FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n01_50331580-1.341000000357a8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n01_50331580-1.341000000357a8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3064911
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT749522.4454869978279956TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG110300.073.498553
CGGAAGA109950.073.427754
GATCGGA111750.072.672921
ATCGGAA113350.071.529032
CGGTCGA200.002074848971.246182
GAAGAGC121000.066.798486
CGGTTCG250.005023137756.9969442
GGAAGAG152950.053.1259965
AGAGCAC160800.050.1468938
GAGCACA162850.049.4281549
TTGTGTT225700.048.9038541
AAGAGCA172500.046.773167
GCCGTCT90600.044.6712250-51
TCTCGTA90550.044.47842442-43
CTCGTAT89850.044.4035244-45
TCGTATG91000.044.390444-45
ATGCCGT90900.044.2871848-49
CGTATGC90350.044.26545346-47
TGCCGTC90650.044.20116450-51
TATGCCG90450.044.13990448-49
Produced by FastQC (version 0.11.2)