166 2305 5 0.06712307931959316 2305 6 0.06524459739171817 2305 7 -0.018168724803381053 2305 8 0.0582077950166493 2305 9 0.01673534205720273 2305 10-11 0.031936432371168166 2305 12-13 -0.035652875572161236 2305 14-15 0.02482726690202952 2305 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-0.23823626848258783 1213 64-65 -0.5348211900966859 1213 66-67 -0.44629451974954293 1213 68-69 -0.040922367421416084 1213 70-71 0.2462715075333506 1213 72-73 0.3014363313765145 1213 74-75 0.33856363356669306 1213 76-77 -1.9818720922114217 1213 78-79 -1.7452386806255014 1213 80-81 -1.3159690997093314 1213 82-83 -0.7855431074260792 1213 84-85 -0.4359264533647931 1213 86-87 -0.31603357451771785 1213 88-89 -0.35998333957126505 1213 90-91 -1.6154095793205734 1213 92-93 -0.6496505495207252 1213 94-95 -0.3841256358144207 1213 96-97 -0.2348832184621088 1213 98-99 -2.0559287453579387 1213 100-101 -0.5975643934228323 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 2.0 9 18.0 10 17.0 11 16.0 12 35.0 13 114.0 14 318.0 15 761.0 16 1455.0 17 2515.0 18 3562.0 19 4979.0 20 6578.0 21 8424.0 22 10917.0 23 13839.0 24 17239.0 25 22059.0 26 28068.0 27 37407.0 28 52952.0 29 74794.0 30 75039.0 31 78096.0 32 101606.0 33 141988.0 34 211139.0 35 341917.0 36 595904.0 37 895020.0 38 336859.0 39 1273.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.46143558259751 25.198326060070443 22.09145746775404 16.24878088957801 2 29.04937608534915 24.913547310944494 31.568952341547995 14.468124262158359 3 36.05977829967979 20.143053935940916 27.029767773906656 16.767399990472644 4 28.665302189851516 20.878028758420715 31.747512407374963 18.709156644352806 5 32.55608550302557 19.14496321871633 31.703260884460505 16.59569039379759 6 27.985608717512516 20.212006156133082 35.16607823196171 16.636306894392693 7 31.988432943077306 22.682289958827514 27.168097213915836 18.161179884179347 8 28.45446409373714 30.125050939488947 24.138188678235682 17.28229628853823 9 31.737561058053558 25.137238895354546 24.51278356859302 18.61241647799887 10-11 26.766620825115297 28.963866743014872 27.704007138883586 16.565505292986245 12-13 25.57529402974507 27.931430961616833 27.203530542974985 19.289744465663112 14-15 24.620698610824263 28.39040024326971 27.846061435389153 19.142839710516878 16-17 24.878781145684165 27.575825203407213 28.11706441067946 19.42832924022916 18-19 22.615374902464524 28.00403143909096 29.737638342131035 19.64295531631348 20-21 23.64512378989145 27.9648740208117 29.75769932634259 18.63230286295426 22-23 22.129777993553486 30.97509193578541 28.106248435925217 18.788881634735883 24-25 21.978860071303867 27.96260641826141 29.787520746931968 20.271012763502757 26-27 22.040052712786768 27.898248921420553 28.19008447553616 21.87161389025652 28-29 22.980960947968796 29.770652394147824 28.04407697319759 19.204309684685786 30-31 21.27838296120181 28.402651822516216 29.60585152391048 20.71311369237149 32-33 21.24898569648515 30.005438983383204 28.135384681643284 20.61019063848836 34-35 22.459281851903693 28.542590633137472 28.607160207914685 20.390967307044154 36-37 21.181251266349985 28.439047659132676 29.833199071685932 20.546502002831403 38-39 21.24054416042259 30.016949954403156 28.157365399580087 20.58514048559417 40-41 21.13035945590572 28.27861177000304 30.95767901830723 19.633349755784018 42-43 21.097384944473955 29.715929038379468 29.52512147342071 19.661564543725863 44-45 20.716524549074574 28.25499695503087 30.04561499159106 20.9828635043035 46-47 22.294766211906726 28.141299863071158 28.34706020643577 21.21687371858635 48-49 20.887133389443914 29.982838907112953 29.383513963393476 19.746513740049657 50-51 22.24280280195357 28.244292369677098 29.488840070513824 20.024064757855513 52-53 20.803293807405193 28.413787410293363 28.010088157997558 22.772830624303886 54-55 22.029773125669102 28.33253950732531 29.725006596180137 19.91268077082545 56-57 20.90204228539394 28.167333810400656 29.42539492657503 21.505228977630374 58-59 20.717440979340566 28.51647017589204 29.67333775853663 21.092751086230766 60-61 21.913707043023788 28.359884317956592 29.74963837124956 19.97677026777006 62-63 20.60955956512844 28.647991122193506 29.27664541078943 21.465803901888624 64-65 21.875102993660647 28.51006474446641 29.532806071054125 20.082026190818823 66-67 20.835885206738872 30.825406818801564 27.92186037160499 20.416847602854574 68-69 20.839146345941227 31.127921635503913 27.86100990979597 20.17192210875889 70-71 20.606835549426563 31.047233589418237 28.10952219697186 20.236408664183337 72-73 20.554486661673053 31.196247284927942 27.857740831583122 20.39152522181588 74-75 20.56447519890908 30.8279454814339 28.21099816994181 20.39658114971521 76-77 20.92906742389007 30.203813860125823 28.161872822370732 20.705245893613373 78-79 20.71906730234077 30.21901647604221 28.43645741841237 20.62545880320465 80-81 20.477325162350006 30.07557681032123 28.53027036744954 20.91682765987923 82-83 20.40840663888772 30.19622755766807 28.238813459836194 21.15655234360802 84-85 20.30818014546933 29.95145366546361 28.670366211907783 21.069999977159274 86-87 20.53211960125951 29.67194051790311 28.646695482577293 21.14924439826008 88-89 20.59255246150294 30.168870597815133 28.406617749648543 20.831959191033388 90-91 20.767607625294477 29.574067876236725 28.383013366278387 21.275311132190406 92-93 20.543131131191902 29.909584222550468 28.18742012549487 21.359864520762763 94-95 20.2356840871709 29.95857063382997 28.521736831566542 21.284008447432583 96-97 20.490353800073045 29.9052433719808 28.219895954402453 21.384506873543703 98-99 20.54287476866132 29.819645590774524 28.044678149551682 21.592801491012473 100-101 20.603246238053757 29.533387140307287 28.06638557799036 21.796981043648596 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 68.0 1 47.0 2 44.0 3 78.5 4 82.5 5 57.0 6 52.0 7 56.0 8 57.0 9 59.5 10 60.0 11 68.5 12 67.0 13 73.5 14 92.0 15 114.5 16 164.0 17 229.0 18 355.0 19 587.5 20 900.0 21 1430.0 22 2236.5 23 3422.0 24 4958.0 25 7027.0 26 9832.5 27 13297.5 28 17472.0 29 22514.0 30 28547.0 31 35446.5 32 44028.0 33 54927.5 34 68177.0 35 84689.5 36 105405.0 37 130439.0 38 155639.0 39 172578.5 40 180637.5 41 178191.5 42 169753.0 43 165808.0 44 160604.0 45 154365.0 46 151604.5 47 142756.0 48 125442.0 49 109246.0 50 97052.0 51 86020.0 52 74967.0 53 63517.0 54 52116.5 55 42210.0 56 33490.0 57 26052.5 58 20527.5 59 16226.0 60 12729.5 61 9702.5 62 7109.0 63 5226.0 64 3803.5 65 2786.5 66 2078.5 67 1484.0 68 1078.0 69 788.0 70 565.0 71 425.0 72 315.5 73 231.0 74 168.5 75 120.0 76 91.0 77 68.5 78 49.5 79 37.5 80 31.0 81 19.5 82 15.0 83 11.5 84 8.5 85 9.0 86 8.5 87 5.5 88 3.0 89 4.0 90 4.0 91 2.5 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17302296869305506 2 0.007471668834755724 3 0.0017292508656858224 4 0.0 5 0.0012072128684976497 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.957642489455648E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 1.6313687412130403E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 1.957642489455648E-4 40-41 3.2627374824260806E-5 42-43 0.0014029771174432146 44-45 6.362338090730856E-4 46-47 0.0058892411557790745 48-49 0.004225245039741774 50-51 0.008597313266192721 52-53 0.004225245039741774 54-55 0.020799951450466262 56-57 0.011550090687788324 58-59 0.02104465676164822 60-61 0.009918721946575284 62-63 0.006199201216609552 64-65 0.013116204679352842 66-67 0.03215427788930902 68-69 0.03339411813263093 70-71 0.037472539985663536 72-73 0.03249686532496376 74-75 0.0350418005612561 76-77 0.03543332905914723 78-79 0.018026624590404095 80-81 0.0026754447355893858 82-83 0.0 84-85 0.0068517487130947685 86-87 0.02815742447333707 88-89 0.028663148783113115 90-91 0.026738133668481726 92-93 0.03655897349058423 94-95 0.03332886338298241 96-97 0.03465027206336497 98-99 0.03983802466042244 100-101 0.028451070846755418 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3064911.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.09110806827768 #Duplication Level Percentage of deduplicated Percentage of total 1 94.53627209838527 72.87905968708723 2 3.267829706143979 5.038412260501472 3 0.892220238928973 2.0634674057993396 4 0.34085353708678634 1.0510710745204856 5 0.21410254897054976 0.8252701370191184 6 0.12169891627473635 0.5629142583796786 7 0.07556454247847665 0.40777480172367414 8 0.07010459676020613 0.4323552835939264 9 0.053734064559953006 0.37281767201452326 >10 0.3471772352239258 5.219166248249311 >50 0.04115789272138456 2.214779777822856 >100 0.03729094383302999 5.351229364389775 >500 0.0017315283303793354 0.9041221291364245 >1k 2.1965136485521905E-4 0.2219148834704269 >5k 0.0 0.0 >10k+ 4.249893746331355E-5 2.45564501629169 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 74952 2.4454869978279956 TruSeq Adapter, Index 2 (97% over 37bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0012072128684976497 0.0 0.0 2 0.0012072128684976497 0.0 0.0 3 0.0015987413663887792 0.0 0.0 4 0.0017618782405100833 0.0 0.0 5 0.0022186614880497346 0.0 0.0 6 0.0026101899859408643 0.0 0.0 7 0.0030343458586562545 0.0 0.0 8 0.0035563838558444276 0.0 0.0 9 0.004045794478208339 0.0 0.0 10-11 0.005742417969069901 0.0 6.52547496485216E-5 12-13 0.007014885587216073 0.0 9.78821244727824E-5 14-15 0.008466803766895677 0.0 9.78821244727824E-5 16-17 0.009869780884338892 0.0 9.78821244727824E-5 18-19 0.011843737061206672 0.0 9.78821244727824E-5 20-21 0.013507733177243972 0.0 9.78821244727824E-5 22-23 0.015318552479990448 0.0 9.78821244727824E-5 24-25 0.01709674440791266 0.0 9.78821244727824E-5 26-27 0.018972818460307656 0.0 9.78821244727824E-5 28-29 0.021142538886121 0.0 9.78821244727824E-5 30-31 0.035987994431159666 0.0 1.305094992970432E-4 32-33 0.038679752854161184 0.0 1.305094992970432E-4 34-35 0.040800532217738134 0.0 1.305094992970432E-4 36-37 0.043410722203678996 0.0 1.6313687412130402E-4 38-39 0.04675502812316573 0.0 1.6313687412130402E-4 40-41 0.049707805544761334 0.0 2.2839162376982563E-4 42-43 0.053313130462842154 0.0 2.2839162376982563E-4 44-45 0.05750574812775966 0.0 2.610189985940864E-4 46-47 0.06328079347165383 0.0 2.610189985940864E-4 48-49 0.06851748713094769 0.0 2.9364637341834724E-4 50-51 0.07406414085107202 0.0 2.9364637341834724E-4 52-53 0.081193222250173 0.0 3.2627374824260803E-4 54-55 0.0881102257129163 0.0 3.425874356547384E-4 56-57 0.09647914735533919 0.0 3.5890112306686883E-4 58-59 0.10664257461309642 0.0 3.5890112306686883E-4 60-61 0.1176706273036966 0.0 3.5890112306686883E-4 62-63 0.1295796191145518 0.0 3.915284978911296E-4 64-65 0.1429079017302623 0.0 3.915284978911296E-4 66-67 0.15817751314801637 0.0 3.915284978911296E-4 68-69 0.17602468717688702 0.0 3.915284978911296E-4 70-71 0.1971998534378323 0.0 3.915284978911296E-4 72-73 0.22041423062529386 0.0 4.2415587271539047E-4 74-75 0.2466955810462359 0.0 4.5678324753965126E-4 76-77 0.27387418427484517 0.0 4.89410622363912E-4 78-79 0.306501559099106 0.0 5.383516846003032E-4 80-81 0.3434357474001692 0.0 5.546653720124336E-4 82-83 0.38358373212142216 0.0 5.872927468366945E-4 84-85 0.43146440467602487 0.0 6.036064342488249E-4 86-87 0.4849896130752247 0.0 6.199201216609552E-4 88-89 0.5419733232057962 0.0 6.199201216609552E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 11030 0.0 73.49855 3 CGGAAGA 10995 0.0 73.42775 4 GATCGGA 11175 0.0 72.67292 1 ATCGGAA 11335 0.0 71.52903 2 CGGTCGA 20 0.0020748489 71.24618 2 GAAGAGC 12100 0.0 66.79848 6 CGGTTCG 25 0.0050231377 56.996944 2 GGAAGAG 15295 0.0 53.125996 5 AGAGCAC 16080 0.0 50.146893 8 GAGCACA 16285 0.0 49.428154 9 TTGTGTT 22570 0.0 48.903854 1 AAGAGCA 17250 0.0 46.77316 7 GCCGTCT 9060 0.0 44.67122 50-51 TCTCGTA 9055 0.0 44.478424 42-43 CTCGTAT 8985 0.0 44.40352 44-45 TCGTATG 9100 0.0 44.3904 44-45 ATGCCGT 9090 0.0 44.28718 48-49 CGTATGC 9035 0.0 44.265453 46-47 TGCCGTC 9065 0.0 44.201164 50-51 TATGCCG 9045 0.0 44.139904 48-49 >>END_MODULE