FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n01_50330665-2.34100000035758.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n01_50330665-2.34100000035758.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2433434
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT205340.8438281046455338TruSeq Adapter, Index 6 (97% over 36bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC71770.2949330041414725No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG65070.2673998966070171No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA34380.14128182642307124No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACGG200.002062272571.355031
TCGGAAG36950.060.7884143
CGGAAGA37600.060.1164364
GATCGGA37950.060.042261
ATCGGAA38600.058.4371952
CGTTCGG250.00499275657.0840151
TCCGCGG250.00502701256.985492
GAAGAGC42550.052.788066
CGACGGG654.3710315E-851.2292481
CGAGCGT405.35038E-447.5700151
GGGGCCG1103.45608E-1143.1708262
ATGCCGT25700.043.16114848-49
GCCGTCT25950.043.0212650-51
TATGCCG25350.043.00747348-49
TCGTATG26200.042.8739244-45
TGCCGTC25700.042.7927850-51
AATCTCG25400.042.3499840-41
CACTCCG26800.042.25982730-31
GGGCGCG459.6392666E-442.2114752
CGTATGC26200.042.06159646-47
Produced by FastQC (version 0.11.2)